In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes control...In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes controlling the groundwater chemistry is a major step in the sustainable management of the aquifers.A total of 29groundwater samples were collected and analysed.Ionic ratio graphs,multivariate statistical analysis,mineral saturation indices,stable isotopes,and geostatistics methods were used to examine the sources and the quality of the groundwater.The findings describe the water types in the district as Ca-Mg-HCO_(3)-Cl,Ca-Na-HCO_(3),Na-Ca-HCO_(3),Ca-Na-HCO_(3)-Cl,Na-Ca-HCO_(3)-Cl,mix water type,NaHCO_(3)-Cl,with possible evolution to Ca-Na-Cl-HCO_(3),and Na-Ca-Cl-HCO_(3).According to the IEWQI for drinking water,around 53.6% of the samples have good quality,whereas 10.7% have very low-quality groundwater.Only 3.45% of the samples are suitable to use for irrigation without treatment,whereas 41.4% are somewhat safe with minimal treatment.Water-rock interactions,including the dissolution and weathering of silicate minerals,cation exchange processes,and human activities like mining andquarrying,are some of the main factors influencing groundwater chemistry.Principal component analysis revealed that groundwater chemistry is influenced by a combination of natural and anthropogenic sources.The APCs-MLR receptor model quantifies the factors that play important roles in groundwater salinization,including mineral dissolution and weathering(19.4%),localised Cd(16%),Ni(14.6%),Pb(12.8%),and Fe(11.4%)contamination from urbanisation while unidentified sources of pollution account for about 26.0%.The stable isotopes revealed groundwater is of meteoric origin and water-rock interaction the major mechanism for groundwater mineralization.The results of this research highlight the need of implementing an integrated strategy for managing and accessing groundwater quality.展开更多
The Jalilabad Cu(±Au)deposit lies in the central section of the Tarom-Hashjin Metallogenic Belt,in northern Zanjan Province,NW Iran.Mineralization predominantly occurs within quartz-sulfide veins,veinlets,and bre...The Jalilabad Cu(±Au)deposit lies in the central section of the Tarom-Hashjin Metallogenic Belt,in northern Zanjan Province,NW Iran.Mineralization predominantly occurs within quartz-sulfide veins,veinlets,and breccia zones,primarily hosted by the Eocene volcanic and volcaniclastic units of the Karaj Formation.The mineralization trends NW-SE and is influenced by several strike-slip faults.Chalcopyrite and bornite are the principal hypogene sulfides,with chalcocite and covellite representing the supergene stage.The post-ore stage is characterized by brecciated calcite and quartz.Geochemical analyses show that the monzonite intrusion contains SiO_(2)levels ranging from 69.80 to 70.24 wt.%,K_(2)O+Na_(2)O values between 8.10 and 8.15 wt.%,and K_(2)O/Na_(2)O ratios of 1.36 to 1.61.The intrusion is enriched in light rare earth elements(LREEs)and large-ion lithophile elements(LILEs)while being depleted in high-field-strength elements(HFSEs).A low Hf/Sm ratio indicates an orogenic-related magma,and a low Nb/La ratio points to a depleted mantle source.Microthermometric studies of three quartz types reveal moderate to high formation temperatures(195.4-322.7℃)and salinities ranging from 8.10 to 11.82 wt.%NaCl_(equiv).Oxygen isotope data(δ^(18)O_(H2O)O)range from+4.8‰to+8.1‰,suggesting a magmatic origin for the ore-forming fluids,later diluted by meteoric water.Sulfur isotope values(δ^(34)S_(H2S))between−6.0‰and−9.1‰confirm a magmatic source.Fluid mixing and dilution are identified as the primary mechanisms for ore precipitation.Raman spectroscopy enables nondestructive identification of minerals through their unique vibrational peaks.Chalcopyrite(213,280,1304 cm^(−1)),hematite(214,282,469,689,1309 cm^(−1)),goethite(967 cm^(−1)),and quartz(125,198,458 cm^(−1))show distinct spectral fingerprints indicating mineral differentiation,alteration tracking,and structural analysis in geological studies.Based on its geological context,the Jalilabad Cu(±Au)deposit is interpreted as resembling a high-sulfidation epithermal deposit.展开更多
Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a...Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a typical semi-arid tributary in the upper Yellow River basin.This study aimed to investigate the spatiotemporal patterns and sources of NO_(3)^(-)in the basin using an integrated analysis of hydrochemistry,stable isotopes,and dissolved organic matter(DOM)fluorescence.Hydrochemical results revealed that the water quality exhibited distinct seasonal variations,influenced by the unique hydrological and climatic conditions of the upper Yellow River.The surface water showed elevated concentrations of NO_(3)^(-)with limited denitrification,and NO_(3)^(-)accumulation was driven by multiple sources.Based on the dual isotopes(δ^(15)N andδ^(18)O)and the MixSIAR model,it was quantitatively determined that allochthonous inputs—including chemical nitrogen fertilizer,soil nitrogen,domestic sewage,and manure—constituted the primary sources of NO_(3)^(-).DOM fluorescence analysis revealed active biological or microbial metabolic activities,while allochthonous DOM significantly contributed to NO_(3)^(-)contamination.Both the MixSIAR model and DOM fluorescence results confirmed that protein-like substances were mainly derived from domestic sewage,whereas humic-like substances originated from non-point source pollution in the study area.These findings demonstrate the feasibility of combining isotopic and DOM fluorescence approaches to trace NO_(3)^(-)sources in surface water.This integrated methodology can support the design of targeted zonal management strategies to protect the surface water environment and maintain sustainable socioeconomic systems in semi-arid regions.展开更多
Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic...Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.展开更多
Stable isotope technology,as an important analytical tool,has been widely applied in the field of fisheries in China in recent years.This article summarizes the basic principles and research status of stable isotope t...Stable isotope technology,as an important analytical tool,has been widely applied in the field of fisheries in China in recent years.This article summarizes the basic principles and research status of stable isotope technology,with a focus on its application in China's fisheries research and field,including fisheries resource assessment,aquatic product traceability,and aquaculture ecosystem research.At the same time,the problems existing in the application process of this technology were analyzed,and the future development direction was discussed.By comprehensively elaborating on the application of stable isotope technology in the field of fisheries,the aim is to provide reference for relevant researchers and practitioners,and promote the further development and application of this technology in the field of fisheries.展开更多
Based on mass balance theory and IsoSource program,stable carbon and nitrogen isotopic ratios revealed that small mammals (plateau pika,root vole and plateau zokor) contributed 26.8% and 27.0% and 29.2% to alpine weas...Based on mass balance theory and IsoSource program,stable carbon and nitrogen isotopic ratios revealed that small mammals (plateau pika,root vole and plateau zokor) contributed 26.8% and 27.0% and 29.2% to alpine weasel,steppe polecat and upland buzzard of carnivores as food respectively;adult passerine birds contributed 22.3%,47.7% and 69.1%,with hatchlings contributing 50.9%,25.6% and 1.70% to each respectively.δ 13 C values plotted against δ 15 N indicated significant partitioning in two-dimensional space among the three carnivores.It was reasonable to propose a food resource partitioning among alpine weasel,steppe polecat and upland buzzard,which partially revealed their co-existence mechanisms.展开更多
Some key terms and relevant standard related with stable isotope tech- nique were introduced, and the determination of carbon, hydrogen and oxygen, the three stable isotopes in water, as well as their isotope effect w...Some key terms and relevant standard related with stable isotope tech- nique were introduced, and the determination of carbon, hydrogen and oxygen, the three stable isotopes in water, as well as their isotope effect was discussed. Appli- cations and advances of stable isotopes were stated mainly from plant water source, water use efficiency, the most active water adsorption region in root system and water resources utilization differences among different species in plant communi- ty. In addition, some existing problems in investigating the plant-water relations by using stable isotope technique were put forward. In this study, we aimed to promote the sustainable development of stable isotope technique in the research of plant water utilization and in the field of ecology in China.展开更多
The Panzhihua intrusion in southwest China is part of the Emeishan Large Igneous Province and host of a large Fe-Ti-V ore deposit.During emplacement of the main intrusion,multiple generations of mafic dykes invaded ca...The Panzhihua intrusion in southwest China is part of the Emeishan Large Igneous Province and host of a large Fe-Ti-V ore deposit.During emplacement of the main intrusion,multiple generations of mafic dykes invaded carbonate wall rocks,producing a large contact aureole.We measured the oxygen-isotope composition of the intrusions,their constituent minerals,and samples of the country rock.Magnetite and plagioclase from Panzhihua intrusion haveδ18O values that are consistent with magmatic equilibrium, and formed from magmas withδ18O values that were 1-2‰higher than expected in a mantle-derived magma.The unmetamorphosed country rock has highδ18O values,ranging from 13.2‰（sandstone） to 24.6-28.6‰（dolomite）.The skarns and marbles from the aureole have lowerδ18O andδ13C values than their protolith suggesting interaction with fluids that were in exchange equilibrium with the adjacent mafic magmas and especially the numerous mafic dykes that intruded the aureole.This would explain the alteration ofδ18O of the dykes which have significantly higher values than expected for a mantle-derived magma.Depending on the exactδ18O values assumed for the magma and contaminant, the amount of assimilation required to produce the elevatedδ18O value of the Panzhihua intrusion was between 8 and 13.7 wt.%,assuming simple mixing.The exact mechanism of contamination is unclear but may involve a combination of assimilation of bulk country rock,mixing with a melt of the country rock and exchange with CO2-rich fluid derived from decarbonation of the marls and dolomites.These mechanisms,particularly the latter,were probably involved in the formation of the Fe-Ti-V ores.展开更多
The Bangbu gold deposit is a large orogenic gold deposit in Xizang formed during the AlpineHimalayan collision.Ore bodies(auriferous quartz veins)are controlled by the E-W-trending Qusong-Cuogu-Zhemulang brittle-ducti...The Bangbu gold deposit is a large orogenic gold deposit in Xizang formed during the AlpineHimalayan collision.Ore bodies(auriferous quartz veins)are controlled by the E-W-trending Qusong-Cuogu-Zhemulang brittle-ductile shear zone.Quartz veins at the deposit can be divided into three types:pre-metallogenic hook-like quartz veins,metallogenic auriferous quartz veins,and postmetallogenic N-S quartz veins.Four stages of mineralization in the auriferous quartz veins have been identified:(1)Stage S1 quartz+coarse-grained sulfides,(2)Stage S2 gold+fine-grained sulfides,(3)Stage S3 quartz+carbonates,and(4)Stage S4 quartz+greigite.Fluid inclusions indicate the oreforming fluid was CO_2-N_2-CH_4 rich with homogenization temperatures of 170–261°C,salinities 4.34–7.45 wt%Na Cl equivalent.δ^(18)Ofluid(3.98‰–7.18‰)and lowδDV-SMOW(-90‰to-44‰)for auriferous quartz veins suggest ore-forming fluids were mainly metamorphic in origin,with some addition of organic matter.Quartz vein pyrite hasδ^(34)SV-CDT values of 1.2‰–3.6‰(an average of 2.2‰),whereas pyrite from phyllite hasδ^(34)SV-CDT 5.7‰–9.9‰(an average of 7.4‰).Quartz vein pyrites yield 206Pb/204 Pb ratios of 18.662–18.764,207Pb/204 Pb 15.650–15.683,and^(208)Pb/204 Pb 38.901–39.079.These isotopic data indicate Bangbu ore-forming materials were probably derived from the Langjiexue accretionary wedge.40Ar/39 Ar ages for sericite from auriferous sulfide-quartz veins yield a plateau age of 49.52±0.52 Ma,an isochron age of 50.3±0.31 Ma,suggesting that auriferous veins were formed during the main collisional period of the Xizang-Himalayan orogen(-65–41 Ma).展开更多
This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), m...This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), macroalgae, benthic microalgae and animals such as nematode and copepod. The stable isotope technique was used to quantify relative contributions of various sources to the food uptake by A. japonicus. The results showed that significant changes occurred in the C and N stable isotope values of sea cucumber food sources due to the seasonality of micro-or macroalgae prosperity and the fluctuation of environmental conditions. The sea cucumber A. japonicus exhibited corresponding alterations in feeding strategy in response to the changes in food conditions. Calculation with a stable isotope mixing model showed that macroalgae was the principal food source for A. japonicus throughout the 1-yr investigation, with the relative contribution averaging 28.1% - 63.2%. The relative contributions of other food sources such as copepod and nematode, POM, benthic microalgae to the total food uptake by sea cucumber averaged 22.6% - 39.1%, 6.3% - 22.2%, 2.8% - 6.5%, and 2.8% - 4.2%, respectively. Together these results indicated that the seasonal changes in food sources led to the obvious temporal differences in the relative contribution of various food sources utilized by A. japonicus. Such findings provide the basic scientific information for improving the aquaculture techniques of A. japonicus, particularly for optimizing the food environment of A. japonicus culture in farm ponds.展开更多
Crop root system plays an important role in the water cycle of the soil-plant-atmosphere continuum. In this study, com- bined isotope techniques, root length density and root cell activity analysis were used to invest...Crop root system plays an important role in the water cycle of the soil-plant-atmosphere continuum. In this study, com- bined isotope techniques, root length density and root cell activity analysis were used to investigate the root water uptake mechanisms of winter wheat （Triticum aesfivum L.） under different irrigation depths in the North China Plain. Both direct inference approach and multisource linear mixing model were applied to estimate the distribution of water uptake with depth in six growing stages. Results showed that winter wheat under land surface irrigation treatment （Ts） mainly absorbed water from 10-20 cm soil layers in the wintering and green stages （66.9 and 72.0%, respectively）; 0-20 cm （57.0%） in the jointing stage; 0-40 （15.3%） and 80-180 cm （58.1%） in the heading stage; 60-80 （13.2%） and 180-220 cm （35.5%） in the filling stage; and 0-40 （46.8%） and 80-100 cm （31.0%） in the ripening stage. Winter wheat under whole soil layers irrigation treatment （Tw） absorbed more water from deep soil layer than Ts in heading, filling and ripening stages. Moreover, root cell activity and root length density of winter wheat under TW were significantly greater than that of Ts in the three stages. We concluded that distribution of water uptake with depth was affected by the availability of water sources, the root length density and root cell activity. Implementation of the whole soil layers irrigation method can affect root system distribution and thereby increase water use from deeper soil and enhance water use efficiency.展开更多
In order to investigate the transformation among the precipitation,groundwater,and surface water in the Sanjiang Plain,Northeast China,precipitation and groundwater samples which were collected at the meteorological s...In order to investigate the transformation among the precipitation,groundwater,and surface water in the Sanjiang Plain,Northeast China,precipitation and groundwater samples which were collected at the meteorological station of the Sanjiang Mire Wetland Experimental Station,Chinese Academy of Sciences and the surface water which collected from the Wolulan River were used to identify the transformation of three types of water.The isotope composition of different kinds of water sources were analyzed via stable isotope(deuterium and oxygen-18) investigation of natural water.The results show a clear seasonal difference in the stable isotopes in precipitation.During the cold half-year,the mean stable isotope in precipitation in the Sanjiang Plain reaches its minimum with the minimum temperature.The δ18O and δD values are high in the rainy season.In the Wolulan River,the evaporation is the highest in August and September.The volume of evaporation and the replenishment to the river is mostly same.The groundwater is recharged more by the direct infiltration of precipitation than by the river flow.The results of this study indicate that the water bodies in the Sanjiang Plain have close hydrologic relationships,and that the transformation among each water system frequently occurs.展开更多
Carbon and nitrogen stable isotopes are useful tracers for distinguishing marine and terrestrial plant sources of sedimentary organic matter(OM),and for identifying OM from different types of plants.By analyzing the c...Carbon and nitrogen stable isotopes are useful tracers for distinguishing marine and terrestrial plant sources of sedimentary organic matter(OM),and for identifying OM from different types of plants.By analyzing the carbon and nitrogen stable isotopes of marine and riverine sediments from Bohai Bay and its catchment,we were able to identify the source of OM in these sediments.The stable carbon isotope values of Bohai Bay sediments were between-22.94‰ and-23.90‰,while those of riverine sediments were from-24.45‰ to-32.50‰.Marine algae were the main source of OM in Bohai Bay sediments.However,lacustrine algae were the main source of riverine sediments,not terrestrial OM.The nitrogen isotopes in Bohai Bay sediments decreased in eastward direction,with increasing distance from the coastline,which suggested a higher degree of impact from human activities along the coast.展开更多
Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution chara...Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.展开更多
Under the increasing pressure of water shortage and steppe degradation, information on the hydrological cycle in steppe region in Inner Mongolia, China is urgently needed. An intensive investigation of the temporal va...Under the increasing pressure of water shortage and steppe degradation, information on the hydrological cycle in steppe region in Inner Mongolia, China is urgently needed. An intensive investigation of the temporal varia-tions of δD and δ^18O in precipitation was conducted in 2007-2008 in the Xilin River Basin, Inner Mongolia in the northern China. The 6D and δ^18O values for 54 precipitation samples range from ＋1.1%o to -34.7%0 and -3.0%0 to -269%0, respectively. This wide range indicates that stable isotopes in precipitation are primarily controlled by differ-ent condensation mechanisms as a function of air temperature and varying sources of vapor. The relationship between δD and δ^18O defined a well constrained line given by δD = 7.896180 ＋ 9.5, which is nearly identical to the Meteoric Water Line in the northern China. The temperature effect is clearly displayed in this area. The results of backward tra-jectory of each precipitation day show that the vapor of the precipitation in cold season （October to March） mainly originates from the west while the moisture source is more complicated in warm season （April to September）. A light precipitation amount effect existes at the precipitation event scale in this area. The vapor source of precipitation with higher d-excesses are mainly from the west wind or neighboring inland area and precipitation with lower d-excesses from a monsoon source from the southeastern China.展开更多
Under Rayleigh equilibrium condition, stable isotopic ratio in residual water increases with the decrease of the residual water proportion f exponentially, and the fractionation rate of stable isotopes is inversely pr...Under Rayleigh equilibrium condition, stable isotopic ratio in residual water increases with the decrease of the residual water proportion f exponentially, and the fractionation rate of stable isotopes is inversely proportional to temperature. However, under kinetic evaporation condition, the fi＇actionation of stable isotopes is not only related to the phase temperature but also influenced by the atmospheric humidity and the mass exchange between liquid and vapor phases. The ratio 6 in residual water will not change with f after undergoing evaporation of a long time for great relative humidity. The rate that the evaporating water body reaches isotopic steady state is mainly dependent on the relative humidity in atmosphere. The analysis shows that the actual mean linear variety rates, about -30.0, of the δ^18O in residual water versus the residual water proportion at Nagqu and Amdo stations are consistent with the simulated process under temperature of 20℃ and relative humidity of 50%. The distillation line simulated under Rayleigh equilibrium condition is analogous to the global meteoric water line （MWL） as the temperature is about 20℃. Under non-equilibrium condition, the slope and constant values of distillation line are directly proportional to temperature and relative humidity. According to the basic data, the simulated distillation line is very consistent with the actual distillation line of Qinghai Lake.展开更多
The biokinetics of Cu in Daphnia magna, including dissolved uptake, assimilation and efflux, has been determined using a gamma 67^Cu radiotracer methodology. However, this gamma emitting radioisotope is not readily av...The biokinetics of Cu in Daphnia magna, including dissolved uptake, assimilation and efflux, has been determined using a gamma 67^Cu radiotracer methodology. However, this gamma emitting radioisotope is not readily available due to its very short half-life. In the present study, we employed a stable isotope tracer （65^Cu） to determine the Cu biokinetics and compared our results to those determined using 67^Cu. The dissolved uptake rate constant of 65^Cu was 3.36 L/（g.day）, which is higher than that of 67^Cu （1.32 L/（g.day））. With increasing food concentrations from 2×10^4 to 1×10^5 cells/mL, the Cu assimilation efficiency （AE） decreased from 46% to 11%, compared to a decrease from 27% to 16% when determined using 67^Cu. The effiux of Cu from Daphnia magna was quantified following both dissolved and dietary uptake. The efflux of waterborne Cu was comparable to that of dietborne Cu and the effiux rate constant （0.32-0.52 day^-l） was higher than that determined by 67^Cu （0.19-0.20 day^-1）. By considering different water properties and handling procedure between the two experiments, we believe that these differences are reasonable. Overall, this study demonstrated that the enriched stable isotope tracer technique is a powerful tool to investigate metal bioavailability and maybe a good alternative to radioactive measurements.展开更多
As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five repre...As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five representative species caught by the Chinese longline fleet in the mid-east Pacific, i.e., the blue shark （Prionace glauca）, the bigeye thresher shark （Alopias superciliosus）, the silky shark （Carcharhinus falciformis）, the scalloped hammerhead （Sphyrna lewini）, and the oceanic whitetip shark （Car-charhinus longimanus）. Of these species, oceanic whitetip shark has the lowest trophic level and mean 815N value （3.9 and 14.93%o± 0.84%o）, whereas bigeye thresher shark has the highest level/values （4.5 and 17.02%o±1.21%o, respectively）. The bigeye thresher shark has significantly higher 515N value than other shark species, indicating its higher trophic position. The blue shark and oceanic whitetip shark has significantly higher 813C values than bigeye thresher shark, silky shark and scalloped hammerhead, possibly due to different diets and/or living habitats. The stable isotope data and stomach content data are highly consistent, suggesting that sta-ble isotope analysis supplements traditional feeding ecology study of sharks, and thus contributes to understanding their trophic linkage.展开更多
Based on stable isotope analysis, we characterized the dietary regime of the Manila clam Ruditapesphilippinarum inhabiting intertidal areas along the Liaodong Peninsula, Northern China. Samples, including particulate ...Based on stable isotope analysis, we characterized the dietary regime of the Manila clam Ruditapesphilippinarum inhabiting intertidal areas along the Liaodong Peninsula, Northern China. Samples, including particulate organic matter （POM; n=30）, benthic microalgae （BMI; n=30） and R. philippinarum （n=60）, were collected from six sampling sites displaying the same ecological conditions. Of the two primary food sources, POM was more depleted in δ13C （-20.61‰ to -22.89%0） than BMI was （-13.90‰ to -16.66‰）. With respect to 15N, BMI was more enriched （2.90%0 to 4.07%0） than POM was （4.13‰ to 5.12‰）. The δ13C values of R. philippinarum ranged from -18.78‰ to -19.35‰ and the δ15N values from 7.96%0 to 8.63‰, which were intermediate between the POM and BMI values. In a two-source isotope mixing model, we estimated the relative contributions of POM and BMI to the diet ofR. philippinarum to be 74.2% and 25.8%, respectively. We conclude that R. philippinarum feeds mainly on POM, and BMI is also an important supplemental food source in intertidal areas.展开更多
Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive r...Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive results for analysis of amino acids.In this work,we utilized a stable isotope derivatization method,combining with liquid chromatography tandem mass spectrometry(SID-LC-MS),to improve the specificity for screening amino acids in DBS specimens.A pair of isotope reagents,p-(dimethylamino)phenyl isothiocyanate(DMAP-NCS) and 4-isothiocyanato-N,N-bis(methyl-[2H2])aniline([2H4]DMAP-NCS),was synthesized and used to label amino acids in DBS specimens.The [2H4]DMAP-NCS labelled amino acid standards were used as internal standards to compensate the matrix effect.This method was validated by measuring linearity,recovery and accuracy.The results showed that the developed SID-LC-MS method can be used for sensitive and selective determination of 12 diagnostically important amino acids in DBS specimens.展开更多
文摘In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes controlling the groundwater chemistry is a major step in the sustainable management of the aquifers.A total of 29groundwater samples were collected and analysed.Ionic ratio graphs,multivariate statistical analysis,mineral saturation indices,stable isotopes,and geostatistics methods were used to examine the sources and the quality of the groundwater.The findings describe the water types in the district as Ca-Mg-HCO_(3)-Cl,Ca-Na-HCO_(3),Na-Ca-HCO_(3),Ca-Na-HCO_(3)-Cl,Na-Ca-HCO_(3)-Cl,mix water type,NaHCO_(3)-Cl,with possible evolution to Ca-Na-Cl-HCO_(3),and Na-Ca-Cl-HCO_(3).According to the IEWQI for drinking water,around 53.6% of the samples have good quality,whereas 10.7% have very low-quality groundwater.Only 3.45% of the samples are suitable to use for irrigation without treatment,whereas 41.4% are somewhat safe with minimal treatment.Water-rock interactions,including the dissolution and weathering of silicate minerals,cation exchange processes,and human activities like mining andquarrying,are some of the main factors influencing groundwater chemistry.Principal component analysis revealed that groundwater chemistry is influenced by a combination of natural and anthropogenic sources.The APCs-MLR receptor model quantifies the factors that play important roles in groundwater salinization,including mineral dissolution and weathering(19.4%),localised Cd(16%),Ni(14.6%),Pb(12.8%),and Fe(11.4%)contamination from urbanisation while unidentified sources of pollution account for about 26.0%.The stable isotopes revealed groundwater is of meteoric origin and water-rock interaction the major mechanism for groundwater mineralization.The results of this research highlight the need of implementing an integrated strategy for managing and accessing groundwater quality.
文摘The Jalilabad Cu(±Au)deposit lies in the central section of the Tarom-Hashjin Metallogenic Belt,in northern Zanjan Province,NW Iran.Mineralization predominantly occurs within quartz-sulfide veins,veinlets,and breccia zones,primarily hosted by the Eocene volcanic and volcaniclastic units of the Karaj Formation.The mineralization trends NW-SE and is influenced by several strike-slip faults.Chalcopyrite and bornite are the principal hypogene sulfides,with chalcocite and covellite representing the supergene stage.The post-ore stage is characterized by brecciated calcite and quartz.Geochemical analyses show that the monzonite intrusion contains SiO_(2)levels ranging from 69.80 to 70.24 wt.%,K_(2)O+Na_(2)O values between 8.10 and 8.15 wt.%,and K_(2)O/Na_(2)O ratios of 1.36 to 1.61.The intrusion is enriched in light rare earth elements(LREEs)and large-ion lithophile elements(LILEs)while being depleted in high-field-strength elements(HFSEs).A low Hf/Sm ratio indicates an orogenic-related magma,and a low Nb/La ratio points to a depleted mantle source.Microthermometric studies of three quartz types reveal moderate to high formation temperatures(195.4-322.7℃)and salinities ranging from 8.10 to 11.82 wt.%NaCl_(equiv).Oxygen isotope data(δ^(18)O_(H2O)O)range from+4.8‰to+8.1‰,suggesting a magmatic origin for the ore-forming fluids,later diluted by meteoric water.Sulfur isotope values(δ^(34)S_(H2S))between−6.0‰and−9.1‰confirm a magmatic source.Fluid mixing and dilution are identified as the primary mechanisms for ore precipitation.Raman spectroscopy enables nondestructive identification of minerals through their unique vibrational peaks.Chalcopyrite(213,280,1304 cm^(−1)),hematite(214,282,469,689,1309 cm^(−1)),goethite(967 cm^(−1)),and quartz(125,198,458 cm^(−1))show distinct spectral fingerprints indicating mineral differentiation,alteration tracking,and structural analysis in geological studies.Based on its geological context,the Jalilabad Cu(±Au)deposit is interpreted as resembling a high-sulfidation epithermal deposit.
基金The Basic Special Business Fund for Research and Development for the Central Level Scientific Research Institutes,Nanjing Institute of Environmental Sciences,MEE,Grant/Award Number:GYZX240103The Innovative Team Project of Nanjing Institute of Environmental Sciences,MEE,Grant/Award Number:GYZX200101Geological Prospecting Funds Program of Zhejiang Province,China,Grant/Award Number:No.2023021。
文摘Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a typical semi-arid tributary in the upper Yellow River basin.This study aimed to investigate the spatiotemporal patterns and sources of NO_(3)^(-)in the basin using an integrated analysis of hydrochemistry,stable isotopes,and dissolved organic matter(DOM)fluorescence.Hydrochemical results revealed that the water quality exhibited distinct seasonal variations,influenced by the unique hydrological and climatic conditions of the upper Yellow River.The surface water showed elevated concentrations of NO_(3)^(-)with limited denitrification,and NO_(3)^(-)accumulation was driven by multiple sources.Based on the dual isotopes(δ^(15)N andδ^(18)O)and the MixSIAR model,it was quantitatively determined that allochthonous inputs—including chemical nitrogen fertilizer,soil nitrogen,domestic sewage,and manure—constituted the primary sources of NO_(3)^(-).DOM fluorescence analysis revealed active biological or microbial metabolic activities,while allochthonous DOM significantly contributed to NO_(3)^(-)contamination.Both the MixSIAR model and DOM fluorescence results confirmed that protein-like substances were mainly derived from domestic sewage,whereas humic-like substances originated from non-point source pollution in the study area.These findings demonstrate the feasibility of combining isotopic and DOM fluorescence approaches to trace NO_(3)^(-)sources in surface water.This integrated methodology can support the design of targeted zonal management strategies to protect the surface water environment and maintain sustainable socioeconomic systems in semi-arid regions.
基金supported by the Scientific Research Foundation of Hainan Tropical Ocean University(No.RHDRC202312)the Major Science and Technology Project of Yazhou Bay Innovation Institute of Hainan Tropical Ocean University:Research on Hainan Coastal Marine Ecological Environment and Carbon Sink(No.2022CXYZD002).
文摘Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.
基金supported by the National Modern Agricultural Industry Technology System(CARS-45-33)Innovation Team of Tianjin Freshwater Aquaculture Industry Technology System(ITTFRS2021000-002 and ITTFRS2021000-001)+1 种基金Tianjin Science and Technology Plan Project(24KPHDRC00280,24ZYCGSN00250 and 23YDTPJC00420)the Open Fund Project of Key Laboratory of Ocean Observation Technology,MNR(2023klootA03).
文摘Stable isotope technology,as an important analytical tool,has been widely applied in the field of fisheries in China in recent years.This article summarizes the basic principles and research status of stable isotope technology,with a focus on its application in China's fisheries research and field,including fisheries resource assessment,aquatic product traceability,and aquaculture ecosystem research.At the same time,the problems existing in the application process of this technology were analyzed,and the future development direction was discussed.By comprehensively elaborating on the application of stable isotope technology in the field of fisheries,the aim is to provide reference for relevant researchers and practitioners,and promote the further development and application of this technology in the field of fisheries.
文摘Based on mass balance theory and IsoSource program,stable carbon and nitrogen isotopic ratios revealed that small mammals (plateau pika,root vole and plateau zokor) contributed 26.8% and 27.0% and 29.2% to alpine weasel,steppe polecat and upland buzzard of carnivores as food respectively;adult passerine birds contributed 22.3%,47.7% and 69.1%,with hatchlings contributing 50.9%,25.6% and 1.70% to each respectively.δ 13 C values plotted against δ 15 N indicated significant partitioning in two-dimensional space among the three carnivores.It was reasonable to propose a food resource partitioning among alpine weasel,steppe polecat and upland buzzard,which partially revealed their co-existence mechanisms.
基金Supported by the Special Fund for the Innovation Capacity Platform Construction of Guizhou Province([2012]040030)~~
文摘Some key terms and relevant standard related with stable isotope tech- nique were introduced, and the determination of carbon, hydrogen and oxygen, the three stable isotopes in water, as well as their isotope effect was discussed. Appli- cations and advances of stable isotopes were stated mainly from plant water source, water use efficiency, the most active water adsorption region in root system and water resources utilization differences among different species in plant communi- ty. In addition, some existing problems in investigating the plant-water relations by using stable isotope technique were put forward. In this study, we aimed to promote the sustainable development of stable isotope technique in the research of plant water utilization and in the field of ecology in China.
基金The project benefited from a PROCORE Hong Kong-France exchange grant to Arndt and Zhou and a grant from the US National Science Foundation
文摘The Panzhihua intrusion in southwest China is part of the Emeishan Large Igneous Province and host of a large Fe-Ti-V ore deposit.During emplacement of the main intrusion,multiple generations of mafic dykes invaded carbonate wall rocks,producing a large contact aureole.We measured the oxygen-isotope composition of the intrusions,their constituent minerals,and samples of the country rock.Magnetite and plagioclase from Panzhihua intrusion haveδ18O values that are consistent with magmatic equilibrium, and formed from magmas withδ18O values that were 1-2‰higher than expected in a mantle-derived magma.The unmetamorphosed country rock has highδ18O values,ranging from 13.2‰（sandstone） to 24.6-28.6‰（dolomite）.The skarns and marbles from the aureole have lowerδ18O andδ13C values than their protolith suggesting interaction with fluids that were in exchange equilibrium with the adjacent mafic magmas and especially the numerous mafic dykes that intruded the aureole.This would explain the alteration ofδ18O of the dykes which have significantly higher values than expected for a mantle-derived magma.Depending on the exactδ18O values assumed for the magma and contaminant, the amount of assimilation required to produce the elevatedδ18O value of the Panzhihua intrusion was between 8 and 13.7 wt.%,assuming simple mixing.The exact mechanism of contamination is unclear but may involve a combination of assimilation of bulk country rock,mixing with a melt of the country rock and exchange with CO2-rich fluid derived from decarbonation of the marls and dolomites.These mechanisms,particularly the latter,were probably involved in the formation of the Fe-Ti-V ores.
基金funded by the National Basic Research Program of China(No.2011CB403104)Geological Survey Project of China(No.12120113037901)
文摘The Bangbu gold deposit is a large orogenic gold deposit in Xizang formed during the AlpineHimalayan collision.Ore bodies(auriferous quartz veins)are controlled by the E-W-trending Qusong-Cuogu-Zhemulang brittle-ductile shear zone.Quartz veins at the deposit can be divided into three types:pre-metallogenic hook-like quartz veins,metallogenic auriferous quartz veins,and postmetallogenic N-S quartz veins.Four stages of mineralization in the auriferous quartz veins have been identified:(1)Stage S1 quartz+coarse-grained sulfides,(2)Stage S2 gold+fine-grained sulfides,(3)Stage S3 quartz+carbonates,and(4)Stage S4 quartz+greigite.Fluid inclusions indicate the oreforming fluid was CO_2-N_2-CH_4 rich with homogenization temperatures of 170–261°C,salinities 4.34–7.45 wt%Na Cl equivalent.δ^(18)Ofluid(3.98‰–7.18‰)and lowδDV-SMOW(-90‰to-44‰)for auriferous quartz veins suggest ore-forming fluids were mainly metamorphic in origin,with some addition of organic matter.Quartz vein pyrite hasδ^(34)SV-CDT values of 1.2‰–3.6‰(an average of 2.2‰),whereas pyrite from phyllite hasδ^(34)SV-CDT 5.7‰–9.9‰(an average of 7.4‰).Quartz vein pyrites yield 206Pb/204 Pb ratios of 18.662–18.764,207Pb/204 Pb 15.650–15.683,and^(208)Pb/204 Pb 38.901–39.079.These isotopic data indicate Bangbu ore-forming materials were probably derived from the Langjiexue accretionary wedge.40Ar/39 Ar ages for sericite from auriferous sulfide-quartz veins yield a plateau age of 49.52±0.52 Ma,an isochron age of 50.3±0.31 Ma,suggesting that auriferous veins were formed during the main collisional period of the Xizang-Himalayan orogen(-65–41 Ma).
基金funded by the National Natural Science Foundation of China (Grant Nos.31172426 and 30871931)the Ministry of Science and Technology of China (Grant Nos.2011BAD13B03 and JQ201009)+1 种基金the State Oceanic Administration of China (Grant No.200905020)supported by the Program for New Century Excellent Talents in University
文摘This study investigated the seasonal changes in carbon (C) and nitrogen (N) stable isotope values of several typical food sources of Apostichopus japonicus in a farm pond, including particulate organic matter (POM), macroalgae, benthic microalgae and animals such as nematode and copepod. The stable isotope technique was used to quantify relative contributions of various sources to the food uptake by A. japonicus. The results showed that significant changes occurred in the C and N stable isotope values of sea cucumber food sources due to the seasonality of micro-or macroalgae prosperity and the fluctuation of environmental conditions. The sea cucumber A. japonicus exhibited corresponding alterations in feeding strategy in response to the changes in food conditions. Calculation with a stable isotope mixing model showed that macroalgae was the principal food source for A. japonicus throughout the 1-yr investigation, with the relative contribution averaging 28.1% - 63.2%. The relative contributions of other food sources such as copepod and nematode, POM, benthic microalgae to the total food uptake by sea cucumber averaged 22.6% - 39.1%, 6.3% - 22.2%, 2.8% - 6.5%, and 2.8% - 4.2%, respectively. Together these results indicated that the seasonal changes in food sources led to the obvious temporal differences in the relative contribution of various food sources utilized by A. japonicus. Such findings provide the basic scientific information for improving the aquaculture techniques of A. japonicus, particularly for optimizing the food environment of A. japonicus culture in farm ponds.
基金supported by the National Natural Science Foundation of China(50979065,51109154 and 51249002)the Natural Science Foundation of Shanxi Province,China(2012021026-2)+2 种基金the Program for Science and Technology Development of Shanxi Province,China(20110311018-1)the Specialized Research Fund for the Doctoral Program of Higher Education,China(20111402120006,20121402110009)the Program for Graduate Student Education and Innovation of Shanxi Province,China(2015BY27)
文摘Crop root system plays an important role in the water cycle of the soil-plant-atmosphere continuum. In this study, com- bined isotope techniques, root length density and root cell activity analysis were used to investigate the root water uptake mechanisms of winter wheat （Triticum aesfivum L.） under different irrigation depths in the North China Plain. Both direct inference approach and multisource linear mixing model were applied to estimate the distribution of water uptake with depth in six growing stages. Results showed that winter wheat under land surface irrigation treatment （Ts） mainly absorbed water from 10-20 cm soil layers in the wintering and green stages （66.9 and 72.0%, respectively）; 0-20 cm （57.0%） in the jointing stage; 0-40 （15.3%） and 80-180 cm （58.1%） in the heading stage; 60-80 （13.2%） and 180-220 cm （35.5%） in the filling stage; and 0-40 （46.8%） and 80-100 cm （31.0%） in the ripening stage. Winter wheat under whole soil layers irrigation treatment （Tw） absorbed more water from deep soil layer than Ts in heading, filling and ripening stages. Moreover, root cell activity and root length density of winter wheat under TW were significantly greater than that of Ts in the three stages. We concluded that distribution of water uptake with depth was affected by the availability of water sources, the root length density and root cell activity. Implementation of the whole soil layers irrigation method can affect root system distribution and thereby increase water use from deeper soil and enhance water use efficiency.
基金Under the auspices of Major Science and Technology Program for Water Pollution Control and Treatment(No.2012ZX07201004)National Natural Science Foundation of China(No.41101470)
文摘In order to investigate the transformation among the precipitation,groundwater,and surface water in the Sanjiang Plain,Northeast China,precipitation and groundwater samples which were collected at the meteorological station of the Sanjiang Mire Wetland Experimental Station,Chinese Academy of Sciences and the surface water which collected from the Wolulan River were used to identify the transformation of three types of water.The isotope composition of different kinds of water sources were analyzed via stable isotope(deuterium and oxygen-18) investigation of natural water.The results show a clear seasonal difference in the stable isotopes in precipitation.During the cold half-year,the mean stable isotope in precipitation in the Sanjiang Plain reaches its minimum with the minimum temperature.The δ18O and δD values are high in the rainy season.In the Wolulan River,the evaporation is the highest in August and September.The volume of evaporation and the replenishment to the river is mostly same.The groundwater is recharged more by the direct infiltration of precipitation than by the river flow.The results of this study indicate that the water bodies in the Sanjiang Plain have close hydrologic relationships,and that the transformation among each water system frequently occurs.
基金Supported by the National Natural Science Foundation of China(No.41273068)the Tianjin Research Program of Applied Science and Advanced Technology(No.11JCZDJC24100)
文摘Carbon and nitrogen stable isotopes are useful tracers for distinguishing marine and terrestrial plant sources of sedimentary organic matter(OM),and for identifying OM from different types of plants.By analyzing the carbon and nitrogen stable isotopes of marine and riverine sediments from Bohai Bay and its catchment,we were able to identify the source of OM in these sediments.The stable carbon isotope values of Bohai Bay sediments were between-22.94‰ and-23.90‰,while those of riverine sediments were from-24.45‰ to-32.50‰.Marine algae were the main source of OM in Bohai Bay sediments.However,lacustrine algae were the main source of riverine sediments,not terrestrial OM.The nitrogen isotopes in Bohai Bay sediments decreased in eastward direction,with increasing distance from the coastline,which suggested a higher degree of impact from human activities along the coast.
基金Under the auspices of Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-223)National Natural Science Foundation of China (No. 40803023)+1 种基金Key Program of Natural Science Foundation of Shandong Province(No. ZR2010DZ001)Talents Foundation of Chinese Academy of Sciences (No. AJ0809BX-036)
文摘Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.
基金Under the auspices of Nation Basic Research Program of China(No.2007CB411502)German Science Foundation(Research Unit 536)Independent Research Project from State Key Laboratory of Cryospheric Science(No.SKLCS-ZZ-2010-02)
文摘Under the increasing pressure of water shortage and steppe degradation, information on the hydrological cycle in steppe region in Inner Mongolia, China is urgently needed. An intensive investigation of the temporal varia-tions of δD and δ^18O in precipitation was conducted in 2007-2008 in the Xilin River Basin, Inner Mongolia in the northern China. The 6D and δ^18O values for 54 precipitation samples range from ＋1.1%o to -34.7%0 and -3.0%0 to -269%0, respectively. This wide range indicates that stable isotopes in precipitation are primarily controlled by differ-ent condensation mechanisms as a function of air temperature and varying sources of vapor. The relationship between δD and δ^18O defined a well constrained line given by δD = 7.896180 ＋ 9.5, which is nearly identical to the Meteoric Water Line in the northern China. The temperature effect is clearly displayed in this area. The results of backward tra-jectory of each precipitation day show that the vapor of the precipitation in cold season （October to March） mainly originates from the west while the moisture source is more complicated in warm season （April to September）. A light precipitation amount effect existes at the precipitation event scale in this area. The vapor source of precipitation with higher d-excesses are mainly from the west wind or neighboring inland area and precipitation with lower d-excesses from a monsoon source from the southeastern China.
基金N ational N atural Science Foundation of China, N o.40271025 the N ational H igh Technology Research andD evelopm ent Program of China (863 Program ), N o.2002A A 135360+1 种基金 the Program of Education D epartm ent ofH unan Province,N o.03C210the CA S Tianshan G laciologicalStation Foundation,N o.TZ2000-02
文摘Under Rayleigh equilibrium condition, stable isotopic ratio in residual water increases with the decrease of the residual water proportion f exponentially, and the fractionation rate of stable isotopes is inversely proportional to temperature. However, under kinetic evaporation condition, the fi＇actionation of stable isotopes is not only related to the phase temperature but also influenced by the atmospheric humidity and the mass exchange between liquid and vapor phases. The ratio 6 in residual water will not change with f after undergoing evaporation of a long time for great relative humidity. The rate that the evaporating water body reaches isotopic steady state is mainly dependent on the relative humidity in atmosphere. The analysis shows that the actual mean linear variety rates, about -30.0, of the δ^18O in residual water versus the residual water proportion at Nagqu and Amdo stations are consistent with the simulated process under temperature of 20℃ and relative humidity of 50%. The distillation line simulated under Rayleigh equilibrium condition is analogous to the global meteoric water line （MWL） as the temperature is about 20℃. Under non-equilibrium condition, the slope and constant values of distillation line are directly proportional to temperature and relative humidity. According to the basic data, the simulated distillation line is very consistent with the actual distillation line of Qinghai Lake.
基金supported by the National Basic Research Program of China "Water Environmental Quality Evolution and Water Quality Criteria in Lakes" (No.2008CB418201)the National Natural Science Foundation of China (No. 40871215)the Natural Science Foundation of Beijing (No. 8092019)
文摘The biokinetics of Cu in Daphnia magna, including dissolved uptake, assimilation and efflux, has been determined using a gamma 67^Cu radiotracer methodology. However, this gamma emitting radioisotope is not readily available due to its very short half-life. In the present study, we employed a stable isotope tracer （65^Cu） to determine the Cu biokinetics and compared our results to those determined using 67^Cu. The dissolved uptake rate constant of 65^Cu was 3.36 L/（g.day）, which is higher than that of 67^Cu （1.32 L/（g.day））. With increasing food concentrations from 2×10^4 to 1×10^5 cells/mL, the Cu assimilation efficiency （AE） decreased from 46% to 11%, compared to a decrease from 27% to 16% when determined using 67^Cu. The effiux of Cu from Daphnia magna was quantified following both dissolved and dietary uptake. The efflux of waterborne Cu was comparable to that of dietborne Cu and the effiux rate constant （0.32-0.52 day^-l） was higher than that determined by 67^Cu （0.19-0.20 day^-1）. By considering different water properties and handling procedure between the two experiments, we believe that these differences are reasonable. Overall, this study demonstrated that the enriched stable isotope tracer technique is a powerful tool to investigate metal bioavailability and maybe a good alternative to radioactive measurements.
基金Li Yunkai was supported by the National Natural Science Foundation of China (No.41206124)Ph.D.Programs Foundation of Ministry of Education of China (No.201 23104120001)+3 种基金the ‘Chen Guang’ Project of Shanghai Municipal Education Commission (No.D8004-10-0206)the Shanghai Education Development Foundation (No.B-8102-10-0084)Zhu Jiangfeng and Dai Xiaojie were supported by the National Natural Science Foundation of China (No.41106118)the Scientific Observing and Experimental Station of Oceanic Fishery Resources, Ministry of Agriculture, China
文摘As apex predators, sharks are of ecological and conservation importance in marine ecosystems. In this study, trophic positions of sharks were estimated using stable isotope ratios of carbon and nitrogen for five representative species caught by the Chinese longline fleet in the mid-east Pacific, i.e., the blue shark （Prionace glauca）, the bigeye thresher shark （Alopias superciliosus）, the silky shark （Carcharhinus falciformis）, the scalloped hammerhead （Sphyrna lewini）, and the oceanic whitetip shark （Car-charhinus longimanus）. Of these species, oceanic whitetip shark has the lowest trophic level and mean 815N value （3.9 and 14.93%o± 0.84%o）, whereas bigeye thresher shark has the highest level/values （4.5 and 17.02%o±1.21%o, respectively）. The bigeye thresher shark has significantly higher 515N value than other shark species, indicating its higher trophic position. The blue shark and oceanic whitetip shark has significantly higher 813C values than bigeye thresher shark, silky shark and scalloped hammerhead, possibly due to different diets and/or living habitats. The stable isotope data and stomach content data are highly consistent, suggesting that sta-ble isotope analysis supplements traditional feeding ecology study of sharks, and thus contributes to understanding their trophic linkage.
基金Supported by the Modern Agro-industry Technology Research System (No.CARS-48)the State Oceanic Administration of China (No.200905020)
文摘Based on stable isotope analysis, we characterized the dietary regime of the Manila clam Ruditapesphilippinarum inhabiting intertidal areas along the Liaodong Peninsula, Northern China. Samples, including particulate organic matter （POM; n=30）, benthic microalgae （BMI; n=30） and R. philippinarum （n=60）, were collected from six sampling sites displaying the same ecological conditions. Of the two primary food sources, POM was more depleted in δ13C （-20.61‰ to -22.89%0） than BMI was （-13.90‰ to -16.66‰）. With respect to 15N, BMI was more enriched （2.90%0 to 4.07%0） than POM was （4.13‰ to 5.12‰）. The δ13C values of R. philippinarum ranged from -18.78‰ to -19.35‰ and the δ15N values from 7.96%0 to 8.63‰, which were intermediate between the POM and BMI values. In a two-source isotope mixing model, we estimated the relative contributions of POM and BMI to the diet ofR. philippinarum to be 74.2% and 25.8%, respectively. We conclude that R. philippinarum feeds mainly on POM, and BMI is also an important supplemental food source in intertidal areas.
基金supported by the National Key R&D Program of China(No.2018YFA0900400)the National Natural ScienceFoundation of China(Nos.21635006,31670373,21721005,21904099)the Postdoctoral Science Foundation of China(No.2018M642893)。
文摘Direct infusion mass spectrometry(DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots(DBS).However,DIMS sometimes generated false-positive results for analysis of amino acids.In this work,we utilized a stable isotope derivatization method,combining with liquid chromatography tandem mass spectrometry(SID-LC-MS),to improve the specificity for screening amino acids in DBS specimens.A pair of isotope reagents,p-(dimethylamino)phenyl isothiocyanate(DMAP-NCS) and 4-isothiocyanato-N,N-bis(methyl-[2H2])aniline([2H4]DMAP-NCS),was synthesized and used to label amino acids in DBS specimens.The [2H4]DMAP-NCS labelled amino acid standards were used as internal standards to compensate the matrix effect.This method was validated by measuring linearity,recovery and accuracy.The results showed that the developed SID-LC-MS method can be used for sensitive and selective determination of 12 diagnostically important amino acids in DBS specimens.
