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浙江省桃花水母18S rRNA基因序列分析及物种鉴定 被引量:10
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作者 徐善良 周剑君 +1 位作者 王丹丽 孙雪 《淡水渔业》 CSCD 北大核心 2010年第2期14-18,共5页
采用PCR和DNA测序技术扩增和测序了2003-2008年间采自浙江省宁波、杭州、温州和金华4个地区7个地点桃花水母(Craspedacusta)核糖体小亚基rRNA(18S rRNA)基因,并与已知索氏桃花水母(Craspedacusta sow-erbyi)和中华桃花水母(Crasp... 采用PCR和DNA测序技术扩增和测序了2003-2008年间采自浙江省宁波、杭州、温州和金华4个地区7个地点桃花水母(Craspedacusta)核糖体小亚基rRNA(18S rRNA)基因,并与已知索氏桃花水母(Craspedacusta sow-erbyi)和中华桃花水母(Craspedacusta sinensis)18S rRNA基因序列进行了比对。结果显示:这7个地点的桃花水母与索氏桃花水母序列极为相似,相似度达99.67%-100%,遗传距离为0-0.002,而与同属中华桃花水母的遗传距离为0.013-0.015。分子系统学分析显示这7个地点桃花水母与索氏桃花水母分支的置信度高达100%。结果确定分析样品均为索氏桃花水母。 展开更多
关键词 索氏桃花水母(Craspedacusta sowerby) 18SrRNA 分类学
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Karyotypes of three species of the marine Veneroida molluscs 被引量:4
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作者 Sun Zhenxing1, Shao Yanqun1, Guo Shengchao1, Qin Yan1, Yang Aiguo2 1. Department of Biological Science and Biotechnology, Yantai Normal University, Yantai 264025, China. 2. Yellow Sea Fisheries Research Institute, Qingdao 266071, China. 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2003年第4期671-678,共8页
The karyotypes of three species of marine Veneroida molluscs, Solen grandis Dunker, Saxidomus purpuratus Sowerby and Mactra chinensis Philippi were studied by using the adult gill tissues. The chromosomes were-prepare... The karyotypes of three species of marine Veneroida molluscs, Solen grandis Dunker, Saxidomus purpuratus Sowerby and Mactra chinensis Philippi were studied by using the adult gill tissues. The chromosomes were-prepared through injecting phytohemagglutinin (PHA) and colchicine, hypotonic treatment, chopping air-dry, and squashing technology. The results show that the diploid chromosome numbers of the three Veneroida species are the same as 2n = 38. The karyotype of Solen grandis is 26m + 6sm + 2st + 4t, NF = 70, the karyotype of Saxidomus purpuratus is 32m + 2sm + 4st/t, NF = 72, and the karyotype of Mactra chinensis is 20m + 16sm + 2st/t, NF = 74. Satellite and sex chromosome were not found among the chromosomes of three species. 展开更多
关键词 Solen grandis Dunker Saxidomus purpuratus sowerby Mactra chinensis Philippi CHROMOSOME KARYOTYPE
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