Flower and fruit abscission reduce crop yield,so decreasing abscission is a significant agricultural issue.HAESA(HAE)and HAESA-like2(HSL2)kinases and their ligand,INFLORESCENCE DEFICIENT IN ABSCISSION(IDA)peptide,have...Flower and fruit abscission reduce crop yield,so decreasing abscission is a significant agricultural issue.HAESA(HAE)and HAESA-like2(HSL2)kinases and their ligand,INFLORESCENCE DEFICIENT IN ABSCISSION(IDA)peptide,have been confirmed to be the core elements regulating floral organ abscission in Arabidopsis thaliana.Our earlier research revealed that Sl IDL6,a homolog of IDA in tomato,functions similarly to At IDA,regulating the abscission of tomato flower organs.Here,we further isolated three HAESA-like homologs,Sl HSL1/2/3,which are involved in tomato flower abscission.Sl HSL1/2/3 are highly expressed in the abscission zone(AZ).The knockout mutant lines of Slhsl1,Slhsl2,and Slhsl3 showed lower flower pedicel abscission than wild type(WT).The double mutant of Slhsl1Slhsl2,Slhsl1Slhsl3,and Slhsl2Slhsl3 further depressed abscission than each of the single mutant lines,while triple mutants Slhsl1Slhsl2Slhsl3 exhibited the lowest abscission,indicating that Sl HSL1/2/3 mediated abscission is non-redundancy,at least partially.Treating tomato pedicel explants with Sl IDL6 peptide significantly accelerated pedicel abscission in WT.However,it had little effect on the abscission rate of Sl HSL1/2/3 knockout lines,indicating that Sl HSL1/2/3 are the receptors of Sl IDL6 in pedicel abscission.Ethylene action inhibitor 1-methylcyclopropene(1-MCP)can significantly depress the expression of Sl HSL1/2/3.Ethylene can significantly accelerate the abscission of WT,while less abscission was found in Sl HSL1/2/3 knockout lines.Our findings indicate that Sl HSL1/2/3 can act as receptors for Sl IDL6 to positively regulate tomato pedicel abscission,and the abscission regulated by Sl HSL1/2/3 was partially dependent on ethylene.展开更多
基金supported by the Liaoning Science and Technology Innovation Team Project,China(JYTTD2024007)。
文摘Flower and fruit abscission reduce crop yield,so decreasing abscission is a significant agricultural issue.HAESA(HAE)and HAESA-like2(HSL2)kinases and their ligand,INFLORESCENCE DEFICIENT IN ABSCISSION(IDA)peptide,have been confirmed to be the core elements regulating floral organ abscission in Arabidopsis thaliana.Our earlier research revealed that Sl IDL6,a homolog of IDA in tomato,functions similarly to At IDA,regulating the abscission of tomato flower organs.Here,we further isolated three HAESA-like homologs,Sl HSL1/2/3,which are involved in tomato flower abscission.Sl HSL1/2/3 are highly expressed in the abscission zone(AZ).The knockout mutant lines of Slhsl1,Slhsl2,and Slhsl3 showed lower flower pedicel abscission than wild type(WT).The double mutant of Slhsl1Slhsl2,Slhsl1Slhsl3,and Slhsl2Slhsl3 further depressed abscission than each of the single mutant lines,while triple mutants Slhsl1Slhsl2Slhsl3 exhibited the lowest abscission,indicating that Sl HSL1/2/3 mediated abscission is non-redundancy,at least partially.Treating tomato pedicel explants with Sl IDL6 peptide significantly accelerated pedicel abscission in WT.However,it had little effect on the abscission rate of Sl HSL1/2/3 knockout lines,indicating that Sl HSL1/2/3 are the receptors of Sl IDL6 in pedicel abscission.Ethylene action inhibitor 1-methylcyclopropene(1-MCP)can significantly depress the expression of Sl HSL1/2/3.Ethylene can significantly accelerate the abscission of WT,while less abscission was found in Sl HSL1/2/3 knockout lines.Our findings indicate that Sl HSL1/2/3 can act as receptors for Sl IDL6 to positively regulate tomato pedicel abscission,and the abscission regulated by Sl HSL1/2/3 was partially dependent on ethylene.
