This study explores the broad-spectrum application of OsRALF26,a small secreted peptide belonging to the rapid alkalinization factor(RALF)family in rice.We found that the rice genome carries numerous lineage-specific ...This study explores the broad-spectrum application of OsRALF26,a small secreted peptide belonging to the rapid alkalinization factor(RALF)family in rice.We found that the rice genome carries numerous lineage-specific OsRALFs,suggesting that this evolutionary expansion could be the result of an arms race with pathogens.Among them,we focused on the Oryza-specific Os RALF26 and its closest homolog,OsRALF27,analyzing their effects across a range of plant species from monocots to dicots.The exogenous application of OsRALF26 significantly reduced bacterial populations in rice challenged with Xanthomonas oryzae pv.oryzae(Xoo)and in Arabidopsis and tomato challenged with Pseudomonas syringae pv.tomato DC3000(Pst DC3000),whereas Os RALF27 did not enhance resistance.展开更多
The desensitization effect of the hypoallergenic infant formula products is critical for production quality,and establishment of sensitive and accurate determination method of allergens,such as α_(s1)-casein and α_(...The desensitization effect of the hypoallergenic infant formula products is critical for production quality,and establishment of sensitive and accurate determination method of allergens,such as α_(s1)-casein and α_(s2)-casein,remains challengeable in the field of infant formula industry.Therefore,the objective of this study was to identify specific peptides(YLGYLEQLLR and FALPQYLK)from α_(s1)-casein and α_(s2)-casein in infant formula by orbitrap high resolution mass spectrometry(Orbitrap MS),then establish an analytical method for the qualitative and quantitative determination of α_(s1)-casein and α_(s2)-casein based on extended specific peptide-isotope-labeled internal standard dilution-LC-MS/MS.Specific peptides obtained by trypsin enzymolysis of hypoallergenic infant formula were separated by Waters Acquity LC HSS T3 column,and analyzed under ESI positive ion mode using multireaction monitoring(MRM).The specific peptides of α_(s1)-casein and α_(s2)-casein exhibited linear relationships within the concentration range of 0.0025-1.5μmol/L and correlation coefficients were above 0.99.The detection limits of α_(s1)-casein and α_(s2)-casein were 0.3 mg/kg,with quantification limits of 1.0 mg/kg,respectively.The average recoveries were 83.5%-87.3%(α_(s1)-casein)and 80.1%-84.4%(α_(s2)-casein),with RSDs of 2.3%-3.7%(α_(s1)-casein)and 2.5%-5.0%(α_(s2)-casein),respectively.Our results demonstrated that this method is capable of analyzing the content of α_(s1)-casein and α_(s2)-casein in hypoallergenic infant formula with excellent sensitivity and specificity.展开更多
Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electroche...Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.展开更多
基金supported by the National Research Foundation of Korea grant funded by the Korea government(Grant Nos.NRF-2020R1A2C1007778 and RS-2024-00454908)。
文摘This study explores the broad-spectrum application of OsRALF26,a small secreted peptide belonging to the rapid alkalinization factor(RALF)family in rice.We found that the rice genome carries numerous lineage-specific OsRALFs,suggesting that this evolutionary expansion could be the result of an arms race with pathogens.Among them,we focused on the Oryza-specific Os RALF26 and its closest homolog,OsRALF27,analyzing their effects across a range of plant species from monocots to dicots.The exogenous application of OsRALF26 significantly reduced bacterial populations in rice challenged with Xanthomonas oryzae pv.oryzae(Xoo)and in Arabidopsis and tomato challenged with Pseudomonas syringae pv.tomato DC3000(Pst DC3000),whereas Os RALF27 did not enhance resistance.
基金supported by Zhejiang Provincial Natural Science Foundation of China under Grant No.LTGY23H260007Science Project of Zhejiang Provincial Administration for Market Regulation No.20210126+2 种基金Science Project of Zhejiang Medical Product Administration No.2023025Science Technology Department of Zhejiang Province No.2024C01248(SD2)Quzhou City Science and Quzhou City Science and Technology Plan Project No.2023K100.
文摘The desensitization effect of the hypoallergenic infant formula products is critical for production quality,and establishment of sensitive and accurate determination method of allergens,such as α_(s1)-casein and α_(s2)-casein,remains challengeable in the field of infant formula industry.Therefore,the objective of this study was to identify specific peptides(YLGYLEQLLR and FALPQYLK)from α_(s1)-casein and α_(s2)-casein in infant formula by orbitrap high resolution mass spectrometry(Orbitrap MS),then establish an analytical method for the qualitative and quantitative determination of α_(s1)-casein and α_(s2)-casein based on extended specific peptide-isotope-labeled internal standard dilution-LC-MS/MS.Specific peptides obtained by trypsin enzymolysis of hypoallergenic infant formula were separated by Waters Acquity LC HSS T3 column,and analyzed under ESI positive ion mode using multireaction monitoring(MRM).The specific peptides of α_(s1)-casein and α_(s2)-casein exhibited linear relationships within the concentration range of 0.0025-1.5μmol/L and correlation coefficients were above 0.99.The detection limits of α_(s1)-casein and α_(s2)-casein were 0.3 mg/kg,with quantification limits of 1.0 mg/kg,respectively.The average recoveries were 83.5%-87.3%(α_(s1)-casein)and 80.1%-84.4%(α_(s2)-casein),with RSDs of 2.3%-3.7%(α_(s1)-casein)and 2.5%-5.0%(α_(s2)-casein),respectively.Our results demonstrated that this method is capable of analyzing the content of α_(s1)-casein and α_(s2)-casein in hypoallergenic infant formula with excellent sensitivity and specificity.
基金financially supported by National Key Research and Development Program of China(2022YFC2601604)Major science and technology project of Yunnan Province(202202AE090085)+9 种基金the National Natural Science Foundation of China(3216059732160236)Science and technology talent and platform plan of YunnanKey Scientific and Technology Project of Yunnan(202203AC100010)Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)the second phase of“Double-First Class”program construction of Yunnan Universitygrants from State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University(2021KF005)Key Scientific and Technology Project of Yunnan(202002AE320005)Program for Excellent Young Talents of Yunnan Universitythe Program for Donglu Scholars of Yunnan University。
文摘Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.
