The application of X-ray spectro-microscopy to image changes in the chemical state in application areas such as catalysis,environmental science,or biological samples can be limited by factors such as the speed of meas...The application of X-ray spectro-microscopy to image changes in the chemical state in application areas such as catalysis,environmental science,or biological samples can be limited by factors such as the speed of measurement,the presence of dilute concentrations,radiation damage,and thermal drift during the measurement.We have adapted a reduced-order model approach,known as the discrete empirical interpolation method,which identifies how to optimally subsample the spectroscopic information,accounting for background variations in the signal,to provide an accurate approximation of an equivalent full spectroscopic measurement from the sampled material.This approach uses readily available prior information to guide and significantly reduce the sampling requirements impacting both the total X-ray dose and the acquisition time.The reduced-order model approach can be adapted more broadly to any spectral or spectro-microscopy measurement where a low-rank approximation can be made from prior information on the possible states of a system,and examples of the approach are presented.展开更多
Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems.To this end,nonlinear Raman scattering microscopy,midinfrared photothermal(MIP)microscopy,and a...Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems.To this end,nonlinear Raman scattering microscopy,midinfrared photothermal(MIP)microscopy,and atomic force microscopy(AFM)-based force-detected photothermal microscopies are emerging with better chemical sensitivity,molecular speciffcity,and spatial resolution than conventional vibrational methods.Their utilization in bioimaging applications has provided biological knowledge in unprecedented detail.This Perspective outlines key methodological developments,bioimaging applications,and recent technical innovations of the three techniques.Representative biological demonstrations are also highlighted to exemplify the unique advantages of obtaining vibrational contrasts.With years of effort,these three methods compose an expanding vibrational bioimaging toolbox to tackle speciffc bioimaging needs,beneffting many biological investigations with rich information in both label-free and labeling manners.Each technique will be discussed and compared in the outlook,leading to possible future directions to accommodate growing needs in vibrational bioimaging.展开更多
文摘The application of X-ray spectro-microscopy to image changes in the chemical state in application areas such as catalysis,environmental science,or biological samples can be limited by factors such as the speed of measurement,the presence of dilute concentrations,radiation damage,and thermal drift during the measurement.We have adapted a reduced-order model approach,known as the discrete empirical interpolation method,which identifies how to optimally subsample the spectroscopic information,accounting for background variations in the signal,to provide an accurate approximation of an equivalent full spectroscopic measurement from the sampled material.This approach uses readily available prior information to guide and significantly reduce the sampling requirements impacting both the total X-ray dose and the acquisition time.The reduced-order model approach can be adapted more broadly to any spectral or spectro-microscopy measurement where a low-rank approximation can be made from prior information on the possible states of a system,and examples of the approach are presented.
基金the support from NIH Director’s New Innovator Award,DP2 GM140919-01.
文摘Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems.To this end,nonlinear Raman scattering microscopy,midinfrared photothermal(MIP)microscopy,and atomic force microscopy(AFM)-based force-detected photothermal microscopies are emerging with better chemical sensitivity,molecular speciffcity,and spatial resolution than conventional vibrational methods.Their utilization in bioimaging applications has provided biological knowledge in unprecedented detail.This Perspective outlines key methodological developments,bioimaging applications,and recent technical innovations of the three techniques.Representative biological demonstrations are also highlighted to exemplify the unique advantages of obtaining vibrational contrasts.With years of effort,these three methods compose an expanding vibrational bioimaging toolbox to tackle speciffc bioimaging needs,beneffting many biological investigations with rich information in both label-free and labeling manners.Each technique will be discussed and compared in the outlook,leading to possible future directions to accommodate growing needs in vibrational bioimaging.
