AIM： To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin （SS） and the expression of SS receptor genes （SSR1mRNA） in rabbits with gastric ul...AIM： To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin （SS） and the expression of SS receptor genes （SSR1mRNA） in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS： Forty rabbits were randomly divided into control group （A）, gastric ulcer model group （B）, FootYangming Meridian group （C）, Foot-Shaoyang Meridian group （D） and Foot-Taiyang Meridian group （E）. The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor（EGF） and somatostatin was measured by radioimmunoassay （PJA）. SS-R1mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS： The value of EGF in model group was obviously lower（73.6±14.8 vs 91.3±14.9 pg/mL, P〈 0.01） than that in control group. The index of gastric ulcer, content of SS and expression of SSRtmRNA in gastric mucosa were significantly higher than those in control group （24.88 ± 6.29 vs 8.50 ± 2.98 scores, P〈 0.01; 2978.6 ± 587.6 vs 1852.4 ± 361.7 mIU/mL, P〈 0.01; 2.56±0.25 vs 1.04±0.36, P〈0.01） . The value of EGF in Foot-Yangming Meridian group was higher than that in model group（92.2±6.7 vs 73.6±14.8 pg/mL, P〈 0.01）. The index of gastric ulcer, content of SS and expression of SS-R1mRNA in gastric mucosa were significantlylower than those in control group（10.88±3.23 vs 24.88±6.29 scores, P〈0.01; 1800.2±488 vs 2978.6±587.6 mIU/mL, P 〈 0.01; 1.07±0.08 vs 2.56±0.25mIU/mL, P〈0.01）. Compared to the model group, the content of SS and expression of SSRlmRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased （2441.0±488. vs 2978.6± 587.6 mIU/mL, P〈 0.05; 1.73±0.16 vs 2.56±0.25 mIU/mL, P〈0.01）. But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Footoyangming Meridian group （P〈0.05） CONCLUSION： Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury. The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSRtmRNA.展开更多
[ Objective] This study aimed to analyze the genetic potymorphism of 39 cassava varieties （lines） preserved in Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops. [ Method ] ISSR primers with ...[ Objective] This study aimed to analyze the genetic potymorphism of 39 cassava varieties （lines） preserved in Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops. [ Method ] ISSR primers with good polymorphisms and amplification effects in cassava were selected for PCR amplification of 39 cassava germplasms, statistics and analysis of the amplified bands were conducted. In addition, cluster analysis was conducted by using UPGMA method based on the genetic similarity among cassava varieties （lines）. [ Result] Ten pairs of ISSR primers were selected. A total of 70 clear electrophoretic bands were amplified, each pair of primers had amplified 3 -9 electrophoretic bands, with an average of 7, and the length of amplified bands ranged from 150 to 2,000 bp; cluster analysis showed that 39 cassava varieties （lines） were clustered into two categories by the similarity coefficient of 0.67 ; in addition, the genetic distance among cassava was very narrow, with genetic similarity coefficients ranging between 0.80 and 1.00. [ Conclusion] This study laid foundation for breeding of high- cmalitv cassava in China.展开更多
基金Supported by the National Natural Science Foundation of China, No.30171136
文摘AIM： To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin （SS） and the expression of SS receptor genes （SSR1mRNA） in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level. METHODS： Forty rabbits were randomly divided into control group （A）, gastric ulcer model group （B）, FootYangming Meridian group （C）, Foot-Shaoyang Meridian group （D） and Foot-Taiyang Meridian group （E）. The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electroacupuncture at points along the above meridians using meridian stimulating instruments for 7 days respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor（EGF） and somatostatin was measured by radioimmunoassay （PJA）. SS-R1mRNA expression in gastric mucosa was determined by RT-PCR. RESULTS： The value of EGF in model group was obviously lower（73.6±14.8 vs 91.3±14.9 pg/mL, P〈 0.01） than that in control group. The index of gastric ulcer, content of SS and expression of SSRtmRNA in gastric mucosa were significantly higher than those in control group （24.88 ± 6.29 vs 8.50 ± 2.98 scores, P〈 0.01; 2978.6 ± 587.6 vs 1852.4 ± 361.7 mIU/mL, P〈 0.01; 2.56±0.25 vs 1.04±0.36, P〈0.01） . The value of EGF in Foot-Yangming Meridian group was higher than that in model group（92.2±6.7 vs 73.6±14.8 pg/mL, P〈 0.01）. The index of gastric ulcer, content of SS and expression of SS-R1mRNA in gastric mucosa were significantlylower than those in control group（10.88±3.23 vs 24.88±6.29 scores, P〈0.01; 1800.2±488 vs 2978.6±587.6 mIU/mL, P 〈 0.01; 1.07±0.08 vs 2.56±0.25mIU/mL, P〈0.01）. Compared to the model group, the content of SS and expression of SSRlmRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased （2441.0±488. vs 2978.6± 587.6 mIU/mL, P〈 0.05; 1.73±0.16 vs 2.56±0.25 mIU/mL, P〈0.01）. But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Footoyangming Meridian group （P〈0.05） CONCLUSION： Electro-acupuncture at Foot-Yangming Meridian can protect gastric mocusa against injury. The mechanism may be releted to the regulation of brain-gut peptides and the expression of SSRtmRNA.
基金Supported by Special Fund for Basic Scientific Research Operation Expenses of Nonprofit Research Institutes Directly Under Guangxi Zhuang Autonomous Region (200803)Guangxi Scientific Research and Technological Development Project (Chairman Fund 09203-03)
文摘[ Objective] This study aimed to analyze the genetic potymorphism of 39 cassava varieties （lines） preserved in Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops. [ Method ] ISSR primers with good polymorphisms and amplification effects in cassava were selected for PCR amplification of 39 cassava germplasms, statistics and analysis of the amplified bands were conducted. In addition, cluster analysis was conducted by using UPGMA method based on the genetic similarity among cassava varieties （lines）. [ Result] Ten pairs of ISSR primers were selected. A total of 70 clear electrophoretic bands were amplified, each pair of primers had amplified 3 -9 electrophoretic bands, with an average of 7, and the length of amplified bands ranged from 150 to 2,000 bp; cluster analysis showed that 39 cassava varieties （lines） were clustered into two categories by the similarity coefficient of 0.67 ; in addition, the genetic distance among cassava was very narrow, with genetic similarity coefficients ranging between 0.80 and 1.00. [ Conclusion] This study laid foundation for breeding of high- cmalitv cassava in China.
