This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data ...This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data were retrieved from the clinical database at the Reproductive Center of Peking University Third Hospital(Beijing,China)between January 2011 and December 2022.Couples were included in this retrospective cohort study if they had unexplained or mild male factor infertility and were treated with IUI for two consecutive cycles using the same protocol.A total of 8290 couples were included in the analysis.The mean±standard deviation(s.d.)age of women was 32.0±3.5 years.We categorized groups based on the post-wash TPMSC(×10^(6))levels in the first IUI cycle:group 1(0<TPMSC<1,n=1290),group 2(1≤TPMSC<2,n=863),group 3(2≤TPMSC<3,n=800),group 4(3≤TPMSC<4,n=783),group 5(4≤TPMSC<5,n=1541),group 6(5≤TPMSC<6,n=522),group 7(6≤TPMSC<7,n=547),group 8(7≤TPMSC<8,n=175),group 9(8≤TPMSC<9,n=556),group 10(9≤TPMSC<10,n=192),and group 11(TPMSC≥10,n=1021).The primary outcome was live birth rate of the second IUI cycle.Live birth rates were 7.9%,5.8%,7.6%,7.4%,7.3%,8.4%,7.5%,7.4%,8.8%,8.9%,and 7.6%in each group,respectively.There were no statistically significant differences in clinical pregnancy rates or live birth rates between any groups and those with the post-wash TPMSC<1×10^(6).In an IUI program for unexplained and mild male factor infertility,the post-wash TPMSC in the first IUI cycle was not significantly associated with the live birth rate in the second IUI cycle.展开更多
This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 a...This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.展开更多
[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study w...[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.展开更多
基金supported by National Key Research and Development Program(2022YFC2705305)clinical key project of Peking University Third Hospital(BYSYZD2023007)National Science Fund for Distinguished Young Scholars(81925013).
文摘This study aimed to investigate the associations between the post-wash total progressively motile sperm count(TPMSC)in the first intrauterine insemination(IUI)cycle and pregnancy outcomes of the second IUI cycle.Data were retrieved from the clinical database at the Reproductive Center of Peking University Third Hospital(Beijing,China)between January 2011 and December 2022.Couples were included in this retrospective cohort study if they had unexplained or mild male factor infertility and were treated with IUI for two consecutive cycles using the same protocol.A total of 8290 couples were included in the analysis.The mean±standard deviation(s.d.)age of women was 32.0±3.5 years.We categorized groups based on the post-wash TPMSC(×10^(6))levels in the first IUI cycle:group 1(0<TPMSC<1,n=1290),group 2(1≤TPMSC<2,n=863),group 3(2≤TPMSC<3,n=800),group 4(3≤TPMSC<4,n=783),group 5(4≤TPMSC<5,n=1541),group 6(5≤TPMSC<6,n=522),group 7(6≤TPMSC<7,n=547),group 8(7≤TPMSC<8,n=175),group 9(8≤TPMSC<9,n=556),group 10(9≤TPMSC<10,n=192),and group 11(TPMSC≥10,n=1021).The primary outcome was live birth rate of the second IUI cycle.Live birth rates were 7.9%,5.8%,7.6%,7.4%,7.3%,8.4%,7.5%,7.4%,8.8%,8.9%,and 7.6%in each group,respectively.There were no statistically significant differences in clinical pregnancy rates or live birth rates between any groups and those with the post-wash TPMSC<1×10^(6).In an IUI program for unexplained and mild male factor infertility,the post-wash TPMSC in the first IUI cycle was not significantly associated with the live birth rate in the second IUI cycle.
文摘This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.
基金Supported by Guizhou Provincial Department of Agriculture and Rural Affairs Project(QNYZZZ[2017]No.12,GZSZCYJSTX-04)2024 Quality Supervision and Sampling Project of Normal Temperature Semen for Breeding Pigs.
文摘[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.
