Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the q...Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.展开更多
A zinc sulfate open framework matrix,[Zn(SO_4)(DMSO)](1),was synthesized by solvothermal evaporationusing dimethyl sulfoxide(DMSO)as the solvent.A compositeP@1,which exhibits fluorescence and room tempera-ture phospho...A zinc sulfate open framework matrix,[Zn(SO_4)(DMSO)](1),was synthesized by solvothermal evaporationusing dimethyl sulfoxide(DMSO)as the solvent.A compositeP@1,which exhibits fluorescence and room tempera-ture phosphorescence(RTP)properties,was prepared by doping 2,6-naphthalic acid(P)into matrix1at a low con-centration.P@1emitted a green RTP that was visible to the naked eye and lasted for approximately 2 s.P@1exhib-ited selective phosphorescence enhancement response towards Pb^(2+),with a detection limit of 2.52μmol·L^(-1).Themain detection mechanism is the Pb—O coordination-induced phosphorescence enhancement in the system.Inter-estingly,P@1also functioned as a dual-channel probe for the rapid detection of Fe^(3+)ions through fluorescencequenching with a detection limit of 0.038μmol·L^(-1).The recognition mechanism may be attributed to the competi-tive energy absorption betweenP@1and Fe^(3+)ions.CCDC:2388502,1.展开更多
Polarity,as a crucial environmental characteristic,plays a significant role in numerous cellular physiological processes.Abnormal changes in polarity are closely associated with various diseases.However,existing tools...Polarity,as a crucial environmental characteristic,plays a significant role in numerous cellular physiological processes.Abnormal changes in polarity are closely associated with various diseases.However,existing tools still have certain limitations that hinder accurate detection of polarity.Therefore,there is a pressing need to develop powerful tools for precisely monitoring changes in polarity.In this study,we developed two dual-emissive fluorogenic dyes by innovatively introducing 1,3-dithio-2-heteroarsenic cyclopentane and 1,2-diselenocyclopentane respectively into the near-infrared(NIR)coumarin-benzopyranium skeleton to enhance their cellular uptake capability.Additionally,we synthesized the polarity-sensitive dual-emissive fluorogenic probe CSFNS,which exhibits high cellular uptake rate,by modifying the spironolactone(Aldactone)structure of CBA into spirolactam.CSFNS not only demonstrates excellent polarity sensitivity in vitro but is also successfully applied to visually monitor the polarity changes in various types of living cells,including healthy cells,cancer cells and drug-induced senescent cells.展开更多
以三苯胺衍生物为原料,通过引入不同苯胺衍生物,构建了4种席夫碱荧光探针(FY1—FY4)用于Zn^(2+)的检测,其结构均用1 H NMR进行了表征.实验结果表明,探针FY1—FY4对Zn^(2+)都显示出特异性响应及良好的抗干扰能力.当Zn^(2+)浓度在一定范...以三苯胺衍生物为原料,通过引入不同苯胺衍生物,构建了4种席夫碱荧光探针(FY1—FY4)用于Zn^(2+)的检测,其结构均用1 H NMR进行了表征.实验结果表明,探针FY1—FY4对Zn^(2+)都显示出特异性响应及良好的抗干扰能力.当Zn^(2+)浓度在一定范围内时,探针FY1—FY4的荧光强度与Zn^(2+)浓度均呈良好的线性关系.其中,探针FY2的检测阈值最低,为1.15μmol/L.Job's Plot实验表明,探针FY1—FY4与Zn^(2+)配位的化学计量比约为2∶1.展开更多
A novel fluorescent Zn^2＋ probe based on 6-methoxyquinolin was synthesized in four steps from inexpensive starting materials. It exhibits very strong fluorescence responses and has a remarkably high selectivity to Z...A novel fluorescent Zn^2＋ probe based on 6-methoxyquinolin was synthesized in four steps from inexpensive starting materials. It exhibits very strong fluorescence responses and has a remarkably high selectivity to Zn^2＋ than other physiological relevant metal ions. This new compound could be used as low-priced yet high-quality Zn^2＋ probe.展开更多
In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with ...In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with excellent selectivity. The reaction mechanism was explained by means of absorption, fluorescence and HPLC-MS.展开更多
Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synth...Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synthesized a novel near-infrared intramolecular charge transfer(ICT)based ratiometric fluorescent probe to detect Fe^(2+),by using naphthalimide and indole moieties as building blocks.Our work showed that the radiometric probe has excellent selectivity,sensitivity and rapid response.Moreover,we could successfully perform real-time monitoring of Fe^(2+) in He La cells and C.elegans.展开更多
The mercury species in the ocean(MeHg,Hg^(2+))will be enriched in marine organisms and threaten human health through the food chain.While the excessive H_(2)O_(2)in the metabolic process will produce hydroxyl radicals...The mercury species in the ocean(MeHg,Hg^(2+))will be enriched in marine organisms and threaten human health through the food chain.While the excessive H_(2)O_(2)in the metabolic process will produce hydroxyl radicals and accelerate the aging of human cells,causing a series of diseases.Hence,the cost-effective and rapid detection of mercury and H_(2)O_(2)is of urgent requirement and significance.Here,we synthesized emerging graphitic carbon nitride quantum dots(g-CNQDs)with high fluorescence quantum yield(FLQY)of 42.69%via a bottom-up strategy by a facile one-step hydrothermal method.The g-CNQDs can detect the H_(2)O_(2)and Hg^(2+)through the fluorescence quenching effect between g-CNQDs and detected substances.With the presence of KI,g-CNQDs show concentration-dependent fluorescence toward H_(2)O_(2),with a wide detection range of 1–1000μmolL^(-1)and a low detection limit of 0.23μmolL^(-1).The g-CNQDs also show sensitivity toward Hg^(2+)with a detection range of 0–0.1μmolL^(-1)and a detection limit of 0.038μmolL^(-1).This dual-function detection of g-CNQDs has better practical application capability compared to other quantum dot detection.This study may provide a new strategy for g-CNQDs preparation and construct a fluorescence probe that can be used in various systems involving H_(2)O_(2)and Hg^(2+),providing better support for future bifunctional or multifunction studies.展开更多
Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmo...Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmol/L)with good selectivity and anti-interference ability.In addition,IDNA has satisfactory photostability in HeLa cells,ability of mitochondrial co-localization,and can be utilized in fluorescence imaging of H_(2)S.展开更多
We have developed a simple method for the preparation of highly fluorescent and stable, water-soluble CdTe quantum dots in sol-gel-derived composite silica spheres which were coated with calix[6]arene. The resulting n...We have developed a simple method for the preparation of highly fluorescent and stable, water-soluble CdTe quantum dots in sol-gel-derived composite silica spheres which were coated with calix[6]arene. The resulting nanoparticles (NPs) were characterized in terms of UV, fluorescence and FT-IR spectroscopy and TEM. The results show that the new NPs display more intense fluorescence intensity and are more stable than its precursors of the type SiO2/CdTe. Under the optimum, the novel NPs exhibit a higher selectivity and ultrasensitive fluorescence probes for the determination of gly-phosate over other pesticides, the fluorescence intensity increase with the concentration of glyphosate in the range from 1.0 to 25.0 nmol/L and the detection limit is low to 0.0725 nmol/L. A mechanism is suggested to explain the inclusion process by a Langmuir binding isotherm.展开更多
Most reported fluorescent probes have limitations in practical applications in living systems due to the strong autofluorescence background,construction of probes with near-infrared（NIR） fluorescence emission is an ...Most reported fluorescent probes have limitations in practical applications in living systems due to the strong autofluorescence background,construction of probes with near-infrared（NIR） fluorescence emission is an accessible approach for addressing this challenge.We here designed a NIR fluorescent probe for monitoring the endogenous production of H2S in living cells.The designed probe showed significant NIR fluorescence turn-on response to H2S with high selectivity,enabling the sensitive detection H2S.Importantly,the probe could be applied in monitoring the endogenous production of H2S in raw 264.7 macrophages.This study showed that fluvastatin can promote the activity of cystathionineγ-lyase（CSE） for generation H2S.展开更多
H2S is an essential gas signal molecule in cells,and viscosity is a key internal environmental parameter.Recent studies have shown that H_(2)S acts as a cytoarchitecture agent and gas transmitter in many tissues,e.g.,...H2S is an essential gas signal molecule in cells,and viscosity is a key internal environmental parameter.Recent studies have shown that H_(2)S acts as a cytoarchitecture agent and gas transmitter in many tissues,e.g.,as a regulator of neuroendocrine in the brain for mediating vascular tone in blood vessels.Mitochondrial viscosity is an important parameter for judging whether mitochondrial function is normal.It has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson’s disease(PD),and the protective role of H_(2)S in PD models has been extensively demonstrated.Herein,Mito-HS,a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H_(2)S content.Moreover,this probe could detect the relative amount of and changes in mitochondrial H2S in situ due to the reduced mitochondrial targeting ability after reaction with H_(2)S.The results show that H2S in mitochondria is inversely related to viscosity.The PD model has a lower H2S in mitochondria and a higher mitochondrial viscosity than did the normal.This result is important for our deep understanding of PD and its causes.展开更多
The fundamental momentum conservation requirement q - 0 for the Raman process is relaxed in the nanocrystal- lites （NCs）, and phonons away from the Brillouin-zone center will be involved in the Raman scattering, whi...The fundamental momentum conservation requirement q - 0 for the Raman process is relaxed in the nanocrystal- lites （NCs）, and phonons away from the Brillouin-zone center will be involved in the Raman scattering, which is well-known as the phonon confinement effect in NCs. This usually gives a downshift and asymmetric broadening of the Raman peak in various NCs. Recently, the A1 mode of 1L MoS2 NCs is found to exhibit a blue shift and asymmetric broadening toward the high-frequency side [Chem. Soc. Rev. 44 （2015） 2757 and Phys. Rev. B 91 （2015） 195411]. In this work, we carefully check this issue by studying Raman spectra of lL MoS2 NCs prepared by the ion implantation technique in a wide range of ion-implanted dosage. The same confinement coefficient is used for both E＇ and A＇1 modes in 1L MoS2 NCs since the phonon uncertainty in an NC is mainly determined by its domain size. The asymmetrical broadening near the A＇1 and E＇ modes is attributed to the appearance of defect-activated phonons at the zone edge and the intrinsic asymmetrical broadening of the two modes, where the anisotropy of phonon dispersion curves along Г-K and Г- M is also considered. The photoluminescence spectra confirm the formation of small domain size of 1L MoS2 nanocrystallites in the ion-implanted 1L MoS2. This study provides not only an approach to quickly probe phonon dispersion trends of 2D materials away from Г by the Raman scattering of the corresponding NCs, but also a reference to completely understand the confinement effect of different modes in various nanomaterials.展开更多
Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical prop...Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.展开更多
By means of total energy calculations within the framework of the local density approximation (LDA), the interactions between a silicon Si(001) surface and a scanning probe are investigated. The tip of the probe, comp...By means of total energy calculations within the framework of the local density approximation (LDA), the interactions between a silicon Si(001) surface and a scanning probe are investigated. The tip of the probe, comprising 4 Si atoms scans along the dimer lines above an asymmetric p(2 × 1) surface, at a distance where the chemical interaction between tip-surface is dominant and responsible for image resolution. At that distance, the tip causes the dimer to toggle when it scans above the lower atom of a dimer. The toggled dimers create an alternating pattern, where the immediately adjacent neighbours of a toggled dimer remain unchanged. After the tip has fully scanned across the p(2 × 1) surface, causes the dimers to arrange in a p(2 × 2) reconstruction, reproducing the images obtained in scanning probe experiments. Our modelling methodology includes simulations that reveal the energy input required to overcome the barrier to the onset of dimer toggling. The results show that the energy input to overcome this barrier is lower for the p(2 × 1) surface than that for the p(2 × 2) or c(4 × 2) surfaces.展开更多
Tumor-related PD-L2 expression is associated with the clinical efficacy of PD-1/PD-L1 blockade therapy.PD-L2-specific imaging can help selecting patients for appropriate immunotherapy.In this study,a PD-L2-targeting p...Tumor-related PD-L2 expression is associated with the clinical efficacy of PD-1/PD-L1 blockade therapy.PD-L2-specific imaging can help selecting patients for appropriate immunotherapy.In this study,a PD-L2-targeting peptide(PDP2)was screened by the one-bead one-compound combinatorial library approach.Using the retro-inverso D-peptide of PDP2(RD-PDP2)and PEGylation strategies,we developed a novel Tc-99m-labeled PD-L2-targeting peptide as a SPECT tracer(^(99m)Tc-PEG_(6)-RD-PDP2)for imaging of tumor PD-L2 expression.The radiolabeling yield of ^(99m)Tc-PEG_(6)-RD-PDP2 was greater than 95%by the standard HYNIC/tricine/TPPTS labeling procedure.^(99m)Tc-PEG_(6)-RD-PDP2 displayed high PD-L2-binding specificity both in vitro and in vivo.SPECT/CT imaging with^(99m)Tc-PEG_(6)-RD-PDP2 showed that the A549-PD-L2tumors were clearly visualized,whereas the signals in PD-L2-negative A549 tumors were much lower.In vivo blocking study suggested that the tumor uptake of^(99m)Tc-PEG_(6)-RD-PDP2 was PD-L2 specifically mediated.^(99m)Tc-PEG_(6)-RD-PDP2 is a promising SPECT probe for the non-invasive imaging of tumor PD-L2expression and has a great potential in guiding the anti-PD-1 or anti-PD-L1 immunotherapy of cancer.展开更多
As a strong oxidizer,hypochlorite(Cl O^(-))are widely employed as bleaching agents and disinfectants.Determination of ClO^(-)is required to ensure bactericidal effects and avoid hazards caused by excessive residual ch...As a strong oxidizer,hypochlorite(Cl O^(-))are widely employed as bleaching agents and disinfectants.Determination of ClO^(-)is required to ensure bactericidal effects and avoid hazards caused by excessive residual chlorine.Herein,the derivative bicyclic 2-pyridone,namely DHIP-Py,was prepared successfully to establish a new ClO^(-)-quantitative method.The probe exhibits excellent Cl O^(-)selectivity over other ROS and anions/cations,high sensitivity(LOD=1.32μmol/L),fast response(<5 s),and wide-p H tolerance(pH4~10).Benefit from its good water solubility,DHIP-Py is well suited for water sample analysis and has been successfully applied to detect Cl O^(-)in real-world food and environmental samples,including tap water,bottled water and river water.The detection results were essentially identical to that of obtained from traditional DPD method.Moreover,visual detection of Cl O^(-)via filter paper-based solid sensor and imaging of ClO^(-)in Escherichia coli were also achieved by DHIP-Py.These satisfactory results demonstrate that this bicyclic 2-pyridone-based hypochlorite probe is a promising free chlorine chemosensor with great potential for analytical applications.展开更多
A newly designed divertor Langmuir probe diagnostic system has been installed in a rare closed divertor of the HL-2A tokamak and steadily operated for the study of divertor physics involved edge-localized mode mitigat...A newly designed divertor Langmuir probe diagnostic system has been installed in a rare closed divertor of the HL-2A tokamak and steadily operated for the study of divertor physics involved edge-localized mode mitigation,detachment and redistribution of heat flux,etc.Two sets of probe arrays including 274 probe tips were placed at two ports(approximately 180°separated toroidally),and the spatial and temporal resolutions of this measurement system could reach6 mm and 1μs,respectively.A novel design of the ceramic isolation ring can ensure reliable electrical insulation property between the graphite tip and the copper substrate plate where plasma impurities and the dust are deposited into the gaps for a long experimental time.Meanwhile,the condition monitoring and mode conversion between single and triple probe of the probe system could be conveniently implemented via a remote-control station.The preliminary experimental result shows that the divertor Langmuir probe system is capable of measuring the high spatiotemporal parameters involved the plasma density,electron temperature,particle flux as well as heat flux during the ELMy H-mode discharges.展开更多
基金supported by the Natural Science Foundation of Hubei Province (Nos.2023AFB376 and 2024AFD287)National Key Research and Development Program (No.2023YFC3503804)the National Natural Science Foundation of China (No.22077044)。
文摘Sulfur dioxide(SO_(2)) and its derivatives have been recognized as harmful environmental pollutants.However,they are often produced during the processing of traditional Chinese medicines,potentially compromising the quality of these medicinal materials and contributing to various health issues.Due to a lack of effective monitoring and imaging tools,the physiological effects of excessive SO_(2) residues in traditional Chinese medicine remain unclear.Therefore,developing a rapid and effective tool for detecting SO_(2) is crucial for understanding its metabolic pathways and effects in vivo.In this study,we developed a near infrared(NIR) and ratiometric fluorescent probe,NIR-RS,which exhibits high sensitivity,selectivity,and rapid response for SO_(2) detection.Notably,NIR-RS accurately quantifies SO_(2) contents in Pinelliae rhizoma(P.rhizoma) samples,with recovery rates from 98.46 % to 102.40 %,and relative standard deviations(RSDs)< 5.0 %.For bioimaging applications,NIR-RS has low cytotoxicity and good mitochondrial-targeting ability,making it suitable for imaging exogenous and endogenous SO_(2) in mitochondria.Additionally,NIR-RS was successfully applied to image SO_(2) content of P.rhizoma samples within cells,revealing that high SO_(2) residue elevated mitochondria adenosine triphosphate(ATP) content,these findings reveal that P.rhizoma with excessive SO_(2) can affect the organism's growth mechanisms through alterations in ATP pathways.In vivo,SO_(2) was found to predominantly accumulate in the liver following gavage with P.rhizoma solution,with accumulation levels increasing in proportion to SO_(2) residue concentration.High SO_(2) concentrations in P.rhizoma can cause pulmonary fibrosis and gastric mucosal damage.This work provides a valuable tool for regulating SO_(2) content in P.rhizoma and may help researcher better understand the metabolism of SO_(2) derivatives and explore their physiological roles in biological systems.
文摘A zinc sulfate open framework matrix,[Zn(SO_4)(DMSO)](1),was synthesized by solvothermal evaporationusing dimethyl sulfoxide(DMSO)as the solvent.A compositeP@1,which exhibits fluorescence and room tempera-ture phosphorescence(RTP)properties,was prepared by doping 2,6-naphthalic acid(P)into matrix1at a low con-centration.P@1emitted a green RTP that was visible to the naked eye and lasted for approximately 2 s.P@1exhib-ited selective phosphorescence enhancement response towards Pb^(2+),with a detection limit of 2.52μmol·L^(-1).Themain detection mechanism is the Pb—O coordination-induced phosphorescence enhancement in the system.Inter-estingly,P@1also functioned as a dual-channel probe for the rapid detection of Fe^(3+)ions through fluorescencequenching with a detection limit of 0.038μmol·L^(-1).The recognition mechanism may be attributed to the competi-tive energy absorption betweenP@1and Fe^(3+)ions.CCDC:2388502,1.
基金the financial support from the National Natural Science Foundation of China(Nos.22477101,22277098 and 22037002)the Natural Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China(No.2020JC-38)the Shaanxi Fundamental Science Research Project for Chemistry and Biology(No.22JHQ070)。
文摘Polarity,as a crucial environmental characteristic,plays a significant role in numerous cellular physiological processes.Abnormal changes in polarity are closely associated with various diseases.However,existing tools still have certain limitations that hinder accurate detection of polarity.Therefore,there is a pressing need to develop powerful tools for precisely monitoring changes in polarity.In this study,we developed two dual-emissive fluorogenic dyes by innovatively introducing 1,3-dithio-2-heteroarsenic cyclopentane and 1,2-diselenocyclopentane respectively into the near-infrared(NIR)coumarin-benzopyranium skeleton to enhance their cellular uptake capability.Additionally,we synthesized the polarity-sensitive dual-emissive fluorogenic probe CSFNS,which exhibits high cellular uptake rate,by modifying the spironolactone(Aldactone)structure of CBA into spirolactam.CSFNS not only demonstrates excellent polarity sensitivity in vitro but is also successfully applied to visually monitor the polarity changes in various types of living cells,including healthy cells,cancer cells and drug-induced senescent cells.
文摘以三苯胺衍生物为原料,通过引入不同苯胺衍生物,构建了4种席夫碱荧光探针(FY1—FY4)用于Zn^(2+)的检测,其结构均用1 H NMR进行了表征.实验结果表明,探针FY1—FY4对Zn^(2+)都显示出特异性响应及良好的抗干扰能力.当Zn^(2+)浓度在一定范围内时,探针FY1—FY4的荧光强度与Zn^(2+)浓度均呈良好的线性关系.其中,探针FY2的检测阈值最低,为1.15μmol/L.Job's Plot实验表明,探针FY1—FY4与Zn^(2+)配位的化学计量比约为2∶1.
基金supported by the National Natural Science Foundation of China(No.90713009)
文摘A novel fluorescent Zn^2＋ probe based on 6-methoxyquinolin was synthesized in four steps from inexpensive starting materials. It exhibits very strong fluorescence responses and has a remarkably high selectivity to Zn^2＋ than other physiological relevant metal ions. This new compound could be used as low-priced yet high-quality Zn^2＋ probe.
基金the National Natural Science Foundation of China(No.21276251)Ministry of Human Resources and Social Security of PRC+1 种基金the 100 talents program funded by Chinese Academy of SciencesState Key Laboratory of Fine Chemicals of China(No.KF1105)
文摘In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with excellent selectivity. The reaction mechanism was explained by means of absorption, fluorescence and HPLC-MS.
基金the National Natural Science Foundation of China(Nos.21672185 and 21867019)the"Youth Talent of WanRen Project"Foundation of Yunnan Province of China for the financial support"LianDa Scholar Project"and"Graduate Research and Innovation"Foundation of Yunnan Normal University(No.Ysdyjs2019122)。
文摘Iron is one of the essential trace elements in the human body.It plays an important role in human biology and pathology.Deregulation of iron levels in cells is associated with disease development.In this work,we synthesized a novel near-infrared intramolecular charge transfer(ICT)based ratiometric fluorescent probe to detect Fe^(2+),by using naphthalimide and indole moieties as building blocks.Our work showed that the radiometric probe has excellent selectivity,sensitivity and rapid response.Moreover,we could successfully perform real-time monitoring of Fe^(2+) in He La cells and C.elegans.
基金support from the Natural Science Foundation of Shandong Province(No.ZR2021 MB075)National Natural Science Foundation of China(No.51602297)the Opening Fund of State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical Engineering(No.2021-K53).
文摘The mercury species in the ocean(MeHg,Hg^(2+))will be enriched in marine organisms and threaten human health through the food chain.While the excessive H_(2)O_(2)in the metabolic process will produce hydroxyl radicals and accelerate the aging of human cells,causing a series of diseases.Hence,the cost-effective and rapid detection of mercury and H_(2)O_(2)is of urgent requirement and significance.Here,we synthesized emerging graphitic carbon nitride quantum dots(g-CNQDs)with high fluorescence quantum yield(FLQY)of 42.69%via a bottom-up strategy by a facile one-step hydrothermal method.The g-CNQDs can detect the H_(2)O_(2)and Hg^(2+)through the fluorescence quenching effect between g-CNQDs and detected substances.With the presence of KI,g-CNQDs show concentration-dependent fluorescence toward H_(2)O_(2),with a wide detection range of 1–1000μmolL^(-1)and a low detection limit of 0.23μmolL^(-1).The g-CNQDs also show sensitivity toward Hg^(2+)with a detection range of 0–0.1μmolL^(-1)and a detection limit of 0.038μmolL^(-1).This dual-function detection of g-CNQDs has better practical application capability compared to other quantum dot detection.This study may provide a new strategy for g-CNQDs preparation and construct a fluorescence probe that can be used in various systems involving H_(2)O_(2)and Hg^(2+),providing better support for future bifunctional or multifunction studies.
基金We are grateful for the financial supports from the National Natural Science Foundation of China(Nos.U1704161,U1504203,21601158)Zhengzhou University(No.32210431).
文摘Based on 4-bromo-1,8-naphthalic anhydride,one novel ratiometric fluorescence H_(2)S-probe(IDNA)was designed and synthesized.Further studies indicate that IDNA can sensitively recognize H_(2)S(detection limit of 7 μmol/L)with good selectivity and anti-interference ability.In addition,IDNA has satisfactory photostability in HeLa cells,ability of mitochondrial co-localization,and can be utilized in fluorescence imaging of H_(2)S.
文摘We have developed a simple method for the preparation of highly fluorescent and stable, water-soluble CdTe quantum dots in sol-gel-derived composite silica spheres which were coated with calix[6]arene. The resulting nanoparticles (NPs) were characterized in terms of UV, fluorescence and FT-IR spectroscopy and TEM. The results show that the new NPs display more intense fluorescence intensity and are more stable than its precursors of the type SiO2/CdTe. Under the optimum, the novel NPs exhibit a higher selectivity and ultrasensitive fluorescence probes for the determination of gly-phosate over other pesticides, the fluorescence intensity increase with the concentration of glyphosate in the range from 1.0 to 25.0 nmol/L and the detection limit is low to 0.0725 nmol/L. A mechanism is suggested to explain the inclusion process by a Langmuir binding isotherm.
基金financial support by the National Natural Science Foundation of China (Nos. 21190033, 21372083, 21572039)National 973 Program (No. 2013CB733700)
文摘Most reported fluorescent probes have limitations in practical applications in living systems due to the strong autofluorescence background,construction of probes with near-infrared（NIR） fluorescence emission is an accessible approach for addressing this challenge.We here designed a NIR fluorescent probe for monitoring the endogenous production of H2S in living cells.The designed probe showed significant NIR fluorescence turn-on response to H2S with high selectivity,enabling the sensitive detection H2S.Importantly,the probe could be applied in monitoring the endogenous production of H2S in raw 264.7 macrophages.This study showed that fluvastatin can promote the activity of cystathionineγ-lyase（CSE） for generation H2S.
基金the National Natural Science Foundation of China(Nos.81672508,21675085)Jiangsu Provincial Foundation for Distinguished Young Scholars(Nos.BK20170041,BK20170042)+2 种基金Natural Science Foundation of Shaanxi Province(No.2019JM-016)China-Sweden Joint Mobility Project(No.51811530018)Fundamental Research Funds for the Central Universities。
文摘H2S is an essential gas signal molecule in cells,and viscosity is a key internal environmental parameter.Recent studies have shown that H_(2)S acts as a cytoarchitecture agent and gas transmitter in many tissues,e.g.,as a regulator of neuroendocrine in the brain for mediating vascular tone in blood vessels.Mitochondrial viscosity is an important parameter for judging whether mitochondrial function is normal.It has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson’s disease(PD),and the protective role of H_(2)S in PD models has been extensively demonstrated.Herein,Mito-HS,a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H_(2)S content.Moreover,this probe could detect the relative amount of and changes in mitochondrial H2S in situ due to the reduced mitochondrial targeting ability after reaction with H_(2)S.The results show that H2S in mitochondria is inversely related to viscosity.The PD model has a lower H2S in mitochondria and a higher mitochondrial viscosity than did the normal.This result is important for our deep understanding of PD and its causes.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11225421,11474277,11434010 and 11574305the National Young 1000 Talent Plan
文摘The fundamental momentum conservation requirement q - 0 for the Raman process is relaxed in the nanocrystal- lites （NCs）, and phonons away from the Brillouin-zone center will be involved in the Raman scattering, which is well-known as the phonon confinement effect in NCs. This usually gives a downshift and asymmetric broadening of the Raman peak in various NCs. Recently, the A1 mode of 1L MoS2 NCs is found to exhibit a blue shift and asymmetric broadening toward the high-frequency side [Chem. Soc. Rev. 44 （2015） 2757 and Phys. Rev. B 91 （2015） 195411]. In this work, we carefully check this issue by studying Raman spectra of lL MoS2 NCs prepared by the ion implantation technique in a wide range of ion-implanted dosage. The same confinement coefficient is used for both E＇ and A＇1 modes in 1L MoS2 NCs since the phonon uncertainty in an NC is mainly determined by its domain size. The asymmetrical broadening near the A＇1 and E＇ modes is attributed to the appearance of defect-activated phonons at the zone edge and the intrinsic asymmetrical broadening of the two modes, where the anisotropy of phonon dispersion curves along Г-K and Г- M is also considered. The photoluminescence spectra confirm the formation of small domain size of 1L MoS2 nanocrystallites in the ion-implanted 1L MoS2. This study provides not only an approach to quickly probe phonon dispersion trends of 2D materials away from Г by the Raman scattering of the corresponding NCs, but also a reference to completely understand the confinement effect of different modes in various nanomaterials.
基金Supported by the National Natural Science Foundation of China(No.20771082)the Natural Science Foundation of Tianjin Municipality, China(No.09JCYBJC05800)the Educational Commission of Tianjin Municipality, China (No.20080509)
文摘Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.
文摘By means of total energy calculations within the framework of the local density approximation (LDA), the interactions between a silicon Si(001) surface and a scanning probe are investigated. The tip of the probe, comprising 4 Si atoms scans along the dimer lines above an asymmetric p(2 × 1) surface, at a distance where the chemical interaction between tip-surface is dominant and responsible for image resolution. At that distance, the tip causes the dimer to toggle when it scans above the lower atom of a dimer. The toggled dimers create an alternating pattern, where the immediately adjacent neighbours of a toggled dimer remain unchanged. After the tip has fully scanned across the p(2 × 1) surface, causes the dimers to arrange in a p(2 × 2) reconstruction, reproducing the images obtained in scanning probe experiments. Our modelling methodology includes simulations that reveal the energy input required to overcome the barrier to the onset of dimer toggling. The results show that the energy input to overcome this barrier is lower for the p(2 × 1) surface than that for the p(2 × 2) or c(4 × 2) surfaces.
基金the National Natural Science Foundation of China(NSFC,Nos.92159201,81630045 and 81927802 to F.WangNo.81971676 to J.Shi+3 种基金No.32027801 to Z.Hu)National Key R&D Program of China(No.2017YFA0205600 to F.Wang)Emergency Key Program of Guangzhou Laboratory,(No.EKPG21–16 to F.Wang)Youth Innovation Promotion Association of Chinese Academy of Sciences(YIPACAS,No.2016090 to J.Shi)for financial support。
文摘Tumor-related PD-L2 expression is associated with the clinical efficacy of PD-1/PD-L1 blockade therapy.PD-L2-specific imaging can help selecting patients for appropriate immunotherapy.In this study,a PD-L2-targeting peptide(PDP2)was screened by the one-bead one-compound combinatorial library approach.Using the retro-inverso D-peptide of PDP2(RD-PDP2)and PEGylation strategies,we developed a novel Tc-99m-labeled PD-L2-targeting peptide as a SPECT tracer(^(99m)Tc-PEG_(6)-RD-PDP2)for imaging of tumor PD-L2 expression.The radiolabeling yield of ^(99m)Tc-PEG_(6)-RD-PDP2 was greater than 95%by the standard HYNIC/tricine/TPPTS labeling procedure.^(99m)Tc-PEG_(6)-RD-PDP2 displayed high PD-L2-binding specificity both in vitro and in vivo.SPECT/CT imaging with^(99m)Tc-PEG_(6)-RD-PDP2 showed that the A549-PD-L2tumors were clearly visualized,whereas the signals in PD-L2-negative A549 tumors were much lower.In vivo blocking study suggested that the tumor uptake of^(99m)Tc-PEG_(6)-RD-PDP2 was PD-L2 specifically mediated.^(99m)Tc-PEG_(6)-RD-PDP2 is a promising SPECT probe for the non-invasive imaging of tumor PD-L2expression and has a great potential in guiding the anti-PD-1 or anti-PD-L1 immunotherapy of cancer.
基金financially supported by the National Natural Science Foundation of China(No.21877082)the International Science and Technology Innovation Cooperation Project of Sichuan Province(No.2021YFH0132)+1 种基金the Sichuan Science and Technology Program(No.2021YFG0291)the Undergraduate Scientific and Technological Innovation Project(Nos.2021127,2021130),Xihua University。
文摘As a strong oxidizer,hypochlorite(Cl O^(-))are widely employed as bleaching agents and disinfectants.Determination of ClO^(-)is required to ensure bactericidal effects and avoid hazards caused by excessive residual chlorine.Herein,the derivative bicyclic 2-pyridone,namely DHIP-Py,was prepared successfully to establish a new ClO^(-)-quantitative method.The probe exhibits excellent Cl O^(-)selectivity over other ROS and anions/cations,high sensitivity(LOD=1.32μmol/L),fast response(<5 s),and wide-p H tolerance(pH4~10).Benefit from its good water solubility,DHIP-Py is well suited for water sample analysis and has been successfully applied to detect Cl O^(-)in real-world food and environmental samples,including tap water,bottled water and river water.The detection results were essentially identical to that of obtained from traditional DPD method.Moreover,visual detection of Cl O^(-)via filter paper-based solid sensor and imaging of ClO^(-)in Escherichia coli were also achieved by DHIP-Py.These satisfactory results demonstrate that this bicyclic 2-pyridone-based hypochlorite probe is a promising free chlorine chemosensor with great potential for analytical applications.
基金partially supported by National Natural Science Foundation of China(Nos.11875017,11875020,12175186 and 11905052)the National Magnetic Confinement Fusion Science Program of China(Nos.2019YFE03030002,2017YFE0301203 and 2018YFE0310100)the Sichuan Outstanding Youth Science Foundation(No.2020JDJQ0019)。
文摘A newly designed divertor Langmuir probe diagnostic system has been installed in a rare closed divertor of the HL-2A tokamak and steadily operated for the study of divertor physics involved edge-localized mode mitigation,detachment and redistribution of heat flux,etc.Two sets of probe arrays including 274 probe tips were placed at two ports(approximately 180°separated toroidally),and the spatial and temporal resolutions of this measurement system could reach6 mm and 1μs,respectively.A novel design of the ceramic isolation ring can ensure reliable electrical insulation property between the graphite tip and the copper substrate plate where plasma impurities and the dust are deposited into the gaps for a long experimental time.Meanwhile,the condition monitoring and mode conversion between single and triple probe of the probe system could be conveniently implemented via a remote-control station.The preliminary experimental result shows that the divertor Langmuir probe system is capable of measuring the high spatiotemporal parameters involved the plasma density,electron temperature,particle flux as well as heat flux during the ELMy H-mode discharges.
