Three DNA regions were sequenced for testing six fresh plant samples of Salvia species.These three DNA regions were nrDNA ITS(nuclear ribosomal DNA internal transcribed spacer),chloroplast rp116(the gene encoding ribo...Three DNA regions were sequenced for testing six fresh plant samples of Salvia species.These three DNA regions were nrDNA ITS(nuclear ribosomal DNA internal transcribed spacer),chloroplast rp116(the gene encoding ribosomal protein L16),and trnL-trnF(the cpDNA region comprising the trnL and the intergenic spacer between trnL and trnF).The results showed that the nrDNA ITS and rp116 genes could provide novel information for origin identification of Salvia species.Due to their higher mutation rates of these 2 gene markers,Salvia species-specific primers were designed and S.miltiorrhiza and S.yuwumensis were identified.The trnL-trnF gene expressed low mutation rote,it could not identify the species.Since the damage of DNA by the pretreatments of the dry roots of Chinese herbs,it is hard to apply the molecular markers to commercial samples for identification.展开更多
基金Pharmaceutical Innovation and Traditional Chinese Medicine Modernization Program of China(2004AA2Z3342)
文摘Three DNA regions were sequenced for testing six fresh plant samples of Salvia species.These three DNA regions were nrDNA ITS(nuclear ribosomal DNA internal transcribed spacer),chloroplast rp116(the gene encoding ribosomal protein L16),and trnL-trnF(the cpDNA region comprising the trnL and the intergenic spacer between trnL and trnF).The results showed that the nrDNA ITS and rp116 genes could provide novel information for origin identification of Salvia species.Due to their higher mutation rates of these 2 gene markers,Salvia species-specific primers were designed and S.miltiorrhiza and S.yuwumensis were identified.The trnL-trnF gene expressed low mutation rote,it could not identify the species.Since the damage of DNA by the pretreatments of the dry roots of Chinese herbs,it is hard to apply the molecular markers to commercial samples for identification.
