Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay ...Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.展开更多
Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of the...Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of their excel-lent sensitivity and specificity.However,these assays have limitations in that they cannot differentiate between types of SEs and do not provide rapid,on-site,quantitative testing.In this study,a time-resolved fluorescence immuno-chromatography assay(TRFICA)was developed specifically for detecting staphylococcal enterotoxin E(SEE),which is commonly found in dairy products.Compared with a double antibody sandwich enzyme-linked immunosorbent assay,which had a detection limit of 0.028 ng/mL,TRFICA demonstrated comparable sensitivity,enabling SEE quan-tification with a detection limit as low as 0.081 ng/mL in infant formula.Validation by spiking infant formula samples confirmed no cross-reactivity with analogs(recoveries ranged from 93.17%to 128.77%).Furthermore,with an 8-min reaction time and interpretation delivered by a portable TRFICA strip reader,our method demonstrates potential for use in mobile and on-site detection.This study describes a rapid,easy,and reliable method for detecting trace lev-els of SEE in infant formula,which could serve as an early screening tool toward preventing food poisoning in infants and children.展开更多
基金This work was financially supported by Major Science and Technology Project of Yunnan Province(202302AE090022)Key Research and Development Program of Yunnan(202203AC100010)+4 种基金the National Natural Science Foundation of China(32160597,32160236,32371463)National Key Research and Development Program of China(2022YFC2601604)Cardiovascular Ultrasound Innovation Team of Yunnan Province(202305AS350021)Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)the second phase of“Double-First Class”Program Construction of Yunnan University.
文摘Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.
基金supported by Beijing Municipal Science and Technology Commission(Z211100007021007)the Key R&D Program of Ningxia Hui Autonomous Region(2021BBF02036).
文摘Staphylococcal food poisoning is a significant foodborne illness caused by staphylococcal enterotoxins(SEs).Immu-noassays have become the primary method for rapidly detecting harmful bacteria and toxins because of their excel-lent sensitivity and specificity.However,these assays have limitations in that they cannot differentiate between types of SEs and do not provide rapid,on-site,quantitative testing.In this study,a time-resolved fluorescence immuno-chromatography assay(TRFICA)was developed specifically for detecting staphylococcal enterotoxin E(SEE),which is commonly found in dairy products.Compared with a double antibody sandwich enzyme-linked immunosorbent assay,which had a detection limit of 0.028 ng/mL,TRFICA demonstrated comparable sensitivity,enabling SEE quan-tification with a detection limit as low as 0.081 ng/mL in infant formula.Validation by spiking infant formula samples confirmed no cross-reactivity with analogs(recoveries ranged from 93.17%to 128.77%).Furthermore,with an 8-min reaction time and interpretation delivered by a portable TRFICA strip reader,our method demonstrates potential for use in mobile and on-site detection.This study describes a rapid,easy,and reliable method for detecting trace lev-els of SEE in infant formula,which could serve as an early screening tool toward preventing food poisoning in infants and children.
