the investigation was carried out on 10-year-old plantation of Fraxinus mandshurica in Mao抏r Mountain Experimental Station of Northeast Forest University. Tree height (H), diameter at breast height (D1.3) and the inc...the investigation was carried out on 10-year-old plantation of Fraxinus mandshurica in Mao抏r Mountain Experimental Station of Northeast Forest University. Tree height (H), diameter at breast height (D1.3) and the increment of tree height in 5 years (H5), the thickness of humus layer, as well as the soil moisture were measured for the plantation and the growth indexes (H, D1.3, H5) for different site conditions were analyzed. The results showed that main site factors influencing the growth of Fraxinus mandshurica were soil moisture, gradient and location of slope in order. The growth of Fraxinus mandshurica was better on the middle- or up-slope site than on the down-slope site. Soil moisture and late frost caused by terrain are the main reasons that limit the growth of Fraxinus mandshurica plantation.展开更多
Incidence of Armillaria infection was quantified based on site factors in New Zealand Pinus radiata plantations.A linear multiple regression model was derived to predict infection levels of Armillaria root rot.Factors...Incidence of Armillaria infection was quantified based on site factors in New Zealand Pinus radiata plantations.A linear multiple regression model was derived to predict infection levels of Armillaria root rot.Factors positivily associated with the infection were:previous vegetation（native bush,pine）;soil type（pumice）;landform （valley,gully,flat）and the interaction between them.This model could assist in management planning with regard to the predisposition of particular stand to Armillaria infection.Keywods:Armillaria root rot,Disease incidence,Site factors,Quantification,Pinus radiata.展开更多
Dahurian larch (Larix gmelinii Rupr.) Rupr is one of the dominant species in bothnatural and man-made forests in northeast China.Effects of site factors,including topography,soilproperties and nutrient elements(P,Ca,K...Dahurian larch (Larix gmelinii Rupr.) Rupr is one of the dominant species in bothnatural and man-made forests in northeast China.Effects of site factors,including topography,soilproperties and nutrient elements(P,Ca,K,Mg,Mn,Zn,Cu,Ag,Co and Ni)on the growth of larchwere analyzed in detail,qualitatively and quantitatively.The ordination technique was used to groupthe site factors and to classify sites for larch.Analysis indicated that height growth,diameter(DBH)and volume of the larch varied among site types and this will be of theoretical and practical signifi-canee in developing larch plantations.展开更多
Background: Cesarean sections have become increasingly prevalent in both developed and developing nations. Nevertheless, postoperative complications, including surgical site infections (SSIs), remain a substantial con...Background: Cesarean sections have become increasingly prevalent in both developed and developing nations. Nevertheless, postoperative complications, including surgical site infections (SSIs), remain a substantial concern that contributes to heightened morbidity and mortality. This study is designed to evaluate the frequency and key determining factors associated with surgical site infections following cesarean section procedures in a tertiary care hospital in Bangladesh. Materials and Methods: This observational cross-sectional study, conducted at the Gynecology Department of Dhaka Medical College Hospital, involved 100 patients aged 15 - 45 who had cesarean deliveries. Data was collected during hospitalization and post-discharge and analyzed to determine the prevalence and relationship between socio-demographic characteristics and surgical site infection. Results: Among the study participants, 14% developed surgical site infections following cesarean operations. More than half of these patients were under the age of 25, with a mean age of 24.45 ± 4.44 years. Surgical site infections were more prevalent in individuals over 30 years old (P-value Conclusion: Post-cesarean surgical site infections are notably prevalent among the participants in this study. Several risk factors have been identified, including age, body mass index (BMI), socioeconomic status, anemia, preterm delivery, personal hygiene practices, regular menstrual cycles, and adherence to antenatal check-ups. The implementation of an effective awareness program, coupled with updated antibiotic protocols, is crucial for significantly reducing the incidence of these infections.展开更多
Purpose: This paper aims to assess the incidences and risk factors for surgical site (ISO) infections in the general surgery department of the Koutiala District Hospital. Patients and Methods: This was a prospective a...Purpose: This paper aims to assess the incidences and risk factors for surgical site (ISO) infections in the general surgery department of the Koutiala District Hospital. Patients and Methods: This was a prospective and descriptive study from August 1, 2017 to October 31, 2020 involving all patients who underwent laparotomy in the general surgery department of the Koutiala District Hospital. Patients who were not operated on and who did not have a laparotomy were not included. Age, sex, frequency, patient history, National Nosocomial Infections Surveillance (NNIS) index, time to occur, bacteriological test results and course of infection were analyzed. Results: Fifty patients were registered. The average age was 34.2 ± 21.2 years old. Eleven patients were 60 years old or older. The hospital incidence rate was 4.3% and the community incidence rate was 6.1 cases per 100,000 population. The concept of smoking was noted in 15 patients. Patients were operated on in emergency 84% of cases. Peritonitis was the most common initial lesion with 32% of cases. Our patients had an NNIS index greater than 0 in 84% of cases. The time to develop infection from the surgical site was less than 3 days in 8 patients (16% of cases). Depending on the depth of the infection, it was superficial (cutaneous) in 39 patients (78%) and deep (subcutaneous and organic) in 11 patients (22%). Escherichia coli was the most common germ with 72% followed by staphylococcus aureus at 24%. We noted 22% morbidity. The median length of hospitalization was 9 ± 2.2 days. Conclusion: ISO is common in our service. Prevention and mastery require knowledge of risk factors.展开更多
Recent work analysing magnesium hydrogenation using Reflecting Electron Energy Loss Spectroscopy(REELS)and Density Function Theory(DFT)has indicated interfacial polarisation and interstitial hydrogen clustering influe...Recent work analysing magnesium hydrogenation using Reflecting Electron Energy Loss Spectroscopy(REELS)and Density Function Theory(DFT)has indicated interfacial polarisation and interstitial hydrogen clustering influence the reaction rate.The site availability model has been modified to include interstitial hydrogen clustering within the site availability factor and interface polarisation using interface treatment.The new model,SAM-CV-S,has demonstrated improved modelling of magnesium hydrogenation across wide operating conditions,such as temperatures from 330 to 400℃and pressures up to 40 bar.This wide applicability makes it a robust model that can be used to simulate bed performance in solid-state hydrogen stores.Thus,the site availability factor successfully combines interstitial hydrogen clustering with thermal resistance effects,which are known to strongly influence metal hydride reactor designs at scale.The next phase of the model is to incorporate a predictive hydrogen capacity method into the model.展开更多
Understanding the functional effects of genetic variants is crucial in modern genomics and genetics. Transcription factor binding sites （TFBSs） are one of the most important cis-regulatory elements. While multiple t...Understanding the functional effects of genetic variants is crucial in modern genomics and genetics. Transcription factor binding sites （TFBSs） are one of the most important cis-regulatory elements. While multiple tools have been developed to assess functional effects of genetic variants at TFBSs, they usually assume that each variant works in isolation and neglect the potential ＂interference＂ among multiple variants within the same TFBS. In this study, we presented COPE-TFBS （Context-Oriented Predictor for variant Effect on Transcription Factor Binding Site）, a novel method that considers sequence context to accurately predict variant effects on TFBSs. We systematically re-analyzed the sequencing data from both the 1000 Genomes Project and the Genotype-Tissue Expression （GTEx） Project via COPE-TFBS, and identified numbers of novel TFBSs, transformed TFBSs and discordantly annotated TFBSs resulting from multiple variants, further highlighting the necessity of sequence context in accurately annotating genetic variants.展开更多
The digital seismometers with high accuracy are installed at depths of -200 m, -100 m in borehole and on the surface in Dongchuan. By analysis of spectra ratio, the local events (with epicentral distances between 8.5 ...The digital seismometers with high accuracy are installed at depths of -200 m, -100 m in borehole and on the surface in Dongchuan. By analysis of spectra ratio, the local events (with epicentral distances between 8.5 km and 95 km, magnitudes ML = 1.3-5.1) recorded are used to calculate site magnification factor at different depths (-100 m and surface) in Dongchuan. Comparing results with that at the depth of -200 m, the site magnification factors at three components of N-S, E-W and U-D corresponding to the depth of -100 m at 8 frequencies (f = 0.75, 1.0, 1.5, 2, 3, 4, 6, and 12 Hz) are 1.0-1.49 when 0.75 Hz- [less-than or equal to] f [less-than or equal to] 2 Hz and 0.47-0.99 when 2 Hz less than or equal f less than or equal 12 Hz. On the surface, site magnification factors at three components are all greater than 1, i.e. between 1.6 and 7.63. The correlation coefficients of site magnification factors for S wave and coda at the depth of -100 m and on surface are 0.82 and 0.81 when 0.75 Hz [less-than or equal to] f [less-than or equal to] 2 Hz, 0.47 and 0.45 when 2 Hz less than or equal f less than or equal 12 Hz, respectively.展开更多
AIM To identify punitive transcriptional factor binding sites(TFBS) from regulatory single nucleotide polymorphisms(rS NPs) that are significantly associated with disease.METHODS The genome-wide association studies ha...AIM To identify punitive transcriptional factor binding sites(TFBS) from regulatory single nucleotide polymorphisms(rS NPs) that are significantly associated with disease.METHODS The genome-wide association studies have provided us with nearly 6500 disease or trait-predisposing SNPs where 93% are located within non-coding regions such as gene regulatory or intergenic areas of the genome. In the regulatory region of a gene, a SNP can change the DNA sequence of a transcriptional factor(TF) motif and in turn may affect the process of gene regulation. SNP changes that affect gene expression and impact gene regulatory sequences such as promoters, enhancers, and silencers are known as rS NPs. Computational tools can be used to identify unique punitive TFBS created by rS NPs that are associated with disease or sickness. Computational analysis was used to identify punitive TFBS generated by the alleles of these rS NPs.RESULTS r SNPs within nine genes that have been significantly associated with disease or sickness were used to illustrate the tremendous diversity of punitive unique TFBS that can be generated by their alleles. The genes studied are the adrenergic, beta, receptor kinase 1, the v-akt murine thymoma viral oncogene homolog 3, the activating transcription factor 3, the type 2 demodkinase gene, the endothetal Per-Arnt-Sim domain protein 1, the lysosomal acid lipase A, the signal Transducer and Activator of Transcription 4, the thromboxane A2 receptor and the vascular endothelial growth factor A. From this sampling of SNPs among the nine genes, there are 73 potential unique TFBS generated by the common alleles comparedto 124 generated by the minor alleles indicating the tremendous diversity of potential TFs that are capable of regulating these genes.CONCLUSION From the diversity of unique punitive binding sites for TFs, it was found that some TFs play a role in the disease or sickness being studied.展开更多
The risk to develop an infection after surgery depends on several factors. Those factors may be interdependent or not, depending on the nature of the surgery and the general condition of the patient. The aim of the pr...The risk to develop an infection after surgery depends on several factors. Those factors may be interdependent or not, depending on the nature of the surgery and the general condition of the patient. The aim of the present study was to identify the factors involved in the occurrence of the Surgical Site Infections (SSI) in the <strong><em>National referral general hospital </em></strong>of N’djamena (Chad). We first realized an interview in the emergency services and in the general surgery of the hospital. The information collected allowed us to draw up survey sheets. An investigation was then conducted on 152 patients who had surgery and were hospitalized during the study period. The frequency of the SSI was 33.6% (51/152). The main risk factors that we identified were the duration of hospitalization, the category of the hospitalization room, the urgent aspect of the surgical intervention, the patient’s nutritional status and the associated diseases with diabetes, which was present in 52.38% (11/21) of cases of associated pathologies. Analysis of the antibiotic prophylaxis administered to patients showed that ciprofloxacin was more effective in SSI prevention. Indeed, this antibiotic showed the fewest cases of infection with only 1.9% of patients having developed SSI. Our results show a very high frequency of SSI at the <strong><em>National referral general hospital</em></strong> of N’djamena. Poor hospital practices and factors associated to patients seem to be the most factors implicated in the SSI.展开更多
A method of Standard Site Method (SSM) in the American National Standards Institute’s ANSI C63.5 is described in the frequency ranges from 30 MHz to 1000 MHz. And a measurement system is set up for determining antenn...A method of Standard Site Method (SSM) in the American National Standards Institute’s ANSI C63.5 is described in the frequency ranges from 30 MHz to 1000 MHz. And a measurement system is set up for determining antenna factors (AF) of antennas on an Open Area Test Sites (OATS). AF of antennas including log-periodic antenna and biconical antenna is measured with SSM method by Shanghai Institute of Measurement and Testing Technology (SIMT), which shows good agreement to data measured by National Institute of Metrology (NIM). In the end, it analyzes the measurement uncertainty of SIMT in the 30 MHz to 1000 MHz frequency band and does comparison to that of NIM.展开更多
Transcription factors (TFs) are the core sentinels of gene regulation functioning by binding to highly specific DNA sequences to activate or repress the recruitment of RNA polymerase. The ability to identify transcrip...Transcription factors (TFs) are the core sentinels of gene regulation functioning by binding to highly specific DNA sequences to activate or repress the recruitment of RNA polymerase. The ability to identify transcription factor binding sites (TFBSs) is necessary to understand gene regulation and infer regulatory networks. Despite the fact that bioinformatics tools have been developed for years to improve computational identification of TFBSs, the accurate prediction still remains changeling as DNA motifs recognized by TFs are typically short and often lack obvious patterns. In this study we introduced a new attribute-motif distribution pattern (MDP) to assist in TFBS prediction. MDP was developed using a TF distribution pattern curve generated by analyzing 25 yeast TFs and 37 of their experimentally validated binding motifs, followed by calculating a scoring value to quantify the reliability of each motif prediction. Finally, MDP was tested using another set of 7 TFs with known binding sites to in silico validate the approach. The method was further tested in a non-yeast system using the filamentous fungus Magnaporthe oryzae transcription factor MoCRZ1. We demonstrate superior prediction reranking results using MDP over the commonly used program MEME and the other four predictors. The data showed significant improvements in the ranking of validated TFBS and provides a more sensitive statistics based approach for motif discovery.展开更多
A trapezoid valley site is chosen as a research site,and according to numerical models based on orthogonal design,the factors influencing ground motion in the valley site are studied with two-dimensional finite differ...A trapezoid valley site is chosen as a research site,and according to numerical models based on orthogonal design,the factors influencing ground motion in the valley site are studied with two-dimensional finite difference method. The influencing factors are ranked,and then the calculation results are verified by ground motion analysis. The conclusions are as follows:there are four factors that have important effects on ground motion of trapezoid valley sites,but the effects are different as the location of sites changes,the influencing factors rank differently with different site locations; The ranking of the influencing factors is the same for all the sites located within a distance of 40m from the valley's side,among them,the most effective one is the valley slope angle ranks,followed by depth-to-width ratio,overburden thickness,at last the input ground motion intensity.The impact of the factors on surface ground motion is roughly the same in the valley sites within a certain distance to the valley side,and the geometric parameters of the valley terrain play a greater part in influencing ground motion. With the increase of distance away from the valley's side,the ranking of the influencing factors also changes,the rating of slope angle moves backward,the ranking of the input ground motion and overburden thickness move ahead. The effect of valley geometric parameters on ground motions is gradually weakened,but the effect of other two influence factors are gradually increased,similar to cases of a horizontal layered site. Strong motion records in Anning River valley site were analyzed,and the results show that the valley topography has a significant amplification effect on ground motion,and that the numerical results of this paper are credible.展开更多
Background: Operative site infections (ISO) are typically nosocomial. According to the WHO the incidence of ISO varies from 0.5% to 15% and exceeds 25% in developing countries. They result from the combined action of ...Background: Operative site infections (ISO) are typically nosocomial. According to the WHO the incidence of ISO varies from 0.5% to 15% and exceeds 25% in developing countries. They result from the combined action of several factors and represent a concern for public health. To study the contributing factors of surgical site infections in patients operated at the University Clinic of Orthopedic Traumatology of CNHU-HKM of Cotonou. Method: This was an analytical cross-sectional study that involved 35 operated patients and ten nurses. Were included in our study: 1) All patients, without distinction of sex or age, having been operated on in the university clinic of orthopedic traumatology, during the survey period;2) Patients hospitalized for post-operative care during the survey period;3) The nursing staff on duty during our study period. The usual statistical measures were used according to the type of variables: means, standard deviations, percentages. Data analysis first involved calculating percentages for the qualitative variables and means followed by their standard deviations for the quantitative variables. Next, the Pearson Chi-square test was used to test the association between the dependent variable and the independent variables of interest. The significance level is set at p Results: The prevalence of surgical site infections was 28.58%. The main factors contributing to the occurrence of SSIs that were found were the patient’s level of education (p = 0.003) and the reuse of bandages used for dressing (p = 0.004). Other potential factors such as the economic status of the patient, the poor quality of technical dressings, and the preoperative stay were also highlighted. Conclusion: Surgical site infections remain a global concern. Risk factors were found on both the patient and nurse sides. It is urgent to address these various factors to minimize the occurrence of surgical site infections.展开更多
The objective of this study is to answer three main questions: What is the risk of wound infection for patients undergoing hip arthroplasty? What are the main etiologicagents of surgical site infection (SSI)? What are...The objective of this study is to answer three main questions: What is the risk of wound infection for patients undergoing hip arthroplasty? What are the main etiologicagents of surgical site infection (SSI)? What are the risk factors most associated with surgical site infection? Method: This was a multicentric, retrospective cohort study which analyzed data collected in five general hospitals in Belo Horizonte, Brazil, between the period of January 2009 and December 2013. The continuous parameters studied were age, length of hospital stay before surgery, duration of surgery, number of professionals at surgery and number of hospital admissions. Categorical variables were surgical wound classification (clean, clean contaminated, contaminated, dirty/infected), American Society of Anesthesiologists (ASA) score (I, II, III, IV, V), type of surgery (elective, emergency), general anesthesia (yes, no), prophylactic antibiotic (yes, no), trauma surgery (yes, no) and Nosocomial Infections Surveillance (NNIS) risk index (IRIC = 0, 1, 2, 3). Results: Estimated SSI risk was 3.2% (95% C.I. = 2.6% to 4.1%) and risk of osteomyelitis was 0.6% (95% C.I. = 0.4% to 1.1%). ASA score > 2, general anesthesia, length of hospital stay before surgery higher than four days, more than two professionals at surgical field and duration of surgery higher than five hours were risk factors for SSI after hip prosthesis (p < 0.05). The final multiple logistic regression analysis indicated that the modified NNIS risk was independently associated with surgical site infection after arthroplasty of hip. Conclusion: Despite the modified NNIS index being a risk factor for SSI, none of its independent variables was statistically significantly in the logistic model (p > 0.100). Each modified NNIS risk category increased the chance of a patient being infected by almost three times, when compared with the previous category (OR = 2.82;p = 0.011).展开更多
In this paper, the status quo of safety production on construction sites and the hazardous and harmful factors in the construction process were analyzed, and corresponding suggestions were proposed to make up for the ...In this paper, the status quo of safety production on construction sites and the hazardous and harmful factors in the construction process were analyzed, and corresponding suggestions were proposed to make up for the shortcomings of the safety production assurance system standards on construction sites.展开更多
文摘the investigation was carried out on 10-year-old plantation of Fraxinus mandshurica in Mao抏r Mountain Experimental Station of Northeast Forest University. Tree height (H), diameter at breast height (D1.3) and the increment of tree height in 5 years (H5), the thickness of humus layer, as well as the soil moisture were measured for the plantation and the growth indexes (H, D1.3, H5) for different site conditions were analyzed. The results showed that main site factors influencing the growth of Fraxinus mandshurica were soil moisture, gradient and location of slope in order. The growth of Fraxinus mandshurica was better on the middle- or up-slope site than on the down-slope site. Soil moisture and late frost caused by terrain are the main reasons that limit the growth of Fraxinus mandshurica plantation.
文摘Incidence of Armillaria infection was quantified based on site factors in New Zealand Pinus radiata plantations.A linear multiple regression model was derived to predict infection levels of Armillaria root rot.Factors positivily associated with the infection were:previous vegetation（native bush,pine）;soil type（pumice）;landform （valley,gully,flat）and the interaction between them.This model could assist in management planning with regard to the predisposition of particular stand to Armillaria infection.Keywods:Armillaria root rot,Disease incidence,Site factors,Quantification,Pinus radiata.
文摘Dahurian larch (Larix gmelinii Rupr.) Rupr is one of the dominant species in bothnatural and man-made forests in northeast China.Effects of site factors,including topography,soilproperties and nutrient elements(P,Ca,K,Mg,Mn,Zn,Cu,Ag,Co and Ni)on the growth of larchwere analyzed in detail,qualitatively and quantitatively.The ordination technique was used to groupthe site factors and to classify sites for larch.Analysis indicated that height growth,diameter(DBH)and volume of the larch varied among site types and this will be of theoretical and practical signifi-canee in developing larch plantations.
文摘Background: Cesarean sections have become increasingly prevalent in both developed and developing nations. Nevertheless, postoperative complications, including surgical site infections (SSIs), remain a substantial concern that contributes to heightened morbidity and mortality. This study is designed to evaluate the frequency and key determining factors associated with surgical site infections following cesarean section procedures in a tertiary care hospital in Bangladesh. Materials and Methods: This observational cross-sectional study, conducted at the Gynecology Department of Dhaka Medical College Hospital, involved 100 patients aged 15 - 45 who had cesarean deliveries. Data was collected during hospitalization and post-discharge and analyzed to determine the prevalence and relationship between socio-demographic characteristics and surgical site infection. Results: Among the study participants, 14% developed surgical site infections following cesarean operations. More than half of these patients were under the age of 25, with a mean age of 24.45 ± 4.44 years. Surgical site infections were more prevalent in individuals over 30 years old (P-value Conclusion: Post-cesarean surgical site infections are notably prevalent among the participants in this study. Several risk factors have been identified, including age, body mass index (BMI), socioeconomic status, anemia, preterm delivery, personal hygiene practices, regular menstrual cycles, and adherence to antenatal check-ups. The implementation of an effective awareness program, coupled with updated antibiotic protocols, is crucial for significantly reducing the incidence of these infections.
文摘Purpose: This paper aims to assess the incidences and risk factors for surgical site (ISO) infections in the general surgery department of the Koutiala District Hospital. Patients and Methods: This was a prospective and descriptive study from August 1, 2017 to October 31, 2020 involving all patients who underwent laparotomy in the general surgery department of the Koutiala District Hospital. Patients who were not operated on and who did not have a laparotomy were not included. Age, sex, frequency, patient history, National Nosocomial Infections Surveillance (NNIS) index, time to occur, bacteriological test results and course of infection were analyzed. Results: Fifty patients were registered. The average age was 34.2 ± 21.2 years old. Eleven patients were 60 years old or older. The hospital incidence rate was 4.3% and the community incidence rate was 6.1 cases per 100,000 population. The concept of smoking was noted in 15 patients. Patients were operated on in emergency 84% of cases. Peritonitis was the most common initial lesion with 32% of cases. Our patients had an NNIS index greater than 0 in 84% of cases. The time to develop infection from the surgical site was less than 3 days in 8 patients (16% of cases). Depending on the depth of the infection, it was superficial (cutaneous) in 39 patients (78%) and deep (subcutaneous and organic) in 11 patients (22%). Escherichia coli was the most common germ with 72% followed by staphylococcus aureus at 24%. We noted 22% morbidity. The median length of hospitalization was 9 ± 2.2 days. Conclusion: ISO is common in our service. Prevention and mastery require knowledge of risk factors.
基金funded through Engineering and Physical Sciences Research Council(EPSRC)[grant numbers EP/W005131/1,EP/V042556/1].
文摘Recent work analysing magnesium hydrogenation using Reflecting Electron Energy Loss Spectroscopy(REELS)and Density Function Theory(DFT)has indicated interfacial polarisation and interstitial hydrogen clustering influence the reaction rate.The site availability model has been modified to include interstitial hydrogen clustering within the site availability factor and interface polarisation using interface treatment.The new model,SAM-CV-S,has demonstrated improved modelling of magnesium hydrogenation across wide operating conditions,such as temperatures from 330 to 400℃and pressures up to 40 bar.This wide applicability makes it a robust model that can be used to simulate bed performance in solid-state hydrogen stores.Thus,the site availability factor successfully combines interstitial hydrogen clustering with thermal resistance effects,which are known to strongly influence metal hydride reactor designs at scale.The next phase of the model is to incorporate a predictive hydrogen capacity method into the model.
基金supported by funds from the National Key R&D Program of China (2016YFC0901603)the China 863 Program (2015AA020108)+1 种基金the State Key Laboratory of Protein and Plant Gene Researchsupported in part by the National Program for Support of Top-notch Young Professionals
文摘Understanding the functional effects of genetic variants is crucial in modern genomics and genetics. Transcription factor binding sites （TFBSs） are one of the most important cis-regulatory elements. While multiple tools have been developed to assess functional effects of genetic variants at TFBSs, they usually assume that each variant works in isolation and neglect the potential ＂interference＂ among multiple variants within the same TFBS. In this study, we presented COPE-TFBS （Context-Oriented Predictor for variant Effect on Transcription Factor Binding Site）, a novel method that considers sequence context to accurately predict variant effects on TFBSs. We systematically re-analyzed the sequencing data from both the 1000 Genomes Project and the Genotype-Tissue Expression （GTEx） Project via COPE-TFBS, and identified numbers of novel TFBSs, transformed TFBSs and discordantly annotated TFBSs resulting from multiple variants, further highlighting the necessity of sequence context in accurately annotating genetic variants.
基金Department of International Corporation of Yunnan Province and Tokyo University of Japan.
文摘The digital seismometers with high accuracy are installed at depths of -200 m, -100 m in borehole and on the surface in Dongchuan. By analysis of spectra ratio, the local events (with epicentral distances between 8.5 km and 95 km, magnitudes ML = 1.3-5.1) recorded are used to calculate site magnification factor at different depths (-100 m and surface) in Dongchuan. Comparing results with that at the depth of -200 m, the site magnification factors at three components of N-S, E-W and U-D corresponding to the depth of -100 m at 8 frequencies (f = 0.75, 1.0, 1.5, 2, 3, 4, 6, and 12 Hz) are 1.0-1.49 when 0.75 Hz- [less-than or equal to] f [less-than or equal to] 2 Hz and 0.47-0.99 when 2 Hz less than or equal f less than or equal 12 Hz. On the surface, site magnification factors at three components are all greater than 1, i.e. between 1.6 and 7.63. The correlation coefficients of site magnification factors for S wave and coda at the depth of -100 m and on surface are 0.82 and 0.81 when 0.75 Hz [less-than or equal to] f [less-than or equal to] 2 Hz, 0.47 and 0.45 when 2 Hz less than or equal f less than or equal 12 Hz, respectively.
文摘AIM To identify punitive transcriptional factor binding sites(TFBS) from regulatory single nucleotide polymorphisms(rS NPs) that are significantly associated with disease.METHODS The genome-wide association studies have provided us with nearly 6500 disease or trait-predisposing SNPs where 93% are located within non-coding regions such as gene regulatory or intergenic areas of the genome. In the regulatory region of a gene, a SNP can change the DNA sequence of a transcriptional factor(TF) motif and in turn may affect the process of gene regulation. SNP changes that affect gene expression and impact gene regulatory sequences such as promoters, enhancers, and silencers are known as rS NPs. Computational tools can be used to identify unique punitive TFBS created by rS NPs that are associated with disease or sickness. Computational analysis was used to identify punitive TFBS generated by the alleles of these rS NPs.RESULTS r SNPs within nine genes that have been significantly associated with disease or sickness were used to illustrate the tremendous diversity of punitive unique TFBS that can be generated by their alleles. The genes studied are the adrenergic, beta, receptor kinase 1, the v-akt murine thymoma viral oncogene homolog 3, the activating transcription factor 3, the type 2 demodkinase gene, the endothetal Per-Arnt-Sim domain protein 1, the lysosomal acid lipase A, the signal Transducer and Activator of Transcription 4, the thromboxane A2 receptor and the vascular endothelial growth factor A. From this sampling of SNPs among the nine genes, there are 73 potential unique TFBS generated by the common alleles comparedto 124 generated by the minor alleles indicating the tremendous diversity of potential TFs that are capable of regulating these genes.CONCLUSION From the diversity of unique punitive binding sites for TFs, it was found that some TFs play a role in the disease or sickness being studied.
文摘The risk to develop an infection after surgery depends on several factors. Those factors may be interdependent or not, depending on the nature of the surgery and the general condition of the patient. The aim of the present study was to identify the factors involved in the occurrence of the Surgical Site Infections (SSI) in the <strong><em>National referral general hospital </em></strong>of N’djamena (Chad). We first realized an interview in the emergency services and in the general surgery of the hospital. The information collected allowed us to draw up survey sheets. An investigation was then conducted on 152 patients who had surgery and were hospitalized during the study period. The frequency of the SSI was 33.6% (51/152). The main risk factors that we identified were the duration of hospitalization, the category of the hospitalization room, the urgent aspect of the surgical intervention, the patient’s nutritional status and the associated diseases with diabetes, which was present in 52.38% (11/21) of cases of associated pathologies. Analysis of the antibiotic prophylaxis administered to patients showed that ciprofloxacin was more effective in SSI prevention. Indeed, this antibiotic showed the fewest cases of infection with only 1.9% of patients having developed SSI. Our results show a very high frequency of SSI at the <strong><em>National referral general hospital</em></strong> of N’djamena. Poor hospital practices and factors associated to patients seem to be the most factors implicated in the SSI.
文摘A method of Standard Site Method (SSM) in the American National Standards Institute’s ANSI C63.5 is described in the frequency ranges from 30 MHz to 1000 MHz. And a measurement system is set up for determining antenna factors (AF) of antennas on an Open Area Test Sites (OATS). AF of antennas including log-periodic antenna and biconical antenna is measured with SSM method by Shanghai Institute of Measurement and Testing Technology (SIMT), which shows good agreement to data measured by National Institute of Metrology (NIM). In the end, it analyzes the measurement uncertainty of SIMT in the 30 MHz to 1000 MHz frequency band and does comparison to that of NIM.
文摘Transcription factors (TFs) are the core sentinels of gene regulation functioning by binding to highly specific DNA sequences to activate or repress the recruitment of RNA polymerase. The ability to identify transcription factor binding sites (TFBSs) is necessary to understand gene regulation and infer regulatory networks. Despite the fact that bioinformatics tools have been developed for years to improve computational identification of TFBSs, the accurate prediction still remains changeling as DNA motifs recognized by TFs are typically short and often lack obvious patterns. In this study we introduced a new attribute-motif distribution pattern (MDP) to assist in TFBS prediction. MDP was developed using a TF distribution pattern curve generated by analyzing 25 yeast TFs and 37 of their experimentally validated binding motifs, followed by calculating a scoring value to quantify the reliability of each motif prediction. Finally, MDP was tested using another set of 7 TFs with known binding sites to in silico validate the approach. The method was further tested in a non-yeast system using the filamentous fungus Magnaporthe oryzae transcription factor MoCRZ1. We demonstrate superior prediction reranking results using MDP over the commonly used program MEME and the other four predictors. The data showed significant improvements in the ranking of validated TFBS and provides a more sensitive statistics based approach for motif discovery.
基金sponsored by the National Natural Science Foundation of China(51508096)the Spark Program of Seismological Science and Technology,CEA(XH15067Y)
文摘A trapezoid valley site is chosen as a research site,and according to numerical models based on orthogonal design,the factors influencing ground motion in the valley site are studied with two-dimensional finite difference method. The influencing factors are ranked,and then the calculation results are verified by ground motion analysis. The conclusions are as follows:there are four factors that have important effects on ground motion of trapezoid valley sites,but the effects are different as the location of sites changes,the influencing factors rank differently with different site locations; The ranking of the influencing factors is the same for all the sites located within a distance of 40m from the valley's side,among them,the most effective one is the valley slope angle ranks,followed by depth-to-width ratio,overburden thickness,at last the input ground motion intensity.The impact of the factors on surface ground motion is roughly the same in the valley sites within a certain distance to the valley side,and the geometric parameters of the valley terrain play a greater part in influencing ground motion. With the increase of distance away from the valley's side,the ranking of the influencing factors also changes,the rating of slope angle moves backward,the ranking of the input ground motion and overburden thickness move ahead. The effect of valley geometric parameters on ground motions is gradually weakened,but the effect of other two influence factors are gradually increased,similar to cases of a horizontal layered site. Strong motion records in Anning River valley site were analyzed,and the results show that the valley topography has a significant amplification effect on ground motion,and that the numerical results of this paper are credible.
文摘Background: Operative site infections (ISO) are typically nosocomial. According to the WHO the incidence of ISO varies from 0.5% to 15% and exceeds 25% in developing countries. They result from the combined action of several factors and represent a concern for public health. To study the contributing factors of surgical site infections in patients operated at the University Clinic of Orthopedic Traumatology of CNHU-HKM of Cotonou. Method: This was an analytical cross-sectional study that involved 35 operated patients and ten nurses. Were included in our study: 1) All patients, without distinction of sex or age, having been operated on in the university clinic of orthopedic traumatology, during the survey period;2) Patients hospitalized for post-operative care during the survey period;3) The nursing staff on duty during our study period. The usual statistical measures were used according to the type of variables: means, standard deviations, percentages. Data analysis first involved calculating percentages for the qualitative variables and means followed by their standard deviations for the quantitative variables. Next, the Pearson Chi-square test was used to test the association between the dependent variable and the independent variables of interest. The significance level is set at p Results: The prevalence of surgical site infections was 28.58%. The main factors contributing to the occurrence of SSIs that were found were the patient’s level of education (p = 0.003) and the reuse of bandages used for dressing (p = 0.004). Other potential factors such as the economic status of the patient, the poor quality of technical dressings, and the preoperative stay were also highlighted. Conclusion: Surgical site infections remain a global concern. Risk factors were found on both the patient and nurse sides. It is urgent to address these various factors to minimize the occurrence of surgical site infections.
文摘The objective of this study is to answer three main questions: What is the risk of wound infection for patients undergoing hip arthroplasty? What are the main etiologicagents of surgical site infection (SSI)? What are the risk factors most associated with surgical site infection? Method: This was a multicentric, retrospective cohort study which analyzed data collected in five general hospitals in Belo Horizonte, Brazil, between the period of January 2009 and December 2013. The continuous parameters studied were age, length of hospital stay before surgery, duration of surgery, number of professionals at surgery and number of hospital admissions. Categorical variables were surgical wound classification (clean, clean contaminated, contaminated, dirty/infected), American Society of Anesthesiologists (ASA) score (I, II, III, IV, V), type of surgery (elective, emergency), general anesthesia (yes, no), prophylactic antibiotic (yes, no), trauma surgery (yes, no) and Nosocomial Infections Surveillance (NNIS) risk index (IRIC = 0, 1, 2, 3). Results: Estimated SSI risk was 3.2% (95% C.I. = 2.6% to 4.1%) and risk of osteomyelitis was 0.6% (95% C.I. = 0.4% to 1.1%). ASA score > 2, general anesthesia, length of hospital stay before surgery higher than four days, more than two professionals at surgical field and duration of surgery higher than five hours were risk factors for SSI after hip prosthesis (p < 0.05). The final multiple logistic regression analysis indicated that the modified NNIS risk was independently associated with surgical site infection after arthroplasty of hip. Conclusion: Despite the modified NNIS index being a risk factor for SSI, none of its independent variables was statistically significantly in the logistic model (p > 0.100). Each modified NNIS risk category increased the chance of a patient being infected by almost three times, when compared with the previous category (OR = 2.82;p = 0.011).
基金Sponsored by Research Fund of Binzhou College(BZXYG1817)Ph.D. Startup Funding Project of Binzhou College(2016Y33)Experimental Technology Funding Project of Binzhou College(BZXYSYXM201816)
文摘In this paper, the status quo of safety production on construction sites and the hazardous and harmful factors in the construction process were analyzed, and corresponding suggestions were proposed to make up for the shortcomings of the safety production assurance system standards on construction sites.
