Aging is a persistent topic of interest,with skin aging as its most visible manifestation,characterized by a reduction in intact collagen and elastic fibers in the dermis.Hyaluronic acid,a vital component of the extra...Aging is a persistent topic of interest,with skin aging as its most visible manifestation,characterized by a reduction in intact collagen and elastic fibers in the dermis.Hyaluronic acid,a vital component of the extracellular matrix present in the skin,has become a mainstream method for skin rejuvenation through injections.However,the rapid degradation of pure hyaluronic acid,combined with insufficient maintenance duration and often limited therapeutic effects,presents significant challenges for injectable treatments.Additionally,low patient compliance due to discomfort from needles penetrating the dermal layer further complicates its use.In this review,we summarize and compare existing interventions for skin aging,focusing on strategies to prolong the degradation cycle of hyaluronic acid,including variations in cross-linking modalities and injection techniques.We conclude that the injection of cross-linked modified hyaluronic acid via microneedles represents a promising approach to extend the degradation cycle,offering valuable insights for current therapeutic strategies.展开更多
BACKGROUND Research has been increasingly conducted on the connection between mesenchymal stem cell(MSC)-conditioned medium(MSC-CM)and aging.However,most studies have focused on adipose-derived MSC-CM(ADMSC-CM),result...BACKGROUND Research has been increasingly conducted on the connection between mesenchymal stem cell(MSC)-conditioned medium(MSC-CM)and aging.However,most studies have focused on adipose-derived MSC-CM(ADMSC-CM),resulting in a research bias.We hypothesized that umbilical cord-derived MSCs,being younger than adipose-derived MSCs,would be more suitable for overcoming aging-related processes.AIM To assess the efficacy and safety of umbilical cord-derived MSC-CM(UCMSCCM)for preventing and treating skin aging.METHODS In vitro and in vivo studies were conducted to compare UCMSC-CM with ADMSC-CM,the most studied active aging-preventive conditioned medium to date.Additionally,the most effective delivery method of UCMSC-CM for aged skin was identified.RESULTS UCMSC-CM had a higher content of effective factors,stimulated higher proliferation of fibroblasts,and strongly inhibited melanin production in B16F1 cells.In aged mice,UCMSC-CM application increased skin thickness,the number of Ki-67-positive cells,and the area of collagen deposition.UCMSC-CM was more effective than ADMSC-CM in preventing and treating skin aging.Additionally,a safety evaluation of UCMSC-CM performed in various animal models indicated that it was safe even when used directly on the skin.CONCLUSION UCMSC-CM is effective and safe for preventing and treating skin aging.展开更多
Objective： To investigate cutaneous aging patterns of residents in Hangzhou, Zhejiang, China, and their contributing factors. Methods： Eight hundred and forty-eight Hangzhou residents received the survey between Mar...Objective： To investigate cutaneous aging patterns of residents in Hangzhou, Zhejiang, China, and their contributing factors. Methods： Eight hundred and forty-eight Hangzhou residents received the survey between March 2004 and September 2004. Results： Facial wrinkling first occurred at 21 years of age and skin elasticity began to lose at 22 years of age. In middle-aged and old people, facial wrinkling and looseness escalated with the increase of ultraviolet （UV）-exposure time, indicating the accelerating effect of a higher accumulative dose of UV radiation on skin aging. Only Fitzpatrick types Ⅱ, Ⅲ and Ⅳ were found in the skin phototypes of residents in Hangzhou area, and Fitzpatrick type Ⅱ seemed to be much more subject to severe wrinkling, elasticity destruction and skin tumors than types Ⅲ and Ⅳ. The oily skin was more protected against wrinkling and facial looseness than dry skin. However, as to concomitant cutaneous diseases, no difference was found among different skin types. Conclusion： Age, solar-exposure time, Fitzpatrick type and skin type are the associated forces in promoting skin aging, and emotional factor seems to be another independent risk factor. The age of 49 years and 2 h/d of solar-exposure time seem to be the turning points responsible for dramatic changes of cutaneous appearance in the process of skin aging in Southeast China.展开更多
Background:Oxidative stress is a significant factor in skin aging and pigmentation,which can be precipitated by various circumstances.Antioxidants and tyrosinase inhibitors,such as carotenoids,yeast extract(glutathion...Background:Oxidative stress is a significant factor in skin aging and pigmentation,which can be precipitated by various circumstances.Antioxidants and tyrosinase inhibitors,such as carotenoids,yeast extract(glutathione),sodium hyaluronate,astaxanthin,and niacin,can individually protect the skin against aging through distinct mechanisms.These mechanisms potentially enhance the skin barrier and improve signs of aging and pigmentation.However,the synergistic effects of these compounds,as found in a golden tomato extract formulation,have been scarcely explored.Objective:To evaluate the effects of an orally administered formulation on the skin aging and pigmentation.Material and Methods:In this study,a randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLAB?Tomato Niacinamide beverage.Out of all participants,62 volunteers completed the experiment and were included in the statistical analysis.Results:The results indicated that after eight weeks of consuming the research product,there were no significant changes in the skin indicators within the placebo group.In contrast,the treatment group receiving the sample formulation exhibited a 35.63%increase in stratum corneum hydration and a 29.39%reduction in transepidermal water loss(TEWL),suggesting enhanced skin hydration.Visual assessments revealed improvements in skin color and gloss index by 15.03%and 11.41%,respectively,in the treatment group.Furthermore,the skin gloss and individual typology angle(ITA)value increased by 18.59%and 6.36%,respectively,leading to a lighter skin tone.Significant enhancements were also observed in skin pigmentation,color uniformity,and redness.After eight weeks of intervention with the sample,blood levels of superoxide dismutase(SOD)and glutathione peroxidase(GPx)increased,while malondialdehyde(MDA)levels decreased.Conclusion:These findings confirm that continuous intake of the tomato extract formulation over eight weeks effectively improved the volunteers'skin whitening and hydration,and visibly brightened skin tone through an antioxidant mechanism.展开更多
The“gut-skin”axis has been proved and is considered as a novel therapy for the prevention of skin aging.The antioxidant efficacy of oligomannonic acid(MAOS)makes it an intriguing target for use to improve skin aging...The“gut-skin”axis has been proved and is considered as a novel therapy for the prevention of skin aging.The antioxidant efficacy of oligomannonic acid(MAOS)makes it an intriguing target for use to improve skin aging.The present study further explored whereby MAOS-mediated gut-skin axis balance prevented skin aging in mice.The data indicated the skin aging phenotypes,oxidative stress,skin mitochondrial dysfunction,and intestinal dysbiosis(especially the butyrate and HIF-1a levels decreased)in aging mice.Similarly,fecal microbiota transplantation(FMT)from aging mice rebuild the aging-like phenotypes.Further,we demonstrated MAOS-mediated colonic butyrate-HIF-1a axis homeostasis promoted the entry of butyrate into the skin,upregulated mitophagy level and ultimately improving skin aging via HDAC3/PHD/HIF-1a/mitophagy loop in skin of mice.Overall,our study offered a better insights of the effectiveness of alginate oligosaccharides(AOS),promised to become a personalized targeted therapeutic agents,on gut-skin axis disorder inducing skin aging.展开更多
The skin is the largest organ of the human body,and its aging is visible to the naked eye.The aging rate of men and women is slightly different.This study compared the protein expression of skin samples on the curved ...The skin is the largest organ of the human body,and its aging is visible to the naked eye.The aging rate of men and women is slightly different.This study compared the protein expression of skin samples on the curved forearms of 11 healthy women and 9 healthy men.Quantitative proteomics analysis found that the expression of epidermal proteins in men and women of the same age group was different.Compared with female skin,in male skin,20 proteins were upregulated,and 7 proteins were downregulated.These data suggest that men and women have differences in the speed of skin aging.For the first time in this experiment,a non-invasive mass spectrometer was used to detect 27 different-related epidermal proteins between men and women.Compared with women,among the 20 epidermal proteins upregulated in men,their functions can be classified into antioxidants,epidermal lipid metabolism,signal transduction,membrane transport,and cell biological processes;the 7 downregulated proteins are involved in a variety of biological processes and inflammatory reactions.Our experiments have discovered epidermal proteins related to the differences between men and women,enriching the library of epidermal differential proteins between men and women and enriching the mechanism of skin aging between men and women from the perspective of epidermal differential proteins.展开更多
Background: Wrinkles, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Sulci cutis connecting to follicular orifices frequently form perifollicular cracks, which join together...Background: Wrinkles, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Sulci cutis connecting to follicular orifices frequently form perifollicular cracks, which join together adjacent sulci. This follicle-sulcus-crack complex (FSCC) is exacerbated by dehydration. Purpose: Although dehydration is associated with facial skin aging, FSCC’s implications in facial skin aging remain unresolved. Method: We measured facial skin parameters in 1999 and 2010 in 108 Japanese women, and in 2006 and 2016 in 182 Chinese women. We addressed whether expansion of FSCC is associated with other skin aging parameters. We also examined whether skin moisturizer can reverse the expanded FSCC. Results: In both clinical studies, FSCC severity as well as wrinkles, spots, and roughness significantly deteriorated with age. Notably, FSCC significantly increased as early as in subjects in their 20s, whereas wrinkles, spots, and roughness became conspicuous in those in their 40s or older. Moreover, subjects with more severe FSCC in the initial measurement exhibited significantly worse wrinkles, spots, and roughness in the second measurement a decade later. Dehydration was significantly correlated with expanded FSCC. Furthermore, FSCC expansion was reversed after appropriate moisturization by daily application of Galactomyces ferment filtrate (GFF)-containing skincare product (SK-II Skin Power Cream) for 4 weeks in 37 women. Conclusion: The expanded FSCC is likely to be an early indicator of facial skin aging. Appropriate moisturization may reduce FSCC formation and decelerate facial skin aging.展开更多
Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss...Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss (TEWL). Recent advances in coherence tomography have also revealed that skin aging affects in vivo epidermal keratinocyte architecture. However, the interconnectivity between spatial architectural aging and visual/physiological aging parameters remains largely unknown. Purpose: To elucidate whether the tomographic keratinocyte architectural aging is correlated with visual and physiological skin aging parameters and to quantitatively evaluate the improvements of the architectural, visual, and physiological aging parameters by the daily treatment of the skin care formula containing Galactomyces Ferment Filtrate (GFF, 8X Pitera<sup>TM</sup>). Method: We measured the in vivo keratinocyte cellular architecture with two-photon stereoscopic tomography obtaining by-layer epidermal section images in 78 Asian females of various ages. Visual aging parameters were analyzed using a portable image capture system. Hydration and TEWL were also assessed. The anti-aging effects of GFF-containing skin moisturizer (SK-II LXP Cream<sup>TM</sup>) were also examined in two studies after twice-daily application for 2 (N = 35) and 4 (N = 32) weeks. Results: As for the keratinocyte cellular architecture, skin aging was significantly associated with decreased cell density and increased cell uniformity. These architectural aging parameters were significantly correlated with visual and physiological aging parameters, namely, rough texture, wrinkles, pore dilation, dull skin tone, dehydration, and increased TEWL. The strong interconnectivity allowed us to develop formulae to estimate the keratinocyte architecture from visual aging parameters. Moreover, twice-daily application of SK-II significantly improved the keratinocyte architecture associated with multiple skin aging visual and physiological parameters. Conclusion: Skin aging is a process involving mutual interconnections among epidermal keratinocyte cellular architecture, visual, and physiological parameters. The GFF-containing moisturizer SK-II effectively improves spatial architecture of keratinocytes in epidermis and these evaluated skin aging parameters in a new trajectory over the course of treatment. .展开更多
This paper briefly introduce the test method for human evaluation of cosmetics to delay skin aging,and provide reference for the test of relevant indexes.By formulating a reasonable test plan and choosing appropriate ...This paper briefly introduce the test method for human evaluation of cosmetics to delay skin aging,and provide reference for the test of relevant indexes.By formulating a reasonable test plan and choosing appropriate equipment to test the improvement of the skin aging characteristics before and after the product is used,it can provide a scientific basis for claiming the effect of delaying skin aging.展开更多
Skin aging is an unavoidable aspect of human life.Premature skin aging can result from poor care,environmental pollutants,and ultraviolet radiation exposure.Wrinkles,lines,spots,uneven skin tone,and pigmentation are o...Skin aging is an unavoidable aspect of human life.Premature skin aging can result from poor care,environmental pollutants,and ultraviolet radiation exposure.Wrinkles,lines,spots,uneven skin tone,and pigmentation are often indicators of skin aging.One cannot avoid aging but cosmetics and pharmaceutical approaches can minimize and delay the damage.Topical applications of biocompatible and biodegradable vehicles have been explored for delivering anti-aging compounds.Lecithin organogel(LO)is an effective vehicle for topical delivery of many bioactive agents used in aging treatment.Lecithin is cell component isolated from soya beans or eggs and purified to show excellent gelation in non-polar solvents when combined with water.LO can form a heat-stable,resistant to microbial growth,visco-elastic,optically transparent,and non-birefringent micellar system.It serves as an organic medium to enhance dermal permeation of poorly permeable drugs by effectively partitioning into the skin.Its ability to dissolve in hydrophilic as well as in lipophilic drugs makes it a dynamic vehicle,which can be explored as a carrier for anti-aging agents.展开更多
Owing to its constant exposure to the external environment and various stimuli,skin ranks among the organs most vulnerable to manifestations of aging.Preventing and delaying skin aging has become one of the prominent ...Owing to its constant exposure to the external environment and various stimuli,skin ranks among the organs most vulnerable to manifestations of aging.Preventing and delaying skin aging has become one of the prominent research subjects in recent years.Mesenchymal stem celis(MsCs)are multipotent stem cells derived from mesoderm with high self-renewal ability and multilineage differentiation potential.MSC-derived extracellular vesicles(MSC-EVs)are nanoscale biological vesicles that facilitate intercellular communication and regulate biological behavior.Recent studies have shown that MSC-EVs have potential applications in anti-aging therapy due to their anti-inflammatory,anti-oxidative stress,and wound healing promoting abilities.This review presents the latest progress of MSC-EVs in delaying skin aging.It mainly includes the MSC-EVs promoting the proliferation and migration of keratinocytes and fibroblasts,reducing the expression of matrix metalloproteinases,resisting oxidative stress,and regulating inflammation.We then briefly discuss the recently discovered treatment methods of MSc-EVs in the field of skin anti-aging.Moreover,the advantages and limitations of EV-based treatments are also presented.展开更多
Air pollution adversely affects skin,leading to skin inflammation and premature skin aging.Plant derived antioxidant compounds have been considered to be promising in discovery of effective agents for the protection o...Air pollution adversely affects skin,leading to skin inflammation and premature skin aging.Plant derived antioxidant compounds have been considered to be promising in discovery of effective agents for the protection of skin from the damage by air pollutants.Our previous studies demonstrated that Averrhoa carambola fruit(known as star fruit)is rich in flavonoid C-glycosides with unique structures and potent antioxidant activity.Thus,the star fruit extract(SFE)and main flavonoid C-glycoside components,carambolasides I,J,and P(1-3),carambolaflavone B(4),and isovitexin 2″-O-α-l-rhamnoside(5),were investigated for the activity against air pollutant stress in human epidermis.As a result,SFE and compounds 1-5 exhibited significant inhibitory activity against protein carbonylation in oxidative-stressed stratum corneum with the best activity being shown by compound 3.SFE and compounds 2-5 were also active against engine exhaust-induced protein carbonylation in stratum corneum.When further evaluated,SFE and com-pound 3 significantly inhibited gene expression of the key inflammation mediators IL-1αand COX-2 in PM-stressed keratinocytes.The results indicated that SFE and the flavonoid C-glycosides are potentially effective against air pollutant-induced skin inflammation and premature aging.展开更多
Objective： To observe the effect of acupuncture on skin aging of senile rats and discuss the mechanism of acupuncture in treating skin aging. Methods： The 18-month-old senile Wistar rats were randomly divided into t...Objective： To observe the effect of acupuncture on skin aging of senile rats and discuss the mechanism of acupuncture in treating skin aging. Methods： The 18-month-old senile Wistar rats were randomly divided into three groups, a blank group, a surrounding needling group, a surrounding needling plus Zusanli （ST 36） group, comparing with the 8-month-old young rats. The tests included the content of dissoluble hydroxyproline （Hyp） and the activity of superoxide dismutase （SOD） in the skin of rats＇ abdomen. The transmission electron microscope was used for the morphological observation of the fibroblasts and the arrangement of the collagen fiber. Results： The dermal organelles in the fibroblasts of the rats decreased with the cellular structures. Meanwhile, the spaces among the collagen fibers extended and the aging bridging increased. The surrounding needling improved the activity of the fibroblasts. The dissoluble Hyp content in the surrounding needling group was much higher than the rats in the blank group, but the SOD activity didn＇t change significantly. However, the surrounding needling plus Zusanli （ST 36） enhanced the SOD activity. Conclusion： Acupuncture may improve the activity of the fibroblasts in the skin to increase the dissoluble collagen content, resulting in the improvement of an aged appearance. Surrounding needling plus needling Zusanli （ST 36） could enhance the SOD activity.展开更多
Skin aging and wound healing have always been significant topics in the skin field.Hyaluronic acid(HA),a natural glycosaminoglycan(GAG)presenting in the extracellular matrix(ECM),is extensively applied in the fields o...Skin aging and wound healing have always been significant topics in the skin field.Hyaluronic acid(HA),a natural glycosaminoglycan(GAG)presenting in the extracellular matrix(ECM),is extensively applied in the fields of anti-aging therapeutics and wound repair due to its unique physicochemical and biological properties.HA is indispensable in the regulation of diverse biological processes such as cellular proliferation,differentiation,migration,signaling,inflammatory responses,angiogenesis,and tissue regeneration.Consequently,HA-based formulations,including creams,gels,serums,and dermal fillers,are instrumental in combating wrinkles,enhancing skin hydration,correcting tear trough deformity,and restoring facial volume.Additionally,the inherent biocompatibility and high biodegradability of HA facilitates its chemical modification,rendering it an ideal material for wound healing and tissue regeneration.This review summarizes the recent advances and applications of the molecular mechanisms of HA and its derivatives in the field of anti-aging and wound healing;and discusses the prospects and challenges of HA-based compounds in tissue repair and skin regeneration.展开更多
<strong>Background:</strong> Young and intact skin is highly responsive. As skin ages, its responsiveness decreases. Production of structural components of the skin slows down and signs of skin aging appea...<strong>Background:</strong> Young and intact skin is highly responsive. As skin ages, its responsiveness decreases. Production of structural components of the skin slows down and signs of skin aging appear. External influences like UV radiation and pollution contribute to signs of skin aging. They lead to an increased formation of so-called free radicals. Bioactive antioxidant compounds, such as polyphenols, have beneficial effects on skin health by free radical scavenging. The purpose of this study was to evaluate the topical effects of new and highly concentrated resveratrol containing emulsion (Medskin Solutions Dr. Suwelack AG, 2% trans-resveratrol) on age-related alterations to the skin. <strong>Methods:</strong> A clinical observation study was performed on 20 subjects to observe the effects of a 2% resveratrol emulsion on typical signs of skin aging. Product was applied once daily for a period of 8 weeks in combination with a standardized treatment. Tested parameters were skin elasticity, skin barrier function, skin smoothness, skin thickness and density. <strong>Results:</strong> After 8 weeks of treatment, skin pH value is significantly lowered and skin barrier function improved. The value of skin elasticity (+5.3%), and skin density (+ 10.7%) had increased, while skin roughness (-6.4%) and skin dispensability (-45.9%) had diminished. Intensity of skin redness had decreased. Furthermore, expert and participant evaluations show satisfaction, especially with regard to skin smoothness and skin moisture. Conclusion: The resveratrol emulsion shows positive outcomes by supporting reduction of skin wrinkling and increasing skin firmness, as well as reducing skin redness, and is therefore an all-round anti-aging treatment.展开更多
Anti-aging research has become a popular scientific field with the increasing prominence of population aging.Rare ginsenoside Compound K(CK)has attracted widespread attention as an emerging anti-aging active ingredien...Anti-aging research has become a popular scientific field with the increasing prominence of population aging.Rare ginsenoside Compound K(CK)has attracted widespread attention as an emerging anti-aging active ingredient.The anti-aging effect of ginsenosides is considered to be one of the important roles of ginsenosides,and Compound K,as the main deglycosylated metabolite of ginsenosides,has a comprehensive anti-aging effect as a highly active ingredient obtained by transformation under the action of microbiota.Recent studies have shown that ginsenosides have anti-photo-oxidation,anti-skin aging,free radical scavenging and immunostimulatory effects,which can effectively prevent skin photoaging.With the progress of modern natural medicine extraction technology and the deepening of the research on the anti-skin aging of ginsenosides'high active ingredients,it will promote the development and application of natural product protective skin photoaging preparations.The rare ginsenoside Compound K plays an important role in the improvement of skin health and anti-aging,which is mainly realized by increasing the activity of antioxidant enzymes,inducing the expression of related genes,reducing the content of oxidative damage substances,regulating the immune system,and influencing the expression of cell-cycle regulators and aging genes.A more comprehensive and in-depth study of the molecular mechanism of the anti-aging effect of rare ginsenoside Compound K will be one of the focuses of future research.展开更多
Background: As the population of people aged 65 and older increases, greater attention is being paid to issues surrounding skin aging. This study was conducted to evaluate the efficacy of an oral unhydrolyzed eggshell...Background: As the population of people aged 65 and older increases, greater attention is being paid to issues surrounding skin aging. This study was conducted to evaluate the efficacy of an oral unhydrolyzed eggshell membrane (ESM) ingredient (branded Ovolux™) versus placebo on both objective and subjective markers of skin aging in middle-aged and senior adults, and also to assess the possible benefits of Ovolux™ on the health of hair and fingernails. Methods: Sixty-three healthy, men and women (aged 35 - 66) were randomly assigned to receive either oral ESM 300 mg (n = 32) or placebo (n = 31) once daily for 12 weeks. The primary endpoint was any statistically significant improvement in skin elasticity in the anterior region of the temporal fossa of the face versus placebo evaluated at 12 weeks of treatment based upon measurements taken with the Cutometer® MPA 580 multi-probe system. Secondary endpoints were improvement in skin firmness, viscoelasticity, hydration, or trans-epidermal water loss versus placebo. Additional secondary endpoints were any improvement in skin, hair, or fingernail subjective parameters versus placebo as determined via Patient’s Assessments. Results: Supplementation with Ovolux™ ESM produced a significant absolute treatment effect (TEabs) versus placebo after both six weeks (TEabs +8.4%, p = 0.019) and twelve weeks of treatment (TEabs +12.2%, p = 0.002) for the primary outcome measure total elasticity (R2). There were also significant treatment responses versus placebo for the objective measures of firmness (R0), net elasticity (R5), and viscoelasticity (R6) at both timepoints (R0: Week 6, −23.9%;Week 12, −16.8%) (R5: Week 6, +9.5%;Week 12, +10.6%) (R6: Week 6, −17.9%;Week 12, −17.7%), as well as for skin hydration versus placebo at both 6 weeks (+8.3%) and 12 weeks (+8.6%) of treatment. There were also significant improvements in the ESM treatment group versus placebo in the subjective measures of skin appearance, skin texture, overall skin health, hair texture, and overall hair health as determined by patient assessments. Conclusions: Ovolux™ provided significant broad-based improvements in critical biomechanical skin properties versus placebo, including elasticity, firmness, and viscoelasticity. These effects, along with improvement in skin hydration, were observed as early as 6 weeks and continued through the end of the 12-week study. Treatment with ESM was reported to be well-tolerated. Ovolux™ ESM appears to be a natural solution to help skin to resist the epidermal thickening, the dermal thinning, and the skin laxity and elastosis found in intrinsically aging skin. The Clinical Trial Registration number is: NCT06148337.展开更多
Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes ...Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR.展开更多
Each biological system possesses a widely unrecognized buffer system to maintain acid-base balance to a specific pH. The skin pH is crucial for physiological skin function. In aged or diseased skin, pH increase is obs...Each biological system possesses a widely unrecognized buffer system to maintain acid-base balance to a specific pH. The skin pH is crucial for physiological skin function. In aged or diseased skin, pH increase is observed and may negatively affect skin health. Skin care products with a pH that is slightly more acidic than the average normal skin pH and have an adequate buffering capacity, are considered beneficial for the skin. However, the buffer capacity of these products also plays an important role. In the present study, a possible normalization or acidification of skin surface pH and influence on skin hydration and skin barrier function was assessed via application of buffered skin care products that are formulated with pH ≤ 4.5. 48 subjects aged above 50 were treated with three different skin care products (Vitamin C Spheres, Collagen Spray, and Collagen Mask) and skin surface pH, skin hydration and barrier integrity were assessed before treatment start and after 4 weeks. The results show that after 4-week treatment with Vitamin C, the skin pH is acidified. Treatment with Collagen Spray and Collagen Mask showed maintenance of a physiological skin pH. Subgroup analysis of subjects that had a higher than average skin pH at study start demonstrated that all three tested skin care products were able to acidify the skin surface. In addition, skin hydration was also increased for two of the three tested products, whereas skin barrier is not significantly changed. This demonstrates that buffered skin care products formulated to a pH ≤ 4.5 are able to acidify and maintain physiological skin pH and may contribute to a physiological skin function.展开更多
Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in th...Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in the gut microbiota composition.However,the relationship between gut microbiota and skin aging remains unclear.In this study,we explored skin rejuvenation in aged mice through fecal microbiota trans-plantation(FMT)using feces from young mice.The results demonstrated enhanced water retention,thick-ened stratum corneum,increased collagen content,and improved epithelial cell differentiation in aged mice following FMT.Notably,FMT particularly increased the abundance of Lactobacillus and Lactococcus in aged mice,which were nearly undetectable in untreated aged mice.Non-targeted and targeted meta-bolomics analyses indicated that FMT significantly elevated levels of tryptophan(Trp)and its microbiota metabolites(e.g.,indole-3-lactic acid(ILA))in the feces and serum of aged mice.Both Trp and ILA appeared to rejuvenate aged skin by activating the aryl hydrocarbon receptor(AhR)to promote epidermal cell dif-ferentiation.In conclusion,FMT from young mice rejuvenated aged skin via Trp-metabolizing bacteria(Lactobacillus and Lactococcus)and Trp-derived metabolites,suggesting that interventions targeting Trp metabolites may effectively improve skin aging.展开更多
基金financial support from several corporate sponsors.Contributions were made by the National Natural Science Foundation of China(Grant No.32071332)Shenzhen Science and Technology Innovation Project(Grant No.JCYJ20210324095802006)+2 种基金Ningbo Bureau of Science and Technology(Grant No.2023Z187)TransEasy Medical Tech.Co.,Ltd.(Grant No.2021114)Shenzhen University MedTech Innovation Fund(2023YG027).
文摘Aging is a persistent topic of interest,with skin aging as its most visible manifestation,characterized by a reduction in intact collagen and elastic fibers in the dermis.Hyaluronic acid,a vital component of the extracellular matrix present in the skin,has become a mainstream method for skin rejuvenation through injections.However,the rapid degradation of pure hyaluronic acid,combined with insufficient maintenance duration and often limited therapeutic effects,presents significant challenges for injectable treatments.Additionally,low patient compliance due to discomfort from needles penetrating the dermal layer further complicates its use.In this review,we summarize and compare existing interventions for skin aging,focusing on strategies to prolong the degradation cycle of hyaluronic acid,including variations in cross-linking modalities and injection techniques.We conclude that the injection of cross-linked modified hyaluronic acid via microneedles represents a promising approach to extend the degradation cycle,offering valuable insights for current therapeutic strategies.
文摘BACKGROUND Research has been increasingly conducted on the connection between mesenchymal stem cell(MSC)-conditioned medium(MSC-CM)and aging.However,most studies have focused on adipose-derived MSC-CM(ADMSC-CM),resulting in a research bias.We hypothesized that umbilical cord-derived MSCs,being younger than adipose-derived MSCs,would be more suitable for overcoming aging-related processes.AIM To assess the efficacy and safety of umbilical cord-derived MSC-CM(UCMSCCM)for preventing and treating skin aging.METHODS In vitro and in vivo studies were conducted to compare UCMSC-CM with ADMSC-CM,the most studied active aging-preventive conditioned medium to date.Additionally,the most effective delivery method of UCMSC-CM for aged skin was identified.RESULTS UCMSC-CM had a higher content of effective factors,stimulated higher proliferation of fibroblasts,and strongly inhibited melanin production in B16F1 cells.In aged mice,UCMSC-CM application increased skin thickness,the number of Ki-67-positive cells,and the area of collagen deposition.UCMSC-CM was more effective than ADMSC-CM in preventing and treating skin aging.Additionally,a safety evaluation of UCMSC-CM performed in various animal models indicated that it was safe even when used directly on the skin.CONCLUSION UCMSC-CM is effective and safe for preventing and treating skin aging.
基金Project supported by the Skin Grant Program (2004) of L'Oreal-Chinese Medical Association (CMA), China
文摘Objective： To investigate cutaneous aging patterns of residents in Hangzhou, Zhejiang, China, and their contributing factors. Methods： Eight hundred and forty-eight Hangzhou residents received the survey between March 2004 and September 2004. Results： Facial wrinkling first occurred at 21 years of age and skin elasticity began to lose at 22 years of age. In middle-aged and old people, facial wrinkling and looseness escalated with the increase of ultraviolet （UV）-exposure time, indicating the accelerating effect of a higher accumulative dose of UV radiation on skin aging. Only Fitzpatrick types Ⅱ, Ⅲ and Ⅳ were found in the skin phototypes of residents in Hangzhou area, and Fitzpatrick type Ⅱ seemed to be much more subject to severe wrinkling, elasticity destruction and skin tumors than types Ⅲ and Ⅳ. The oily skin was more protected against wrinkling and facial looseness than dry skin. However, as to concomitant cutaneous diseases, no difference was found among different skin types. Conclusion： Age, solar-exposure time, Fitzpatrick type and skin type are the associated forces in promoting skin aging, and emotional factor seems to be another independent risk factor. The age of 49 years and 2 h/d of solar-exposure time seem to be the turning points responsible for dramatic changes of cutaneous appearance in the process of skin aging in Southeast China.
文摘Background:Oxidative stress is a significant factor in skin aging and pigmentation,which can be precipitated by various circumstances.Antioxidants and tyrosinase inhibitors,such as carotenoids,yeast extract(glutathione),sodium hyaluronate,astaxanthin,and niacin,can individually protect the skin against aging through distinct mechanisms.These mechanisms potentially enhance the skin barrier and improve signs of aging and pigmentation.However,the synergistic effects of these compounds,as found in a golden tomato extract formulation,have been scarcely explored.Objective:To evaluate the effects of an orally administered formulation on the skin aging and pigmentation.Material and Methods:In this study,a randomized,double-blind,parallel-controlled trial was conducted,utilizing the WONDERLAB?Tomato Niacinamide beverage.Out of all participants,62 volunteers completed the experiment and were included in the statistical analysis.Results:The results indicated that after eight weeks of consuming the research product,there were no significant changes in the skin indicators within the placebo group.In contrast,the treatment group receiving the sample formulation exhibited a 35.63%increase in stratum corneum hydration and a 29.39%reduction in transepidermal water loss(TEWL),suggesting enhanced skin hydration.Visual assessments revealed improvements in skin color and gloss index by 15.03%and 11.41%,respectively,in the treatment group.Furthermore,the skin gloss and individual typology angle(ITA)value increased by 18.59%and 6.36%,respectively,leading to a lighter skin tone.Significant enhancements were also observed in skin pigmentation,color uniformity,and redness.After eight weeks of intervention with the sample,blood levels of superoxide dismutase(SOD)and glutathione peroxidase(GPx)increased,while malondialdehyde(MDA)levels decreased.Conclusion:These findings confirm that continuous intake of the tomato extract formulation over eight weeks effectively improved the volunteers'skin whitening and hydration,and visibly brightened skin tone through an antioxidant mechanism.
文摘The“gut-skin”axis has been proved and is considered as a novel therapy for the prevention of skin aging.The antioxidant efficacy of oligomannonic acid(MAOS)makes it an intriguing target for use to improve skin aging.The present study further explored whereby MAOS-mediated gut-skin axis balance prevented skin aging in mice.The data indicated the skin aging phenotypes,oxidative stress,skin mitochondrial dysfunction,and intestinal dysbiosis(especially the butyrate and HIF-1a levels decreased)in aging mice.Similarly,fecal microbiota transplantation(FMT)from aging mice rebuild the aging-like phenotypes.Further,we demonstrated MAOS-mediated colonic butyrate-HIF-1a axis homeostasis promoted the entry of butyrate into the skin,upregulated mitophagy level and ultimately improving skin aging via HDAC3/PHD/HIF-1a/mitophagy loop in skin of mice.Overall,our study offered a better insights of the effectiveness of alginate oligosaccharides(AOS),promised to become a personalized targeted therapeutic agents,on gut-skin axis disorder inducing skin aging.
基金the Science and Technology Action Plan for Prevention and Treatment of Major Diseases sponsored by the National Health and Family Planning Commission of the People’s Republic of China(Grant No.2017ZX-01E-002)Anhui Institute of Translational Medicine(ZHYX2020A005).
文摘The skin is the largest organ of the human body,and its aging is visible to the naked eye.The aging rate of men and women is slightly different.This study compared the protein expression of skin samples on the curved forearms of 11 healthy women and 9 healthy men.Quantitative proteomics analysis found that the expression of epidermal proteins in men and women of the same age group was different.Compared with female skin,in male skin,20 proteins were upregulated,and 7 proteins were downregulated.These data suggest that men and women have differences in the speed of skin aging.For the first time in this experiment,a non-invasive mass spectrometer was used to detect 27 different-related epidermal proteins between men and women.Compared with women,among the 20 epidermal proteins upregulated in men,their functions can be classified into antioxidants,epidermal lipid metabolism,signal transduction,membrane transport,and cell biological processes;the 7 downregulated proteins are involved in a variety of biological processes and inflammatory reactions.Our experiments have discovered epidermal proteins related to the differences between men and women,enriching the library of epidermal differential proteins between men and women and enriching the mechanism of skin aging between men and women from the perspective of epidermal differential proteins.
文摘Background: Wrinkles, pigmented spots, and roughness are representative parameters reflecting facial skin aging. Sulci cutis connecting to follicular orifices frequently form perifollicular cracks, which join together adjacent sulci. This follicle-sulcus-crack complex (FSCC) is exacerbated by dehydration. Purpose: Although dehydration is associated with facial skin aging, FSCC’s implications in facial skin aging remain unresolved. Method: We measured facial skin parameters in 1999 and 2010 in 108 Japanese women, and in 2006 and 2016 in 182 Chinese women. We addressed whether expansion of FSCC is associated with other skin aging parameters. We also examined whether skin moisturizer can reverse the expanded FSCC. Results: In both clinical studies, FSCC severity as well as wrinkles, spots, and roughness significantly deteriorated with age. Notably, FSCC significantly increased as early as in subjects in their 20s, whereas wrinkles, spots, and roughness became conspicuous in those in their 40s or older. Moreover, subjects with more severe FSCC in the initial measurement exhibited significantly worse wrinkles, spots, and roughness in the second measurement a decade later. Dehydration was significantly correlated with expanded FSCC. Furthermore, FSCC expansion was reversed after appropriate moisturization by daily application of Galactomyces ferment filtrate (GFF)-containing skincare product (SK-II Skin Power Cream) for 4 weeks in 37 women. Conclusion: The expanded FSCC is likely to be an early indicator of facial skin aging. Appropriate moisturization may reduce FSCC formation and decelerate facial skin aging.
文摘Background: Aged skin exhibits visual alterations such as wrinkles, rough texture, pore dilation, and dull skin tone, as well as physiological aging, namely, decreased hydration and increased transepidermal water loss (TEWL). Recent advances in coherence tomography have also revealed that skin aging affects in vivo epidermal keratinocyte architecture. However, the interconnectivity between spatial architectural aging and visual/physiological aging parameters remains largely unknown. Purpose: To elucidate whether the tomographic keratinocyte architectural aging is correlated with visual and physiological skin aging parameters and to quantitatively evaluate the improvements of the architectural, visual, and physiological aging parameters by the daily treatment of the skin care formula containing Galactomyces Ferment Filtrate (GFF, 8X Pitera<sup>TM</sup>). Method: We measured the in vivo keratinocyte cellular architecture with two-photon stereoscopic tomography obtaining by-layer epidermal section images in 78 Asian females of various ages. Visual aging parameters were analyzed using a portable image capture system. Hydration and TEWL were also assessed. The anti-aging effects of GFF-containing skin moisturizer (SK-II LXP Cream<sup>TM</sup>) were also examined in two studies after twice-daily application for 2 (N = 35) and 4 (N = 32) weeks. Results: As for the keratinocyte cellular architecture, skin aging was significantly associated with decreased cell density and increased cell uniformity. These architectural aging parameters were significantly correlated with visual and physiological aging parameters, namely, rough texture, wrinkles, pore dilation, dull skin tone, dehydration, and increased TEWL. The strong interconnectivity allowed us to develop formulae to estimate the keratinocyte architecture from visual aging parameters. Moreover, twice-daily application of SK-II significantly improved the keratinocyte architecture associated with multiple skin aging visual and physiological parameters. Conclusion: Skin aging is a process involving mutual interconnections among epidermal keratinocyte cellular architecture, visual, and physiological parameters. The GFF-containing moisturizer SK-II effectively improves spatial architecture of keratinocytes in epidermis and these evaluated skin aging parameters in a new trajectory over the course of treatment. .
文摘This paper briefly introduce the test method for human evaluation of cosmetics to delay skin aging,and provide reference for the test of relevant indexes.By formulating a reasonable test plan and choosing appropriate equipment to test the improvement of the skin aging characteristics before and after the product is used,it can provide a scientific basis for claiming the effect of delaying skin aging.
文摘Skin aging is an unavoidable aspect of human life.Premature skin aging can result from poor care,environmental pollutants,and ultraviolet radiation exposure.Wrinkles,lines,spots,uneven skin tone,and pigmentation are often indicators of skin aging.One cannot avoid aging but cosmetics and pharmaceutical approaches can minimize and delay the damage.Topical applications of biocompatible and biodegradable vehicles have been explored for delivering anti-aging compounds.Lecithin organogel(LO)is an effective vehicle for topical delivery of many bioactive agents used in aging treatment.Lecithin is cell component isolated from soya beans or eggs and purified to show excellent gelation in non-polar solvents when combined with water.LO can form a heat-stable,resistant to microbial growth,visco-elastic,optically transparent,and non-birefringent micellar system.It serves as an organic medium to enhance dermal permeation of poorly permeable drugs by effectively partitioning into the skin.Its ability to dissolve in hydrophilic as well as in lipophilic drugs makes it a dynamic vehicle,which can be explored as a carrier for anti-aging agents.
基金the National Natural Science Foundation of China(Grant No.31971109)Shanghai Key Laboratory of Cell Engineering(Grant No.14DZ2272300)Peak Disciplines(Type IV)of Institutions of Higher Learning in Shanghai.The figures were created with BioRender.com.
文摘Owing to its constant exposure to the external environment and various stimuli,skin ranks among the organs most vulnerable to manifestations of aging.Preventing and delaying skin aging has become one of the prominent research subjects in recent years.Mesenchymal stem celis(MsCs)are multipotent stem cells derived from mesoderm with high self-renewal ability and multilineage differentiation potential.MSC-derived extracellular vesicles(MSC-EVs)are nanoscale biological vesicles that facilitate intercellular communication and regulate biological behavior.Recent studies have shown that MSC-EVs have potential applications in anti-aging therapy due to their anti-inflammatory,anti-oxidative stress,and wound healing promoting abilities.This review presents the latest progress of MSC-EVs in delaying skin aging.It mainly includes the MSC-EVs promoting the proliferation and migration of keratinocytes and fibroblasts,reducing the expression of matrix metalloproteinases,resisting oxidative stress,and regulating inflammation.We then briefly discuss the recently discovered treatment methods of MSc-EVs in the field of skin anti-aging.Moreover,the advantages and limitations of EV-based treatments are also presented.
基金supported by an NSFC grant (No.31470423) and a special fund for natural resources affairs (2019KJCX027) from Forest Administration of Guangdong Province,China.
文摘Air pollution adversely affects skin,leading to skin inflammation and premature skin aging.Plant derived antioxidant compounds have been considered to be promising in discovery of effective agents for the protection of skin from the damage by air pollutants.Our previous studies demonstrated that Averrhoa carambola fruit(known as star fruit)is rich in flavonoid C-glycosides with unique structures and potent antioxidant activity.Thus,the star fruit extract(SFE)and main flavonoid C-glycoside components,carambolasides I,J,and P(1-3),carambolaflavone B(4),and isovitexin 2″-O-α-l-rhamnoside(5),were investigated for the activity against air pollutant stress in human epidermis.As a result,SFE and compounds 1-5 exhibited significant inhibitory activity against protein carbonylation in oxidative-stressed stratum corneum with the best activity being shown by compound 3.SFE and compounds 2-5 were also active against engine exhaust-induced protein carbonylation in stratum corneum.When further evaluated,SFE and com-pound 3 significantly inhibited gene expression of the key inflammation mediators IL-1αand COX-2 in PM-stressed keratinocytes.The results indicated that SFE and the flavonoid C-glycosides are potentially effective against air pollutant-induced skin inflammation and premature aging.
文摘Objective： To observe the effect of acupuncture on skin aging of senile rats and discuss the mechanism of acupuncture in treating skin aging. Methods： The 18-month-old senile Wistar rats were randomly divided into three groups, a blank group, a surrounding needling group, a surrounding needling plus Zusanli （ST 36） group, comparing with the 8-month-old young rats. The tests included the content of dissoluble hydroxyproline （Hyp） and the activity of superoxide dismutase （SOD） in the skin of rats＇ abdomen. The transmission electron microscope was used for the morphological observation of the fibroblasts and the arrangement of the collagen fiber. Results： The dermal organelles in the fibroblasts of the rats decreased with the cellular structures. Meanwhile, the spaces among the collagen fibers extended and the aging bridging increased. The surrounding needling improved the activity of the fibroblasts. The dissoluble Hyp content in the surrounding needling group was much higher than the rats in the blank group, but the SOD activity didn＇t change significantly. However, the surrounding needling plus Zusanli （ST 36） enhanced the SOD activity. Conclusion： Acupuncture may improve the activity of the fibroblasts in the skin to increase the dissoluble collagen content, resulting in the improvement of an aged appearance. Surrounding needling plus needling Zusanli （ST 36） could enhance the SOD activity.
基金supported by the National Natural Science Foundation of China[grant number:82273555]the Beijing Municipal Nature Science Foundation[grant number:L234068].
文摘Skin aging and wound healing have always been significant topics in the skin field.Hyaluronic acid(HA),a natural glycosaminoglycan(GAG)presenting in the extracellular matrix(ECM),is extensively applied in the fields of anti-aging therapeutics and wound repair due to its unique physicochemical and biological properties.HA is indispensable in the regulation of diverse biological processes such as cellular proliferation,differentiation,migration,signaling,inflammatory responses,angiogenesis,and tissue regeneration.Consequently,HA-based formulations,including creams,gels,serums,and dermal fillers,are instrumental in combating wrinkles,enhancing skin hydration,correcting tear trough deformity,and restoring facial volume.Additionally,the inherent biocompatibility and high biodegradability of HA facilitates its chemical modification,rendering it an ideal material for wound healing and tissue regeneration.This review summarizes the recent advances and applications of the molecular mechanisms of HA and its derivatives in the field of anti-aging and wound healing;and discusses the prospects and challenges of HA-based compounds in tissue repair and skin regeneration.
文摘<strong>Background:</strong> Young and intact skin is highly responsive. As skin ages, its responsiveness decreases. Production of structural components of the skin slows down and signs of skin aging appear. External influences like UV radiation and pollution contribute to signs of skin aging. They lead to an increased formation of so-called free radicals. Bioactive antioxidant compounds, such as polyphenols, have beneficial effects on skin health by free radical scavenging. The purpose of this study was to evaluate the topical effects of new and highly concentrated resveratrol containing emulsion (Medskin Solutions Dr. Suwelack AG, 2% trans-resveratrol) on age-related alterations to the skin. <strong>Methods:</strong> A clinical observation study was performed on 20 subjects to observe the effects of a 2% resveratrol emulsion on typical signs of skin aging. Product was applied once daily for a period of 8 weeks in combination with a standardized treatment. Tested parameters were skin elasticity, skin barrier function, skin smoothness, skin thickness and density. <strong>Results:</strong> After 8 weeks of treatment, skin pH value is significantly lowered and skin barrier function improved. The value of skin elasticity (+5.3%), and skin density (+ 10.7%) had increased, while skin roughness (-6.4%) and skin dispensability (-45.9%) had diminished. Intensity of skin redness had decreased. Furthermore, expert and participant evaluations show satisfaction, especially with regard to skin smoothness and skin moisture. Conclusion: The resveratrol emulsion shows positive outcomes by supporting reduction of skin wrinkling and increasing skin firmness, as well as reducing skin redness, and is therefore an all-round anti-aging treatment.
文摘Anti-aging research has become a popular scientific field with the increasing prominence of population aging.Rare ginsenoside Compound K(CK)has attracted widespread attention as an emerging anti-aging active ingredient.The anti-aging effect of ginsenosides is considered to be one of the important roles of ginsenosides,and Compound K,as the main deglycosylated metabolite of ginsenosides,has a comprehensive anti-aging effect as a highly active ingredient obtained by transformation under the action of microbiota.Recent studies have shown that ginsenosides have anti-photo-oxidation,anti-skin aging,free radical scavenging and immunostimulatory effects,which can effectively prevent skin photoaging.With the progress of modern natural medicine extraction technology and the deepening of the research on the anti-skin aging of ginsenosides'high active ingredients,it will promote the development and application of natural product protective skin photoaging preparations.The rare ginsenoside Compound K plays an important role in the improvement of skin health and anti-aging,which is mainly realized by increasing the activity of antioxidant enzymes,inducing the expression of related genes,reducing the content of oxidative damage substances,regulating the immune system,and influencing the expression of cell-cycle regulators and aging genes.A more comprehensive and in-depth study of the molecular mechanism of the anti-aging effect of rare ginsenoside Compound K will be one of the focuses of future research.
文摘Background: As the population of people aged 65 and older increases, greater attention is being paid to issues surrounding skin aging. This study was conducted to evaluate the efficacy of an oral unhydrolyzed eggshell membrane (ESM) ingredient (branded Ovolux™) versus placebo on both objective and subjective markers of skin aging in middle-aged and senior adults, and also to assess the possible benefits of Ovolux™ on the health of hair and fingernails. Methods: Sixty-three healthy, men and women (aged 35 - 66) were randomly assigned to receive either oral ESM 300 mg (n = 32) or placebo (n = 31) once daily for 12 weeks. The primary endpoint was any statistically significant improvement in skin elasticity in the anterior region of the temporal fossa of the face versus placebo evaluated at 12 weeks of treatment based upon measurements taken with the Cutometer® MPA 580 multi-probe system. Secondary endpoints were improvement in skin firmness, viscoelasticity, hydration, or trans-epidermal water loss versus placebo. Additional secondary endpoints were any improvement in skin, hair, or fingernail subjective parameters versus placebo as determined via Patient’s Assessments. Results: Supplementation with Ovolux™ ESM produced a significant absolute treatment effect (TEabs) versus placebo after both six weeks (TEabs +8.4%, p = 0.019) and twelve weeks of treatment (TEabs +12.2%, p = 0.002) for the primary outcome measure total elasticity (R2). There were also significant treatment responses versus placebo for the objective measures of firmness (R0), net elasticity (R5), and viscoelasticity (R6) at both timepoints (R0: Week 6, −23.9%;Week 12, −16.8%) (R5: Week 6, +9.5%;Week 12, +10.6%) (R6: Week 6, −17.9%;Week 12, −17.7%), as well as for skin hydration versus placebo at both 6 weeks (+8.3%) and 12 weeks (+8.6%) of treatment. There were also significant improvements in the ESM treatment group versus placebo in the subjective measures of skin appearance, skin texture, overall skin health, hair texture, and overall hair health as determined by patient assessments. Conclusions: Ovolux™ provided significant broad-based improvements in critical biomechanical skin properties versus placebo, including elasticity, firmness, and viscoelasticity. These effects, along with improvement in skin hydration, were observed as early as 6 weeks and continued through the end of the 12-week study. Treatment with ESM was reported to be well-tolerated. Ovolux™ ESM appears to be a natural solution to help skin to resist the epidermal thickening, the dermal thinning, and the skin laxity and elastosis found in intrinsically aging skin. The Clinical Trial Registration number is: NCT06148337.
文摘Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR.
文摘Each biological system possesses a widely unrecognized buffer system to maintain acid-base balance to a specific pH. The skin pH is crucial for physiological skin function. In aged or diseased skin, pH increase is observed and may negatively affect skin health. Skin care products with a pH that is slightly more acidic than the average normal skin pH and have an adequate buffering capacity, are considered beneficial for the skin. However, the buffer capacity of these products also plays an important role. In the present study, a possible normalization or acidification of skin surface pH and influence on skin hydration and skin barrier function was assessed via application of buffered skin care products that are formulated with pH ≤ 4.5. 48 subjects aged above 50 were treated with three different skin care products (Vitamin C Spheres, Collagen Spray, and Collagen Mask) and skin surface pH, skin hydration and barrier integrity were assessed before treatment start and after 4 weeks. The results show that after 4-week treatment with Vitamin C, the skin pH is acidified. Treatment with Collagen Spray and Collagen Mask showed maintenance of a physiological skin pH. Subgroup analysis of subjects that had a higher than average skin pH at study start demonstrated that all three tested skin care products were able to acidify the skin surface. In addition, skin hydration was also increased for two of the three tested products, whereas skin barrier is not significantly changed. This demonstrates that buffered skin care products formulated to a pH ≤ 4.5 are able to acidify and maintain physiological skin pH and may contribute to a physiological skin function.
基金supported in part by grants from the National Key Research and Development Program of China(2021YFD1600204 and 2023YFF1104500)the 111 project from the Ministry of Education of the People’s Republic of China 577(B18053).
文摘Skin aging is an increasingly prominent topic in the context of healthy aging.During the aging process,the skin’s barrier function diminishes,its water content decreases,wrinkles begin to form,and changes occur in the gut microbiota composition.However,the relationship between gut microbiota and skin aging remains unclear.In this study,we explored skin rejuvenation in aged mice through fecal microbiota trans-plantation(FMT)using feces from young mice.The results demonstrated enhanced water retention,thick-ened stratum corneum,increased collagen content,and improved epithelial cell differentiation in aged mice following FMT.Notably,FMT particularly increased the abundance of Lactobacillus and Lactococcus in aged mice,which were nearly undetectable in untreated aged mice.Non-targeted and targeted meta-bolomics analyses indicated that FMT significantly elevated levels of tryptophan(Trp)and its microbiota metabolites(e.g.,indole-3-lactic acid(ILA))in the feces and serum of aged mice.Both Trp and ILA appeared to rejuvenate aged skin by activating the aryl hydrocarbon receptor(AhR)to promote epidermal cell dif-ferentiation.In conclusion,FMT from young mice rejuvenated aged skin via Trp-metabolizing bacteria(Lactobacillus and Lactococcus)and Trp-derived metabolites,suggesting that interventions targeting Trp metabolites may effectively improve skin aging.
