OBJECTIVE: To explore whether Soufeng Yuchuan(搜风愈喘, SFYC) formula alleviates asthma by promoting ferroptosis of airway epithelial cells. METHODS: A chronic asthma rat model was established using ovalbumin(OVA) com...OBJECTIVE: To explore whether Soufeng Yuchuan(搜风愈喘, SFYC) formula alleviates asthma by promoting ferroptosis of airway epithelial cells. METHODS: A chronic asthma rat model was established using ovalbumin(OVA) combined with aluminum hydroxide sensitization and OVA nebulization stimulation. Lung tissue pathology was assessed via hematoxylin and eosin staining and periodic acid-Schiff staining. Lung tissue ultrastructure was observed using transmission electron microscopy. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. Malondialdehyde(MDA), glutathione, and tissue ferrous ion content in rat lung tissues and cells were detected using commercial kits. Immunohistochemical staining was used to determine the expression levels of interleukin-13(IL-13) and interleukin-6(IL-6) in lung tissue. Western blotting was employed to detect the expression levels of transferrin receptor 1(TFR1), transferrin receptor 2(TFR2), acyl-Co A synthetase longchain family member 4(ACSL4), glutathione peroxidase 4(GPX4), and solute carrier family 7 member 11(SLC7A11). Cell proliferation, apoptosis and reactive oxygen species(ROS) were evaluated using a cell counting kit-8 assay and flow cytometry. Intracellular lipid peroxidation was detected using a C11-BODIPY 581/591(C11-BODIPY) probe. RESULTS: SFYC formula effectively alleviates airway inflammation in asthmatic rats in a dose-dependent manner. It improves airway epithelial cell integrity, reduces alveolar wall thickening and expansion, inhibits goblet cell proliferation and mucus secretion, and decreases the expression of IL-6 and IL-13 in lung tissue, as well as the serum levels of IL-6, IL-13, interleukin-4(IL-4), and immunoglobulin E(Ig E). SFYC formula mitigates ferroptosis in lung tissue of asthmatic rats. At the cellular level, it promotes proliferation of rat airway epithelial cells, inhibits cell apoptosis, suppresses ROS, MDA, and lipid accumulation, reduces the expression of TFR1, TFR2, and ASCL4, and increases the expression of GPX4 and SLC7A11 to mitigate ferroptosis. CONCLUSION: SFYC formula alleviates asthma airway inflammation by inhibiting ferroptosis in airway epithelial cells, providing new insights and potential therapeutic targets for the development of new asthma treatment drugs.展开更多
Objective:To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis.Methods:Mice of six weeks old which were knocked out gene ApoE and fed with a high-fat diet...Objective:To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis.Methods:Mice of six weeks old which were knocked out gene ApoE and fed with a high-fat diet for 13 weeks to establish a model of atherosclerotic unstable plaque,and they were randomly divided into the model group,the atorvastatin group and the low,medium,and high-dose Shoufeng Qutan groups.In addition,six weeks old C57BL/6J mice were set as the blank con?trol group. Both the blank control group and the model group were given 0.9% normal saline by gavage. The low,medium,and high-dose groups of Soufeng Qutangroup and the atorvastatin group were given different doses of Soufeng Qutan Chinese Medicine Decoction and atorvastatin. After 13 weeks,the mice were sacrificed,and the abdominal aortic blood vessels were observed by HE staining. Total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C). and high-density lipopro?tein cholesterol(HDL-C)content were detected by a biochemical automatic analyzer and IL-1β levels were detected by ELISA.Results:Compared with the blank control group,a large number of foam cells could be seen in the model group with obvious plaques;serum TC,TG,LDL-C,and IL-1β levels were significantly increased(P<0.05),and HDL-C level was markedly de?creased (P<0.05). Compared with the model group,the Soufeng Qutangroup at all doses and the atorvastatin group had a small amount of foam cells and reduced plaque area;serum TC,TG,LDL-C,and IL-1β levels were significantly decreased (P<0.05),and the level of HDL-C was significantly increased (P<0.05).Conclusion:Soufeng Qutan Chinesemedicine can inhibit the pro?gression of cellular inflammation,downregulate the expression of IL-1β,reduce plaques,and improve blood lipids. This may be one of the molecular mechanisms of Soufeng Qutan Chinese medicine in the early treatment of atherosclerosis.展开更多
基金Supported by Natural Science Foundation-funded Project:the Study on the Mechanism of Soufeng Yuchuan Formula in Regulating Airway Remodeling in Asthmatic Rats Based on Epithelial-Mesenchymal Transition(No. 82174438)。
文摘OBJECTIVE: To explore whether Soufeng Yuchuan(搜风愈喘, SFYC) formula alleviates asthma by promoting ferroptosis of airway epithelial cells. METHODS: A chronic asthma rat model was established using ovalbumin(OVA) combined with aluminum hydroxide sensitization and OVA nebulization stimulation. Lung tissue pathology was assessed via hematoxylin and eosin staining and periodic acid-Schiff staining. Lung tissue ultrastructure was observed using transmission electron microscopy. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. Malondialdehyde(MDA), glutathione, and tissue ferrous ion content in rat lung tissues and cells were detected using commercial kits. Immunohistochemical staining was used to determine the expression levels of interleukin-13(IL-13) and interleukin-6(IL-6) in lung tissue. Western blotting was employed to detect the expression levels of transferrin receptor 1(TFR1), transferrin receptor 2(TFR2), acyl-Co A synthetase longchain family member 4(ACSL4), glutathione peroxidase 4(GPX4), and solute carrier family 7 member 11(SLC7A11). Cell proliferation, apoptosis and reactive oxygen species(ROS) were evaluated using a cell counting kit-8 assay and flow cytometry. Intracellular lipid peroxidation was detected using a C11-BODIPY 581/591(C11-BODIPY) probe. RESULTS: SFYC formula effectively alleviates airway inflammation in asthmatic rats in a dose-dependent manner. It improves airway epithelial cell integrity, reduces alveolar wall thickening and expansion, inhibits goblet cell proliferation and mucus secretion, and decreases the expression of IL-6 and IL-13 in lung tissue, as well as the serum levels of IL-6, IL-13, interleukin-4(IL-4), and immunoglobulin E(Ig E). SFYC formula mitigates ferroptosis in lung tissue of asthmatic rats. At the cellular level, it promotes proliferation of rat airway epithelial cells, inhibits cell apoptosis, suppresses ROS, MDA, and lipid accumulation, reduces the expression of TFR1, TFR2, and ASCL4, and increases the expression of GPX4 and SLC7A11 to mitigate ferroptosis. CONCLUSION: SFYC formula alleviates asthma airway inflammation by inhibiting ferroptosis in airway epithelial cells, providing new insights and potential therapeutic targets for the development of new asthma treatment drugs.
基金Distinguished Professor Project of Liaoning Province([2018]No.35)。
文摘Objective:To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis.Methods:Mice of six weeks old which were knocked out gene ApoE and fed with a high-fat diet for 13 weeks to establish a model of atherosclerotic unstable plaque,and they were randomly divided into the model group,the atorvastatin group and the low,medium,and high-dose Shoufeng Qutan groups.In addition,six weeks old C57BL/6J mice were set as the blank con?trol group. Both the blank control group and the model group were given 0.9% normal saline by gavage. The low,medium,and high-dose groups of Soufeng Qutangroup and the atorvastatin group were given different doses of Soufeng Qutan Chinese Medicine Decoction and atorvastatin. After 13 weeks,the mice were sacrificed,and the abdominal aortic blood vessels were observed by HE staining. Total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C). and high-density lipopro?tein cholesterol(HDL-C)content were detected by a biochemical automatic analyzer and IL-1β levels were detected by ELISA.Results:Compared with the blank control group,a large number of foam cells could be seen in the model group with obvious plaques;serum TC,TG,LDL-C,and IL-1β levels were significantly increased(P<0.05),and HDL-C level was markedly de?creased (P<0.05). Compared with the model group,the Soufeng Qutangroup at all doses and the atorvastatin group had a small amount of foam cells and reduced plaque area;serum TC,TG,LDL-C,and IL-1β levels were significantly decreased (P<0.05),and the level of HDL-C was significantly increased (P<0.05).Conclusion:Soufeng Qutan Chinesemedicine can inhibit the pro?gression of cellular inflammation,downregulate the expression of IL-1β,reduce plaques,and improve blood lipids. This may be one of the molecular mechanisms of Soufeng Qutan Chinese medicine in the early treatment of atherosclerosis.
