The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration re...The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration remain elusive. In this study, we investigated the expression and possible functions of the SoxC genes after retinal and optic nerve injury in adult zebrafish. We found that among the five SoxC members, Soxllb was strongly induced in BrdU-positive cells in the inner nuclear layer （INL） after retinal injury, and morpholino-mediated Soxllb-knockdown significantly reduced the number of proliferating cells in the INL at 4 days post-injury. After optic nerve lesion, both Soxl la and Soxl lb were strongly expressed in retinal ganglion cells （RGCs）, and knockdown of both Soxl la and Soxllb inhibited RGC axon regrowth in retinal explants. Our study thus uncovered a novel expression pattern of SoxC family genes after retinal and optic nerve injury, and suggests that they have important functions during retinal and optic nerve regeneration.展开更多
Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of th...Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches,suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation,they are expressed in the neural ectoderm and archenterons. In adults,they are expressed not only in the nerve cord,but also in the gut,midgut diverticulum,gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.展开更多
基金supported by grants from National Natural Science Foundation of China(31401234)Natural Science Foundation of Jiangsu Province,China(BK20140428)+1 种基金the Basic Research Program of Education Department of Jiangsu Province,China(14KJB180019)the Science and Technology Project of Nantong Municipality,Jiangsu Province,China(MS22015002)
文摘The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration remain elusive. In this study, we investigated the expression and possible functions of the SoxC genes after retinal and optic nerve injury in adult zebrafish. We found that among the five SoxC members, Soxllb was strongly induced in BrdU-positive cells in the inner nuclear layer （INL） after retinal injury, and morpholino-mediated Soxllb-knockdown significantly reduced the number of proliferating cells in the INL at 4 days post-injury. After optic nerve lesion, both Soxl la and Soxl lb were strongly expressed in retinal ganglion cells （RGCs）, and knockdown of both Soxl la and Soxllb inhibited RGC axon regrowth in retinal explants. Our study thus uncovered a novel expression pattern of SoxC family genes after retinal and optic nerve injury, and suggests that they have important functions during retinal and optic nerve regeneration.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 30700434 and 30671072)the Natural Science Foundation of Shandong Province (Grant No. Y2007D20)+1 种基金the Shandong Provincial Scientific Research Foundation for Excellent Young Scientists (Grant No. 2006BS02009)the National Basic Re-search and Development Program of China (Grant Nos. 2007CB815800 and 2007CB947100)
文摘Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches,suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation,they are expressed in the neural ectoderm and archenterons. In adults,they are expressed not only in the nerve cord,but also in the gut,midgut diverticulum,gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.
