[目的]探讨SPRED1在结肠癌中的表达,及其与结肠癌患者预后的相关性,以及对结肠癌细胞增殖、迁移和凋亡的影响。[方法]采用实时荧光定量聚合酶链反应(qRT-PCR)检测SPRED1 m RNA在结肠癌细胞和组织标本中的表达,免疫组织化学技术检测SPRED...[目的]探讨SPRED1在结肠癌中的表达,及其与结肠癌患者预后的相关性,以及对结肠癌细胞增殖、迁移和凋亡的影响。[方法]采用实时荧光定量聚合酶链反应(qRT-PCR)检测SPRED1 m RNA在结肠癌细胞和组织标本中的表达,免疫组织化学技术检测SPRED1蛋白在结肠癌组织中的表达。使用小干扰RNA(siRNA)干扰结肠癌细胞内SPRED1表达后,CCK-8试剂盒检测结肠癌细胞增殖能力变化,Transwell小室检测结肠癌细胞迁移能力变化,Annexin V-FITC/PI凋亡试剂盒检测细胞凋亡水平。[结果] SPRED1 mRNA在结肠癌细胞株和结肠癌组织中表达水平均显著上调(P均<0.001),高表达SPRED1患者的总生存期短于低表达SPRED1患者(HR=2.326,95%CI:1.340~4.062,P<0.05)。干扰SPRED1后,干扰组结肠癌SW480和SW620细胞在36h和48h后增殖能力显著下降(P<0.05),侵袭能力显著下降(SW480:t=42.05,P<0.0001;SW620:t=12.91,P=0.0002),且凋亡水平显著上升(SW480:t=18.46,P<0.05;SW620:t=18.52,P<0.0001)。[结论]SPRED1在结肠癌中表达上调,促进结肠癌的增殖和侵袭。展开更多
Long noncoding RNAs(lncRNAs)play a crucial regulatory role in the development and progression of multiple cancers.However,the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer...Long noncoding RNAs(lncRNAs)play a crucial regulatory role in the development and progression of multiple cancers.However,the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer remains unclear.In the current study,the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared with primary tumors.Gain-and loss-of-function assays demonstrated that LOC646029 inhibits the proliferation,invasiveness,and metastasis of ovarian cancer cells in vivo and in vitro.Moreover,the downregulation of LOC646029 in metastatic ovarian tumors was strongly correlated with poor prognosis.Mechanistically,LOC646029 served as a miR-627-3p sponge to promote the expression of Sprouty-related EVH1 domain-containing protein 1,which is necessary for suppressing tumor metastasis and inhibiting KRAS signaling.Collectively,our results demonstrated that LOC646029 is involved in the progression and metastasis of ovarian cancer,which may be a potential prognostic biomarker.展开更多
基金supported by the National Key R&D Program of China (No.2021YFC2501000)the CAMS Innovation Fund for Medical Sciences (Nos.2021-I2M-1-018 and 2021-12M-1-067).
文摘Long noncoding RNAs(lncRNAs)play a crucial regulatory role in the development and progression of multiple cancers.However,the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer remains unclear.In the current study,the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared with primary tumors.Gain-and loss-of-function assays demonstrated that LOC646029 inhibits the proliferation,invasiveness,and metastasis of ovarian cancer cells in vivo and in vitro.Moreover,the downregulation of LOC646029 in metastatic ovarian tumors was strongly correlated with poor prognosis.Mechanistically,LOC646029 served as a miR-627-3p sponge to promote the expression of Sprouty-related EVH1 domain-containing protein 1,which is necessary for suppressing tumor metastasis and inhibiting KRAS signaling.Collectively,our results demonstrated that LOC646029 is involved in the progression and metastasis of ovarian cancer,which may be a potential prognostic biomarker.
