It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated w...It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated with single-chain Fv antibody against γ-seminoprotein.The resultant nanoprobes showed highly selective targeting,fluorescent imaging,and magnetic resonance imaging.The cytotoxicity effects were investigated on the prostate cancer cells and solid tumors under in vitro alternating magnetic field irradiation.It was found that the as-prepared nanoprobes did not show signs of toxicity within the used maximal dosage.It was also observed that the tumors implanted in nude mice were significantly reduced in size and disappeared gradually due to thermal treatment.The lifespan of post-therapeutic mice loaded with prostate cancer was considerable prolonged.High-performance single-chain Fv antibody againstγ-seminoprotein-conjugated fluorescent magnetic nanoparticles may have great potential in applications such early detection and localized thermal therapy of prostate cancer.展开更多
基金supported by China National 973 Project(2010CB933901)National 863 Hi-tech Project(2007AA022004)+1 种基金National Natural Scientific Fund(Nos.20771075and 30672147)the science and technology commission Fund of shanghai government(No.072112006 and No.0752nm024).
文摘It has been a challenging task to develop nontoxic nanoprobes for targeted-imaging and selective therapy of prostate cancer.Herein,fluorescent superparamagnetic nanoparticles with a diameter of 50 nm were conjugated with single-chain Fv antibody against γ-seminoprotein.The resultant nanoprobes showed highly selective targeting,fluorescent imaging,and magnetic resonance imaging.The cytotoxicity effects were investigated on the prostate cancer cells and solid tumors under in vitro alternating magnetic field irradiation.It was found that the as-prepared nanoprobes did not show signs of toxicity within the used maximal dosage.It was also observed that the tumors implanted in nude mice were significantly reduced in size and disappeared gradually due to thermal treatment.The lifespan of post-therapeutic mice loaded with prostate cancer was considerable prolonged.High-performance single-chain Fv antibody againstγ-seminoprotein-conjugated fluorescent magnetic nanoparticles may have great potential in applications such early detection and localized thermal therapy of prostate cancer.
