Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of al...Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network.Alginate nanobeads developed by calcium cross-linking yielded a size of 200e500 nm.The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus.Characterization of the in situ injectable hydrogel was done by SEM,FTIR and Rheometer.The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering.Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment,proliferation and infiltration within the hydrogel similar to fibrin gel.The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.展开更多
To further improve the biocompatibility of polyurethanes,a new lysine 2-(2-aminoethoxy)ethanol phosphatidylcholine(LAPC)is synthesized to use as chain extender for preparing a series of phosphatidylcholine polyurethan...To further improve the biocompatibility of polyurethanes,a new lysine 2-(2-aminoethoxy)ethanol phosphatidylcholine(LAPC)is synthesized to use as chain extender for preparing a series of phosphatidylcholine polyurethanes(PCPUs).Poly(e-caprolactone)(PCL)and poly(ethylene glycol)(PEG)are used as soft segments,and L-lysine ethyl ester diisocyanate(LDI),LAPC and L-lysine are used as hard segments.The obtained PCPUs exhibit appropriate mechanical properties with break elongation of 1000–1700%,tensile strength of 7–22 MPa,and relatively high elastic modulus of 11–18 MPa,which could admirably satisfy the requirement for soft tissue engineering scaffolds.The phosphatidylcholine structures can increase the hydrophilicity of PCPU surfaces,which effectively reduce protein adsorption and platelet adhesion while promoting the cell proliferation.In addition,the LAPC chain extender,PCPU films and ultimate degradation products of PCPUs are proved to be nontoxic in cytotoxicity test.More interestingly,the cytokine release test of macrophages manifests that both LAPC and PCPU degradation products could effectively improve the proliferation of macrophages and induce them into a wound-healing phenotype.Thus,the obtained PCPUs have greatly potential applications of soft tissue engineering scaffolds for tissue repair and wound healing.展开更多
The current study investigated a triad,which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol(PVA)-based matrix with exclusive ascorbic acid signalling.T...The current study investigated a triad,which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol(PVA)-based matrix with exclusive ascorbic acid signalling.Though,the bio-mechanical properties of the gelatin–PVA blended scaffolds in wet condition are equivalent to the ECM of soft tissues in general,in this study,the triad was tested as a model for neural tissue engineering.Apart from being cytocompatible and biocompatible,the porosity of the scaffold has been designed in such a manner that it facilitates the cell signalling and enables the exchange of nutrients and gases.The highly proliferative stem cells from Passage 2 were characterized using both,mesenchymal and embryonic stem cell markers.As an initial exploration the mesenchymal stem cells at Passage 4 were exposed to ascorbic acid and basic fibroblast growth factor signalling for neuronal differentiation in 2D environment independently.The MSCs successfully differentiated and acquired neuron specific markers related to cytoskeleton and synapses.Subsequently,three phases of experiments have been conducted on the 3D gelatin/PVA matrix to prove their efficacy,the growth of stem cells,growth of differentiated neurons and the in situ growth and differentiation of MSCs.The scaffold was conducive and directed MSCs to neuronal lineage under specific signalling.Overall,this organotypic model triad could open a new avenue in the field of soft tissue engineering as a simple and effective tissue construct.展开更多
Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth...Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.展开更多
基金One of the authors R.Jayakumar is grateful to the Department of Biotechnology(DBT),India,for providing fund under the scheme of Nanoscience and Nanotechnology Program(Ref.No.BT/PR 13585/NNT/28/474/2010)One of the author S.Deepthi is thankful to the Council of Scientific and Industrial Research for supporting financially under the CSIR-SRF award no:9/963(0034)2K13-EMR-I.
文摘Hydrogels are a class of materials that has the property of injectability and in situ gel formation.This property of hydrogels is manipulated in this study to develop a biomimetic bioresorbable injectable system of alginate nanobeads interspersed in fibrin network.Alginate nanobeads developed by calcium cross-linking yielded a size of 200e500 nm.The alginate nanobeads fibrin hydrogel was formed using dual syringe apparatus.Characterization of the in situ injectable hydrogel was done by SEM,FTIR and Rheometer.The developed hydrogel showed mechanical strength of 19 kPa which provides the suitable compliance for soft tissue engineering.Cytocompatibility studies using human umbilical cord blood derived mesenchymal stem cells showed good attachment,proliferation and infiltration within the hydrogel similar to fibrin gel.The developed in situ forming hydrogel could be a suitable delivery carrier of stem cells for soft tissue regeneration.
基金This work was supported by the National Natural Science Foundation of China(51273124,51333003 and 51573114)the National Science Fund for Distinguished Young Scholars of China(51425305).
文摘To further improve the biocompatibility of polyurethanes,a new lysine 2-(2-aminoethoxy)ethanol phosphatidylcholine(LAPC)is synthesized to use as chain extender for preparing a series of phosphatidylcholine polyurethanes(PCPUs).Poly(e-caprolactone)(PCL)and poly(ethylene glycol)(PEG)are used as soft segments,and L-lysine ethyl ester diisocyanate(LDI),LAPC and L-lysine are used as hard segments.The obtained PCPUs exhibit appropriate mechanical properties with break elongation of 1000–1700%,tensile strength of 7–22 MPa,and relatively high elastic modulus of 11–18 MPa,which could admirably satisfy the requirement for soft tissue engineering scaffolds.The phosphatidylcholine structures can increase the hydrophilicity of PCPU surfaces,which effectively reduce protein adsorption and platelet adhesion while promoting the cell proliferation.In addition,the LAPC chain extender,PCPU films and ultimate degradation products of PCPUs are proved to be nontoxic in cytotoxicity test.More interestingly,the cytokine release test of macrophages manifests that both LAPC and PCPU degradation products could effectively improve the proliferation of macrophages and induce them into a wound-healing phenotype.Thus,the obtained PCPUs have greatly potential applications of soft tissue engineering scaffolds for tissue repair and wound healing.
基金National Foundation for Liver Research(NFLR)for financially supporting the cell-based work in this project.CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011,Iniciativa Ingenio 2010,Consolider Program.CIBER actions are financed by the Instituto de Salud CarlosⅢwith assistance from the European Regional Development Fundthe University Grants Commission,India for the award of the Basic Scientific Research(UGC-BSR)Faculty Fellowship[No.F.4-5(11)2019(BSR)].
文摘The current study investigated a triad,which comprises of adipose tissue derived stem cells isolated from infrapatellar fat pad and gelatin/polyvinyl alcohol(PVA)-based matrix with exclusive ascorbic acid signalling.Though,the bio-mechanical properties of the gelatin–PVA blended scaffolds in wet condition are equivalent to the ECM of soft tissues in general,in this study,the triad was tested as a model for neural tissue engineering.Apart from being cytocompatible and biocompatible,the porosity of the scaffold has been designed in such a manner that it facilitates the cell signalling and enables the exchange of nutrients and gases.The highly proliferative stem cells from Passage 2 were characterized using both,mesenchymal and embryonic stem cell markers.As an initial exploration the mesenchymal stem cells at Passage 4 were exposed to ascorbic acid and basic fibroblast growth factor signalling for neuronal differentiation in 2D environment independently.The MSCs successfully differentiated and acquired neuron specific markers related to cytoskeleton and synapses.Subsequently,three phases of experiments have been conducted on the 3D gelatin/PVA matrix to prove their efficacy,the growth of stem cells,growth of differentiated neurons and the in situ growth and differentiation of MSCs.The scaffold was conducive and directed MSCs to neuronal lineage under specific signalling.Overall,this organotypic model triad could open a new avenue in the field of soft tissue engineering as a simple and effective tissue construct.
基金supported by the National Key Research and Development Program of China(2017YFA0701302,PKUSS20200113).
文摘Oral soft tissue defects remain difficult to treat owing to the limited efficacy of available treatment materials.Although the injectable platelet-rich fibrin(i-PRF)is a safe,autologous source of high levels of growth factors that is often employed to promote the regeneration of oral soft tissue,its effectiveness is restrained by difficulties in intraoperative shaping together with the burst-like release of growth factors.We herein sought to develop a bioactive bioink composed of i-PRF,alginate and gelatin capable of promoting the regeneration of the oral soft tissue.This bioink was successfully applied in 3D bioprinting and exhibited its ability to be shaped to individual patient needs.Importantly,we were also able to significantly prolong the duration of multiple growth factors release as compared to that observed for i-PRF.The growth factor bioavailability was further confirmed by the enhanced proliferation and viability of printed gingival fibroblasts.When deployed in vivo in nude mice,this bioink was further confirmed to be biocompatible and to drive enhanced angiogenic activity.Together,these data thus confirmthe successful production of an i-PRF-containing bioink,which is suitable for the individualized promotion of the regeneration of oral soft tissue.
