The Lower Cambrian Longwangmiao Fm carbonatite gas reservoirs in the Leshan-Longnüsi Paleouplift in the Sichuan Basin feature strong heterogeneity,well-developed fractures and caverns,and a high content of H_(2)S...The Lower Cambrian Longwangmiao Fm carbonatite gas reservoirs in the Leshan-Longnüsi Paleouplift in the Sichuan Basin feature strong heterogeneity,well-developed fractures and caverns,and a high content of H_(2)S,so these reservoirs are prone to reservoir damages caused by the invasion of drilling fluid or the improper well completion,so to minimize the reservoir skin factor is key to achieving high yield of oil and gas in this study area.Therefore,based on the geological characteristics of the Longwangmiao reservoirs,the binomial productivity equation was applied to demonstrate the possibility and scientificity of minimizing the skin factor.According to the current status of reservoir stimulation,the overall skin factors of reservoir damage caused by drilling fluid invasion,improper drilling and completion modes etc were analyzed,which shows there is still potential for skin factor reduction.Analysis of reservoir damage factors indicates that the main skin factor of Longwangmiao Fm reservoirs consists of that caused by drilling fluid and by improper completion modes.Along with the minimization of skin factor caused by drilling and improper completion,a fracture-network acidizing process to achieve“non-radial&network-fracture”plug-removal by making good use of natural fractures was proposed according to the characteristics of Longwangmiao Fm carbonatite reservoirs.展开更多
AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed...AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed and skin grafted,the rhEGF was injected under the skin graft.80 cases without injection of rhEGF were made as contrast.Ten days later,the area of survived skin was measured and the livability of skin was calculated. RESULTS: The skin livability of cases with in jection of rhEGF was (90.67±10.02)% and the skin livability of contrast cases was(76.85±8.35)%. There axisted evident differences between them(P< 0.01).CO NCLUSION:The rhEGF was an effective method for increasing livability of skin gra ft.展开更多
Formation damage evaluation is an important component in well test analysis and for- mation evaluation. Formation damage of one-layer reservoir has been studied by decomposing the total skin factor and estimating the ...Formation damage evaluation is an important component in well test analysis and for- mation evaluation. Formation damage of one-layer reservoir has been studied by decomposing the total skin factor and estimating the mud pollution depth. And the well test analyses for multilayered reservoirs were reported, but relation between the total skin factor and individual layer skin factor has not been considered. The relation is devel-oped in this note with well test analysis combining numerical simulation, which aids properly evaluating formation and making stimulating decision.展开更多
Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced ...Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced by such antibodies negatively impact on the quality of life of patients and decreases drug compliance during treatment. If we can predict the high-risk group susceptible to severe skin toxicity before treatment, we can undertake the early management of any arising skin disorders and formulate a more accurate prognosis for anti-EGFR antibody treatment. Previous studies have identified molecular markers of skin toxicity induced by anti-EGFR antibody, such as EGFR polymorphisms, the expression of inflammatory chemokines and serum levels of EGFR ligands. A clinical trial was undertaken involving the escalation of cetuximab doses, guided by the grade of skin toxicity observed, such as no or low-grade, in metastatic colorectal cancer(the EVEREST study). The dose escalation of cetuximab was confirmed by a safety profile and had the tendency to achieve a higher response rate in KRAS wild-type patients. A large, prospective randomized trial is now ongoing(EVEREST 2) and the results of this trial may contribute to personalized medicine in KRAS wild-type colorectal cancer patients.展开更多
Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and ...Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and allografts on burnt rats were quantitatively analysed. Results (1) mRNA expressions ofall the four growth factors mentioned above were found both in allografts and autografts, the expressions beinghigher in autografts than allografts; (2) In autografts, TGFα expression began to rise from second post transplantday (D2), reaching a peak on D18,. TGFβ1 expression gained a high level on D2 and had itS peak on D18; PDGFexpression had its peak on D4, rebounding on D25; bFGF expression, exceedingly high on D2, dropped andrebounded in the same manner as in PDGF; (3) In the early stage of the wound healing, mRNA expression of thefour growth factors was lower in allografts, rising up in varying degrees thereafter. Conclusion (1) Graftsurvival took place both in autografts and allografts, with the former pederming better than the latter; (2) Thecombining action of growth factors that reached the affected area through autosecretion and bysecretion probablypromoted the healing of the burn wound, following signal transmission channels; (3) Rejection, which occurred inallogralts, was responsible for the decreasing viability of allografts and their replacement by the host skin.展开更多
This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC...This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.展开更多
Fabric-skin adhesion was objectively described by the indices of the maximum adhesion force F_(max) , the maximum separation distance L_(max) ,and adhesion work W as well as the adhesion force-separation distance curv...Fabric-skin adhesion was objectively described by the indices of the maximum adhesion force F_(max) , the maximum separation distance L_(max) ,and adhesion work W as well as the adhesion force-separation distance curve. Firstly,gray correlation analysis method was adopted to investigate the correlation levels between adhesion indices,and secondly the relative importance of fabric structural parameters to fabric-skin adhesion,as well as the correlation levels between skin adhesion, water absorption, and wicking properties of the fabric. The results prove that W exhibits clear correlations with both F_(max) and L_(max) , yet the relevance between F_(max) and L_(max) is weak. Fabric adhesion indices are most associated with fabric mass and least with fabric thickness,whereas fabric wicking and water absorption present closest correlation with fabric thickness. Therefore, it is concluded that the relevance between fabric wicking, water absorption, and skin-adhesion properties are rather comprehensive than straight.展开更多
目的系统评价成人危重症患者急性皮肤衰竭(ASF)的影响因素。方法计算机检索Web of Science、PubMed、维普数据库、万方知识数据服务平台、CNKI数据库(检索时限均自建库至2025年11月1日)成人危重症患者ASF影响因素的随机对照试验和观察...目的系统评价成人危重症患者急性皮肤衰竭(ASF)的影响因素。方法计算机检索Web of Science、PubMed、维普数据库、万方知识数据服务平台、CNKI数据库(检索时限均自建库至2025年11月1日)成人危重症患者ASF影响因素的随机对照试验和观察性研究。本研究为定量综述,由2名研究者独立进行文献筛选、数据提取以及对纳入研究的偏倚风险评价后进行。结果共纳入6项研究,包括研究对象196804例。Meta分析结果显示,机械通气(对数OR 1.975,95%CI 0.914~3.037,P<0.05)与血管活性药物使用(对数OR 1.606,95%CI 0.279~2.932,P<0.05)是成人危重症患者发生ASF的显著危险因素。然而,肾衰竭与ASF关联的Meta分析结果未达到统计学显著性(对数OR 0.740,95%CI-0.033~1.513,P>0.05),且异质性较高(I^(2)=85.09%),提示结果稳定性受潜在混杂因素影响。结论应根据影响因素制定预防成人危重症患者ASF的干预措施。受纳入的研究在数量上的有限性与方法学质量上的异质性限制,上述结论尚需开展更多大样本、多中心、设计严谨的前瞻性研究加以证实。展开更多
基金National science and technology major project“Complex Carbonate Gas Reservoir Development Technology Research”(No.2011ZX05015-003-005).
文摘The Lower Cambrian Longwangmiao Fm carbonatite gas reservoirs in the Leshan-Longnüsi Paleouplift in the Sichuan Basin feature strong heterogeneity,well-developed fractures and caverns,and a high content of H_(2)S,so these reservoirs are prone to reservoir damages caused by the invasion of drilling fluid or the improper well completion,so to minimize the reservoir skin factor is key to achieving high yield of oil and gas in this study area.Therefore,based on the geological characteristics of the Longwangmiao reservoirs,the binomial productivity equation was applied to demonstrate the possibility and scientificity of minimizing the skin factor.According to the current status of reservoir stimulation,the overall skin factors of reservoir damage caused by drilling fluid invasion,improper drilling and completion modes etc were analyzed,which shows there is still potential for skin factor reduction.Analysis of reservoir damage factors indicates that the main skin factor of Longwangmiao Fm reservoirs consists of that caused by drilling fluid and by improper completion modes.Along with the minimization of skin factor caused by drilling and improper completion,a fracture-network acidizing process to achieve“non-radial&network-fracture”plug-removal by making good use of natural fractures was proposed according to the characteristics of Longwangmiao Fm carbonatite reservoirs.
文摘AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed and skin grafted,the rhEGF was injected under the skin graft.80 cases without injection of rhEGF were made as contrast.Ten days later,the area of survived skin was measured and the livability of skin was calculated. RESULTS: The skin livability of cases with in jection of rhEGF was (90.67±10.02)% and the skin livability of contrast cases was(76.85±8.35)%. There axisted evident differences between them(P< 0.01).CO NCLUSION:The rhEGF was an effective method for increasing livability of skin gra ft.
文摘Formation damage evaluation is an important component in well test analysis and for- mation evaluation. Formation damage of one-layer reservoir has been studied by decomposing the total skin factor and estimating the mud pollution depth. And the well test analyses for multilayered reservoirs were reported, but relation between the total skin factor and individual layer skin factor has not been considered. The relation is devel-oped in this note with well test analysis combining numerical simulation, which aids properly evaluating formation and making stimulating decision.
文摘Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced by such antibodies negatively impact on the quality of life of patients and decreases drug compliance during treatment. If we can predict the high-risk group susceptible to severe skin toxicity before treatment, we can undertake the early management of any arising skin disorders and formulate a more accurate prognosis for anti-EGFR antibody treatment. Previous studies have identified molecular markers of skin toxicity induced by anti-EGFR antibody, such as EGFR polymorphisms, the expression of inflammatory chemokines and serum levels of EGFR ligands. A clinical trial was undertaken involving the escalation of cetuximab doses, guided by the grade of skin toxicity observed, such as no or low-grade, in metastatic colorectal cancer(the EVEREST study). The dose escalation of cetuximab was confirmed by a safety profile and had the tendency to achieve a higher response rate in KRAS wild-type patients. A large, prospective randomized trial is now ongoing(EVEREST 2) and the results of this trial may contribute to personalized medicine in KRAS wild-type colorectal cancer patients.
文摘Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and allografts on burnt rats were quantitatively analysed. Results (1) mRNA expressions ofall the four growth factors mentioned above were found both in allografts and autografts, the expressions beinghigher in autografts than allografts; (2) In autografts, TGFα expression began to rise from second post transplantday (D2), reaching a peak on D18,. TGFβ1 expression gained a high level on D2 and had itS peak on D18; PDGFexpression had its peak on D4, rebounding on D25; bFGF expression, exceedingly high on D2, dropped andrebounded in the same manner as in PDGF; (3) In the early stage of the wound healing, mRNA expression of thefour growth factors was lower in allografts, rising up in varying degrees thereafter. Conclusion (1) Graftsurvival took place both in autografts and allografts, with the former pederming better than the latter; (2) Thecombining action of growth factors that reached the affected area through autosecretion and bysecretion probablypromoted the healing of the burn wound, following signal transmission channels; (3) Rejection, which occurred inallogralts, was responsible for the decreasing viability of allografts and their replacement by the host skin.
文摘This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.
基金National Natural Science Foundation of China(No.60904056)Shanghai Municipal Natural Science Foundation,China(No.14ZR1401000)+2 种基金Science and Technology Project of Administration of Quality and Technology Supervision of Guangzhou Municipality,China(No.2014KJ29)Science and Technology Guidance Project of China National Textile and Apparel Council,China(No.2015066)the Fundamental Research Funds for the Central Universities,China
文摘Fabric-skin adhesion was objectively described by the indices of the maximum adhesion force F_(max) , the maximum separation distance L_(max) ,and adhesion work W as well as the adhesion force-separation distance curve. Firstly,gray correlation analysis method was adopted to investigate the correlation levels between adhesion indices,and secondly the relative importance of fabric structural parameters to fabric-skin adhesion,as well as the correlation levels between skin adhesion, water absorption, and wicking properties of the fabric. The results prove that W exhibits clear correlations with both F_(max) and L_(max) , yet the relevance between F_(max) and L_(max) is weak. Fabric adhesion indices are most associated with fabric mass and least with fabric thickness,whereas fabric wicking and water absorption present closest correlation with fabric thickness. Therefore, it is concluded that the relevance between fabric wicking, water absorption, and skin-adhesion properties are rather comprehensive than straight.
