Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GH...Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GHNBS)and its 5L flanking sequence have been cloned by race and tail PCR and further studied.The entire coding region is 2583 bp and enco des a polypeptide of 861 a mino acids with 28%maximum homology to an R gene of Arabi dopsis depo sited in the GenBank.Semi quantitativeRT-PCR showed thatGHNBS was expressed in floral buds,petals,phloem,root s,and leaves,and it has a greater exp ression pattern in roots and leaves.展开更多
The leaf streak of rice is one of the seedborneand quarantine bacterial disease in China.Therice seeds must be strictly inspected to preventthe disease from spreading.The serologicaltest is a rapid and accurate method...The leaf streak of rice is one of the seedborneand quarantine bacterial disease in China.Therice seeds must be strictly inspected to preventthe disease from spreading.The serologicaltest is a rapid and accurate method of identify-ing seedborne bacterial plant pathogens.Butthe specificity and thus reliability of any sero-logical method depend first on the specificity ofthe antiserum.Therefore,a high specificity of展开更多
The Aquatic Quarantine Facility （AQF） for Litopenaeus vannamei established under the Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture is located on the eastern coastal region of Chenna...The Aquatic Quarantine Facility （AQF） for Litopenaeus vannamei established under the Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture is located on the eastern coastal region of Chennai in Tamil Nadu, India. The facility is an approved quarantine premise for the SPF vannamei broodstock imported across India and is probably the only one of its kind to be established in Southeast Asia. The centre is managed and operated by Rajiv Gandhi Centre for Aquaculture （RGCA）, a technical extension of the Marine Products Export Development Authority （MPEDA）, Ministry of Commerce ＆ Industry, Government of India. The quarantine facility functions on the basis of strict biosecurity protocol and as per the guidelines stipulated in Standard Operating Procedures （SOP） framed by a team of experts. The facility ensures specific pathogen free status of the SPF L. vannamei broodstock imported to India, thus playing key role in restricting the entry of diseases to the Country. This article presents the SPF certification process of the imported L. vannamei carried out at the facility.展开更多
文摘Cotton disea ses represent a major challenge to cotton growth.Cloning of a cotton pathogen re-sponse gene and promoter is of great importance to improve disease resistance.In this study,a full length CC-NBS-LRRgene(GHNBS)and its 5L flanking sequence have been cloned by race and tail PCR and further studied.The entire coding region is 2583 bp and enco des a polypeptide of 861 a mino acids with 28%maximum homology to an R gene of Arabi dopsis depo sited in the GenBank.Semi quantitativeRT-PCR showed thatGHNBS was expressed in floral buds,petals,phloem,root s,and leaves,and it has a greater exp ression pattern in roots and leaves.
文摘The leaf streak of rice is one of the seedborneand quarantine bacterial disease in China.Therice seeds must be strictly inspected to preventthe disease from spreading.The serologicaltest is a rapid and accurate method of identify-ing seedborne bacterial plant pathogens.Butthe specificity and thus reliability of any sero-logical method depend first on the specificity ofthe antiserum.Therefore,a high specificity of
文摘The Aquatic Quarantine Facility （AQF） for Litopenaeus vannamei established under the Department of Animal Husbandry, Dairying and Fisheries, Ministry of Agriculture is located on the eastern coastal region of Chennai in Tamil Nadu, India. The facility is an approved quarantine premise for the SPF vannamei broodstock imported across India and is probably the only one of its kind to be established in Southeast Asia. The centre is managed and operated by Rajiv Gandhi Centre for Aquaculture （RGCA）, a technical extension of the Marine Products Export Development Authority （MPEDA）, Ministry of Commerce ＆ Industry, Government of India. The quarantine facility functions on the basis of strict biosecurity protocol and as per the guidelines stipulated in Standard Operating Procedures （SOP） framed by a team of experts. The facility ensures specific pathogen free status of the SPF L. vannamei broodstock imported to India, thus playing key role in restricting the entry of diseases to the Country. This article presents the SPF certification process of the imported L. vannamei carried out at the facility.
