Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a n...Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.展开更多
Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of s...Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of stained tongue coating from healthy students at Hunan University of Chinese Medicine and 1007 images of pathological(non-stained)tongue coat-ing from hospitalized patients at The First Hospital of Hunan University of Chinese Medicine withlungcancer;diabetes;andhypertensionwerecollected.Thetongueimageswererandomi-zed into the training;validation;and testing datasets in a 7:2:1 ratio.A deep learning model was constructed using the ResNet50 for recognizing stained tongue coating in the training and validation datasets.The training period was 90 epochs.The model’s performance was evaluated by its accuracy;loss curve;recall;F1 score;confusion matrix;receiver operating characteristic(ROC)curve;and precision-recall(PR)curve in the tasks of predicting stained tongue coating images in the testing dataset.The accuracy of the deep learning model was compared with that of attending physicians of traditional Chinese medicine(TCM).Results The training results showed that after 90 epochs;the model presented an excellent classification performance.The loss curve and accuracy were stable;showing no signs of overfitting.The model achieved an accuracy;recall;and F1 score of 92%;91%;and 92%;re-spectively.The confusion matrix revealed an accuracy of 92%for the model and 69%for TCM practitioners.The areas under the ROC and PR curves were 0.97 and 0.95;respectively.Conclusion The deep learning model constructed using ResNet50 can effectively recognize stained coating images with greater accuracy than visual inspection of TCM practitioners.This model has the potential to assist doctors in identifying false tongue coating and prevent-ing misdiagnosis.展开更多
An innovative, cost-effective, user-friendly and portable device （dew sensor） to directly detect condensation on glass surfaces was developed within the EC-VIDRIO project （contract No. EVK4-CT-2001-00045）, aimed a...An innovative, cost-effective, user-friendly and portable device （dew sensor） to directly detect condensation on glass surfaces was developed within the EC-VIDRIO project （contract No. EVK4-CT-2001-00045）, aimed at finding sustainable solutions to preserve ancient stained glass windows. The results of the research showed that the direct survey of condensation with the new sensor is more accurate and reliable than the traditional indirect microclimatic measurements. Since the construction of the first prototype, the dew sensor was developed further, continuously improved, validated in the laboratory and applied successfully on different surfaces at sites of interest in the field of Cultural Heritage; on the stained glass windows in the Saint Urbain Basilica of Troyes （France）, Sainte Chapelle of Paris （France） and Cologne Cathedral （Germany）; on stone walls in Petrarca＇s Tomb （Padua, Italy） and the Hagar Qim Temple （Malta）. Now the sensor is being used in the Lascaux Caves （France）. The newly built device was patented （PTC/EP2005/050665） and gave such interesting results that the Italian Ministry of University and Research financially supported a spin-off project that has lead to the creation of a new company （R.E.D.s.r.l.） in order to develop the prototype further and to produce the sensor at industrial scale.展开更多
Background: Meconium stained amniotic fluid (MSAF) is frequently encountered in obstetric practice. Literature on the subject is still poorly documented in the African setting. Objective: The aim of this study was to ...Background: Meconium stained amniotic fluid (MSAF) is frequently encountered in obstetric practice. Literature on the subject is still poorly documented in the African setting. Objective: The aim of this study was to determine the maternal and fetal outcomes in case of meconium stained amniotic fluid observed during term labour. Materials and Methods: We conducted a prospective cohort study enrolling all consenting pregnant women with term singleton fetus in cephalic presentation admitted for labour with ruptured fetal membranes in the maternity units of the Yaoundé Central Hospital (YCH) and the Yaoundé Gynaeco-Obstetric and Pediatric Hospital (YGOPH) of Cameroon between December 2014 and April 2015. The exposed grouped was considered as participants having MSAF, while the non-exposed group comprised those with clear amniotic fluid (CAF). The two groups were monitored during labor using the WHO partograph, and then followed up till 72 hours after delivery. Variables studied included the colour and texture of amniotic fluid as well as maternal and fetal complications. Data was analyzed using Epi-info version 3.5.4. The chi-square and Fischer’s exact tests were appropriately used to compare the two groups. A p-value less than 5% was considered statistically significant. Results: 2376 vaginal deliveries were recorded during the study period among which MSAF was observed in 265 cases, hence a prevalence rate of MSAF of 11.15%. Among these cases of MSAF, 52.1% was thick meconium and 47.9% was light meconium. Maternal morbidity was high in the group with MSAF;these included: Higher proportions of caesarean delivery (RR = 2.35 p -4) and prolonged labor (RR = 3 p -4). In this same group, the incidences of chorioamnionitis and puerperal sepsis were low (0.94% and 0.70% respectively), although there was a three-fold higher risk that was not statistically significant (RR = 3, P = 0.31). Fetal and neonatal outcomes were poorer in the MSAF group compared to the CAF group. The complications included fetal heart rate abnormalities, low Apgar score at the 5th minute, need for neonatal resuscitation, neonatal asphyxia and neonatal infection which were significantly higher in the MSAF group (all p < 0.05). Meconium aspiration syndrome (MAS) was found in 2.34% of MSAF cases. Perinatal mortality was 2.34% and all cases of death occurred in the thick MSAF group. Conclusion: MSAF observed during labour is associated with increased perinatal morbidity and mortality. Its detection during labor should strongly indicate very rigorous intra partum and postpartum monitoring. This will ensure optimal management and reduction in the risks of complications.展开更多
We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). XPS analysis showed that after the cane was stained by Lasiodiplodia theobrom...We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). XPS analysis showed that after the cane was stained by Lasiodiplodia theobromae, carbon and oxygen elements and the ratio of oxygen to carbon decreased. Considering atomic binding,C_1 and C_4 contents increased, while C_2 and C_3 contents decreased, and the ratio of O_2 to O_1 decreased sharply. The relative contents of lignin, cellulose and polysaccharides increased and new substances with low O_2/O_1 ratio occurred. FTIR analysis showed that the absorption peaks of O–H at 3346 cm^(-1), aliphatic C–H at 2921, 2853 and1464 cm^(-1), and C=O at 1723 cm^(-1), were characteristic peaks of fungal melanin intensified, indicating that cane discoloration was primarily caused by fungal melanin. The absorption peaks characterizing cellulose and lignin like polysaccharides at 800 cm^(-1), C–H at 1374 cm^(-1), C–O at1058 and 1038 cm^(-1), phenolic hydroxyl at 1245 cm^(-1),aromatic ether bonds at 1270 cm^(-1), carbon skeleton at1608 cm^(-1) and benzene ring at 1500 cm^(-1) were enhanced since the fungus mainly consumed the extractives in cane cell lumens and the main composition content increased relatively. Regardless of the discoloration caused by natural fungi or inoculated fungi, the discoloring feature and composition changes were identical except that the fungusinoculated cane had more melanin.展开更多
Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion model.Given that mic...Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion model.Given that microglia exhibit different morphologies in different regions after ischemic stroke,we demonstrate the superiority and necessity of using TTC-stained brain tissue to analyze the expression of various proteins or genes in different regions based on microglia character.Methods:We compared brain tissue(left for 10 min on ice)from the improved TTC staining method with penumbra from the traditional sampling method.We identified the feasibility and necessity of the improved staining method using real time(RT)-PCR,Western blot,and immunofluorescence analysis.Results:There was no protein and RNA degradation in the TTC-stained brain tissue group.However,the TREM2 specifically expressed on the microglia showed a significant difference between two groups in the penumbra region.Conclusions:TTC-stained brain tissue can be used for molecular biology experiments without any restrictions.In addition,TTC-stained brain tissue shows greater superiority due to its precise positioning.展开更多
[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and obs...[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and observed under microscope. Taenia solium specimens with clear structure and bright color were made. [ Results] Male reproductive system and excretory system of Taenia solium segment could be observed under the optical microscope, structures of uterus and eggs in Taenia solium gravid proglottid could also be observed. [ Conclusion] Aiming at providing a reference for the identification of Taenia solium and the diagnosis of the tapeworm disease.展开更多
Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and e...Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and eosin(H&E)stained tissues with high space-bandwidth and reduced artifacts.However,current FPM implementations require either precise system calibration and high-quality raw data,or significant computational loads due to iterative algorithms,which limits the practicality of FPM in routine pathological examinations.In this work,latent wavefront denoting the unobservable exiting wave at the surface of the sensor is introduced.A latent wavefront physical model optimized with variational expectation maximization(VEM)is proposed to tackle the inverse problem of FPM.The VEM-FPM alternates between solving a non-convex optimization problem as the main task for the latent wavefront in the spatial domain and merging together their Fourier spectrum in the Fourier plane as an intermediate product by solving a convex closed-formed Fourier space optimization.The VEM-FPM approach enables a stitching-free,full-field reconstruction for Fourier ptychography over a 5.3 mm×5.3 mm field of view,using a 2.5×objective with a numerical aperture(NA)of 0.08.The synthetic aperture achieves a resolution equivalent to 0.53 NA at 532 nm wavelength.The execution speed of VEM-FPM is twice as fast as that of state-of-the-art feature-domain methods while maintaining comparable reconstruction quality.展开更多
The fluoroehrome DAPI(4’-6-diamidino-2-phenylindole), possessing binding properties with adenine-thymine-rich DNA of various origins, characterized by its high specificity, sensitivity and simplicity has been used fo...The fluoroehrome DAPI(4’-6-diamidino-2-phenylindole), possessing binding properties with adenine-thymine-rich DNA of various origins, characterized by its high specificity, sensitivity and simplicity has been used for the in situ studies of both cellular and extra-cellular DNA in various prokaryotie and enkaryotic organisms, but it has not yet been used in blue green algae. This note reports the observations on the variations of DNA in the differentiated cells of Anabaena azollae by means of DAPI staining.展开更多
Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydro...Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydrological processes;however, there are few studies regarding their effects on soil preferential flow in the Mu Us sandy land. An evaluation of the effects of zokor disturbance on their habitat and soil water is important for understanding the ecological role of zokors in the soil ecosystem of the Mu Us sandy land. A field dye-tracing experiment was conducted in the Gechougou watershed on the southeastern edge of the Mu Us sandy land to investigate the effect of zokor burrowing activity on soil preferential flow characteristics. Our results showed that the density of zokor tunnels was the highest(0.40–0.46 m m^(-2)) under 30%–50% vegetation coverage and that the tunnels were approximately 3 cm from the surface.Both stained area ratio and stained path number were higher at sites with zokors than without zokors. Stained path widths were 10–80 and > 80 mm at zokor-harboring sites exhibiting homogeneous flow and heterogeneous finger flow, respectively. In the absence of zokors, homogeneous flow and highly interacted macropore flow were predominant. Soil water content below the zokor tunnels was higher than that above the tunnels. Moderate disturbance of soil structure by zokor activity facilitated soil water infiltration. These results enabled a better understanding of the effect of soil fauna on soil structure and hydrological processes and provided recommendations for ecological construction and renovation in arid and semi-arid regions.展开更多
Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific stai...Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific staining procedures involving carcinogenic chemicals.This study proposes an interdisciplinary approach merging materials science,medicine,and artificial intelligence(AI)to develop a virtual staining technique and intelligent evaluation model based on deep learning for chronic wound tissue pathology.This innovation aims to enhance clinical diagnosis and treatment by offering personalized AI-driven therapeutic strategies.By establishing a mouse model of chronic wounds and using a series of hydrogel wound dressings,tissue pathology sections were periodically collected for manual staining and healing assessment.We focused on leveraging the pix2pix image translation framework within deep learning networks.Through CNN models implemented in Python using PyTorch,our study involves learning and feature extraction for region segmentation of pathological slides.Comparative analysis between virtual staining and manual staining results,along with healing diagnosis conclusions,aims to optimize AI models.Ultimately,this approach integrates new metrics such as image recognition,quantitative analysis,and digital diagnostics to formulate an intelligent wound assessment model,facilitating smart monitoring and personalized treatment of wounds.In blind evaluation by pathologists,minimal disparities were found between virtual and conventional histologically stained images of murine wound tissue.The evaluation used pathologists’average scores on real stained images as a benchmark.The scores for virtual stained images were 71.1%for cellular features,75.4%for tissue structures,and 77.8%for overall assessment.Metrics such as PSNR(20.265)and SSIM(0.634)demonstrated our algorithms’superior performance over existing networks.Eight pathological features such as epidermis,hair follicles,and granulation tissue can be accurately identified,and the images were found to be more faithful to the actual tissue feature distribution when compared to manually annotated data.展开更多
The abundance of live and dead Oithona similis in Jiaozhou Bay,Shandong,China,was investigated monthly from December 2008 to November 2009 via the neutral red staining method.The annual variation in total abundance wa...The abundance of live and dead Oithona similis in Jiaozhou Bay,Shandong,China,was investigated monthly from December 2008 to November 2009 via the neutral red staining method.The annual variation in total abundance was similar for females,males,and copepodites,with a main numerical peak from February to July and a minor peak in November,but the variations in carcass abundance and death proportion were different.The monthly average abundance of male and copepodite carcasses significantly increased with their total abundance(P<0.001),whereas no such correlation was observed for females.The abundance of female carcasses peaked at 12.4 inds./m^(3) in November but remained below 3.0 inds./m^(3) in all other months.The monthly average death proportion ranged 0.3%-4.9% for females,3.6%-25.7% for males,and 2.6%-8.4% for copepodites.On average,the death proportion of males was 9.2 times greater than that of females and 3.5 times greater than that of copepodites.Therefore,significant sexual difference exists in the non-predatory mortality of O.similis and this difference is an important cause of the female-skewed sex ratios observed in the field.展开更多
Compared to refined wheat flour,whole wheat flour contains higher nutrients,but its high content of dietary fiber can have a significant impact on the quality of the final product.Therefore,how to enable consumers to ...Compared to refined wheat flour,whole wheat flour contains higher nutrients,but its high content of dietary fiber can have a significant impact on the quality of the final product.Therefore,how to enable consumers to obtain health benefits from whole wheat flour and improve the processing performance of whole wheat products has become a concern.The purpose of this study is to apply 2 strains Bacillus sp.SH and Aspergillus oryzae Y21 with cellulase production capacity and study their adaptability in whole wheat dough.The results indicate that the addition of cellulase-producing strains enhanced the acid production ability of whole wheat dough,rapidly reduced its pH value and insoluble dietary fiber content,and significantly increased the water-soluble arabinoxylan and water-soluble dietary fiber content.During the fermentation process,the viscoelasticity of the dough decreased,free sulfhydryl content increased,wet gluten content decreased,and the degree of reduction was consistent with the degree of acidification.Moreover,the proteolytic activity of the dough was increased,and the hydrolysis of gliadin was the most extensive.SH showed a higher advantage and has been used in whole wheat bread making.Increasing the proportion of strain SH in whole wheat bread can improve the structural characteristics and texture of the bread.When SH(5×10~7 CFU/g)is added to whole wheat bread,its hardness,elasticity,chewiness,and resilience can be similar to those of bread made from control group wheat flour,far exceeding that of whole wheat bread without adding SH.The addition of cellulase producing strains has obvious advantages in the development of whole-wheat dough,and also promote the development of whole wheat fermented foods as staple foods.展开更多
With the deepening of epigenetic research,studies have shown that N6-methyladenosine(m^(6)A)is closely related to the development of rheumatoid arthritis(RA),but the mechanism is still unclear.In the study,we collecte...With the deepening of epigenetic research,studies have shown that N6-methyladenosine(m^(6)A)is closely related to the development of rheumatoid arthritis(RA),but the mechanism is still unclear.In the study,we collected synovial tissues from normal controls and patients with osteoarthritis(OA)or RA.The levels of m^(6)A and inflammation were analyzed by immunofluorescence staining and western blotting.The roles of IGF2BP3 in cell proliferation and inflammatory activation were explored using transfection and RNA immunoprecipitation assays.IGF2BP3^(−/−)mice were generated and used to establish an arthritis mouse model by transferring serum from adult arthritis K/BxN mice.We found m^(6)A levels were markedly increased in RA patients and mouse models,and the expression of IGF2BP3 was upregulated in individuals with RA and related to the levels of inflammatory markers.IGF2BP3 played an important part in RA-fibroblast-like synoviocytes(FLS)by promoting cell proliferation,migration,invasion,inflammatory cytokine release and inhibiting autophagy.In addition,IGF2BP3 inhibited autophagy to reduce ROS production,thereby decreasing the inflammatory activation of macrophages.More importantly,RASGRF1-mediated mTORC1 activation played a crucial role in the ability of IGF2BP3 to promote cell proliferation and inflammatory activation.In an arthritis model of IGF2BP3^(−/−)mice,IGF2BP3 knockout inhibited RA-FLS proliferation and inflammatory infiltration,and further ameliorated RA joint injury.Our study revealed an important role for IGF2BP3 in RA progression.The targeted inhibition of IGF2BP3 reduced cell proliferation and inflammatory activation and limited RA development,providing a potential strategy for RA therapy.展开更多
Background: Diarrheal diseases have globally decreased over the past few decades, yet they remain one of the top three causes of mortality in children under five years, especially in sub-Saharan Africa and Nigeria. Se...Background: Diarrheal diseases have globally decreased over the past few decades, yet they remain one of the top three causes of mortality in children under five years, especially in sub-Saharan Africa and Nigeria. Seasonal peaks of diarrheal episodes continue to contribute significantly to childhood mortality in these regions. One of the notable causes of diarrhea in children is parasitic infections, particularly Cryptosporidium, which poses a serious health risk. In Nigeria, the burden of Cryptosporidium diarrhea is under-researched, making it imperative to investigate its prevalence and associated risk factors. Study Objectives: The study aims to determine the prevalence and risk factors associated with Cryptosporidium diarrhea among children aged five years and below in selected health institutions in Abakaliki, South-East Nigeria. Methodology: This cross-sectional study was conducted from January to May 2017, recruiting 200 children under five years with diarrhea from health institutions in Abakaliki. Fecal specimens were analyzed for Cryptosporidium oocysts using light microscopy with modified Ziehl-Neelsen staining and immunofluorescent antibody test (IFAT). Deoxyribonucleic acid (DNA) was extracted from positive samples using QIAmp® DNA stool kit, followed by Polymerase Chain Reaction (PCR) and molecular genotyping. Results: Cryptosporidium was detected in 0.5% (1/200) of children via light microscopy and 6.5% (13/200) via IFAT. All positive samples were confirmed as Cryptosporidium hominis by PCR. The prevalence of infection was significantly higher in children from institutionalized homes (50.0%) compared to monogamous homes (6.2%) (p Conclusion: Cryptosporidium hominis is a notable cause of diarrhea among children in Abakaliki, primarily transmitted through human-to-human contact. The study underscores the need for targeted interventions in childcare institutions to prevent outbreaks. Health authorities should promote breastfeeding and enhance education on hygiene practices in vulnerable populations.展开更多
This study investigates the variability in cancer diagnosis across different tissues and organs, with a focus on the role of diagnostic methods such as Hematoxylin and Eosin (H&E) staining and immunohistochemistry...This study investigates the variability in cancer diagnosis across different tissues and organs, with a focus on the role of diagnostic methods such as Hematoxylin and Eosin (H&E) staining and immunohistochemistry (IHC). The predominance of female breast cancer (30%) aligns with global trends, underscoring the need for robust diagnostic protocols, particularly in developing regions. Other prevalent cancers, including skin, stomach, and cervix uteri, reflect a mix of environmental, genetic, and infectious factors. The underrepresentation of gallbladder and thyroid cancers (<1%) suggests potential underdiagnosis or lower prevalence. Age distribution data indicate peak cancer incidence in individuals aged 31 - 45 years, with gender-specific cancers like breast and cervical cancer predominantly affecting females (63.4%). The analysis also highlights significant diagnostic gaps, as 61.2% of cases did not undergo IHC testing due to resource constraints, leading to potential biases in cancer prevalence and diagnostic accuracy. The study emphasizes the complementary role of IHC in confirming ambiguous H&E findings, with strong alignment observed when both methods were used. However, the absence of IHC in many cases limits the robustness of conclusions, suggesting the need for increased access to IHC testing. The findings advocate for integrating IHC into routine diagnostics, expanding diagnostic capabilities, and improving sample sizes to ensure more reliable and comprehensive cancer data.展开更多
Objective: To characterize the expression of ST13 protein in human tissuesfor investigation of the function of colorectal cancer related gene ST13. Methods: ST13 ORF wascloned and over-expressed in E.coli. The recombi...Objective: To characterize the expression of ST13 protein in human tissuesfor investigation of the function of colorectal cancer related gene ST13. Methods: ST13 ORF wascloned and over-expressed in E.coli. The recombinant ST13 protein was purified by affinitychromatography. ST13 monoclonal antibodies were generated and affinity purified with the recombinantprotein. Immunoblot and immunohistochemical staining were employed to analyze ST13 proteinexpression in human tissues. Results: The expression and purification of the recombinant ST13protein were confirmed by SDS-PAGE. The protein yield reached about 2.5 mg/L of induced bacterialculture with a purity of 91.3%. Three strains of hybridoma were obtained with antibody titers from10~4 to 10~5 in ascites fluids and with high specificity for ST13 protein. Immunoblot showed thatthe apparent Mr of ST13 protein in SW480 cells and human tissues estimated by SDS-PAGE mobility wasapproximately 50 000, which was about 10 000 larger than the 41 324 calculated, but theglycosylation of the protein was excluded. Computer modeling revealed the protein to be ahydrophilic molecule. Immunohistochemical staining showed that ST13 protein was evenly distributedin cytoplasm and expressed in colon, stomach, liver, and other epithelial cells. Differences in thestaining intensity of the protein were observed between normal and cancer tissues as well as amongdifferent normal or carcinoma tissues. Conclusion: ST13 protein is a cytoplasmic molecule with anapparent Mr of 50 000. The protein is expressed in colorectal and other epithelial tissues. Theexpression level of the protein is down-regulated in colorectal cancer and varies among differentnormal and/or carcinoma tissues. Comparison of cDNA sequences and protein characteristics indicatesthat ST13 protein and hsp70-interacting protein (Hip) are same proteins, raising the possibilitythat ST13 protein is involved in the development of colorectal cancer through Hsp70 molecularchaperone machinery.展开更多
Objective To investigate whether hypertonic saline （HS） can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and...Objective To investigate whether hypertonic saline （HS） can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and identified from the hypothalamus of newborn rat （1 day）. The astrocytes were randomly divided into five groups： isotonic （IS） and HS groups, astrocytes were incubated by IS and HS （320 mosM NaCl） medium, respectively, for 1, 3, 5, 10 or 15 rain; carbenoxolone （CBX） ＋IS and CBX＋HS groups, astrocytes were pre-treated with CBX （100 mmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, then removed to IS and HS medium, respectively, for 1, 3, 5, 10 or 15 min; Ca2＋＋HS group, astrocytes were pre-incubated with Ca2＋ （1 000 μmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, followed by a wash with isotonic FBS/DMEM, and then removed to hypertonic saline for 1, 3, 5, 10 or 15 min. The media of five groups were collected to analyze the medium glutamate concentration with high performance liquid chromatography. The astrocytes were fixed and double immunofluorescent stained with anti-glial fibrillary acidic protein （GFAP） and anti-glutamate. The C6 cells were divided into four groups： IS, HS, CBX＋IS and CBX＋HS groups, and used for quantitative measurement of glutamate in cells by flow cytometry （FCM）. Results （1） Anti-GFAP immunofluorescent signal revealed no significant difference among various time points in each group, or among the five groups. （2） The anti-glutamate immunofluorescent signal was increased in HS group and peaked at 5 min, and decreased and returned to the level of IS group at 15 rain （P 〈 0.01 vs the 5 min of HS group）. In CBX＋HS group, the glutamate intensity was higher than that in CBX＋IS and HS groups. （3） The medium glutamate concentration had no change after treatment with HS for 1 and 3 min, while increased markedly after treatment for 5 min to 15 min （P 〈 0.01 vs 1 min and 3 min）. On the contrary, the medium glutamate concentrations in the CBX＋HS or Ca2＋＋HS group were significant lower than that in the HS group （P 〈 0.01）. （4） FCM showed HS and CBX＋HS induced glutamate increase in C6 cells. Conclusion HS induced cultured rat hypothalamic astrocytes or C6 cells to synthesize and release glutamate; CBX could block glutamate release, but could not disrupt glutamate synthesis.展开更多
基金Shenzhen Key Fundamental Research Project(No.JCYJ20210324120012035).
文摘Recently,Mueller matrix(MM)polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis.However,since our human eyes cannot observe polarized light directly,it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images.One feasible approach is to combine MM polarimetric imaging with virtual staining techniques to generate standardized stained images,inheriting the advantages of information-abundant MM polarimetric imaging.In this study,we develop a model using unpaired MM polarimetric images and bright-field images for generating standard hematoxylin and eosin(H&E)stained tissue images.Compared with the existing polarization virtual staining techniques primarily based on the model training with paired images,the proposed Cycle-Consistent Generative Adversarial Networks(CycleGAN)-based model simplifies data acquisition and data preprocessing to a great extent.The outcomes demonstrate the feasibility of training CycleGAN with unpaired polarization images and their corresponding bright-field images as a viable approach,which provides an intuitive manner for pathologists for future polarization-assisted digital pathology.
基金National Natural Science Foundation of China(82274411)Science and Technology Innovation Program of Hunan Province(2022RC1021)Leading Research Project of Hunan University of Chinese Medicine(2022XJJB002).
文摘Objective To build a dataset encompassing a large number of stained tongue coating images and process it using deep learning to automatically recognize stained tongue coating images.Methods A total of 1001 images of stained tongue coating from healthy students at Hunan University of Chinese Medicine and 1007 images of pathological(non-stained)tongue coat-ing from hospitalized patients at The First Hospital of Hunan University of Chinese Medicine withlungcancer;diabetes;andhypertensionwerecollected.Thetongueimageswererandomi-zed into the training;validation;and testing datasets in a 7:2:1 ratio.A deep learning model was constructed using the ResNet50 for recognizing stained tongue coating in the training and validation datasets.The training period was 90 epochs.The model’s performance was evaluated by its accuracy;loss curve;recall;F1 score;confusion matrix;receiver operating characteristic(ROC)curve;and precision-recall(PR)curve in the tasks of predicting stained tongue coating images in the testing dataset.The accuracy of the deep learning model was compared with that of attending physicians of traditional Chinese medicine(TCM).Results The training results showed that after 90 epochs;the model presented an excellent classification performance.The loss curve and accuracy were stable;showing no signs of overfitting.The model achieved an accuracy;recall;and F1 score of 92%;91%;and 92%;re-spectively.The confusion matrix revealed an accuracy of 92%for the model and 69%for TCM practitioners.The areas under the ROC and PR curves were 0.97 and 0.95;respectively.Conclusion The deep learning model constructed using ResNet50 can effectively recognize stained coating images with greater accuracy than visual inspection of TCM practitioners.This model has the potential to assist doctors in identifying false tongue coating and prevent-ing misdiagnosis.
文摘An innovative, cost-effective, user-friendly and portable device （dew sensor） to directly detect condensation on glass surfaces was developed within the EC-VIDRIO project （contract No. EVK4-CT-2001-00045）, aimed at finding sustainable solutions to preserve ancient stained glass windows. The results of the research showed that the direct survey of condensation with the new sensor is more accurate and reliable than the traditional indirect microclimatic measurements. Since the construction of the first prototype, the dew sensor was developed further, continuously improved, validated in the laboratory and applied successfully on different surfaces at sites of interest in the field of Cultural Heritage; on the stained glass windows in the Saint Urbain Basilica of Troyes （France）, Sainte Chapelle of Paris （France） and Cologne Cathedral （Germany）; on stone walls in Petrarca＇s Tomb （Padua, Italy） and the Hagar Qim Temple （Malta）. Now the sensor is being used in the Lascaux Caves （France）. The newly built device was patented （PTC/EP2005/050665） and gave such interesting results that the Italian Ministry of University and Research financially supported a spin-off project that has lead to the creation of a new company （R.E.D.s.r.l.） in order to develop the prototype further and to produce the sensor at industrial scale.
文摘Background: Meconium stained amniotic fluid (MSAF) is frequently encountered in obstetric practice. Literature on the subject is still poorly documented in the African setting. Objective: The aim of this study was to determine the maternal and fetal outcomes in case of meconium stained amniotic fluid observed during term labour. Materials and Methods: We conducted a prospective cohort study enrolling all consenting pregnant women with term singleton fetus in cephalic presentation admitted for labour with ruptured fetal membranes in the maternity units of the Yaoundé Central Hospital (YCH) and the Yaoundé Gynaeco-Obstetric and Pediatric Hospital (YGOPH) of Cameroon between December 2014 and April 2015. The exposed grouped was considered as participants having MSAF, while the non-exposed group comprised those with clear amniotic fluid (CAF). The two groups were monitored during labor using the WHO partograph, and then followed up till 72 hours after delivery. Variables studied included the colour and texture of amniotic fluid as well as maternal and fetal complications. Data was analyzed using Epi-info version 3.5.4. The chi-square and Fischer’s exact tests were appropriately used to compare the two groups. A p-value less than 5% was considered statistically significant. Results: 2376 vaginal deliveries were recorded during the study period among which MSAF was observed in 265 cases, hence a prevalence rate of MSAF of 11.15%. Among these cases of MSAF, 52.1% was thick meconium and 47.9% was light meconium. Maternal morbidity was high in the group with MSAF;these included: Higher proportions of caesarean delivery (RR = 2.35 p -4) and prolonged labor (RR = 3 p -4). In this same group, the incidences of chorioamnionitis and puerperal sepsis were low (0.94% and 0.70% respectively), although there was a three-fold higher risk that was not statistically significant (RR = 3, P = 0.31). Fetal and neonatal outcomes were poorer in the MSAF group compared to the CAF group. The complications included fetal heart rate abnormalities, low Apgar score at the 5th minute, need for neonatal resuscitation, neonatal asphyxia and neonatal infection which were significantly higher in the MSAF group (all p < 0.05). Meconium aspiration syndrome (MAS) was found in 2.34% of MSAF cases. Perinatal mortality was 2.34% and all cases of death occurred in the thick MSAF group. Conclusion: MSAF observed during labour is associated with increased perinatal morbidity and mortality. Its detection during labor should strongly indicate very rigorous intra partum and postpartum monitoring. This will ensure optimal management and reduction in the risks of complications.
基金supported by the National Key Project of S&T Supporting Programs Funded by MOST of China during the 12th Five-year Plan(No.2012BAD23B0104)
文摘We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FTIR). XPS analysis showed that after the cane was stained by Lasiodiplodia theobromae, carbon and oxygen elements and the ratio of oxygen to carbon decreased. Considering atomic binding,C_1 and C_4 contents increased, while C_2 and C_3 contents decreased, and the ratio of O_2 to O_1 decreased sharply. The relative contents of lignin, cellulose and polysaccharides increased and new substances with low O_2/O_1 ratio occurred. FTIR analysis showed that the absorption peaks of O–H at 3346 cm^(-1), aliphatic C–H at 2921, 2853 and1464 cm^(-1), and C=O at 1723 cm^(-1), were characteristic peaks of fungal melanin intensified, indicating that cane discoloration was primarily caused by fungal melanin. The absorption peaks characterizing cellulose and lignin like polysaccharides at 800 cm^(-1), C–H at 1374 cm^(-1), C–O at1058 and 1038 cm^(-1), phenolic hydroxyl at 1245 cm^(-1),aromatic ether bonds at 1270 cm^(-1), carbon skeleton at1608 cm^(-1) and benzene ring at 1500 cm^(-1) were enhanced since the fungus mainly consumed the extractives in cane cell lumens and the main composition content increased relatively. Regardless of the discoloration caused by natural fungi or inoculated fungi, the discoloring feature and composition changes were identical except that the fungusinoculated cane had more melanin.
基金National Natural Science Foundation of China,Grant/Award Number:81730031 to lili and 82001395 to yingwei wangthe Foundation of Shanghai Municipal Key Clinical Specialty,Grant/Award Number:shslczdzk06901 to yingwei wang。
文摘Background:TTC(2,3,5-triphenyltetrazolium chloride)staining is the most commonly used method in identifying and assessing cerebral infarct volumes in the transient middle cerebral artery occlusion model.Given that microglia exhibit different morphologies in different regions after ischemic stroke,we demonstrate the superiority and necessity of using TTC-stained brain tissue to analyze the expression of various proteins or genes in different regions based on microglia character.Methods:We compared brain tissue(left for 10 min on ice)from the improved TTC staining method with penumbra from the traditional sampling method.We identified the feasibility and necessity of the improved staining method using real time(RT)-PCR,Western blot,and immunofluorescence analysis.Results:There was no protein and RNA degradation in the TTC-stained brain tissue group.However,the TREM2 specifically expressed on the microglia showed a significant difference between two groups in the penumbra region.Conclusions:TTC-stained brain tissue can be used for molecular biology experiments without any restrictions.In addition,TTC-stained brain tissue shows greater superiority due to its precise positioning.
文摘[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin （HE） staining method and observed under microscope. Taenia solium specimens with clear structure and bright color were made. [ Results] Male reproductive system and excretory system of Taenia solium segment could be observed under the optical microscope, structures of uterus and eggs in Taenia solium gravid proglottid could also be observed. [ Conclusion] Aiming at providing a reference for the identification of Taenia solium and the diagnosis of the tapeworm disease.
基金National Natural Science Foundation of China(62235009)。
文摘Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and eosin(H&E)stained tissues with high space-bandwidth and reduced artifacts.However,current FPM implementations require either precise system calibration and high-quality raw data,or significant computational loads due to iterative algorithms,which limits the practicality of FPM in routine pathological examinations.In this work,latent wavefront denoting the unobservable exiting wave at the surface of the sensor is introduced.A latent wavefront physical model optimized with variational expectation maximization(VEM)is proposed to tackle the inverse problem of FPM.The VEM-FPM alternates between solving a non-convex optimization problem as the main task for the latent wavefront in the spatial domain and merging together their Fourier spectrum in the Fourier plane as an intermediate product by solving a convex closed-formed Fourier space optimization.The VEM-FPM approach enables a stitching-free,full-field reconstruction for Fourier ptychography over a 5.3 mm×5.3 mm field of view,using a 2.5×objective with a numerical aperture(NA)of 0.08.The synthetic aperture achieves a resolution equivalent to 0.53 NA at 532 nm wavelength.The execution speed of VEM-FPM is twice as fast as that of state-of-the-art feature-domain methods while maintaining comparable reconstruction quality.
文摘The fluoroehrome DAPI(4’-6-diamidino-2-phenylindole), possessing binding properties with adenine-thymine-rich DNA of various origins, characterized by its high specificity, sensitivity and simplicity has been used for the in situ studies of both cellular and extra-cellular DNA in various prokaryotie and enkaryotic organisms, but it has not yet been used in blue green algae. This note reports the observations on the variations of DNA in the differentiated cells of Anabaena azollae by means of DAPI staining.
基金supported by the National Natural Science Foundation of China(Nos.41807011 and 41907012)the Chinese Universities Scientific Fund(No.2452022335)the Open Fund for Key Lab.of Ecological Study of Ningxia University,China(No.LDER2022Z01)。
文摘Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydrological processes;however, there are few studies regarding their effects on soil preferential flow in the Mu Us sandy land. An evaluation of the effects of zokor disturbance on their habitat and soil water is important for understanding the ecological role of zokors in the soil ecosystem of the Mu Us sandy land. A field dye-tracing experiment was conducted in the Gechougou watershed on the southeastern edge of the Mu Us sandy land to investigate the effect of zokor burrowing activity on soil preferential flow characteristics. Our results showed that the density of zokor tunnels was the highest(0.40–0.46 m m^(-2)) under 30%–50% vegetation coverage and that the tunnels were approximately 3 cm from the surface.Both stained area ratio and stained path number were higher at sites with zokors than without zokors. Stained path widths were 10–80 and > 80 mm at zokor-harboring sites exhibiting homogeneous flow and heterogeneous finger flow, respectively. In the absence of zokors, homogeneous flow and highly interacted macropore flow were predominant. Soil water content below the zokor tunnels was higher than that above the tunnels. Moderate disturbance of soil structure by zokor activity facilitated soil water infiltration. These results enabled a better understanding of the effect of soil fauna on soil structure and hydrological processes and provided recommendations for ecological construction and renovation in arid and semi-arid regions.
基金supported by the Fundamental Research Funds for the Central Universities(No.20720230037)the National Natural Science Foundation of China(No.52273305)+2 种基金Natural Science Foundation of Fujian Province of China(No.2023J05012)State Key Laboratory of Vaccines for Infectious Diseases,Xiang An Biomedicine Laboratory(Nos.2023XAKJ0103071,2023XAKJ0102061)Natural Science Foundation of Xiamen,China(No.3502Z20227010).
文摘Histopathological analysis of chronic wounds is crucial for clinicians to accurately assess wound healing progress and detect potential malignancy.However,traditional pathological tissue sections require specific staining procedures involving carcinogenic chemicals.This study proposes an interdisciplinary approach merging materials science,medicine,and artificial intelligence(AI)to develop a virtual staining technique and intelligent evaluation model based on deep learning for chronic wound tissue pathology.This innovation aims to enhance clinical diagnosis and treatment by offering personalized AI-driven therapeutic strategies.By establishing a mouse model of chronic wounds and using a series of hydrogel wound dressings,tissue pathology sections were periodically collected for manual staining and healing assessment.We focused on leveraging the pix2pix image translation framework within deep learning networks.Through CNN models implemented in Python using PyTorch,our study involves learning and feature extraction for region segmentation of pathological slides.Comparative analysis between virtual staining and manual staining results,along with healing diagnosis conclusions,aims to optimize AI models.Ultimately,this approach integrates new metrics such as image recognition,quantitative analysis,and digital diagnostics to formulate an intelligent wound assessment model,facilitating smart monitoring and personalized treatment of wounds.In blind evaluation by pathologists,minimal disparities were found between virtual and conventional histologically stained images of murine wound tissue.The evaluation used pathologists’average scores on real stained images as a benchmark.The scores for virtual stained images were 71.1%for cellular features,75.4%for tissue structures,and 77.8%for overall assessment.Metrics such as PSNR(20.265)and SSIM(0.634)demonstrated our algorithms’superior performance over existing networks.Eight pathological features such as epidermis,hair follicles,and granulation tissue can be accurately identified,and the images were found to be more faithful to the actual tissue feature distribution when compared to manually annotated data.
基金Supported by the Shandong Provincial Natural Science Foundation(No.ZR2020QC043)。
文摘The abundance of live and dead Oithona similis in Jiaozhou Bay,Shandong,China,was investigated monthly from December 2008 to November 2009 via the neutral red staining method.The annual variation in total abundance was similar for females,males,and copepodites,with a main numerical peak from February to July and a minor peak in November,but the variations in carcass abundance and death proportion were different.The monthly average abundance of male and copepodite carcasses significantly increased with their total abundance(P<0.001),whereas no such correlation was observed for females.The abundance of female carcasses peaked at 12.4 inds./m^(3) in November but remained below 3.0 inds./m^(3) in all other months.The monthly average death proportion ranged 0.3%-4.9% for females,3.6%-25.7% for males,and 2.6%-8.4% for copepodites.On average,the death proportion of males was 9.2 times greater than that of females and 3.5 times greater than that of copepodites.Therefore,significant sexual difference exists in the non-predatory mortality of O.similis and this difference is an important cause of the female-skewed sex ratios observed in the field.
基金supported by National Natural Science Foundation of China(32330081)。
文摘Compared to refined wheat flour,whole wheat flour contains higher nutrients,but its high content of dietary fiber can have a significant impact on the quality of the final product.Therefore,how to enable consumers to obtain health benefits from whole wheat flour and improve the processing performance of whole wheat products has become a concern.The purpose of this study is to apply 2 strains Bacillus sp.SH and Aspergillus oryzae Y21 with cellulase production capacity and study their adaptability in whole wheat dough.The results indicate that the addition of cellulase-producing strains enhanced the acid production ability of whole wheat dough,rapidly reduced its pH value and insoluble dietary fiber content,and significantly increased the water-soluble arabinoxylan and water-soluble dietary fiber content.During the fermentation process,the viscoelasticity of the dough decreased,free sulfhydryl content increased,wet gluten content decreased,and the degree of reduction was consistent with the degree of acidification.Moreover,the proteolytic activity of the dough was increased,and the hydrolysis of gliadin was the most extensive.SH showed a higher advantage and has been used in whole wheat bread making.Increasing the proportion of strain SH in whole wheat bread can improve the structural characteristics and texture of the bread.When SH(5×10~7 CFU/g)is added to whole wheat bread,its hardness,elasticity,chewiness,and resilience can be similar to those of bread made from control group wheat flour,far exceeding that of whole wheat bread without adding SH.The addition of cellulase producing strains has obvious advantages in the development of whole-wheat dough,and also promote the development of whole wheat fermented foods as staple foods.
基金supported by the National Natural Science Foundation of China(U22A20374,52373273)National High Level Hospital Clinical Research Funding of China-Japan Friendship Hospital(Grant number:2024-NHLHCRF-JBGSWZ-02).
文摘With the deepening of epigenetic research,studies have shown that N6-methyladenosine(m^(6)A)is closely related to the development of rheumatoid arthritis(RA),but the mechanism is still unclear.In the study,we collected synovial tissues from normal controls and patients with osteoarthritis(OA)or RA.The levels of m^(6)A and inflammation were analyzed by immunofluorescence staining and western blotting.The roles of IGF2BP3 in cell proliferation and inflammatory activation were explored using transfection and RNA immunoprecipitation assays.IGF2BP3^(−/−)mice were generated and used to establish an arthritis mouse model by transferring serum from adult arthritis K/BxN mice.We found m^(6)A levels were markedly increased in RA patients and mouse models,and the expression of IGF2BP3 was upregulated in individuals with RA and related to the levels of inflammatory markers.IGF2BP3 played an important part in RA-fibroblast-like synoviocytes(FLS)by promoting cell proliferation,migration,invasion,inflammatory cytokine release and inhibiting autophagy.In addition,IGF2BP3 inhibited autophagy to reduce ROS production,thereby decreasing the inflammatory activation of macrophages.More importantly,RASGRF1-mediated mTORC1 activation played a crucial role in the ability of IGF2BP3 to promote cell proliferation and inflammatory activation.In an arthritis model of IGF2BP3^(−/−)mice,IGF2BP3 knockout inhibited RA-FLS proliferation and inflammatory infiltration,and further ameliorated RA joint injury.Our study revealed an important role for IGF2BP3 in RA progression.The targeted inhibition of IGF2BP3 reduced cell proliferation and inflammatory activation and limited RA development,providing a potential strategy for RA therapy.
文摘Background: Diarrheal diseases have globally decreased over the past few decades, yet they remain one of the top three causes of mortality in children under five years, especially in sub-Saharan Africa and Nigeria. Seasonal peaks of diarrheal episodes continue to contribute significantly to childhood mortality in these regions. One of the notable causes of diarrhea in children is parasitic infections, particularly Cryptosporidium, which poses a serious health risk. In Nigeria, the burden of Cryptosporidium diarrhea is under-researched, making it imperative to investigate its prevalence and associated risk factors. Study Objectives: The study aims to determine the prevalence and risk factors associated with Cryptosporidium diarrhea among children aged five years and below in selected health institutions in Abakaliki, South-East Nigeria. Methodology: This cross-sectional study was conducted from January to May 2017, recruiting 200 children under five years with diarrhea from health institutions in Abakaliki. Fecal specimens were analyzed for Cryptosporidium oocysts using light microscopy with modified Ziehl-Neelsen staining and immunofluorescent antibody test (IFAT). Deoxyribonucleic acid (DNA) was extracted from positive samples using QIAmp® DNA stool kit, followed by Polymerase Chain Reaction (PCR) and molecular genotyping. Results: Cryptosporidium was detected in 0.5% (1/200) of children via light microscopy and 6.5% (13/200) via IFAT. All positive samples were confirmed as Cryptosporidium hominis by PCR. The prevalence of infection was significantly higher in children from institutionalized homes (50.0%) compared to monogamous homes (6.2%) (p Conclusion: Cryptosporidium hominis is a notable cause of diarrhea among children in Abakaliki, primarily transmitted through human-to-human contact. The study underscores the need for targeted interventions in childcare institutions to prevent outbreaks. Health authorities should promote breastfeeding and enhance education on hygiene practices in vulnerable populations.
文摘This study investigates the variability in cancer diagnosis across different tissues and organs, with a focus on the role of diagnostic methods such as Hematoxylin and Eosin (H&E) staining and immunohistochemistry (IHC). The predominance of female breast cancer (30%) aligns with global trends, underscoring the need for robust diagnostic protocols, particularly in developing regions. Other prevalent cancers, including skin, stomach, and cervix uteri, reflect a mix of environmental, genetic, and infectious factors. The underrepresentation of gallbladder and thyroid cancers (<1%) suggests potential underdiagnosis or lower prevalence. Age distribution data indicate peak cancer incidence in individuals aged 31 - 45 years, with gender-specific cancers like breast and cervical cancer predominantly affecting females (63.4%). The analysis also highlights significant diagnostic gaps, as 61.2% of cases did not undergo IHC testing due to resource constraints, leading to potential biases in cancer prevalence and diagnostic accuracy. The study emphasizes the complementary role of IHC in confirming ambiguous H&E findings, with strong alignment observed when both methods were used. However, the absence of IHC in many cases limits the robustness of conclusions, suggesting the need for increased access to IHC testing. The findings advocate for integrating IHC into routine diagnostics, expanding diagnostic capabilities, and improving sample sizes to ensure more reliable and comprehensive cancer data.
文摘Objective: To characterize the expression of ST13 protein in human tissuesfor investigation of the function of colorectal cancer related gene ST13. Methods: ST13 ORF wascloned and over-expressed in E.coli. The recombinant ST13 protein was purified by affinitychromatography. ST13 monoclonal antibodies were generated and affinity purified with the recombinantprotein. Immunoblot and immunohistochemical staining were employed to analyze ST13 proteinexpression in human tissues. Results: The expression and purification of the recombinant ST13protein were confirmed by SDS-PAGE. The protein yield reached about 2.5 mg/L of induced bacterialculture with a purity of 91.3%. Three strains of hybridoma were obtained with antibody titers from10~4 to 10~5 in ascites fluids and with high specificity for ST13 protein. Immunoblot showed thatthe apparent Mr of ST13 protein in SW480 cells and human tissues estimated by SDS-PAGE mobility wasapproximately 50 000, which was about 10 000 larger than the 41 324 calculated, but theglycosylation of the protein was excluded. Computer modeling revealed the protein to be ahydrophilic molecule. Immunohistochemical staining showed that ST13 protein was evenly distributedin cytoplasm and expressed in colon, stomach, liver, and other epithelial cells. Differences in thestaining intensity of the protein were observed between normal and cancer tissues as well as amongdifferent normal or carcinoma tissues. Conclusion: ST13 protein is a cytoplasmic molecule with anapparent Mr of 50 000. The protein is expressed in colorectal and other epithelial tissues. Theexpression level of the protein is down-regulated in colorectal cancer and varies among differentnormal and/or carcinoma tissues. Comparison of cDNA sequences and protein characteristics indicatesthat ST13 protein and hsp70-interacting protein (Hip) are same proteins, raising the possibilitythat ST13 protein is involved in the development of colorectal cancer through Hsp70 molecularchaperone machinery.
基金supported by the National Nature Science Foundation of China(No.39770251)the Medical Foundation of the People's Liberation Army,China(No.01Z082,06MA234)the Foundation of the Fourth Military Medical University(No.05ZXJM001)
文摘Objective To investigate whether hypertonic saline （HS） can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and identified from the hypothalamus of newborn rat （1 day）. The astrocytes were randomly divided into five groups： isotonic （IS） and HS groups, astrocytes were incubated by IS and HS （320 mosM NaCl） medium, respectively, for 1, 3, 5, 10 or 15 rain; carbenoxolone （CBX） ＋IS and CBX＋HS groups, astrocytes were pre-treated with CBX （100 mmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, then removed to IS and HS medium, respectively, for 1, 3, 5, 10 or 15 min; Ca2＋＋HS group, astrocytes were pre-incubated with Ca2＋ （1 000 μmol/L） for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, followed by a wash with isotonic FBS/DMEM, and then removed to hypertonic saline for 1, 3, 5, 10 or 15 min. The media of five groups were collected to analyze the medium glutamate concentration with high performance liquid chromatography. The astrocytes were fixed and double immunofluorescent stained with anti-glial fibrillary acidic protein （GFAP） and anti-glutamate. The C6 cells were divided into four groups： IS, HS, CBX＋IS and CBX＋HS groups, and used for quantitative measurement of glutamate in cells by flow cytometry （FCM）. Results （1） Anti-GFAP immunofluorescent signal revealed no significant difference among various time points in each group, or among the five groups. （2） The anti-glutamate immunofluorescent signal was increased in HS group and peaked at 5 min, and decreased and returned to the level of IS group at 15 rain （P 〈 0.01 vs the 5 min of HS group）. In CBX＋HS group, the glutamate intensity was higher than that in CBX＋IS and HS groups. （3） The medium glutamate concentration had no change after treatment with HS for 1 and 3 min, while increased markedly after treatment for 5 min to 15 min （P 〈 0.01 vs 1 min and 3 min）. On the contrary, the medium glutamate concentrations in the CBX＋HS or Ca2＋＋HS group were significant lower than that in the HS group （P 〈 0.01）. （4） FCM showed HS and CBX＋HS induced glutamate increase in C6 cells. Conclusion HS induced cultured rat hypothalamic astrocytes or C6 cells to synthesize and release glutamate; CBX could block glutamate release, but could not disrupt glutamate synthesis.
