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Microbial community structure and functional metabolic diversity are associated with organic carbon availability in an agricultural soil 被引量:6
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作者 LI Juan LI Yan-ting +3 位作者 YANG Xiang-dong ZHANG Jian-jun LIN Zhi-an ZHAO Bing-qiang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第12期2500-2511,共12页
Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental ... Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included: a non-amended control(CK), a commonly used application rate of inorganic fertilizer treatment(NPK); a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment(NPKM), and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment(NPKS). Denaturing gradient gel electrophoresis(DGGE) of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term(NPKM, NPKS) significantly promoted soil bacterial structure than the application of inorganic fertilizer only(NPK), and NPKM treatment was the most important driver for increases in the soil microbial community richness(S) and structural diversity(H). Overall utilization of carbon sources by soil microbial communities(average well color development, AWCD) and microbial substrate utilization diversity and evenness indices(H' and E) indicated that long-term inorganic fertilizer with organic amendments incorporated(NPKM, NPKS) could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis(PCA) based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis(RDA) indicated that soil organic carbon(SOC) availability, especially soil microbial biomass carbon(Cmic) and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China's soil resource. 展开更多
关键词 long-term fertilization regimes organic amendment soil microbial community structure microbial functional metabolic activity carbon substrate utilization
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On the Total Dynamic Response of Soil-Structure Interaction System in Time Domain Using Elastodynamic Infinite Elements with Scaling Modified Bessel Shape Functions
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作者 Konstantin Kazakov 《American Journal of Computational Mathematics》 2013年第2期104-109,共6页
This paper is devoted to a new approach—the dynamic response of Soil-Structure System (SSS), the far field of which is discretized by decay or mapped elastodynamic infinite elements, based on scaling modified Bessel ... This paper is devoted to a new approach—the dynamic response of Soil-Structure System (SSS), the far field of which is discretized by decay or mapped elastodynamic infinite elements, based on scaling modified Bessel shape functions are to be calculated. These elements are appropriate for Soil-Structure Interaction problems, solved in time or frequency domain and can be treated as a new form of the recently proposed elastodynamic infinite elements with united shape functions (EIEUSF) infinite elements. Here the time domain form of the equations of motion is demonstrated and used in the numerical example. In the paper only the formulation of 2D horizontal type infinite elements (HIE) is used, but by similar techniques 2D vertical (VIE) and 2D corner (CIE) infinite elements can also be added. Continuity along the artificial boundary (the line between finite and infinite elements) is discussed as well and the application of the proposed elastodynamical infinite elements in the Finite element method is explained in brief. A numerical example shows the computational efficiency and accuracy of the proposed infinite elements, based on scaling Bessel shape functions. 展开更多
关键词 soil-structure Interaction Wave Propagation INFINITE Elements FINITE Element Method BESSEL functions DUHAMEL INTEGRAL
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Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity 被引量:9
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作者 XIE Xiaomei LIAO Min +1 位作者 MA Aili ZHANG Haijun 《Chinese Journal Of Geochemistry》 EI CAS 2011年第3期366-374,共9页
To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil micro-bial community structural and functional diversities, an incubation experiment was conducted, by employing two soil... To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil micro-bial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids (PLFAs) profile and sole carbon source utilization pattern (BIOLOG) of the samples were tested. The results showed that the compo-sition of the microbial communities changed significantly at different levels of metals application. The principal component analyses (PCA) of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and in-creasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorgan-isms, the functional diversity indices [Richness (S), Shannon-Wiener indices (H) and Evenness (EH)] were signifi-cantly lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg. 展开更多
关键词 微生物群落结构 镉(CD) 功能多样性 污染土壤 重金属污染 土壤微生物群落 多样性指数
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A Modified Model for Soil–Structure Interface Considering Random Damage of Mesoscopic Contact Elements
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作者 KE Li-jun GAO Yu-feng +2 位作者 ZHAO Zi-hao LI Da-yong JI Jian 《China Ocean Engineering》 SCIE EI CSCD 2023年第5期807-818,共12页
The interaction between soil and marine structures like submarine pipeline/pipe pile/suction caisson is a complicated geotechnical mechanism process.In this study,the interface is discretized into multiple mesoscopic ... The interaction between soil and marine structures like submarine pipeline/pipe pile/suction caisson is a complicated geotechnical mechanism process.In this study,the interface is discretized into multiple mesoscopic contact elements that are damaged randomly throughout the shearing process due to the natural heterogeneity.The evolution equation of damage variable is developed based on the Weibull function,which is able to cover a rather wide range of distribution shapes by only two parameters,making it applicable for varying scenarios.Accordingly,a statistical damage model is established by incorporating Mohr–Coulomb strength criterion,in which the interfacial residual strength is considered whereby the strain softening behavior can be described.A concept of“semi-softening”characteristic point on shear stress–displacement curve is proposed for effectively modeling the evolution of strain softening.Finally,a series of ring shear tests of the interfaces between fine sea sand and smooth/rough steel surfaces are conducted.The predicted results using the proposed model are compared with experimental data of this study as well as some results from existing literature,indicating that the model has a good performance in modeling the progressive failure and strain softening behavior for various types of soil–structure interfaces. 展开更多
关键词 soilstructure interface statistical damage model mesoscopic element Weibull function “semi-softening”characteristic point
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Plant functional trait diversity and structural diversity co-underpin ecosystem multifunctionality in subtropical forests 被引量:6
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作者 Shuai Ouyang Mengmeng Gou +8 位作者 Pifeng Lei Yue Liu Liang Chen Xiangwen Deng Zhonghui Zhao Yelin Zeng Yanting Hu Changhui Peng Wenhua Xiang 《Forest Ecosystems》 SCIE CSCD 2023年第2期153-161,共9页
Tree species diversity is assumed to be an important component in managing forest ecosystems because of effects on multiple functions or ecosystem multifunctionality.However,the importance of tree diversity in determi... Tree species diversity is assumed to be an important component in managing forest ecosystems because of effects on multiple functions or ecosystem multifunctionality.However,the importance of tree diversity in determining multifunctionality in structurally complex subtropical forests relative to other regulators(e.g.,soil microbial diversity,stand structure,and environmental conditions)remains uncertain.In this study,effects of aboveground(species richness and functional and structural diversity)and belowground(bacterial and fungal diversity)biodiversity,functional composition(community-weighted means of species traits),stand structure(diameter at breast height and stand density),and soil factors(pH and bulk density)on multifunctionality(including biomass production,carbon stock,and nutrient cycling)were examined along a tree diversity gradient in subtropical forests.The community-weighted mean of tree maximum height was the best predictor of ecosystem multifunctionality.Functional diversity explained a higher proportion of the variation in multifunctionality than that of species richness and fungal diversity.Stand structure-played an important role in modulating the effects of tree diversity on multifunctionality.The work highlights that species composition and maximizing forest structural complexity are effective strategies to increase forest multifunctionality while also conserving biodiversity in the management of multifunctional forests under global environmental changes. 展开更多
关键词 Abiotic and biotic factors BIODIVERSITY functional composition functional traits soil microbial diversity Stand structure
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Effects of enrichmemt planting with native tree species on bacterial community structure and potential impact on Eucalyptus plantations in southern China 被引量:2
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作者 Yuxing Xu Chao Li +3 位作者 Wankuan Zhu Zhichao Wang Lichao Wu Apeng Du 《Journal of Forestry Research》 SCIE CAS CSCD 2022年第4期1349-1363,共15页
Multi-generational planting of Eucalyptus species degrades soil quality but the introduction of legumes can improve soil fertility and microbial diversity.However,the effects of introducing non-legume native tree spec... Multi-generational planting of Eucalyptus species degrades soil quality but the introduction of legumes can improve soil fertility and microbial diversity.However,the effects of introducing non-legume native tree species on soil nutrients and bacterial community structure remain poorly understood.This study investigated the impacts of the conversion of third generation monoculture Eucalyptus plantations to mixed systems including Eucalyptus urograndis with Cinnamomum camphora(EC)and E.urograndis with Castanopsis hystrix(EH),on soil chemical and biochemical properties and bacterial community structure,diversity and functions.First generation E.urophylla plantations were the control.Results show that planting the third generation Eucalyptus led to a significant decrease in p H,organic matter,nutrient content,enzyme activities(invertin,acid phosphataes,and urease),and bacterialα-diversity compare to the controls.However,the mixed planting showed significant improvement in soil chemical and biochemical attributes and bacterialα-diversity,although the E.urograndis and C.hystrix planting had no improvement.Chloroflexi(oligotrophic bacteria)were significantly enriched in third generation Eucalyptus and Eucalyptus+C.hystrix,while proteobacteria increased significantly in the E.urograndis with C.camphora plantings.The relative abundance of multiple metabolic pathways increased significantly in the third generation Eucalyptus plantations whereas membrane transportrelated genes were enriched in soils of the mixed systems.The changes in bacterial community structures in the two mixed systems were driven by diversity,organic matter and acid phosphatase,while bacterial functions were affected by invertase,NO_(3)^(-)-N,diversity and urease.These results suggest that the transformation of successive monoculture Eucalyptus plantations into mixed plantations reduces the depletion of soil nutrients and enhances the ecological function of soil microorganisms. 展开更多
关键词 soil chemical propertie Enzyme activity 16S rRNA amplicon sequencing Bacterial community structure Tax4Fun function prediction
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An improved damaging model for structured clays
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作者 姜岩 雷华阳 +1 位作者 郑刚 徐舜华 《Journal of Central South University》 SCIE EI CAS 2008年第S2期76-80,共5页
An improved damaging model formulated within the framework of bounding surface for structured clays was proposed. The model was intended to describe the effects of structure degradation due to geotechnical loading. Th... An improved damaging model formulated within the framework of bounding surface for structured clays was proposed. The model was intended to describe the effects of structure degradation due to geotechnical loading. The predictive capability of the model was compared with those of triaxial compression test on Tianjin soft clays. The results show that, by incorporating a new damage function into the model, the reduction of elastic bulk and shear modulus with elastic deformations and the reduction of plastic bulk modulus and shear modulus with plastic deformations are able to be appreciable. Before the axial strain reaches 15%, the axial strain computed from the model is smaller than that from the test under the drained condition. Under the undrained condition, after the axial strain reaches 1%, the axial strain increases quickly because of the complete loss of structure and stiffness; and the result computed from the model is nearly equal to that from the model without the incorporation of the damage function due to less plastic strain under undrained condition test. 展开更多
关键词 structureD clays CONSTITUTIVE RELATION soil structure bounding surface DAMAGE function
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中稻单作模式下江汉平原潮土性水稻土结构功能年际变化趋势模拟研究
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作者 刘炜 黄羽翔 +4 位作者 杨添雨 杨显华 董志强 胡泽苏 金慧芳 《农业资源与环境学报》 北大核心 2026年第1期175-185,共11页
农业耕作是影响土壤通气透水、保肥供肥等结构功能的重要措施,本研究旨在揭示长期中稻单作模式下耕层土壤结构变化趋势及其影响因素,为优化耕层构型、促进耕层质量提升和耕地资源合理利用提供参考依据。本研究通过连续耕作的田间原位试... 农业耕作是影响土壤通气透水、保肥供肥等结构功能的重要措施,本研究旨在揭示长期中稻单作模式下耕层土壤结构变化趋势及其影响因素,为优化耕层构型、促进耕层质量提升和耕地资源合理利用提供参考依据。本研究通过连续耕作的田间原位试验,模拟江汉平原潮土性水稻土中稻单作(水耕-空闲)模式下,土壤结构功能指标的年际变化规律。结果表明:长期水耕会导致土壤容重逐年增大、土壤板结。随着水耕年限延长,土壤容重较第1年增幅分别为3.6%(3年)、5.8%(6年)、6.9%(10年);土壤总孔隙度呈降低趋势,较第1年分别降低了3.8%(3年)、5.9%(6年)、7.2%(10年)。随着水耕年限增加,粉粒和黏粒含量先增后减,砂粒含量则表现出相反的变化特征,与第1年比较,土壤砂粒含量有所增加,而黏粒含量则有所降低。土壤团聚程度和持水能力随耕作年限延长呈逐渐降低趋势,其中田间持水量较第1年降幅分别为9.6%(3年)、11.1%(6年)、15.8%(10年)。连续水耕情况下土壤结构功能在第3、第6年和第10年有明显退化表征,说明长期机械水耕对土壤结构有明显破坏作用,水耕3~4年后宜水旱交替耕作(水旱轮作),缓解长期水耕造成的土壤物理退化问题。研究表明,长期水耕导致土壤耕层结构功能退化严重,研究结果为江汉平原适宜耕作模式/种植制度选择、促进区域耕地资源可持续利用提供参数支持和理论依据。 展开更多
关键词 团聚体 连续水耕 结构功能 模拟试验 潮土性水稻土
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Distribution of available soil water capacity in China 被引量:79
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作者 ZHOUWenzuo LIUGaohuan +1 位作者 PANJianjun FENGXianfeng 《Journal of Geographical Sciences》 SCIE CSCD 2005年第1期3-12,共10页
The available soil water capacity (ASWC) is important for studying crop production, agro-ecological zoning, irrigation planning, and land cover changes. Laboratory determined data of ASWC are often not available for m... The available soil water capacity (ASWC) is important for studying crop production, agro-ecological zoning, irrigation planning, and land cover changes. Laboratory determined data of ASWC are often not available for most of soil profiles and the nationwide ASWC largely remains lacking in relevant soil data in China. This work was to estimate ASWC based on physical and chemical properties and analyze the spatial distribution of ASWC in China. The pedo-transfer functions (PTFs), derived from 220 survey data of ASWC, and the empirical data of ASWC based on soil texture were applied to quantify the ASWC. GIS technology was used to develop a spatial file of ASWC in China and the spatial distribution of ASWC was also analyzed. The results showed the value of ASWC ranges from 15 × 10-2 cm3·cm-3 to 22 × 10-2 cm3·cm-3 for most soil types, and few soil types are lower than 15 × 10-2 cm3·cm-3 or higher than 22 × 10-2 cm3·cm-3. The ASWC is different according to the complex soil types and their distribution. It is higher in the east than that in the west, and the values reduce from south to north except the northeastern part of China. The "high" values of ASWC appear in southeast, northeastern mountain regions and Northeast China Plain. The relatively "high" values of ASWC appear in Sichuan basin, Huang-Huai-Hai plain and the east of Inner Mongolia. The relatively "low" values are distributed in the west and the Loess Plateau of China. The "very low" value regions are the northern Tibetan Plateau and the desertified areas in northern China. In some regions, the ASWC changes according to the complex topography and different types of soils. Though there remains precision limitation, the spatial data of ASWC derived from this study are improved on current data files of soil water retention properties for Chinese soils. This study presents basic data and analysis methods for estimation and evaluation of ASWC in China. 展开更多
关键词 available soil water capacity pedo-transfer functions China
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Quantitative Effect of Soil Texture Composition on Retardation Factor of K^+ Transport 被引量:16
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作者 WANGYU ZHANGYIPING 《Pedosphere》 SCIE CAS CSCD 2001年第4期377-382,共6页
With six packed columns composed of < 1 μm and 5 μm~0.25 mm fractions from an Eum-Orthic An- throsol (Columns 1~6) and one column of the Eum-Orthic Anthrosol (Column 7), K~(+) transport experiments under the c... With six packed columns composed of < 1 μm and 5 μm~0.25 mm fractions from an Eum-Orthic An- throsol (Columns 1~6) and one column of the Eum-Orthic Anthrosol (Column 7), K~(+) transport experiments under the condition of saturated steady water flow were conducted to qualify the effects of soil texture com- position on the retardation factor (R) of K~(+) transport. The results showed that the retardation factor of K~ (+) transport in the tested soil columns greatly increased with increasing clay contents. In an attempt to use pedo-transfer function (PTF) approach in the solute transport study, a preliminary PTF was established through the six packed columns (Columns 1~6) with soil basic data including soil bulk density, volumet- ric water content and clay content to predict the retardation factor, and proved valid by the satisfactory prediction of R in Column 7. 展开更多
关键词 pedo-transfer function retardation factor soil basic properties soil solute transport
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Selective logging enhances ecosystem multifunctionality via increase of functional diversity in a Pinus yunnanensis forest in Southwest China 被引量:3
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作者 Xiaobo Huang Shuaifeng Li Jianrong Su 《Forest Ecosystems》 SCIE CSCD 2020年第4期733-745,共13页
Background:The impacts of selective logging on ecosystem multifunctionality(EMF)remain largely unexplored.In this study,we analyzed the response of nine variables related to four ecosystem functions(i.e.nutrient cycli... Background:The impacts of selective logging on ecosystem multifunctionality(EMF)remain largely unexplored.In this study,we analyzed the response of nine variables related to four ecosystem functions(i.e.nutrient cycling,soil carbon stocks,decomposition,and wood production)to five selective logging intensities in a Pinus yunnanensisdominated forest.We included a control group with no harvest to evaluate the potential shifts in EMF of the P.yunnanensis forests.We also assessed the relationship between above-and belowground biodiversity and EMF under these different selective logging intensities.Additionally,we evaluated the effects of biotic and abiotic factors on EMF using a structural equation modeling(SEM)approach.Results:Individual ecosystem functions(EFs)all had a significant positive correlation with selective logging intensity.Different EFs showed different patterns with the increase of selective logging intensity.We found that EMF tended to increase with logging intensity,and that EMF significantly improved when the stand was harvested at least twice.Both functional diversity and soil moisture had a significant positive correlation with EMF,but soil fungal operational taxonomic units(OTUs)had a significant negative correlation with EMF.Based on SEM,we found that selective logging improved EMF mainly by increasing functional diversity.Conclusion:Our study demonstrates that selective logging is a good management technique from an EMF perspective,and thus provide us with potential guidelines to improve forest management in P.yunnanensis forests in this region.The functional diversity is maximized through reasonable selective logging measures,so as to enhance EMF. 展开更多
关键词 BIODIVERSITY Ecosystem multifunctionality functional traits Pinus yunnanensis soil enzymatic activity structural equation modeling
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Plant-available water capacity of soils at a regional scale:Analysis of fixed and dynamic field capacities
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作者 Vinod PHOGAT Paul RPETRIE +1 位作者 Casandra COLLINS Marcos BONADA 《Pedosphere》 SCIE CAS CSCD 2024年第3期590-605,共16页
Estimation of the plant-available water capacity(PAWC)of soils at a regional scale helps in adopting better land use planning,developing suitable irrigation schedules for crops,and optimizing the use of scarce water r... Estimation of the plant-available water capacity(PAWC)of soils at a regional scale helps in adopting better land use planning,developing suitable irrigation schedules for crops,and optimizing the use of scarce water resources.In the current study,72 soil profiles were sampled from the Barossa region of South Australia to estimate pedo-transfer functions deduced from easily estimated soil properties.These functions were then used to estimate the fixed(10 and 33 kPa)and dynamic pressure head(h_(fc))water contents at field capacity(FC)for minimum drainage flux(0.01 and 0.001 cm d^(-1)),which serves as the upper boundary for plant-available water in soils.The estimated residual water content was corrected for subsoil constraints,especially the exchangeable sodium percentage(ESP).The results showed that the mean values of h_(fc)in sand-dominated light and medium textured soils(i.e.,sand,loamy sand,sandy loam,and loam)varied in a narrow range(15.8-18.2 kPa),whereas those in the clay-dominated heavy textured soils(i.e.,clay loam)showed a wide range(11.3-49.3 kPa).There were large differences in PAWC for dynamic FC(PAWC_(fc))and fixed FC at 10 kPa(PAWC10),33 kPa(PAWC33),and a mix of 10and 33 kPa(PAWC_(10,33))pressure heads depending on soil texture.Normally,the difference between PAWC at 10 kPa and h_(fc)(ΔPAWC_(10))was positive,whereas that between 33 kPa and h_(fc)(ΔPAWC_(33))was negative across all sites.Nevertheless,the estimation of PAWC assuming a fixed FC at 10 and 33 kPa pressures(i.e.,PAWC_(10,33))for sandy,clay,and silty soils reduced the difference between fixed and dynamic pressure PAWCs to<10%across the region.The estimation of PAWC was improved by incorporating the impact of subsoil constraints,such as high ESP,which was more pronounced for clay and silty soils.These findings demonstrate the inherent inconsistencies between static pressure and flux-based dynamic FC estimations in soils.Soil heterogeneity,intra-texture variability,subsoil constraints,and swell-shrink clays can have great impacts on the water retention capacity in response to dynamic and fixed pressure FC values. 展开更多
关键词 crop lower limit drained upper limit pedo-transfer function soil hydraulic parameter soil texture
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Impacts of methamidophos,copper,and their combinations on bacterial community structure and function in black soil
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作者 ZHANG Huiwen ZHOU Qixing +1 位作者 ZHANG Qianru ZHANG Chenggang 《Science China(Life Sciences)》 SCIE CAS 2005年第z1期14-25,共12页
The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rD... The potential ecotoxicologial risks of methamidophos,copper,and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach,and functional characteristics at community levels by using BIOLOG^(GN) system analysis method as well as two conventional methods(DHA and SIR).All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNA V_(3) high variable region universal primer)indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos,copper,especially some of their combinations had worse effects than their corresponding single factors.In addition,the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community.The effects of the single factors with lower concentrations on the communiy structure were weaker than those with higher concentrations.Moreover,the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors.The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOG^(GN)system were two relatively sensitive directors corresponding to the stress presented in this study.Between methamodophos and copper,there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities.The DHA of soil under the combined stresses was lower than that of the control and that under the single factors,and the BIOLOG^(GN) substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors.From all of above,the methamidophos,copper,especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure,richness,and the functional characteristics of microbial community. 展开更多
关键词 and their combinations on bacterial community structure and function in black soil Impacts of methamidophos COPPER
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Effect of Compaction on Soil Hydraulic Parameters of Vegetative Landfill Covers
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作者 Ruediger Anlauf Peter Rehrmann 《Geomaterials》 2012年第2期29-36,共8页
Parameters of water retention and air capacity are important factors for the evaluation of soil material that will be used for vegetative covers or evapotranspiration (ET) covers of landfills. These values are often m... Parameters of water retention and air capacity are important factors for the evaluation of soil material that will be used for vegetative covers or evapotranspiration (ET) covers of landfills. These values are often measured in the laboratory (usually on disturbed samples), but are also estimated from texture, organic matter content and dry bulk density. The standard basis for the estimation in Germany is the German Soil Classification Handbook (KA5). This estimation implicitly assumes that the data in the KA5 compiled from naturally developed soils are also valid for artificially compacted materials. In the present study, 25 materials were evaluated in the laboratory for the available water capacity, air capacity and permanent wilting point at 85%, 90% and 95% of Proctor density. The data were compared with parameter estimations from the KA5 and the program ROSETTA. Both estimation methods show significant deviations from the measured values;specifically, the change in the available water capacity in compressed samples is not estimated correctly. A possible explanation is a change in pore structure at different compaction levels of build in soil material in comparison with naturally developed soils of different bulk densities. 展开更多
关键词 WATER BALANCE Available WATER Capacity Air Capacity pedo-transfer function PORE structure
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黄河三角洲横向水文连通动态过程及其土壤影响因子 被引量:1
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作者 李镇 王婧雯 +4 位作者 李瑶 王以劢 史盛涵 刘佳凯 张明祥 《湿地科学》 北大核心 2025年第5期888-896,共9页
横向水文连通动态过程是维持湿地生态系统功能稳定性的关键因素,对湿地保护与生态修复具有重要意义。本研究以黄河三角洲滨海湿地为研究对象,通过对黄河三角洲滨海湿地的实地监测,系统分析了地上与地下水位的变化及土壤结构特性,并利用... 横向水文连通动态过程是维持湿地生态系统功能稳定性的关键因素,对湿地保护与生态修复具有重要意义。本研究以黄河三角洲滨海湿地为研究对象,通过对黄河三角洲滨海湿地的实地监测,系统分析了地上与地下水位的变化及土壤结构特性,并利用连续水位数据揭示了横向水文连通的时空变异规律及其驱动机制;通过对土壤结构性质进行采样与分析,结合横向水文连通动态过程,阐明横向水文连通在传播过程中受到的土壤影响因子的作用机理。研究结果表明,地上和地下横向水文连通指数随着距潮沟距离的增加呈显著下降趋势,地下水文连通性在强度和时间持续性上均优于地上水文连通性;水文连通过程对潮汐运动的响应强度随着海岸或潮沟距离的增加而逐渐衰减,不同潮沟间表现出相似的规律;土壤结构对水文连通的影响具有显著的空间分异性,地上土壤结构特征主要受控于横向水文连通时间的变化,而地下土壤结构特性主要受横向水文连通强度空间异质性影响,其中非毛管孔隙度是影响连通强度的关键因子;土壤容重、毛管孔隙度等指标通过改变水分渗透与储存能力,进一步调节水文连通的时空动态。 展开更多
关键词 黄河三角洲 水文连通 功能连通 土壤结构
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石油污染场地土壤-地下水系统介质场中微生物群落结构垂向分布和功能差异
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作者 丁妍 周爱国 +2 位作者 李小倩 何宁洁 邢新丽 《地球科学》 北大核心 2025年第5期2011-2022,共12页
重点行业场地土壤-地下水有机污染是水土环境治理修复亟待解决的重要问题,微生物群落在土壤-地下水系统中的分布对有机污染物迁移转化与生物降解具有重要作用.选取西北黄土高原某石化场地典型垂向剖面,基于16S rRNA基因高通量测序技术,... 重点行业场地土壤-地下水有机污染是水土环境治理修复亟待解决的重要问题,微生物群落在土壤-地下水系统中的分布对有机污染物迁移转化与生物降解具有重要作用.选取西北黄土高原某石化场地典型垂向剖面,基于16S rRNA基因高通量测序技术,精细刻画土壤-包气带-潜水含水层-弱透水层连续非均质介质场中微生物群落结构、多样性的垂向分布特征及其代谢功能差异,揭示岩性、深度因素对微生物群落结构和功能垂向分布的影响.研究表明,土壤-地下水系统介质场中微生物群落结构与多样性的垂向分布存在显著差异,并表现出不同的代谢功能和石油污染物降解模式.包气带层中的丙酸杆菌目、潜水含水层中的β-变形菌目既是优势菌种,又是组间的标志差异菌种,贡献了相关主要差异代谢功能.深度和岩性分别影响了不同的代谢功能,包气带、含水层及其下伏弱透水层中微生物以协同作用形式分别围绕芳香族化合物降解、暗氢氧化功能实现石油污染物的降解. 展开更多
关键词 介质场 微生物 群落结构 代谢功能 地下水 土壤-地下水系统 污染场地 黄土高原 水文地质.
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有机物料还田对稻田土壤细菌群落和水稻产量的影响 被引量:4
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作者 卞清 孙波 +2 位作者 李大明 谢祖彬 王晓玥 《环境科学》 北大核心 2025年第1期543-550,共8页
为探明长期有机物料还田下稻田土壤微生物群落结构及功能对水稻产量的影响,依托于40 a红壤性水稻土有机肥定位试验,选择不施肥(CK)、施化肥(NPK)、施化肥+紫云英(NG)、施化肥+紫云英+猪粪(NGM)和施化肥+紫云英+稻草(NGS)这5个处理,开展... 为探明长期有机物料还田下稻田土壤微生物群落结构及功能对水稻产量的影响,依托于40 a红壤性水稻土有机肥定位试验,选择不施肥(CK)、施化肥(NPK)、施化肥+紫云英(NG)、施化肥+紫云英+猪粪(NGM)和施化肥+紫云英+稻草(NGS)这5个处理,开展相关研究.结果表明,有机物料还田显著提高了土壤SOC、TN和TP等养分含量和水稻产量,其中NGM处理影响最大.并且有机物料还田显著改变了微生物群落组成,主要表现为:变形菌门,特别是α-变形菌,以及放线菌门的相对丰度增加,而硝化螺旋菌门的相对丰度减少. PICRUSt2功能预测分析结果表明,有机物料还田增加了编码淀粉(amyA)、纤维素(bglX、bglB、endoglucanase)和半纤维素(xylA、xynA)等与易分解碳降解相关的功能基因的丰度,同时也增加了编码脲酶(ureA、ureB和ureC)、谷氨酸脱氢酶(gdhA)和谷氨酰胺酶(aspQ)等与有机氮矿化相关的功能基因的丰度.基于随机森林模型分析,影响水稻产量的理化和生物因子包括:TP、SOC、pH、TN、DON、细菌群落组成以及有机碳降解功能基因和有机氮矿化功能基因的相对丰度. PLS-PM结果进一步表明,有机物料还田下,SOC和TP含量的增加通过改变细菌群落结构,特别是增加了α-变形菌和放线菌等富营养型细菌的相对丰度,增加了土壤有机碳、氮分解相关功能基因的相对丰度,促进养分循环,从而提高水稻产量. 展开更多
关键词 红壤性水稻土 有机物料还田 细菌群落结构 功能基因 水稻产量
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不同生境荒漠植物土壤细菌群落结构及功能特征 被引量:1
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作者 杨现瑶 吴雪 +4 位作者 田翠萍 杨倩倩 汪显涛 张旋 郑洲 《环境科学》 北大核心 2025年第9期5999-6009,共11页
为探究不同生境下荒漠植物-微生物共生系统的3个要素(植物、优势细菌群落和土壤环境)之间的作用关系,以塔里木盆地北缘荒漠和绿洲过渡带典型植物多枝柽柳、骆驼刺和花花柴为研究对象,采用高通量测序技术探究两种生境下其根区土壤细菌群... 为探究不同生境下荒漠植物-微生物共生系统的3个要素(植物、优势细菌群落和土壤环境)之间的作用关系,以塔里木盆地北缘荒漠和绿洲过渡带典型植物多枝柽柳、骆驼刺和花花柴为研究对象,采用高通量测序技术探究两种生境下其根区土壤细菌群落结构组装、多样性和功能差异.结果表明:①土壤细菌群落受到环境和遗传因素的共同影响.②变形菌门、拟杆菌门和放线菌门为过渡带生境下3种植物的优势细菌门;荒漠生境则是拟杆菌门、变形菌门和厚壁菌门为优势菌门.③土壤含水量和电导率与土壤细菌群落结构显著相关(P<0.05),荒漠-绿洲过渡带土壤含水量显著高于荒漠生境,掩盖了其他环境因子对微生物的影响,成为影响土壤微生物群落结构和功能的关键因子.④通过PICRUSt2功能预测发现,3种典型荒漠植物土壤细菌群落中具有新陈代谢功能的菌种具有明显优势,不同植物在细菌功能富集表现上具有差异.研究结果增进了典型荒漠植物和生境差异对土壤微生物多样性及基因功能影响的认识,可为后续荒漠生态治理和维持生态系统稳定提供土壤微环境参考. 展开更多
关键词 荒漠-绿洲过渡带 土壤理化性质 土壤微生物 群落结构 功能预测
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不同银杉种群根际土壤微生物群落结构与功能差异 被引量:1
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作者 王玉凤 李双林 +3 位作者 刘宏 邓涛 叶家桐 胡兴华 《林业科学研究》 北大核心 2025年第1期73-85,共13页
[目的]通过探究不同银杉种群根际土壤微生物群落的结构及功能差异,旨在探讨根际土壤微生物群落对银杉生长发育的影响。[方法]以广西桂林花坪国家级自然保护区的红滩、谢塘湾和野猪塘3个银杉种群为研究对象,提取其根际土壤微生物总DNA,... [目的]通过探究不同银杉种群根际土壤微生物群落的结构及功能差异,旨在探讨根际土壤微生物群落对银杉生长发育的影响。[方法]以广西桂林花坪国家级自然保护区的红滩、谢塘湾和野猪塘3个银杉种群为研究对象,提取其根际土壤微生物总DNA,进行宏基因组测序,比较分析银杉人工种群(红滩)与自然种群(谢塘湾、野猪塘)根际土壤微生物群落结构与功能之间的差异,并探索造成该差异的主导土壤环境因子。[结果](1)银杉人工种群和自然种群根际土壤微生物群落结构存在显著差异,主要表现在相对丰度占比最大的3个菌门,即变形菌门、放线菌门和酸杆菌门;(2)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落结构差异的主导因子;(3)银杉人工种群和自然种群根际土壤微生物群落功能存在显著差异,主要表现在甘氨酸的生物合成和代谢(Glycan biosynthesis and metabolism)、寄生虫传染病(Infectious disease:parasitic)、其他氨基酸代谢(Metabolism of other amino acids)等方面;(4)土壤p H值和TK含量是造成银杉人工种群和自然种群根际土壤微生物群落功能差异的主导因子。影响银杉根际土壤微生物群落结构与功能的土壤环境因子具有一致性。[结论]银杉的根际土壤微生物群落结构与功能在自然种群中表现得比人工种群的更复杂,但两类种群中影响其结构与功能的主导土壤环境因子是一致的,在实施银杉种苗野外回归种植时,应充分考虑根际土壤微生物群落对银杉种苗生长的影响。 展开更多
关键词 宏基因组 根际土壤微生物 群落结构 功能差异 银杉
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高寒黑土区不同轮作模式对马铃薯根际土壤细菌群落结构与功能的影响 被引量:1
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作者 王籽懿 韩伟 +2 位作者 金鸿飚 黄修梅 李明 《江苏农业学报》 北大核心 2025年第2期286-295,共10页
为明确不同轮作模式对马铃薯根际土壤细菌群落的影响,本研究以马铃薯-小麦-甜菜-马铃薯(A处理)、马铃薯-水飞蓟-小麦-马铃薯(B处理)、小麦-甜菜-小麦-马铃薯(C处理),油菜-小麦-甜菜-马铃薯(D处理),小麦-油菜-大麦-马铃薯(E处理)和油菜-... 为明确不同轮作模式对马铃薯根际土壤细菌群落的影响,本研究以马铃薯-小麦-甜菜-马铃薯(A处理)、马铃薯-水飞蓟-小麦-马铃薯(B处理)、小麦-甜菜-小麦-马铃薯(C处理),油菜-小麦-甜菜-马铃薯(D处理),小麦-油菜-大麦-马铃薯(E处理)和油菜-小麦-马铃薯-马铃薯(CK,对照)6种种植模式为研究对象,采用细菌16S区的高通量测序技术,对比分析当地6种种植模式对马铃薯根际土壤细菌群落结构和功能的影响。结果表明,6种轮作模式的土壤样本共获得36420个扩增子序列变异(ASV),划分为45门、119纲、298目、451科、790属和357种。不同轮作模式马铃薯根际土壤主要优势菌群同为变形菌门、酸杆菌门、拟杆菌门、芽单胞菌门、放线菌门、浮霉菌门、疣微菌门、绿弯菌门、黏球菌门、髌骨菌门,但主要优势菌群的相对丰度在不同轮作模式中存在差异。与CK相比,轮作明显提高了细菌中酸杆菌门和芽单胞菌门细菌的相对丰度,而放线菌门细菌相对丰度明显降低,溶杆菌属细菌以及与碳、氮相关的功能菌相对丰度升高。组间群落差异分析(LEfSe)结果表明,在细菌群落中共鉴定出38个差异物种。通过基因功能预测发现,一级功能中新陈代谢的相对丰度最大(62.04%~62.46%),相对丰度大于1.0%的二级功能代谢通路有19类,其中碳水化合物代谢的相对丰度最高(12.93%~13.29%)。综上所述,轮作改变了土壤细菌群落结构,提高了土壤细菌中的优势功能菌,其中以小麦-甜菜-小麦-马铃薯轮作模式效果最好,更适宜高寒黑土区马铃薯产业的可持续发展。 展开更多
关键词 高寒黑土区 轮作模式 马铃薯 根际土壤 细菌群落结构 细菌群落功能
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