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Flow Pattern and Pressure Fluctuation of Severe Slugging in Pipeline-riser System 被引量:14
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作者 罗小明 何利民 马华伟 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2011年第1期26-32,共7页
During the exploitation of offshore oil and gas,it is easy to form severe slugging which can cause great harm in the riser connecting wellheads and offshore platform preprocessing system.The flow pattern and pressure ... During the exploitation of offshore oil and gas,it is easy to form severe slugging which can cause great harm in the riser connecting wellheads and offshore platform preprocessing system.The flow pattern and pressure fluctuation of severe slugging were studied in an experimental simulation system with inner diameter of 0.051 m.It is found that severe slugging can be divided into three severe slugging regimes:regime I at low gas and liquid flow rates with large pressure fluctuation,intermittent flow of liquid and gas in the riser,and apparent cutoff of liquid phase,regime II at high gas flow rate with non-periodic fluctuation and discontinuous liquid outflow and no gas cutoff,regime III at high liquid flow rate with degenerative pressure fluctuation in form of relatively stable bubbly or plug flow.The results indicate that severe slugging still occurs when the declination angle of pipeline is 0,and there are mainly two kinds of regimes:regime I and regime II.As the angle increases,the formation ranges of regime I and regime III increase slightly while that of regime II is not affected.With the increase of gas superficial velocity and liquid superficial velocity,the pressure fluctuation at the bottom of riser increases initially and then decreases.The maximum value of pressure fluctuation occurs at the transition boundary of regimes I and II. 展开更多
关键词 multiphase flow severe slugging pipeline-riser system flow regime pressure fluctuation
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Influence of separator control on the characteristics of severe slugging flow 被引量:5
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作者 Luo Xiaoming He Limin +1 位作者 Liu Xinye Lü Yuling 《Petroleum Science》 SCIE CAS CSCD 2014年第2期300-307,共8页
Due to the special structure of offshore multiphase pipes, it is easy for severe slugging to occur in the riser at low gas-liquid velocity. Violent pressure fluctuations and dramatic changes of flow rate are the main ... Due to the special structure of offshore multiphase pipes, it is easy for severe slugging to occur in the riser at low gas-liquid velocity. Violent pressure fluctuations and dramatic changes of flow rate are the main characteristics of severe slugging, leading to the risk of serious damage. In this paper, the separator control is adopted to accurately control the separator liquid level and pressure under severe slugging flow conditions. This indicates that the separator liquid level control alone does not have a significant impact on the upstream flow, but it is beneficial for normal operation and pressure control of the separator. As the separator pressure increases, the peak pressure in the riser apparently diminishes, and the amplitude of pressure fluctuation gradually decreases, which means that severe slugging is inhibited. During the slug blowing out, the gas/liquid slipping in the riser intensifies. The long gas plug quickly flows through the riser, and then tends to morph into short and slowly flowing gas bubbles. The elimination effect of the pressure control strategy on severe slugging is related to the relative rate of the superficial gas/liquid flow. 展开更多
关键词 Multiphase flow severe slugging separator control RISER
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Experimental and Computational Investigations on Severe Slugging in A Catenary Riser 被引量:4
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作者 DUAN Jin-long CHEN Ke +1 位作者 YOU Yun-xiang GAO Song 《China Ocean Engineering》 SCIE EI CSCD 2017年第6期653-664,共12页
Severe slugging can occur in a pipeline-riser system at relatively low liquid and gas flow rates during gas-oil transportation, possibly causing unexpected damage to the production facilities. Experiments with air and... Severe slugging can occur in a pipeline-riser system at relatively low liquid and gas flow rates during gas-oil transportation, possibly causing unexpected damage to the production facilities. Experiments with air and water are conducted in a horizontal and downward inclined pipeline followed by a catenary riser in order to investigate the mechanism and characteristics of severe slugging. A theoretical model is introduced to compare with the experiments. The results show that the formation mechanism of severe slugging in a catenary riser is different from that in a vertical riser due to the riser geometry and five flow patterns are obtained and analyzed. A gas-liquid mixture slug stage is observed at the beginning of one cycle of severe slugging, which is seldom noticed in previous studies. Based on both experiments and computations, the time period and variation of pressure amplitude of severe slugging are found closely related to the superficial gas velocity, implying that the gas velocity significantly influences the flow patterns in our experiments. Moreover, good agreements between the experimental data and the numerical results are shown in the stability curve and flow regime map, which can be a possible reference for design in an offshore oil-production system. 展开更多
关键词 severe slugging flow patterns pipeline-catenary-riser system superficial gas and liquid velocities theoretical model
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Numerical Investigation on Severe Slugging in A Catenary Riser 被引量:1
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作者 QI Xiao-liang GAO Song GUO Hai-yan 《China Ocean Engineering》 SCIE EI CSCD 2019年第5期628-635,共8页
A mathematical model is presented to study the main characteristics of severe slugging in a downward inclined pipeline followed by a catenary riser. In this model, both simplified transient model and phase distributio... A mathematical model is presented to study the main characteristics of severe slugging in a downward inclined pipeline followed by a catenary riser. In this model, both simplified transient model and phase distribution model are included so that the flow characteristics of each stage for severe slugging can be accurately reproduced, especially for blowout stage. The results show that the flow features of severe slugging can be simulated by the proposed mathematical model. A good agreement between the experimental data and the numerical results is observed. The model can predict the transient fluctuation of many respects, such as the superficial gas velocity at the bottom of the riser and the average velocity at the outlet of the riser. 展开更多
关键词 SEVERE slugging pipeline-riser system phase distribution transient FLUCTUATION
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Potential variables for automatic slugging control by topside choking in pipeline-riser systems
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作者 Yaohua Du Hanxuan Wang +3 位作者 Quanhong Wu Luhan Xu Yiwei Yan Suifeng Zou 《International Journal of Fluid Engineering》 2025年第3期48-62,共15页
When using topside choking to eliminate severe slugging in offshore pipeline-riser systems,the resulting stability and effectiveness depend critically on the choice of variables in the associated control model.In this... When using topside choking to eliminate severe slugging in offshore pipeline-riser systems,the resulting stability and effectiveness depend critically on the choice of variables in the associated control model.In this study,both potential process variables and manipulated variables are investigated via manual choking experiments.Monotonicity and linearity are adopted as principles for selecting the optimal variables,and the availability of signals in an offshore field is also considered.Both amplitude-domain and time-domain variables are studied,of which five are recommended as the process variable,while the resistance factor of the choking valve is the optimal manipulated variable;other variables may act as auxiliary variables in the control model.How to determine the set value of the process variable is also discussed.Validation experiments with automatic control show that the optimized variables enable faster stabilization.This study offers a reference for the design of slugging control for offshore fields. 展开更多
关键词 severe slugging automatic slugging control pipeline riser systems topside choking associated control modelin manipulated variables manual choking experimentsmonotonicity linearity process variables
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Onset of slugging fluidization in supercritical water fluidized bed 被引量:2
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作者 Hao Wang Jikai Huang Youjun Lu 《Particuology》 SCIE EI CAS CSCD 2020年第5期47-56,共10页
The supercritical-water fluidized bed(SCWFB)is a reactor for coal and biomass gasification without pollutant emission.We carried out a series of experiments in a SCWFB,and a dual-capacitance probe measurement system w... The supercritical-water fluidized bed(SCWFB)is a reactor for coal and biomass gasification without pollutant emission.We carried out a series of experiments in a SCWFB,and a dual-capacitance probe measurement system was applied to measure the hydrodynamics of slugs,such as the slug frequency,chord length,and rising velocity.Four groups of Geldart-B particles with different mean diameters were fluidized by supercritical water with a system pressure of 20-27MPa and at 410-570℃.The minimum slugging Reynolds number increases logarithmically with Archimedes number and a predicting correlation of the minimum slugging fluidization velocity in the SCWFB is presented:Rems=32908.84 ln(Ar^0.55+260376.65)-410361.90.The relative error of the above correlation was within ±15% and the averaged relative error was 7.5%.The effect of operating conditions on the minimum slugging fluidization velocity is discussed.This research provides useful guidance for scaling-up design and for determining the optimum range of operating conditions in the SCWFB. 展开更多
关键词 Supercritical water fluidized bed slugging fluidization Dual-capacitance probe method
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Three-dimensional dynamic characterization of square-nosed slugging phenomena in a fluidized bed
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作者 Yaswanth Pottimurthy Dawei Wang +3 位作者 Cody Park Shalin Patil Andrew Tong Liang-Shih Fan 《Particuology》 SCIE EI CAS CSCD 2022年第8期35-46,共12页
Slugging represents one of the major regimes in fluidization,which occurs in small diameter beds with large bed height-to-diameter ratio or in large diameter beds with internals that resemble multiple small diameter f... Slugging represents one of the major regimes in fluidization,which occurs in small diameter beds with large bed height-to-diameter ratio or in large diameter beds with internals that resemble multiple small diameter fluidized beds.Slug types include round-nosed slug,wall slug and square nosed slug.Studies of the slugs have been mainly focused on round-nosed or wall slugs known as half slug.typically occurring in Geldart group A particle fuidization.The square-nosed slug typically occurring for Geldart group D particles appears to be regarded as simple in its structure.The Electrical Capacitance Volume Tomogra phy(ECVT)imaging of the square-nosed slugging phenomena conducted in this study reveals otherwise.That is the structure of the square nosed slug is,in fact,complex,particularly with respect to its dynamic variation in fluidization.More broadly.this study examines experimentally the hydrodynamic character-istics of the square-nosed fluidization regime.Specifically,simultaneous measurements from multiple ECVT sensors provide non-inv asive,continuous,3.dimensional imaging of the entire flow region of the slugging bed and hence enabling the dynamic characterization of the evolution of the slugs.The analysis of the 3D images reconstructed for real time gas-solid volume fraction profile of the slugging fluidized bed indicates that there are three different zones.namely.the bottom fluidization zone,the gas slug zone,and the solid slug zone,co-existing in the bed.The three zones present different hydrodynamic characteristics during the slug evolution.It is found that varying the gas velocity of the slugging bed mainly varies the maximum length of the gas slug zone,while it only has a minor effect on the lengths of the bottom fluidization zone and solid slug zone.It also has an insignificant efect on the solid volume fraction of the three zones. 展开更多
关键词 Gas-solid flow Fluidized bed Square-nosed slugging Electrical Capacitance Volume Tomography
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重楼皂苷Ⅰ对肺癌A549细胞增殖、细胞周期、迁移、侵袭及代谢物的影响
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作者 傅厚道 张瑾 +7 位作者 殷张昕 胡蔚 陈雪慧 闻路红 陈安琪 刘丽琴 胡旭 张顺 《中国中医药科技》 2025年第2期226-231,共6页
目的:研究重楼皂苷Ⅰ对A549细胞增殖、细胞周期、迁移和侵袭的抑制作用,并基于迁移相关蛋白和单细胞代谢检测探讨其作用机制。方法:采用不同浓度重楼皂苷Ⅰ处理人肺癌A549细胞,使用CCK-8检测细胞毒性及增殖抑制作用;流式细胞术检测细胞... 目的:研究重楼皂苷Ⅰ对A549细胞增殖、细胞周期、迁移和侵袭的抑制作用,并基于迁移相关蛋白和单细胞代谢检测探讨其作用机制。方法:采用不同浓度重楼皂苷Ⅰ处理人肺癌A549细胞,使用CCK-8检测细胞毒性及增殖抑制作用;流式细胞术检测细胞周期;细胞划痕实验和Tranwell侵袭实验检测细胞迁移抑制作用;Western blot检测细胞迁移和细胞粘连相关蛋白(P-p38、Slug、β-catenin、Claudin-1、N-Cadherin、MMP-2)表达;单细胞质谱检测细胞代谢物差异。结果:重楼皂苷Ⅰ可明显抑制A549细胞的增殖和迁移,显著降低P-p38,Slug,β-catenin,Claudin-1和N-Cadherin、MMP-2蛋白表达以及脂类代谢物水平。结论:重楼皂苷Ⅰ可以抑制A549细胞增殖、迁移、侵袭,降低细胞迁移和细胞粘连相关蛋白表达和细胞代谢水平。 展开更多
关键词 重楼皂苷Ⅰ 肺癌A549细胞 细胞周期 迁移 侵袭 细胞代谢物 P-P38 Slug β-catenin CLAUDIN-1 N-CADHERIN MMP-2 体外实验
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基于生物信息学探讨HOXB9调控TGF-β1Smad2Slug信号通路介导头颈鳞癌转移的机制
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作者 熊文敏 万巧玉 +4 位作者 涂子为 叶新芊 敖枫华 彭世义 姜斯聪 《中国现代药物应用》 2025年第19期177-180,共4页
目的研究同源盒基因(HOX)B9调控TGF-β1Smad2Slug信号通路介导头颈鳞癌转移的机制。方法通过TCGA数据库解读HOXB9基因在头颈鳞癌内的表达差异,并通过逆转录-实时荧光定量聚合酶链式反应(RT-qPCR)测试临床头颈鳞癌样本中HOXB9的表达水平... 目的研究同源盒基因(HOX)B9调控TGF-β1Smad2Slug信号通路介导头颈鳞癌转移的机制。方法通过TCGA数据库解读HOXB9基因在头颈鳞癌内的表达差异,并通过逆转录-实时荧光定量聚合酶链式反应(RT-qPCR)测试临床头颈鳞癌样本中HOXB9的表达水平,通过Kaplan-Meier模式解读HOXB9与头颈鳞癌患者临床预后的关系,通过调整HOXB9在头颈鳞癌细胞株TGF-β1Smad2Slug中的表达,使用CCK-8模式与克隆来进行试验,并测试头颈鳞癌细胞增殖与克隆功能,流式细胞术双染色模式测试癌症细胞凋亡率,Western blot(WB)测试凋亡蛋白的表达。结果生物信息学解读TCGA数据库发现HOX在头颈鳞癌内高表达(P<0.001),HOX在癌组织中明显高表达且大于肿瘤旁组织(P<0.001)。预后分析说明,HOX高表达患者总生存期(OS)显著缩短,风险提升了0.42倍(P=0.01),HOXB9高低表达组在癌症的级别上出现差异(P<0.001),在整体存活期方面出现差异(P<0.01)。RT-qPCR证实转染shHOXC6质粒基因相比于转染shNC质粒基因,HOXC6 mRNA表达明显下降(P<0.01)。WB显示转染shHOXC6质粒的基因相比于转染shNC质粒的基因,HOX蛋白的表达明显下降(P<0.01)。靶向circE7的siRNA转染后,肿瘤细胞人白细胞抗原I(HLA-I)表达恢复,并且程序性死亡受体1(PD-1)抑制剂疗效提高2.3倍。结论HOX在头颈鳞癌中高表达,并带动头颈鳞癌的繁殖,抑制头颈鳞癌的凋亡,进而致使预后较差。 展开更多
关键词 生物信息学 同源盒基因B9调控 TGF-β1Smad2Slug信号通路 介导机制 头颈鳞癌转移机制
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Investigation on Fatigue Damage of Offshore Risers Due to Slug-Induced Vibrations Based on Arbitrary Lagrangian-Eulerian(ALE)-Absolute Nodal Coordinate Formulation(ANCF) 被引量:1
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作者 LIU De-peng ZHANG Yu AI Shang-mao 《China Ocean Engineering》 2025年第5期941-955,共15页
This study examines the slug-induced vibration(SIV)response and fatigue behavior of offshore risers subjected to internal slug flow.A structural model incorporating internal slug flow dynamics is developed using the A... This study examines the slug-induced vibration(SIV)response and fatigue behavior of offshore risers subjected to internal slug flow.A structural model incorporating internal slug flow dynamics is developed using the Absolute Nodal Coordinate Formulation(ANCF)and a spatial-temporal density variation equation to analyze how slug flow parameters affect the SIV response of risers.Structural displacement,stress,and fatigue responses are systematically evaluated to characterize the structural behavior under SIV conditions.Longer slugs induce more pronounced traveling wave characteristics,while shorter slugs facilitate a mixed traveling-standing wave mode.Moreover,higher slug frequencies lead to increased fatigue accumulation,especially over an extended touchdown zone,thereby compromising the structural integrity of the riser.The findings yield valuable insights into the dynamic interactions between slug flow and riser response.This research advances the understanding of SIV mechanisms and provides a theoretical foundation for fatigue assessment and structural optimization,contributing to the safe and efficient design of offshore risers in deepwater environments. 展开更多
关键词 slug flow offshore riser fatigue damage dynamic analysis
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Experimental Investigation of the Slug-Induced Vibration of a Flexible Catenary Riser at Variable Gas-Liquid Ratios and Mixture Velocities 被引量:1
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作者 GAO Yue ZHU Hong-jun +2 位作者 XU Bing WANG Min-hao ZHOU Mai 《China Ocean Engineering》 2025年第5期839-854,共16页
An experimental analysis of the slug-induced vibration(SIV)of a flexible catenary riser with an aspect ratio of 130 is reported in this work.The vibration responses and internal slug flow details at different gas-liqu... An experimental analysis of the slug-induced vibration(SIV)of a flexible catenary riser with an aspect ratio of 130 is reported in this work.The vibration responses and internal slug flow details at different gas-liquid ratios(Q_(g)/Q_(l))and mixture velocities(vm)are non-intrusively and simultaneously captured by high-speed cameras.Both the in-plane and out-of-plane responses of the catenary riser are excited in all considered cases.The slug flow characteristics,including translational velocities,slug lengths,recurrence frequencies,and pressure variations,are analyzed and dis-cussed,as are the dynamic responses of the riser in terms of the amplitudes,space-varying frequency,and modal weight.The dominant modal response based on the root-mean-square amplitude profiles does not match that based on the dominant frequency.Three mode switching types are identified based on the RMS amplitude profiles and the dominant frequency.When vm is small,no mode switching(NMS)is observed in either the in-plane or out-of-plane responses.For mode switching I(MS I),the switching between the first and second modes in the in-plane response is induced by slug flow with different recurrence frequencies in cases of large Q_(g)/Q_(l).However,there is no mode switching in the out-of-plane response.The switching between the first and third modes for the in-plane response and the second and fourth modes for the out-of-plane response(MS II)occurs in cases of relatively high vm and low Q_(g)/Q_(l). 展开更多
关键词 slug-induced vibration slug characteristics mode switching flexible catenary riser
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Experimental and modeling investigation of zero net liquid flow in hilly terrain pipeline 被引量:1
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作者 Bo Huang Qiang Xu +4 位作者 Ying-Jie Chang Ye-Qi Cao Hai-Yang Yu Yu-Wen Li Lie-Jin Guo 《Petroleum Science》 2025年第5期2183-2202,共20页
Hilly terrain pipeline is a common form of pipeline in oil and gas storage and transportation industry.Due to the hilly terrain influence, the liquid at the elbow of the gathering pipeline is easy to flow back and acc... Hilly terrain pipeline is a common form of pipeline in oil and gas storage and transportation industry.Due to the hilly terrain influence, the liquid at the elbow of the gathering pipeline is easy to flow back and accumulate to form slug flow, so it is necessary to remove the accumulated liquid by gas purging. In this paper, experiment is carried out in hilly terrain pipelines. Three flow patterns of stratified flow, slug flow and stratified entrained flow are observed. The process of gas purging accumulated liquid is divided into four stages, namely liquid accumulation, liquid rising, continuous outflow and tail outflow. At the same time, the flow pattern maps of each stage are drawn. The pressure drop signal is analyzed in time domain and frequency domain, and the contour map of pressure drop distribution is drawn. It is found that the ratio of range to average value can well distinguish the occurrence range of each flow pattern.Based on visualization, the transition process of slug flow to stratified flow and stratified entrained flow is studied, and the transition boundary prediction model is established. An image processing method is proposed to convert the image signal into a similarity curve, and PSD analysis is performed to calculate the slug frequency. The normal distribution is used to fit the slug frequency, and the predicted correlation is in good agreement with the experimental data. 展开更多
关键词 Hilly terrain pipeline Zero net liquid flow Slug flow Flow pattern transition Quantitative image processing
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基于网络药理学及细胞实验探析乌附理中汤治疗胃癌的作用机制
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作者 马祺 杨斌锋 +1 位作者 乔玉洁 黄邦荣 《西部中医药》 2025年第4期33-41,共9页
目的:利用网络药理学和细胞实验探究乌附理中汤治疗胃癌的作用机制。方法:通过网络药理学预测乌附理中汤的有效成分,核心靶点和信号通路,选用胃癌细胞HGC27细胞、MKN28细胞通过CCK-8实验、RT-qPCR实验、Western blot实验、细胞划痕实验... 目的:利用网络药理学和细胞实验探究乌附理中汤治疗胃癌的作用机制。方法:通过网络药理学预测乌附理中汤的有效成分,核心靶点和信号通路,选用胃癌细胞HGC27细胞、MKN28细胞通过CCK-8实验、RT-qPCR实验、Western blot实验、细胞划痕实验验证网络药理学预测的主要靶点以探究乌附理中汤治疗胃癌的作用机制。结果:乌附理中汤共含活性成分198个,对应靶点884个;从GEO数据库中共检索到胃癌相关差异基因919个,获取交集靶点61个,其中MMP9、OLR1、PTGS2、MMP12、IL-6、SELE、MMP7、NOX4等为核心靶点;富集分析显示交集靶点主要富集在PI3K/AKT、AGE-RAGE、TNF、cAMP、NF-kappa B、IL-17、cGMP-PKG等信号通路。CCK-8、划痕实验提示,乌附理中汤能明显抑制HGC27细胞和MKN28细胞的增殖、迁移及侵袭能力(P<0.05)。RT-qPCR实验结果表明,乌附理中汤能降低SLUG、MMP9、Vimentin、VEGF mRNA表达水平(P<0.05)。Western blot实验结果表明乌理中汤能降低p-AKT、SLUG、MMP9、Vimentin、VEGF蛋白表达水平(P<0.05)。结论:乌附理中汤通过抑制AKT/GSK-3β/Snail(Slug)信号通路达到抑制胃癌的目的。 展开更多
关键词 胃癌 乌附理中汤 网络药理学 AKT/GSK-3β/Snail(Slug)信号通路
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罕见彩虹海蛞蝓现身德文郡
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作者 袁丹纯 《疯狂英语(新悦读)》 2025年第1期21-24,75,共5页
1 A rare and vibrant sea slug,a vision of beauty with its rainbow colors,has been discovered in the waters of Devon,a first⁃time occurrence that has sparked both awe and concern.Felix Lever and her father Ashley were ... 1 A rare and vibrant sea slug,a vision of beauty with its rainbow colors,has been discovered in the waters of Devon,a first⁃time occurrence that has sparked both awe and concern.Felix Lever and her father Ashley were exploring the rock pools near Wembury,South Devon,when they came across this remarkable find. 展开更多
关键词 DISCOVERY rock pools sea slug sea sluga RARE rainbow colored devon
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Revisiting Taylor bubble motion in an oscillating vertical tube
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作者 Quan Yao Guangzhao Zhou 《Theoretical & Applied Mechanics Letters》 2025年第3期238-245,共8页
In this study,we numerically investigate the rise of a Taylor bubble in a vertically oscillating round tube.The results show that increasing the oscillation frequency and amplitude reduces the bubble rise velocity,whi... In this study,we numerically investigate the rise of a Taylor bubble in a vertically oscillating round tube.The results show that increasing the oscillation frequency and amplitude reduces the bubble rise velocity,which is consistent with previously reported experimental findings.Analysis of the flow in the annular film region indicates that the influence of tube wall oscillations is minimal.This suggests that the effect of tube oscillations is essentially equivalent to that of an oscillating piston above the bubble,leading to a similar mechanism for bubble deceleration.Using a theoretical formula from the literature,we demonstrate that at sufficiently high frequencies,the amplitude of the tube velocity oscillations becomes the sole control parameter affecting bubble deceleration.This study enhances our understanding of Taylor bubble behavior in mechanically oscillating environments and provides useful insights into the design of control strategies for Taylor bubble motion in vertical slug flows. 展开更多
关键词 Taylor bubble Slug flow Tube oscillation Flow control
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Multiphase Vertical Slug Flow Hydrodynamics with Hydrate Phase Transition
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作者 WANG Yangyang LIANG Weixing +1 位作者 LOU Min WANG Yu 《Journal of Ocean University of China》 2025年第4期941-953,共13页
Hydrate phase transition may pose risks in pipeline blockage and severe challenges for offshore natural gas hydrate pro-duction.The present work involves the development of a multiphase gas-liquid-solid vertical slug ... Hydrate phase transition may pose risks in pipeline blockage and severe challenges for offshore natural gas hydrate pro-duction.The present work involves the development of a multiphase gas-liquid-solid vertical slug flow hydrodynamic model consi-dering hydrate phase transition kinetics with heat and mass transfer behaviors.The varying gas physical properties due to pressure and temperature variations are also introduced to evaluate vertical slug flow characteristics.The proposed model is used to carry out a series of numerical simulations to examine the interactions between hydrate phase transition and vertical slug flow hydrodynamics.Furthermore,the hydrate volumetric fractions under different pressure and temperature conditions are predicted.The results reveal that hydrate formation and gas expansion cause the mixture superficial velocity,and the gas and liquid fractions,void fraction in liq-uid slug,and unit length tend to decrease.The increase in outlet pressure leads to an increased hydrate formation rate,which not only increases the hydrate volumetric fraction along the pipe but also causes the upward shift of the hydrate phase transition critical point. 展开更多
关键词 vertical slug flow hydrate phase transition heat and mass transfer flow assurance multiphase flow modeling
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宫颈癌中Snail、Slug和KAI1的表达及临床意义 被引量:16
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作者 龚晓萌 陶仪声 +5 位作者 周蕾 俞岚 武世伍 宋文庆 王丹娜 承泽农 《南方医科大学学报》 CAS CSCD 北大核心 2015年第12期1733-1738,共6页
目的检测宫颈癌中上皮间充质转化(EMT)相关标记物Snail、Slug和转移抑制因子KAI1的表达,并分析它们的相互关系及其临床病理意义。方法利用免疫组化Elivision TM plus法检测154例宫颈鳞状细胞癌(CSCC)、50例宫颈上皮内瘤变(CIN)和40例正... 目的检测宫颈癌中上皮间充质转化(EMT)相关标记物Snail、Slug和转移抑制因子KAI1的表达,并分析它们的相互关系及其临床病理意义。方法利用免疫组化Elivision TM plus法检测154例宫颈鳞状细胞癌(CSCC)、50例宫颈上皮内瘤变(CIN)和40例正常宫颈上皮组织中Snail、Slug和KAI1蛋白的表达。结果 Snail、Slug和KAI1在CSCC组、CIN组和对照组的阳性表达率分别为66.2%、66.9%、43.5%;32.0%、34.0%、64.0%和0%、2.5%、95.0%,两组相互之间差异有统计学意义(P<0.05)。Snail、Slug和KAI1蛋白的表达均与CSCC肿瘤组织的分化程度、淋巴结转移与否、浸润深度、FIGO分期及术后生存期均有关(P<0.05);Snail蛋白的表达与Slug蛋白的表达呈正相关关系(r=0.752,P<0.001),Snail和Slug蛋白的表达均与KAI1蛋白的表达呈负相关关系(P<0.001)。Kaplan-Meier生存分析表明,Snail和Slug蛋白表达阳性的患者其生存率明显低于其阴性表达的患者(P<0.001);KAI1蛋白表达阳性的患者其生存率明显高于其阴性表达的患者(P<0.001)。Cox回归分析显示Snail和KAI1蛋白的阳性表达及FIGO分期是影响CSCC患者术后生存的独立预后因素。结论 Snail、Slug和KAI1蛋白的表达水平在CSCC组织中与肿瘤组织的分化程度、FIGO、浸润深度、淋巴结转移与否及预后等均有关;在CSCC组织中早期联合检测Snail、Slug和KAI1对判断预后有价值。 展开更多
关键词 宫颈肿瘤 SNAIL SLUG KAI1 预后
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HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义 被引量:14
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作者 龙方懿 印国兵 +5 位作者 刘智敏 杨俊艳 贾朝莉 刘小花 董超然 王旎 《中国免疫学杂志》 CAS CSCD 北大核心 2012年第6期502-506,共5页
目的:探讨HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义。方法:采用免疫组化SP法,检测术前未使用放疗、化疗及激素治疗的69例乳腺癌组织中HIF-1α、MT、Slug和E-cadherin的表达情况。采用RT-PCR方法检测乳腺癌细胞系MCF-7... 目的:探讨HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义。方法:采用免疫组化SP法,检测术前未使用放疗、化疗及激素治疗的69例乳腺癌组织中HIF-1α、MT、Slug和E-cadherin的表达情况。采用RT-PCR方法检测乳腺癌细胞系MCF-7和MDA-MB-231中HIF-1α、MT、Slug和E-cadherin mRNA的表达情况。结果:免疫组化结果表明:在69例乳腺癌组织中,HIF-1α、MT、Slug和E-cadherin的阳性率分别为71.01%、42.03%、43.48%和50.72%。HIF-1α、Slug和E-cad-herin的表达对于TNM分期和腋淋巴结转移有显著性差异(P<0.05);MT的表达对于TNM分期有显著性差异(P<0.05)。在69例乳腺癌组织标本中HIF-1α与MT表达呈显著正相关(r=0.285,P<0.05),MT与Slug呈显著正相关(r=0.379,P<0.01),Slug与E-cadherin呈显著负相关(r=-0.422,P<0.01)。RT-PCR半定量结果显示:HIF-1α、MT、Slug和E-cadherinmRNA在人乳腺癌细胞系MCF-7和MDA-MB-231中均有表达,但在恶性程度更高的MDA-MB-231细胞中HIF-1α、MT、Slug表达较高,而E-cadherin表达较低,差异具有显著性(P<0.05)。结论:HIF-1α、MT、Slug和E-cadherin在乳腺癌中的表达情况可作为监测乳腺癌发展的指标。 展开更多
关键词 乳腺癌 HIF-1Α MT SLUG E-CADHERIN
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Claudin-7与Slug在肺鳞癌和腺癌中的表达及其临床意义 被引量:14
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作者 李锐 张道荣 +1 位作者 蔡存伟 董经宇 《中国肺癌杂志》 CAS 2011年第6期492-496,共5页
背景与目的 Claudins是紧密连接的骨架蛋白,Claudin-7是Claudins家族成员之一。本研究旨在观察Claudin-7和Slug在肺鳞癌和腺癌中的表达及其与临床病理因素的关系,并探讨Claudin-7和Slug的相互关系。方法采用免疫组织化学SP法检测101例... 背景与目的 Claudins是紧密连接的骨架蛋白,Claudin-7是Claudins家族成员之一。本研究旨在观察Claudin-7和Slug在肺鳞癌和腺癌中的表达及其与临床病理因素的关系,并探讨Claudin-7和Slug的相互关系。方法采用免疫组织化学SP法检测101例原发性肺鳞癌、腺癌组织中Claudin-7和Slug的表达,同时应用Westernblot检测30例新鲜肺癌组织及其配对的癌旁组织中Claudin-7和Slug的表达情况。结果 Claudin-7在肺癌中的表达明显低于正常肺组织,并且与分化程度和淋巴结转移有关(P<0.05),Slug在肺癌中的表达明显高于正常肺组织,除与分化程度和淋巴结转移有关外,还与TNM分期有关(P<0.05),肺鳞癌、腺癌中Claudin-7与Slug的表达具有负相关性(r=-0.566,8)。结论肺鳞癌、腺癌中Claudin-7的低表达与Slug的高表达可能是肺组织恶性转变和转移的有关标志物之一。 展开更多
关键词 肺肿瘤 CLAUDIN SLUG 紧密连接
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EMT分子标志物在肝癌细胞系中的表达及其意义 被引量:10
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作者 贾皑 张璐 +3 位作者 任莉 范修德 秦洁 罗培培 《西安交通大学学报(医学版)》 CAS CSCD 北大核心 2019年第4期537-541,共5页
目的探讨人肝癌细胞系EMT相关分子标志物的表达情况,为确定相关的肝癌细胞研究模型奠定基础。方法利用实时定量PCR方法检测5株人肝癌细胞系(SNU368、SNU739、HLF、Huh-1和MHCC97L)中E-cadherin、ZO-1、N-cadherin、Vimentin、Snail和Slu... 目的探讨人肝癌细胞系EMT相关分子标志物的表达情况,为确定相关的肝癌细胞研究模型奠定基础。方法利用实时定量PCR方法检测5株人肝癌细胞系(SNU368、SNU739、HLF、Huh-1和MHCC97L)中E-cadherin、ZO-1、N-cadherin、Vimentin、Snail和Slug的mRNA表达,Westernblot和免疫荧光检测E-cadherin、ZO-1、N-cadherin、Vimentin、Snail和Slug的蛋白表达。结果E-cadherin、ZO-1、N-cadherin、Vimentin、Snail和Slug的mRNA在5种肝癌细胞中均有不同程度表达,E-cadherin、ZO-1、N-cadherin和Slug蛋白在5个细胞株中亦有表达,Vimentin蛋白主要在SNU368、SNU739、HLF和MHCC97L细胞中表达明显,Snail蛋白在SNU368,SNU739,Huh-1和MHCC97L细胞中表达较高。结论HLF细胞系表现出更明显的间质细胞表型特点,Huh-1和SNU368则表现为较典型的上皮细胞表型特点。 展开更多
关键词 肝细胞肝癌 上皮-间质转化(EMT) E-CADHERIN SNAIL SLUG
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