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A New Exploration of Artificially Induced Spalted Wood of Two Fungi:Hypoxylon and Sistotrema
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作者 Yan Yan Junpeng Dong +3 位作者 Haibo Hu Susu Yang Lin Liu Lei Qin 《Journal of Renewable Materials》 EI 2023年第11期3907-3916,共10页
One strain of Hypoxylon sp.CXM-3 and one strain of Sistotrema brinkmannii CXM-4 were inoculated onto sterilized cherry,poplar,birch,and basswood sheets in a certain shape and incubated at constant temperature and humi... One strain of Hypoxylon sp.CXM-3 and one strain of Sistotrema brinkmannii CXM-4 were inoculated onto sterilized cherry,poplar,birch,and basswood sheets in a certain shape and incubated at constant temperature and humidity for 4,8,12,and 16 weeks,respectively,to analyze whether the grain pattern formed by the zone lines was consistent with the predetermined pattern.The results showed that the zone lines of CXM-3 of Hypoxylon were free,delicate,and soft,with brown lines and black staining,mostly accompanied by black and brown dots,facets,and clusters,while the zone lines of CXM-4 of Sistotrema brinkmannii grew along the predetermined grain,with strong lines and a clean surface.After inoculation and incubation at a constant temperature of 25℃±2℃ and humidity of 60%,Sistotrema brinkmannii CXM-4,on basswood veneer at 4-8 weeks and cherry veneer at 4-16 weeks,were able to develop zone lines following a predetermined grain.Artificially induced spalted wood can only maintain a large shape,which cannot guarantee that the pattern of large-scale production of spalted wood is exactly the same and cannot be accurate to the minute details.The artificial induction can thus result in the formation of a predetermined grain pattern of the mottled wood,thus enhancing the product value and artistic value of solid wood furniture and crafts. 展开更多
关键词 spalted wood artificially induced GRAIN
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spalt major位于Wg/Wnt和Dpp信号通路下游调控豌豆蚜翅型分化
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作者 陈锦华 肖荷 +2 位作者 宗鑫 沈杰 王丹 《昆虫学报》 北大核心 2025年第5期569-575,共7页
【目的】本研究旨在通过探究翅发育调控基因spalt major在豌豆蚜Acyrthosiphon pisum翅型分化中的作用,进一步揭示昆虫翅非遗传多型现象的分子机制。【方法】在实验室条件下用密度诱导孤雌生殖的无翅豌豆蚜产生高比例的有翅型后代并利用... 【目的】本研究旨在通过探究翅发育调控基因spalt major在豌豆蚜Acyrthosiphon pisum翅型分化中的作用,进一步揭示昆虫翅非遗传多型现象的分子机制。【方法】在实验室条件下用密度诱导孤雌生殖的无翅豌豆蚜产生高比例的有翅型后代并利用qRT-PCR检测Apsal的表达量。设计合成靶向豌豆蚜spalt major基因Apsal的dsRNA(dsApsal),以及Apsal上游Wg/Wnt和Dpp信号通路基因Wnt-2(ApWnt2)和decapentaplegic(Apdpp)的dsRNA(分别为dsApWnt2和dsApdpp)。运用纳米载体介导的体壁渗透法干扰无翅母蚜和其初孵若蚜的Apsal,ApWnt2和Apdpp,记录有翅率,并利用qRT-PCR检测基因表达量。【结果】高密度处理豌豆蚜母蚜可以稳定诱导出超过80%的有翅后代;与单独饲养的无翅蚜相比,翅发育关键基因Apsal在密度诱导的有翅子代中表达量升高;与对照组(dseGFP)相比,dsApsal干扰Apsal表达造成有翅率显著降低;干扰上游基因ApWnt2和Apdpp的表达,Apsal的表达量降低。【结论】Apsal参与调控豌豆蚜翅型分化,其表达同时受Wg/Wnt和Dpp信号通路激活。本研究进一步揭示了蚜虫翅型分化的分子机制,为阻止蚜虫迁飞转移寄主提供了RNA防治的理论和技术依据。 展开更多
关键词 豌豆蚜 翅型分化 Wg/Wnt信号通路 SPALT RNAi
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Stain capacity of three fungi on two fast-growing wood 被引量:3
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作者 Taize Song Fangchao Cheng Jianping Sun 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第1期427-434,共8页
We investigated the stain of fast-growing wood(Cunninghamia lanceolate,CL;Paulownia,PT)inoculated with three fungi(Arthrinium phaeospermum,AP;Vibrio anguillarum,VA;Aspergillacea,AS)to explore the new wood dyeing ways ... We investigated the stain of fast-growing wood(Cunninghamia lanceolate,CL;Paulownia,PT)inoculated with three fungi(Arthrinium phaeospermum,AP;Vibrio anguillarum,VA;Aspergillacea,AS)to explore the new wood dyeing ways and the better combination of wood and fungi for dyeing.Only AP could dye on CL and PT.Especially for CL,its percentage of internal spalting,percentage of external spalting and dyeing depth were the highest(48%,15%and 5.06 mm,respectively).Surprisingly,the bigger weight loss occurs on PT.The results showed that the dyeing eff ect of AP dyeing CL was the best,and the wood color change was obviously(Orange to dark red).AP could produce more pigments than the other two fungi(VA;AS),CL was more suitable for fungus staining than PT,indicating that AP could off ered a new potential market and a chance for areas to earning higher income for CL.This research paves the way for improving color change was obviously(Orange to dark red).AP could produce more pigments than the other two fungi(VA;AS),CL was more suitable for fungus staining than PT,indicating that AP could off er a new potential market and a chance for areas to earn higher income for CL. 展开更多
关键词 spalted wood Cunninghamia lanceolata PAULOWNIA Arthrinium phaeospermum Vibrio anguillarum Aspergillacea
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宫颈癌组织中SALL3、DNMT3A、甲基化SALL3基因表达观察 被引量:1
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作者 赵娟 魏星 +2 位作者 杨婷 赵敏伊 杨筱凤 《山东医药》 CAS 2019年第36期1-4,共4页
目的观察宫颈癌组织中spalt样转录因子3(SALL3)、DNA甲基转移酶3A(DNMT3A)、甲基化SALL3基因表达变化,并探讨其意义。方法宫颈癌组织200例份、正常宫颈组织40例份,采用qRT-PCR法检测其中的SALL3 mRNA、DNMT3A mRNA,分析宫颈癌组织中SALL... 目的观察宫颈癌组织中spalt样转录因子3(SALL3)、DNA甲基转移酶3A(DNMT3A)、甲基化SALL3基因表达变化,并探讨其意义。方法宫颈癌组织200例份、正常宫颈组织40例份,采用qRT-PCR法检测其中的SALL3 mRNA、DNMT3A mRNA,分析宫颈癌组织中SALL3、DNMT3A mRNA表达的相关性及其与宫颈癌患者临床病理参数的关系;采用甲基化特异PCR法检测其中的甲基化SALL3基因并计算甲基化率、相对甲基化程度。结果宫颈癌组织中SALL3 mRNA、DNMT3A mRNA相对表达量分别为0.253±0.051、0.272±0.055,正常宫颈组织中SALL3 mRNA、DNMT3A mRNA相对表达量分别为1.050±0.062、0.093±0.016,两者相比,P均<0.05。宫颈癌组织中SALL3 mRNA、DNMT3A mRNA表达呈负相关(r=-0.679,P=0.000)。SALL3 mRNA、DNMT3A mRNA表达与宫颈癌FIGO分期、分化程度有关(P均<0.05)。宫颈癌组织中SALL3基因甲基化率为60.5%(121/200),相对甲基化程度为1.72±0.61;正常宫颈组织中SALL3基因甲基化率为30.0%(12/40),相对甲基化程度为0.26±0.07;两者相比,P均<0.05。结论宫颈癌组织中SALL3 mRNA低表达、DNMT3A mRNA高表达,两者负相关,且与宫颈癌FIGO分期、分化程度有关;宫颈癌组织中SALL3基因甲基化率及相对甲基化程度升高。 展开更多
关键词 spalt样转录因子3 DNA甲基转移酶3A DNA甲基化 宫颈肿瘤 宫颈癌
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坐骨神经分支选择损伤小鼠腰段脊髓背角spalt样转录因子1(Sall1)表达下调且炎症因子表达增强 被引量:4
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作者 陆培春 张进 +4 位作者 徐静 蒋锦 孙彩霞 吴进 苏兆亮 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2020年第2期111-116,共6页
目的探讨spalt样转录因子1(Sall1)在坐骨神经分支选择损伤的神经病理性疼痛模型小鼠腰段脊髓背角中的表达及定位。方法6~8周龄BALB/c小鼠随机分成对照组、假手术组和坐骨神经分支选择损伤组(SNI组)。在模型建立之前的1 d及术后第3、7、1... 目的探讨spalt样转录因子1(Sall1)在坐骨神经分支选择损伤的神经病理性疼痛模型小鼠腰段脊髓背角中的表达及定位。方法6~8周龄BALB/c小鼠随机分成对照组、假手术组和坐骨神经分支选择损伤组(SNI组)。在模型建立之前的1 d及术后第3、7、10、14天,测定小鼠机械缩足反应阈值(MWT);分别于手术建模后3、7、10、14 d,收集小鼠L4~6节段的患侧脊髓背角部分,反转录PCR检测Sall1 mRNA表达及术后7 d炎症因子白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)的mRNA水平;Western blot法测定上述4个时间点的Sall1蛋白含量,免疫组织化学染色法检测术后3、7、10、14 d脊髓Sall1的表达定位并进行半定量分析。结果与对照组、假手术组相比,SNI组术后3、7、10、14 d,MWT均下降;与对照组相比,SNI组Sall1 mRNA和蛋白水平在术后7、10、14 d均下降,术后7 d,IL-1β、IL-6、TNF-αmRNA水平增加;对照组、SNI组Sall1主要定位于脊髓背角,且术后除3 d外各时间点SNI组的含量明显低于对照组。结论Sall1定位于脊髓背角,坐骨神经分支选择损伤可致患侧腰段脊髓背角的Sall1表达下降,IL-1β、IL-6、TNF-αmRNA表达增加。 展开更多
关键词 spalt样转录因子1(Sall1) 神经病理性疼痛 白细胞介素1β(IL-1β) IL-6 肿瘤坏死因子α(TNF-α)
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