Esthetic treatments can induce swelling and bruises. Thus, a treatment that would prevent or hasten the resolution of bruising should be very useful. Generally, the regression of bruising was conducted with patients o...Esthetic treatments can induce swelling and bruises. Thus, a treatment that would prevent or hasten the resolution of bruising should be very useful. Generally, the regression of bruising was conducted with patients or animals models. So we decided firstly to develop an ex vivo model in order to test antibruising properties of topical formulations and secondly to evaluate a curative effect of a cream (mixture of arnica extract and apigenin) in comparison with a positive control (vulnerary cream) and also to estimate the preventive interest of this cream. The results showed that the injection of 25 μl of blood into the dermis of skin fragments was sufficient to create a model of induced-bruise. The duration of 24 hours was chosen to compare the effects of actives on the decrease in the size of the bruise. Joint effects of a pretreatment and a treatment of a mixture of arnica extract and apigenin decreased significantly the area of bruising compared to the treatment group, the control group and the positive control group. Many topical products claim to improve bruising on their package label. Our model can demonstrate their efficacy and determinate the best topical antibruising formulation. The mechanism involved in anti-inflammatory activity of active compounds of topical formulations is often not fully understood. Our blood-induced model may bring some responses through the study of mediators of the inflammation.展开更多
Since the introduction of electroporation a decade ago,the chick embryo has evolved into a powerful system for studying gene regulation and function during development.Although this is a straightforward method for emb...Since the introduction of electroporation a decade ago,the chick embryo has evolved into a powerful system for studying gene regulation and function during development.Although this is a straightforward method for embryos,skin(especially lamellar tissue)present special challenges.Here we describe a protocol for electroporation of expression vectors into the chick skin explant,with precise spatial and temporal control.We have improved the design of the electroporation chamber,which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate.We give the electroporation parameters of chicken embryo skin,which can effectively improve the electroporation efficiency.This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin.展开更多
文摘Esthetic treatments can induce swelling and bruises. Thus, a treatment that would prevent or hasten the resolution of bruising should be very useful. Generally, the regression of bruising was conducted with patients or animals models. So we decided firstly to develop an ex vivo model in order to test antibruising properties of topical formulations and secondly to evaluate a curative effect of a cream (mixture of arnica extract and apigenin) in comparison with a positive control (vulnerary cream) and also to estimate the preventive interest of this cream. The results showed that the injection of 25 μl of blood into the dermis of skin fragments was sufficient to create a model of induced-bruise. The duration of 24 hours was chosen to compare the effects of actives on the decrease in the size of the bruise. Joint effects of a pretreatment and a treatment of a mixture of arnica extract and apigenin decreased significantly the area of bruising compared to the treatment group, the control group and the positive control group. Many topical products claim to improve bruising on their package label. Our model can demonstrate their efficacy and determinate the best topical antibruising formulation. The mechanism involved in anti-inflammatory activity of active compounds of topical formulations is often not fully understood. Our blood-induced model may bring some responses through the study of mediators of the inflammation.
基金the National Natural Science Foundation of China(12222201,82273500,11972206,11902114,U20A20390,11827803,and 11902020)Fundamental Research Funds for the Central Universities(ZG140S1971)。
文摘Since the introduction of electroporation a decade ago,the chick embryo has evolved into a powerful system for studying gene regulation and function during development.Although this is a straightforward method for embryos,skin(especially lamellar tissue)present special challenges.Here we describe a protocol for electroporation of expression vectors into the chick skin explant,with precise spatial and temporal control.We have improved the design of the electroporation chamber,which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate.We give the electroporation parameters of chicken embryo skin,which can effectively improve the electroporation efficiency.This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin.
