Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic...Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.展开更多
Biomarker and stable carbon isotope analyses are presented for the Garau Formation of the Cretaceous Age,an important source rock in western Iran,to reveal its potential as an oilprone source rock.The C_(28)/C_(29)ste...Biomarker and stable carbon isotope analyses are presented for the Garau Formation of the Cretaceous Age,an important source rock in western Iran,to reveal its potential as an oilprone source rock.The C_(28)/C_(29)sterane ratio value range(0.72 to 0.83)of bitumen samples from the formation suggests that they were likely formed during phytoplankton blooms.Sterane,hopane,and isoprenoid/n-alkane ratios indicate that the formation's organic matter predominantly consists of algae,and bacteria,accompanied by some reworked material derived from higher plants.Due to the predominance of anoxic conditions and the actions of sulfate-reducing bacteria,the bitumen present is enriched with sulfur compounds.The percentages of saturates,aromatics,and nitrogen-sulfuroxygen(NSO)fractions in the bitumen samples classify them as naphthenic oils.Isotope analysis reveals that biodegradation and water-washing have reduced the concentrations of some volatile saturates and low molecular weight aromatics in the bitumen samples.These actions have resulted in distinctiveδ^(13)C values for the formation's kerogen and bitumen fractions.The formation's organic matter has been subjected to high-temperature thermal regimes and has entered the oil-generation window at the sampled localities,with vitrinite reflectance(%R_(C))varying between 0.7%and 0.75%.展开更多
Objective:To evaluate the effectiveness of continuous nursing interventions in elderly patients with COPD in the stable phase and frailty.Methods:Sixty elderly patients with COPD in the stable phase and frailty,treate...Objective:To evaluate the effectiveness of continuous nursing interventions in elderly patients with COPD in the stable phase and frailty.Methods:Sixty elderly patients with COPD in the stable phase and frailty,treated between January 2024 and August 2024,were selected as the study subjects.Patients were randomly divided into two groups(30 each)using a drawing method.Patients who drew a black token were assigned to the intervention group and received continuous nursing interventions,while those who drew a red token were assigned to the nursing group and received standard nursing care.The quality of care between the groups was compared.Results:Pulmonary function indicators in the intervention group were significantly better than those in the nursing group(P<0.05).Immune function in the intervention group was also significantly higher than in the nursing group(P<0.05).Before the intervention,there was no significant difference in self-care ability between the two groups(P>0.05).However,post-intervention,both groups showed improved self-care abilities,with the intervention group scoring significantly higher than the nursing group(P<0.05).Similarly,before the intervention,there was no significant difference in quality-of-life scores(P>0.05),but post-intervention,both groups exhibited increased scores,with the intervention group outperforming the nursing group significantly(P<0.05).Conclusion:Continuous nursing interventions significantly improve outcomes in elderly patients with COPD in the stable phase and frailty,warranting broader implementation.展开更多
Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a...Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a typical semi-arid tributary in the upper Yellow River basin.This study aimed to investigate the spatiotemporal patterns and sources of NO_(3)^(-)in the basin using an integrated analysis of hydrochemistry,stable isotopes,and dissolved organic matter(DOM)fluorescence.Hydrochemical results revealed that the water quality exhibited distinct seasonal variations,influenced by the unique hydrological and climatic conditions of the upper Yellow River.The surface water showed elevated concentrations of NO_(3)^(-)with limited denitrification,and NO_(3)^(-)accumulation was driven by multiple sources.Based on the dual isotopes(δ^(15)N andδ^(18)O)and the MixSIAR model,it was quantitatively determined that allochthonous inputs—including chemical nitrogen fertilizer,soil nitrogen,domestic sewage,and manure—constituted the primary sources of NO_(3)^(-).DOM fluorescence analysis revealed active biological or microbial metabolic activities,while allochthonous DOM significantly contributed to NO_(3)^(-)contamination.Both the MixSIAR model and DOM fluorescence results confirmed that protein-like substances were mainly derived from domestic sewage,whereas humic-like substances originated from non-point source pollution in the study area.These findings demonstrate the feasibility of combining isotopic and DOM fluorescence approaches to trace NO_(3)^(-)sources in surface water.This integrated methodology can support the design of targeted zonal management strategies to protect the surface water environment and maintain sustainable socioeconomic systems in semi-arid regions.展开更多
In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes control...In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes controlling the groundwater chemistry is a major step in the sustainable management of the aquifers.A total of 29groundwater samples were collected and analysed.Ionic ratio graphs,multivariate statistical analysis,mineral saturation indices,stable isotopes,and geostatistics methods were used to examine the sources and the quality of the groundwater.The findings describe the water types in the district as Ca-Mg-HCO_(3)-Cl,Ca-Na-HCO_(3),Na-Ca-HCO_(3),Ca-Na-HCO_(3)-Cl,Na-Ca-HCO_(3)-Cl,mix water type,NaHCO_(3)-Cl,with possible evolution to Ca-Na-Cl-HCO_(3),and Na-Ca-Cl-HCO_(3).According to the IEWQI for drinking water,around 53.6% of the samples have good quality,whereas 10.7% have very low-quality groundwater.Only 3.45% of the samples are suitable to use for irrigation without treatment,whereas 41.4% are somewhat safe with minimal treatment.Water-rock interactions,including the dissolution and weathering of silicate minerals,cation exchange processes,and human activities like mining andquarrying,are some of the main factors influencing groundwater chemistry.Principal component analysis revealed that groundwater chemistry is influenced by a combination of natural and anthropogenic sources.The APCs-MLR receptor model quantifies the factors that play important roles in groundwater salinization,including mineral dissolution and weathering(19.4%),localised Cd(16%),Ni(14.6%),Pb(12.8%),and Fe(11.4%)contamination from urbanisation while unidentified sources of pollution account for about 26.0%.The stable isotopes revealed groundwater is of meteoric origin and water-rock interaction the major mechanism for groundwater mineralization.The results of this research highlight the need of implementing an integrated strategy for managing and accessing groundwater quality.展开更多
The aim of this study is to address the issues associated with traditional magnetorheological fluid(MRF)dampers,such as insufficient damping force after power failure and susceptibility to settlement.In order to achie...The aim of this study is to address the issues associated with traditional magnetorheological fluid(MRF)dampers,such as insufficient damping force after power failure and susceptibility to settlement.In order to achieve this,a bidirectional adjustable MRF damper was designed and developed.Magnetic field simulation analysis was conducted on the damper,along with simulation analysis on its dynamic characteristics.The dynamic characteristics were ultimately validated through experimental testing on the material testing machine,thereby corroborating the theoretical simulation results.Concurrently,this process generated valuable test data for subsequent implementation of the semi-active vibration control system.The simulation and test results demonstrate that the integrated permanent magnet effectively accomplishes bidirectional regulation.The magnetic induction intensity of the damping channel is 0.2 T in the absence of current,increases to 0.5 T when a maximum forward current of 4 A is applied,and becomes 0 T when a maximum reverse current of 3.8 A is applied.When the excitation amplitude is 8 mm and the frequency is 2 Hz,with the applied currents varying,the maximum damping force reaches 8 kN,while the minimum damping force measures at 511 N.Additionally,at zero current,the damping force stands at 2 kN,which aligns closely with simulation results.The present paper can serve as a valuable reference for the design and research of semi-active MRF dampers.展开更多
BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can pre...BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can predict all-cause mortality and major adverse CV events(MACE)in patients with coronary artery disease(CAD)and to determine the prognostic value of adiponectin in predicting all-cause mortality and MACE in patients with stable CAD.METHODS We conducted a systematic search on PubMed,Scopus,and Google Scholar to find relevant studies published through June 2023 evaluating the long-term prognostic role of adiponectin in patients with stable CAD.Using a random effects model with 95%CI,we estimated the odds ratio(OR)while assessing heterogeneity through I^(2)statistics.To ensure robustness,we performed a sensitivity analysis using the leave-one-out approach.RESULTS After screening,we included five prospective studies involving 3225 patients who were followed up for a median duration of 3.8 years.Within the study population,prevalent risk factors included hypertension,diabetes,hyperlipidemia,and smoking.The commonly prescribed medications were angiotensin-converting enzyme inhibitors,beta blockers,and statins.The combined adjusted OR for all-cause mortality was found to be 2.51(95%CI:1.36–4.62),showing heterogeneity(I^(2)=65.51%,P=0.03).On the other hand,the combined adjusted OR for MACE was determined to be 1.04(95%CI:1.02–1.06)with no significant heterogeneity observed(I^(2)=0%,P=0.68).Through a sensitivity analysis,it was discovered that none of the studies significantly impacted the overall results of the meta-analysis,thus indicating their robustness.CONCLUSION Higher levels of adiponectin were found to be associated with an increased risk of long-term mortality and MACE in patients with CAD,which highlights its potential as a cost-effective marker for risk assessment and guiding treatment strategies.Further research on the role of adiponectin could greatly influence decision-making and resource allocation in CV care.展开更多
Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macro...Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.展开更多
Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'...Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.展开更多
With the rapid development of generative artificial intelligence(GenAI),the task of story visualization,which transforms natural language narratives into coherent and consistent image sequences,has attracted growing r...With the rapid development of generative artificial intelligence(GenAI),the task of story visualization,which transforms natural language narratives into coherent and consistent image sequences,has attracted growing research attention.However,existing methods still face limitations in balancing multi-frame character consistency and generation efficiency,which restricts their feasibility for large-scale practical applications.To address this issue,this study proposes a modular cloud-based distributed system built on Stable Diffusion.By separating the character generation and story generation processes,and integratingmulti-feature control techniques,a cachingmechanism,and an asynchronous task queue architecture,the system enhances generation efficiency and scalability.The experimental design includes both automated and human evaluations of character consistency,performance testing,and multinode simulation.The results show that the proposed system outperforms the baseline model StoryGen in both CLIP-I and human evaluation metrics.In terms of performance,under the experimental environment of this study,dual-node deployment reduces average waiting time by approximately 19%,while the four-node simulation further reduces it by up to 65%.Overall,this study demonstrates the advantages of cloud-distributed GenAI in maintaining character consistency and reducing generation latency,highlighting its potential value inmulti-user collaborative story visualization applications.展开更多
The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recogni...The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility.展开更多
Objective:To evaluate the global,regional,and national burden and determinants of Acute Respiratory Infections(ARIs)among children and adolescents from 1990 to 2021.Methods:We analysed ARI mortality and disability-adj...Objective:To evaluate the global,regional,and national burden and determinants of Acute Respiratory Infections(ARIs)among children and adolescents from 1990 to 2021.Methods:We analysed ARI mortality and disability-adjusted life years(DALYs),stratified by age,sex,and economic development level based on data retrieved from the Global Burden of Disease study 2021.Decomposition and frontier analyses were employed to identify key drivers of burden variation and visualize potential reductions based on development levels.Results:Between 1990 and 2021,the global burden of ARIs showed a significant decline in both achievable age-standardized DALYs rate and age-standardized mortality rates(EAPC=-3.87 and-3.81,respectively).Different age groups and sex witnessed different levels of ARI burden,males experienced heavier burden than females and the 0-4 years-old group experienced heavier burden than other study age groups.Most of the 204 countries and territories experienced a downward trend of ARI burden,with slight increases observed only in Lesotho and Dominica.A negative correlation was found between the Socio-demographic Index and ARI burden.Decomposition analysis indicated that the significant decreases in deaths and DALYs were primarily driven by epidemiological changes.Conclusions:The global burden of ARIs among children and adolescents has declined over the past three decades,but substantial regional disparities persist.Targeted public health strategies are needed to address the continued ARI burden in high-risk regions and vulnerable age groups.展开更多
The detection and characterization of non-metallic inclusions are essential for clean steel production.Recently,imaging analysis combined with high-dimensional data processing of metallic materials using artificial in...The detection and characterization of non-metallic inclusions are essential for clean steel production.Recently,imaging analysis combined with high-dimensional data processing of metallic materials using artificial intelligence(AI)-based machine learning(ML)has developed rapidly.This technique has achieved impressive results in the field of inclusion classification in process metallurgy.The present study surveys the ML modeling of inclusion prediction in advanced steels,including the detection,classification,and feature prediction of inclusions in different steel grades.Studies on clean steel with different features based on data and image analysis via ML are summarized.Regarding the data analysis,the inclusion prediction methodology based on ML establishes a connection between the experimental parameters and inclusion characteristics and analyzes the importance of the experimental parameters.Regarding the image analysis,the focus is placed on the classification of different types of inclusions via deep learning,in comparison with data analysis.Finally,further development of inclusion analyses using ML-based methods is recommended.This work paves the way for the application of AIbased methodologies for ultraclean-steel studies from a sustainable metallurgy perspective.展开更多
Red-fleshed fruits are valued for their vibrant color and high anthocyanin content.Pre-harvest fruit bagging enhances fruit peel pigmentation,but its effect on flesh coloration remains poorly characterized.This study ...Red-fleshed fruits are valued for their vibrant color and high anthocyanin content.Pre-harvest fruit bagging enhances fruit peel pigmentation,but its effect on flesh coloration remains poorly characterized.This study revealed that removing bags from‘Gengcunyangtao’red-fleshed peach fruits triggers the rapid and uniform accumulation of anthocyanins in the flesh,resulting in anthocyanin levels that exceed those in unbagged fruits.The exposure to light after bag removal triggered significant increases in anthocyanin levels within 24 h.This was accompanied by the rapid upregulation of light-responsive and flavonoid biosynthetic gene expression levels within 6 h.A metabolomic analysis indicated that anthocyanin precursors,especially p-coumaric acid,accumulated before bag removal,thereby increasing substrate availability for rapid anthocyanin synthesis.On the basis of a weighted gene co-expression network analysis,MYB transcription factors,anthocyanin transporters,glutathione S-transferase,and multidrug and toxic compound extrusion(MATE)were identified as key regulators that coordinate precursor storage along with light-induced transcriptional activation.Notably,PpMYB4 binds to the promoter of PpGSTF14 and activates its expression,thereby promoting anthocyanin accumulation.The study findings elucidated the temporal coordination of metabolic priming and light-responsive transcriptional regulation driving rapid anthocyanin biosynthesis,with possible implications for improving peach fruit flesh coloration.展开更多
Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations...Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations,and functional implications of RIPK family members across various cancers.Methods:We collected multi-omics data from The Cancer Genome Atlas and other public databases,including gene expression,copy number variation(CNV),mutation,methylation,tumor mutation burden(TMB),and microsatellite instability(MSI).Differential expression and survival analyses were performed using DESeq2 and Cox proportional hazards models.CNV and mutation data were analyzed with GISTIC2 and Mutect2,and methylation data with the ChAMP package.Correlations with TMB and MSI were assessed using Pearson coefficients,and gene set enrichment analysis was conducted with the MSigDB Hallmark gene sets.Results:RIPK family members show significant differential expression in various cancers,with RIPK1 and RIPK4 frequently altered.Survival analysis reveals heterogeneous impacts on overall survival.CNV and mutation analyses identify high alteration frequencies for RIPK2 and RIPK7,affecting gene expression.RIPK1 and RIPK7 are hypermethylated in several cancers,inversely correlating with RIPK3 expression.RIPK1,RIPK2,RIPK5,RIPK6,and RIPK7 correlate positively with TMB,while RIPK3 shows negative correlations in some cancers.MSI analysis indicates associations with DNA mismatch repair.G ene set enrichment analysis highlights immune-related pathway enrichment for RIPK1,RIPK2,RIPK3,and RIPK6,and cell proliferation and DNA repair pathways for RIPK4 and RIPK5.RIPK family members showed heterogeneous alterations across cancers:for example,RIPK7 was mutated in up to~15%of u terine c orpus e ndometrial c arcinoma and l ung s quamous c ell c arcinoma cases,and RIPK1 and RIPK7 exhibited frequent promoter hypermethylation in multiple tumor types.Several genes displayed context-dependent associations with overall survival and with TMB/MSI.Conclusion:This pan-cancer analysis of the RIPK family reveals their diverse roles and potential as biomarkers and therapeutic targets.The findings emphasize the importance of RIPK genes in tumorigenesis and suggest context-dependent functions across cancer types.Further studies are needed to explore their mechanisms in cancer development and clinical applications.展开更多
[Objective]This study aims to investigate the multi-body hydrodynamic interaction mechanisms during offshore lifting operations of aquaculture net cages in wind-fishery integration systems.By integrating numerical sim...[Objective]This study aims to investigate the multi-body hydrodynamic interaction mechanisms during offshore lifting operations of aquaculture net cages in wind-fishery integration systems.By integrating numerical simulations and dynamic analysis methods,this study systematically investigates the coupled dynamic response characteristics during the cage-carrier vessel separation process to reveal its dynamic evolution patterns and key influence mechanisms.[Method]Based on potential flow theory,a fully coupled dynamic analysis model of crane vessel-net cage-semi-submersible barge was established for a marine ranch project in Guangdong.The complete lifting process was dynamically simulated using SESAM software.Five typical operating sea states were configured to investigate the influence of wave parameters on the system's motion response under combined wave-current-wind actions.[Result]The results demonstrate that wave period dominates the system stability.Under short-period conditions,the system maintains stable motion with relatively small horizontal relative displacements,while long-period conditions excite low-frequency resonance,leading to significant slow-drift motions.Vertical response analysis reveals that long-period waves cause severe relative displacement fluctuations between the cage and semi-submersible vessel,with actual displacement amplitudes doubling the preset safety target of 2.045 m.Quantitative analysis further indicates that when significant wave height increases from 1.0 m to 1.5 m,the actual displacement amplitude increases by approximately 20%relative to the target displacement of 2.045 m,demonstrating that its influence is significantly weaker than the displacement variations induced by wave period changes.The complete dynamic simulation successfully captures the continuous dynamic response characteristics during the lifting process.[Conclusion]This research clarifies the influence mechanisms of wave parameters on the cage lifting process,identifying wave period as the crucial factor for operational safety.An operation window assessment method incorporating multi-body coupling effects is established,proposing a safety criterion with peak period not exceeding six seconds as the core requirement.The findings provide theoretical foundation for safe installation of marine ranch net cages and offer valuable references for similar offshore lifting operations.展开更多
The sulfation and decomposition process has proven effective in selectively extracting lithium from lepidolite.It is essential to clarify the thermochemical behavior and kinetic parameters of decomposition reactions.A...The sulfation and decomposition process has proven effective in selectively extracting lithium from lepidolite.It is essential to clarify the thermochemical behavior and kinetic parameters of decomposition reactions.Accordingly,comprehensive kinetic study by employing thermalgravimetric analysis at various heating rates was presented in this paper.Two main weight loss regions were observed during heating.The initial region corresponded to the dehydration of crystal water,whereas the subsequent region with overlapping peaks involved complex decomposition reactions.The overlapping peaks were separated into two individual reaction peaks and the activation energy of each peak was calculated using isoconversional kinetics methods.The activation energy of peak 1 exhibited a continual increase as the reaction conversion progressed,while that of peak 2 steadily decreased.The optimal kinetic models,identified as belonging to the random nucleation and subsequent growth category,provided valuable insights into the mechanism of the decomposition reactions.Furthermore,the adjustment factor was introduced to reconstruct the kinetic mechanism models,and the reconstructed models described the kinetic mechanism model more accurately for the decomposition reactions.This study enhanced the understanding of the thermochemical behavior and kinetic parameters of the lepidolite sulfation product decomposition reactions,further providing theoretical basis for promoting the selective extraction of lithium.展开更多
Fault features in mechanical systems often manifest as transient impulses,which can be effectively analyzed using time-frequency analysis(TFA)methods.Recently,a new TFA technique known as the time-reassigned multi-syn...Fault features in mechanical systems often manifest as transient impulses,which can be effectively analyzed using time-frequency analysis(TFA)methods.Recently,a new TFA technique known as the time-reassigned multi-synchrosqueezing transform(TMssT)was proposed to capture these transient impulses for fault diagnosis.However,the TMSST,which is based on the short-time Fourier transform(STFT),suffers from unclear high-frequency re-presentations owing to the fixed sliding window used in the STFT.To address this limitation,the current study combined TMSST with the S-transform and a local maximum method to enhance the time-frequency representation for improved signal analysis.Furthermore,an extractive reconstruction algorithm that binds the maximum value of the spectral envelope is proposed for spectral decomposition.To validate the proposed technique,a simulated noise-added signal and four experimental bearing defect datasets were used.The results demonstrate that the proposed technique can effectively and accurately extract fault features from bearing signals regardless of whether the bearings operate under constant or varying speed conditions.This study offers a novel and efficient approach for fault diagnosis in mechanical systems with complex dynamic behaviors.展开更多
With the rapid proliferation of electric vehicles,their charging loads pose new challenges to power grid stability and operational efficiency.To address this,this study employs a Monte Carlo simulation model to analyz...With the rapid proliferation of electric vehicles,their charging loads pose new challenges to power grid stability and operational efficiency.To address this,this study employs a Monte Carlo simulation model to analyze the charging load characteristics of six battery electric vehicle categories in Hebei Province,leveraging multi-source probabilistic distribution data under typical operational scenarios.The findings reveal that electric vehicle charging loads are primarily concentrated during midday and nighttime periods,with significant load fluctuations exerting substantial pressure on the grid.In response,this paper proposes strategic interventions including optimized charging infrastructure planning,time-of-use electricity pricing mechanisms,and smart charging technologies to balance grid loads.The results provide a theoretical foundation for electric vehicle load forecasting,smart grid dispatching,and vehicle-grid integration,thereby enhancing grid operational efficiency and sustainability.展开更多
基金supported by the Scientific Research Foundation of Hainan Tropical Ocean University(No.RHDRC202312)the Major Science and Technology Project of Yazhou Bay Innovation Institute of Hainan Tropical Ocean University:Research on Hainan Coastal Marine Ecological Environment and Carbon Sink(No.2022CXYZD002).
文摘Fish scaleδ^(15)N(δ^(15)N_(sca))records trophic information of the whole life history for marine fishes.Data from gut content and values calculated from white muscle tissue(WMT)are insufficient for assessing trophic level(TL)of marine fishes as they only provide the information of several months or even several days.Stable isotope analysis(SIA)of nitrogen was carried out in fish scales and WMT of 16 species from a total of 28 individuals captured from the Yellow Sea and northern East China Sea in October and November of 2019,aiming at discovering the correlation betweenδ^(15)N_(sca) and fish WMTδ^(15)N(δ^(15)NWMT),so as to useδ^(15)N_(sca) for the characterization of TLs of marine fish.Scales were analyzed without prior carbonate dissolution as a pre-processing step.TL calculated fromδ^(15)N_(sca) was compared to traditional TL formula calculated withδ^(15)N_(WMT) to estimate the accuracy.We found that there was a highly significant and positive correlation(r=0.830,P<0.001)betweenδ^(15)N_(sca) andδ^(15)N_(WMT).TL calculation results using fish scale(TL_(sca))and WMT(TL_(WMT))showed congruence(r=0.773,P<0.001),and the regression coefficient betweenδ^(15)N_(sca) andδ^(15)NWMT was used for calculating TLsca.The conversion was conducted for the purpose of comparison with previousδ^(15)N_(WMT)-based calculation results of TL.TLsca calculation results were 81.25%within the TL range of±10%calculated withδ^(15)NWMT.The findings in the present study showed thatδ^(15)N_(sca) is a viable technique for the characterization of the TL of marine fish and can help to predict food web and support the study of marine ecosystem.
文摘Biomarker and stable carbon isotope analyses are presented for the Garau Formation of the Cretaceous Age,an important source rock in western Iran,to reveal its potential as an oilprone source rock.The C_(28)/C_(29)sterane ratio value range(0.72 to 0.83)of bitumen samples from the formation suggests that they were likely formed during phytoplankton blooms.Sterane,hopane,and isoprenoid/n-alkane ratios indicate that the formation's organic matter predominantly consists of algae,and bacteria,accompanied by some reworked material derived from higher plants.Due to the predominance of anoxic conditions and the actions of sulfate-reducing bacteria,the bitumen present is enriched with sulfur compounds.The percentages of saturates,aromatics,and nitrogen-sulfuroxygen(NSO)fractions in the bitumen samples classify them as naphthenic oils.Isotope analysis reveals that biodegradation and water-washing have reduced the concentrations of some volatile saturates and low molecular weight aromatics in the bitumen samples.These actions have resulted in distinctiveδ^(13)C values for the formation's kerogen and bitumen fractions.The formation's organic matter has been subjected to high-temperature thermal regimes and has entered the oil-generation window at the sampled localities,with vitrinite reflectance(%R_(C))varying between 0.7%and 0.75%.
文摘Objective:To evaluate the effectiveness of continuous nursing interventions in elderly patients with COPD in the stable phase and frailty.Methods:Sixty elderly patients with COPD in the stable phase and frailty,treated between January 2024 and August 2024,were selected as the study subjects.Patients were randomly divided into two groups(30 each)using a drawing method.Patients who drew a black token were assigned to the intervention group and received continuous nursing interventions,while those who drew a red token were assigned to the nursing group and received standard nursing care.The quality of care between the groups was compared.Results:Pulmonary function indicators in the intervention group were significantly better than those in the nursing group(P<0.05).Immune function in the intervention group was also significantly higher than in the nursing group(P<0.05).Before the intervention,there was no significant difference in self-care ability between the two groups(P>0.05).However,post-intervention,both groups showed improved self-care abilities,with the intervention group scoring significantly higher than the nursing group(P<0.05).Similarly,before the intervention,there was no significant difference in quality-of-life scores(P>0.05),but post-intervention,both groups exhibited increased scores,with the intervention group outperforming the nursing group significantly(P<0.05).Conclusion:Continuous nursing interventions significantly improve outcomes in elderly patients with COPD in the stable phase and frailty,warranting broader implementation.
基金The Basic Special Business Fund for Research and Development for the Central Level Scientific Research Institutes,Nanjing Institute of Environmental Sciences,MEE,Grant/Award Number:GYZX240103The Innovative Team Project of Nanjing Institute of Environmental Sciences,MEE,Grant/Award Number:GYZX200101Geological Prospecting Funds Program of Zhejiang Province,China,Grant/Award Number:No.2023021。
文摘Identifying the sources and characteristics of water pollution is essential for the protection and management of water environments.Nitrate(NO_(3)^(-))is a key pollutant affecting water quality in the Qingshui River,a typical semi-arid tributary in the upper Yellow River basin.This study aimed to investigate the spatiotemporal patterns and sources of NO_(3)^(-)in the basin using an integrated analysis of hydrochemistry,stable isotopes,and dissolved organic matter(DOM)fluorescence.Hydrochemical results revealed that the water quality exhibited distinct seasonal variations,influenced by the unique hydrological and climatic conditions of the upper Yellow River.The surface water showed elevated concentrations of NO_(3)^(-)with limited denitrification,and NO_(3)^(-)accumulation was driven by multiple sources.Based on the dual isotopes(δ^(15)N andδ^(18)O)and the MixSIAR model,it was quantitatively determined that allochthonous inputs—including chemical nitrogen fertilizer,soil nitrogen,domestic sewage,and manure—constituted the primary sources of NO_(3)^(-).DOM fluorescence analysis revealed active biological or microbial metabolic activities,while allochthonous DOM significantly contributed to NO_(3)^(-)contamination.Both the MixSIAR model and DOM fluorescence results confirmed that protein-like substances were mainly derived from domestic sewage,whereas humic-like substances originated from non-point source pollution in the study area.These findings demonstrate the feasibility of combining isotopic and DOM fluorescence approaches to trace NO_(3)^(-)sources in surface water.This integrated methodology can support the design of targeted zonal management strategies to protect the surface water environment and maintain sustainable socioeconomic systems in semi-arid regions.
文摘In many places across the globe,including the Wassa District of Ghana,groundwater provides a significant supply of water for various purposes.Understanding the groundwater origin and hydrogeochemical processes controlling the groundwater chemistry is a major step in the sustainable management of the aquifers.A total of 29groundwater samples were collected and analysed.Ionic ratio graphs,multivariate statistical analysis,mineral saturation indices,stable isotopes,and geostatistics methods were used to examine the sources and the quality of the groundwater.The findings describe the water types in the district as Ca-Mg-HCO_(3)-Cl,Ca-Na-HCO_(3),Na-Ca-HCO_(3),Ca-Na-HCO_(3)-Cl,Na-Ca-HCO_(3)-Cl,mix water type,NaHCO_(3)-Cl,with possible evolution to Ca-Na-Cl-HCO_(3),and Na-Ca-Cl-HCO_(3).According to the IEWQI for drinking water,around 53.6% of the samples have good quality,whereas 10.7% have very low-quality groundwater.Only 3.45% of the samples are suitable to use for irrigation without treatment,whereas 41.4% are somewhat safe with minimal treatment.Water-rock interactions,including the dissolution and weathering of silicate minerals,cation exchange processes,and human activities like mining andquarrying,are some of the main factors influencing groundwater chemistry.Principal component analysis revealed that groundwater chemistry is influenced by a combination of natural and anthropogenic sources.The APCs-MLR receptor model quantifies the factors that play important roles in groundwater salinization,including mineral dissolution and weathering(19.4%),localised Cd(16%),Ni(14.6%),Pb(12.8%),and Fe(11.4%)contamination from urbanisation while unidentified sources of pollution account for about 26.0%.The stable isotopes revealed groundwater is of meteoric origin and water-rock interaction the major mechanism for groundwater mineralization.The results of this research highlight the need of implementing an integrated strategy for managing and accessing groundwater quality.
文摘The aim of this study is to address the issues associated with traditional magnetorheological fluid(MRF)dampers,such as insufficient damping force after power failure and susceptibility to settlement.In order to achieve this,a bidirectional adjustable MRF damper was designed and developed.Magnetic field simulation analysis was conducted on the damper,along with simulation analysis on its dynamic characteristics.The dynamic characteristics were ultimately validated through experimental testing on the material testing machine,thereby corroborating the theoretical simulation results.Concurrently,this process generated valuable test data for subsequent implementation of the semi-active vibration control system.The simulation and test results demonstrate that the integrated permanent magnet effectively accomplishes bidirectional regulation.The magnetic induction intensity of the damping channel is 0.2 T in the absence of current,increases to 0.5 T when a maximum forward current of 4 A is applied,and becomes 0 T when a maximum reverse current of 3.8 A is applied.When the excitation amplitude is 8 mm and the frequency is 2 Hz,with the applied currents varying,the maximum damping force reaches 8 kN,while the minimum damping force measures at 511 N.Additionally,at zero current,the damping force stands at 2 kN,which aligns closely with simulation results.The present paper can serve as a valuable reference for the design and research of semi-active MRF dampers.
文摘BACKGROUND The persistent burden of cardiovascular(CV)disease in the United States requires innovative and cost-effective prognostic markers that can be relied upon.AIM To provide insights into how adiponectin can predict all-cause mortality and major adverse CV events(MACE)in patients with coronary artery disease(CAD)and to determine the prognostic value of adiponectin in predicting all-cause mortality and MACE in patients with stable CAD.METHODS We conducted a systematic search on PubMed,Scopus,and Google Scholar to find relevant studies published through June 2023 evaluating the long-term prognostic role of adiponectin in patients with stable CAD.Using a random effects model with 95%CI,we estimated the odds ratio(OR)while assessing heterogeneity through I^(2)statistics.To ensure robustness,we performed a sensitivity analysis using the leave-one-out approach.RESULTS After screening,we included five prospective studies involving 3225 patients who were followed up for a median duration of 3.8 years.Within the study population,prevalent risk factors included hypertension,diabetes,hyperlipidemia,and smoking.The commonly prescribed medications were angiotensin-converting enzyme inhibitors,beta blockers,and statins.The combined adjusted OR for all-cause mortality was found to be 2.51(95%CI:1.36–4.62),showing heterogeneity(I^(2)=65.51%,P=0.03).On the other hand,the combined adjusted OR for MACE was determined to be 1.04(95%CI:1.02–1.06)with no significant heterogeneity observed(I^(2)=0%,P=0.68).Through a sensitivity analysis,it was discovered that none of the studies significantly impacted the overall results of the meta-analysis,thus indicating their robustness.CONCLUSION Higher levels of adiponectin were found to be associated with an increased risk of long-term mortality and MACE in patients with CAD,which highlights its potential as a cost-effective marker for risk assessment and guiding treatment strategies.Further research on the role of adiponectin could greatly influence decision-making and resource allocation in CV care.
基金supported by Qingdao Key Medical and Health Discipline ProjectThe Intramural Research Program of the Affiliated Hospital of Qingdao University,No. 4910Qingdao West Coast New Area Science and Technology Project,No. 2020-55 (all to SW)。
文摘Border-associated macrophages are located at the interface between the brain and the periphery, including the perivascular spaces, choroid plexus, and meninges. Until recently, the functions of border-associated macrophages have been poorly understood and largely overlooked. However, a recent study reported that border-associated macrophages participate in stroke-induced inflammation, although many details and the underlying mechanisms remain unclear. In this study, we performed a comprehensive single-cell analysis of mouse border-associated macrophages using sequencing data obtained from the Gene Expression Omnibus(GEO) database(GSE174574 and GSE225948). Differentially expressed genes were identified, and enrichment analysis was performed to identify the transcription profile of border-associated macrophages. CellChat analysis was conducted to determine the cell communication network of border-associated macrophages. Transcription factors were predicted using the ‘pySCENIC' tool. We found that, in response to hypoxia, borderassociated macrophages underwent dynamic transcriptional changes and participated in the regulation of inflammatory-related pathways. Notably, the tumor necrosis factor pathway was activated by border-associated macrophages following ischemic stroke. The pySCENIC analysis indicated that the activity of signal transducer and activator of transcription 3(Stat3) was obviously upregulated in stroke, suggesting that Stat3 inhibition may be a promising strategy for treating border-associated macrophages-induced neuroinflammation. Finally, we constructed an animal model to investigate the effects of border-associated macrophages depletion following a stroke. Treatment with liposomes containing clodronate significantly reduced infarct volume in the animals and improved neurological scores compared with untreated animals. Taken together, our results demonstrate comprehensive changes in border-associated macrophages following a stroke, providing a theoretical basis for targeting border-associated macrophages-induced neuroinflammation in stroke treatment.
文摘Six new lanthanide complexes:[Ln(3,4-DEOBA)3(4,4'-DM-2,2'-bipy)]2·2C_(2)H_(5)OH,[Ln=Dy(1),Eu(2),Tb(3),Sm(4),Ho(5),Gd(6);3,4-DEOBA-=3,4-diethoxybenzoate,4,4'-DM-2,2'-bipy=4,4'-dimethyl-2,2'-bipyridine]were successfully synthesized by the volatilization of the solution at room temperature.The crystal structures of six complexes were determined by single-crystal X-ray diffraction technology.The results showed that the complexes all have a binuclear structure,and the structures contain free ethanol molecules.Moreover,the coordination number of the central metal of each structural unit is eight.Adjacent structural units interact with each other through hydrogen bonds and further expand to form 1D chain-like and 2D planar structures.After conducting a systematic study on the luminescence properties of complexes 1-4,their emission and excitation spectra were obtained.Experimental results indicated that the fluorescence lifetimes of complexes 2 and 3 were 0.807 and 0.845 ms,respectively.The emission spectral data of complexes 1-4 were imported into the CIE chromaticity coordinate system,and their corre sponding luminescent regions cover the yellow light,red light,green light,and orange-red light bands,respectively.Within the temperature range of 299.15-1300 K,the thermal decomposition processes of the six complexes were comprehensively analyzed by using TG-DSC/FTIR/MS technology.The hypothesis of the gradual loss of ligand groups during the decomposition process was verified by detecting the escaped gas,3D infrared spectroscopy,and ion fragment information detected by mass spectrometry.The specific decomposition path is as follows:firstly,free ethanol molecules and neutral ligands are removed,and finally,acidic ligands are released;the final product is the corresponding metal oxide.CCDC:2430420,1;2430422,2;2430419,3;2430424,4;2430421,5;2430423,6.
文摘With the rapid development of generative artificial intelligence(GenAI),the task of story visualization,which transforms natural language narratives into coherent and consistent image sequences,has attracted growing research attention.However,existing methods still face limitations in balancing multi-frame character consistency and generation efficiency,which restricts their feasibility for large-scale practical applications.To address this issue,this study proposes a modular cloud-based distributed system built on Stable Diffusion.By separating the character generation and story generation processes,and integratingmulti-feature control techniques,a cachingmechanism,and an asynchronous task queue architecture,the system enhances generation efficiency and scalability.The experimental design includes both automated and human evaluations of character consistency,performance testing,and multinode simulation.The results show that the proposed system outperforms the baseline model StoryGen in both CLIP-I and human evaluation metrics.In terms of performance,under the experimental environment of this study,dual-node deployment reduces average waiting time by approximately 19%,while the four-node simulation further reduces it by up to 65%.Overall,this study demonstrates the advantages of cloud-distributed GenAI in maintaining character consistency and reducing generation latency,highlighting its potential value inmulti-user collaborative story visualization applications.
文摘The Savitzky-Golay(SG)filter,which employs polynomial least-squares approximations to smooth data and estimate derivatives,is widely used for processing noisy data.However,noise suppression by the SG filter is recognized to be limited at data boundaries and high frequencies,which can significantly reduce the signal-to-noise ratio(SNR).To solve this problem,a novel method synergistically integrating Principal Component Analysis(PCA)with SG filtering is proposed in this paper.This approach avoids the is-sue of excessive smoothing associated with larger window sizes.The proposed PCA-SG filtering algorithm was applied to a CO gas sensing system based on Cavity Ring-Down Spectroscopy(CRDS).The perform-ance of the PCA-SG filtering algorithm is demonstrated through comparison with Moving Average Filtering(MAF),Wavelet Transformation(WT),Kalman Filtering(KF),and the SG filter.The results demonstrate that the proposed algorithm exhibits superior noise reduction capabilities compared to the other algorithms evaluated.The SNR of the ring-down signal was improved from 11.8612 dB to 29.0913 dB,and the stand-ard deviation of the extracted ring-down time constant was reduced from 0.037μs to 0.018μs.These results confirm that the proposed PCA-SG filtering algorithm effectively improves the smoothness of the ring-down curve data,demonstrating its feasibility.
文摘Objective:To evaluate the global,regional,and national burden and determinants of Acute Respiratory Infections(ARIs)among children and adolescents from 1990 to 2021.Methods:We analysed ARI mortality and disability-adjusted life years(DALYs),stratified by age,sex,and economic development level based on data retrieved from the Global Burden of Disease study 2021.Decomposition and frontier analyses were employed to identify key drivers of burden variation and visualize potential reductions based on development levels.Results:Between 1990 and 2021,the global burden of ARIs showed a significant decline in both achievable age-standardized DALYs rate and age-standardized mortality rates(EAPC=-3.87 and-3.81,respectively).Different age groups and sex witnessed different levels of ARI burden,males experienced heavier burden than females and the 0-4 years-old group experienced heavier burden than other study age groups.Most of the 204 countries and territories experienced a downward trend of ARI burden,with slight increases observed only in Lesotho and Dominica.A negative correlation was found between the Socio-demographic Index and ARI burden.Decomposition analysis indicated that the significant decreases in deaths and DALYs were primarily driven by epidemiological changes.Conclusions:The global burden of ARIs among children and adolescents has declined over the past three decades,but substantial regional disparities persist.Targeted public health strategies are needed to address the continued ARI burden in high-risk regions and vulnerable age groups.
基金support from the National Key Research and Development Program of China(No.2024YFB3713705)is acknowledgedWangzhong Mu would like to acknowledge the Strategic Mobility,Sweden(SSF,No.SM22-0039)+1 种基金the Swedish Foundation for International Cooperation in Research and Higher Education(STINT,No.IB2022-9228)the Jernkontoret(Sweden)for supporting this clean steel research.Gonghao Lian would like to acknowledge China Scholarship Council(CSC,No.202306080032).
文摘The detection and characterization of non-metallic inclusions are essential for clean steel production.Recently,imaging analysis combined with high-dimensional data processing of metallic materials using artificial intelligence(AI)-based machine learning(ML)has developed rapidly.This technique has achieved impressive results in the field of inclusion classification in process metallurgy.The present study surveys the ML modeling of inclusion prediction in advanced steels,including the detection,classification,and feature prediction of inclusions in different steel grades.Studies on clean steel with different features based on data and image analysis via ML are summarized.Regarding the data analysis,the inclusion prediction methodology based on ML establishes a connection between the experimental parameters and inclusion characteristics and analyzes the importance of the experimental parameters.Regarding the image analysis,the focus is placed on the classification of different types of inclusions via deep learning,in comparison with data analysis.Finally,further development of inclusion analyses using ML-based methods is recommended.This work paves the way for the application of AIbased methodologies for ultraclean-steel studies from a sustainable metallurgy perspective.
基金supported by the Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(Grant No.2021C12066-4)Huzhou Agricultural Science and Technology Innovation Team Project(Grant No.2022HN01).
文摘Red-fleshed fruits are valued for their vibrant color and high anthocyanin content.Pre-harvest fruit bagging enhances fruit peel pigmentation,but its effect on flesh coloration remains poorly characterized.This study revealed that removing bags from‘Gengcunyangtao’red-fleshed peach fruits triggers the rapid and uniform accumulation of anthocyanins in the flesh,resulting in anthocyanin levels that exceed those in unbagged fruits.The exposure to light after bag removal triggered significant increases in anthocyanin levels within 24 h.This was accompanied by the rapid upregulation of light-responsive and flavonoid biosynthetic gene expression levels within 6 h.A metabolomic analysis indicated that anthocyanin precursors,especially p-coumaric acid,accumulated before bag removal,thereby increasing substrate availability for rapid anthocyanin synthesis.On the basis of a weighted gene co-expression network analysis,MYB transcription factors,anthocyanin transporters,glutathione S-transferase,and multidrug and toxic compound extrusion(MATE)were identified as key regulators that coordinate precursor storage along with light-induced transcriptional activation.Notably,PpMYB4 binds to the promoter of PpGSTF14 and activates its expression,thereby promoting anthocyanin accumulation.The study findings elucidated the temporal coordination of metabolic priming and light-responsive transcriptional regulation driving rapid anthocyanin biosynthesis,with possible implications for improving peach fruit flesh coloration.
基金supported by grants from the Tianjin Health Technology Project(Grant no.2022QN106).
文摘Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations,and functional implications of RIPK family members across various cancers.Methods:We collected multi-omics data from The Cancer Genome Atlas and other public databases,including gene expression,copy number variation(CNV),mutation,methylation,tumor mutation burden(TMB),and microsatellite instability(MSI).Differential expression and survival analyses were performed using DESeq2 and Cox proportional hazards models.CNV and mutation data were analyzed with GISTIC2 and Mutect2,and methylation data with the ChAMP package.Correlations with TMB and MSI were assessed using Pearson coefficients,and gene set enrichment analysis was conducted with the MSigDB Hallmark gene sets.Results:RIPK family members show significant differential expression in various cancers,with RIPK1 and RIPK4 frequently altered.Survival analysis reveals heterogeneous impacts on overall survival.CNV and mutation analyses identify high alteration frequencies for RIPK2 and RIPK7,affecting gene expression.RIPK1 and RIPK7 are hypermethylated in several cancers,inversely correlating with RIPK3 expression.RIPK1,RIPK2,RIPK5,RIPK6,and RIPK7 correlate positively with TMB,while RIPK3 shows negative correlations in some cancers.MSI analysis indicates associations with DNA mismatch repair.G ene set enrichment analysis highlights immune-related pathway enrichment for RIPK1,RIPK2,RIPK3,and RIPK6,and cell proliferation and DNA repair pathways for RIPK4 and RIPK5.RIPK family members showed heterogeneous alterations across cancers:for example,RIPK7 was mutated in up to~15%of u terine c orpus e ndometrial c arcinoma and l ung s quamous c ell c arcinoma cases,and RIPK1 and RIPK7 exhibited frequent promoter hypermethylation in multiple tumor types.Several genes displayed context-dependent associations with overall survival and with TMB/MSI.Conclusion:This pan-cancer analysis of the RIPK family reveals their diverse roles and potential as biomarkers and therapeutic targets.The findings emphasize the importance of RIPK genes in tumorigenesis and suggest context-dependent functions across cancer types.Further studies are needed to explore their mechanisms in cancer development and clinical applications.
文摘[Objective]This study aims to investigate the multi-body hydrodynamic interaction mechanisms during offshore lifting operations of aquaculture net cages in wind-fishery integration systems.By integrating numerical simulations and dynamic analysis methods,this study systematically investigates the coupled dynamic response characteristics during the cage-carrier vessel separation process to reveal its dynamic evolution patterns and key influence mechanisms.[Method]Based on potential flow theory,a fully coupled dynamic analysis model of crane vessel-net cage-semi-submersible barge was established for a marine ranch project in Guangdong.The complete lifting process was dynamically simulated using SESAM software.Five typical operating sea states were configured to investigate the influence of wave parameters on the system's motion response under combined wave-current-wind actions.[Result]The results demonstrate that wave period dominates the system stability.Under short-period conditions,the system maintains stable motion with relatively small horizontal relative displacements,while long-period conditions excite low-frequency resonance,leading to significant slow-drift motions.Vertical response analysis reveals that long-period waves cause severe relative displacement fluctuations between the cage and semi-submersible vessel,with actual displacement amplitudes doubling the preset safety target of 2.045 m.Quantitative analysis further indicates that when significant wave height increases from 1.0 m to 1.5 m,the actual displacement amplitude increases by approximately 20%relative to the target displacement of 2.045 m,demonstrating that its influence is significantly weaker than the displacement variations induced by wave period changes.The complete dynamic simulation successfully captures the continuous dynamic response characteristics during the lifting process.[Conclusion]This research clarifies the influence mechanisms of wave parameters on the cage lifting process,identifying wave period as the crucial factor for operational safety.An operation window assessment method incorporating multi-body coupling effects is established,proposing a safety criterion with peak period not exceeding six seconds as the core requirement.The findings provide theoretical foundation for safe installation of marine ranch net cages and offer valuable references for similar offshore lifting operations.
基金financially supported by the National Natural Science Foundation of China(Nos.52034002 and U2202254)the Fundamental Research Funds for the Central Universities,China(No.FRF-TT-19-001)。
文摘The sulfation and decomposition process has proven effective in selectively extracting lithium from lepidolite.It is essential to clarify the thermochemical behavior and kinetic parameters of decomposition reactions.Accordingly,comprehensive kinetic study by employing thermalgravimetric analysis at various heating rates was presented in this paper.Two main weight loss regions were observed during heating.The initial region corresponded to the dehydration of crystal water,whereas the subsequent region with overlapping peaks involved complex decomposition reactions.The overlapping peaks were separated into two individual reaction peaks and the activation energy of each peak was calculated using isoconversional kinetics methods.The activation energy of peak 1 exhibited a continual increase as the reaction conversion progressed,while that of peak 2 steadily decreased.The optimal kinetic models,identified as belonging to the random nucleation and subsequent growth category,provided valuable insights into the mechanism of the decomposition reactions.Furthermore,the adjustment factor was introduced to reconstruct the kinetic mechanism models,and the reconstructed models described the kinetic mechanism model more accurately for the decomposition reactions.This study enhanced the understanding of the thermochemical behavior and kinetic parameters of the lepidolite sulfation product decomposition reactions,further providing theoretical basis for promoting the selective extraction of lithium.
基金Supported by National Natural Science Foundation of China(Grant No.62271230)Shandong Provincial Central Guidance on Local Science and Technology Development Fund(Grant No.YDZX2022178).
文摘Fault features in mechanical systems often manifest as transient impulses,which can be effectively analyzed using time-frequency analysis(TFA)methods.Recently,a new TFA technique known as the time-reassigned multi-synchrosqueezing transform(TMssT)was proposed to capture these transient impulses for fault diagnosis.However,the TMSST,which is based on the short-time Fourier transform(STFT),suffers from unclear high-frequency re-presentations owing to the fixed sliding window used in the STFT.To address this limitation,the current study combined TMSST with the S-transform and a local maximum method to enhance the time-frequency representation for improved signal analysis.Furthermore,an extractive reconstruction algorithm that binds the maximum value of the spectral envelope is proposed for spectral decomposition.To validate the proposed technique,a simulated noise-added signal and four experimental bearing defect datasets were used.The results demonstrate that the proposed technique can effectively and accurately extract fault features from bearing signals regardless of whether the bearings operate under constant or varying speed conditions.This study offers a novel and efficient approach for fault diagnosis in mechanical systems with complex dynamic behaviors.
基金funded by Humanities and Social Sciences of Ministry of Education Planning Fund of China,grant number 21YJA790009National Natural Science Foundation of China,grant number 72140001.
文摘With the rapid proliferation of electric vehicles,their charging loads pose new challenges to power grid stability and operational efficiency.To address this,this study employs a Monte Carlo simulation model to analyze the charging load characteristics of six battery electric vehicle categories in Hebei Province,leveraging multi-source probabilistic distribution data under typical operational scenarios.The findings reveal that electric vehicle charging loads are primarily concentrated during midday and nighttime periods,with significant load fluctuations exerting substantial pressure on the grid.In response,this paper proposes strategic interventions including optimized charging infrastructure planning,time-of-use electricity pricing mechanisms,and smart charging technologies to balance grid loads.The results provide a theoretical foundation for electric vehicle load forecasting,smart grid dispatching,and vehicle-grid integration,thereby enhancing grid operational efficiency and sustainability.
