To study the effects of growing rice （Oryza sativa L.） leaves under the treatment of the short-term elevated CO2 during the period of sink-source transition, several physiological processes such as dynamic changes i...To study the effects of growing rice （Oryza sativa L.） leaves under the treatment of the short-term elevated CO2 during the period of sink-source transition, several physiological processes such as dynamic changes in photosynthesis, photosynthate accumulation, enzyme activities （sucrose phosphate synthase （SPS）, and sucrose synthase （SS））, and their specific gene （spsl and RSusl） expressions in both mature and developing leaf were measured. Rice seedlings with fully expanded sixth leaf （marked as the source leaf, L6） were kept in elevated （700 μmol/mol） and ambient （350 mol/L） CO2 until the 7th leaf （marked as the sink leaf, L7） fully expanded. The results demonstrated that elevated CO2 significantly increased the rate of leaf elongation and biomass accumulation of L7 during the treatment without affecting the growth of L6. However, in both developing and mature leaves, net photosynthetic assimilation rate （A）, all kinds of photosynthate contents such as starch, sucrose and hexose, activities of SPS and SS and transcript levels of spsl and RSusl were significantly increased under elevated CO2 condition. Results suggested that the elevated CO2 had facilitated photosynthate assimilation, and increased photosynthate supplies from the source leaf to the sink leaf, which accelerated the growth and sink-source transition in new developing sink leaves. The mechanisms of SPS regulation by the elevated CO2 was also discussed.展开更多
To study whether integrative fertilization [growing milk vetch in winter and reducing the dose of chemical nitrogen(N) fertilizer] can improve rice yield, and to reveal the underlying regulatory mechanisms for integra...To study whether integrative fertilization [growing milk vetch in winter and reducing the dose of chemical nitrogen(N) fertilizer] can improve rice yield, and to reveal the underlying regulatory mechanisms for integrative fertilization, a three-year field trial including two treatments, milk vetch-rice-rice(MRR) and winter fallow-rice-rice(FRR), was conducted in 2010, 2011 and 2012.Our results demonstrated that the MRR treatment could significantly improve rice yield compared with the FRR treatment, especially when the application ratio of milk vetch and chemical fertilizer was 1:2.MRR treatment increased the effective panicle number and the spikelet number per panicle.In addition, a higher tillering number, leaf area index, photosynthetic-potential and photosynthetic-potential to grain ratio were observed in MRR treatment, which could provide enough dry matter for yield formation.Moreover, in MRR treatment, we discovered a higher transportation ratio and transformation ratio of dry matter in culm and leaves, and a stronger total sink capacity and spikelet-root bleeding intensity at the heading stage and 15 d after heading.Furthermore, the MRR treatment showed higher total N, phosphorus and potassium uptakes than FRR treatment, which was associated with the higher root dry weight in each soil layers.These results suggest that growing milk vetch in winter can improve rice yield under less chemical N fertilizer application, which is due to the improvement of soil nutrient status and the increased of rice root growth and development.展开更多
Removing fruit (RF) and retaining fruit (CK) were carried out during different phenological stages of fruit development onone-year-old shoot of Okubo peach trees [Prunus persica (L.) Batsch.] under preventing exportat...Removing fruit (RF) and retaining fruit (CK) were carried out during different phenological stages of fruit development onone-year-old shoot of Okubo peach trees [Prunus persica (L.) Batsch.] under preventing exportation of the assimilates tothe non experimental parts of the tree by girdling one-year-old shoot and keeping the same leaves between RF and CK.The results showed that fruit removal significantly decreased net photosynthetic rate (Pn), stomatal conductance (Gs)and transpiration rate (E), but significantly increased leaf surface temperature (TLeaf ) at about midday as compared with CK.Internal CO2 concentration, soluble sugar content, reductive sugar content, starch content except that during the finalrapid fruit growth stage, ADP-glucose pyrophosphorylase and amylase activities in source leaves were not significantlyaffected by fruit removal. There was a significantly positive parabolic correlation between Pn and Gs, and a strongpositive linear correlation between Pn and E. Moreover, Pn increased with increased TLeaf if TLeaf was below 38°C, thendecreased sharply when TLeaf exceeded the above critical temperature for both RF and CK. Pn of RF was lower, however,than that of CK in the same TLeaf , especially if TLeaf exceeded 38°C. It is suggested that the decreased stomatal aperture andincreased TLeaf may be the important mechanism in regulating photosynthesis under a decreased strength of sink demandby RF in fruit trees.展开更多
[Objective] The aim was to explore the role of coastal wetlands in phosphorus fixation in the Yellow River Delta.[Method] The research analyzed phosphorus behavior at sediment-water interface by static water column te...[Objective] The aim was to explore the role of coastal wetlands in phosphorus fixation in the Yellow River Delta.[Method] The research analyzed phosphorus behavior at sediment-water interface by static water column test. [Result] The research concluded that phosphorus concentration was in the range of 0.051-0.322 mg/L in overlying water, and phosphorus was effectively removed by degradation and adsorption. The static water column test has demonstrated that phosphorus concentration of coastal wetlands plays the role of accumulation when phosphorus concentration is lower than 7 mg/L in overlying water of coastal wetlands. It is notable that substrates of overlying water all perform well in adsorption, and the adsorption reaches saturation if the concentration goes up to 10 mg/L. [Conclusion] Coastal wetlands would significantly adsorb and degrade phosphorus in overlying water, controlling phosphorus within a lower range.展开更多
Understanding microbial community assembly in plants is critical for advancing agricultural sustainability.This study investigated microbial diversity and community assembly mechanisms across six compartments of tomat...Understanding microbial community assembly in plants is critical for advancing agricultural sustainability.This study investigated microbial diversity and community assembly mechanisms across six compartments of tomato plants:bulk soil,rhizosphere,root,stem,flower,and seed.Using 16S rRNA amplicon sequencing,we observed that microbial richness was highest in the bulk soil and rhizosphere,with significant reductions in internal plant tissues.Co‐occurrence network analysis identified distinct microbial hubs in each compartment,such as Bacillus in the root and seed,highlighting critical interactions influencing microbial dynamics.Ecological process modeling revealed that deterministic processes,such as selection,dominated in below‐ground compartments,whereas stochastic processes like drift were more influential in above‐ground tissues,reflecting differences in niche specificity and ecological stability.Dispersal limitation emerged as a key driver in soil‐associated compartments,structuring microbial diversity.These findings advance our understanding of the ecological mechanisms shaping plant microbiomes and suggest targeted microbiome management strategies to enhance crop health,productivity,and resilience.Future research integrating functional genomics,temporal dynamics,and environmental factors is necessary to uncover the broader implications of plant‐associated microbiomes.展开更多
基金the National Natural Science Foundation of China (30270800and 40231003).
文摘To study the effects of growing rice （Oryza sativa L.） leaves under the treatment of the short-term elevated CO2 during the period of sink-source transition, several physiological processes such as dynamic changes in photosynthesis, photosynthate accumulation, enzyme activities （sucrose phosphate synthase （SPS）, and sucrose synthase （SS））, and their specific gene （spsl and RSusl） expressions in both mature and developing leaf were measured. Rice seedlings with fully expanded sixth leaf （marked as the source leaf, L6） were kept in elevated （700 μmol/mol） and ambient （350 mol/L） CO2 until the 7th leaf （marked as the sink leaf, L7） fully expanded. The results demonstrated that elevated CO2 significantly increased the rate of leaf elongation and biomass accumulation of L7 during the treatment without affecting the growth of L6. However, in both developing and mature leaves, net photosynthetic assimilation rate （A）, all kinds of photosynthate contents such as starch, sucrose and hexose, activities of SPS and SS and transcript levels of spsl and RSusl were significantly increased under elevated CO2 condition. Results suggested that the elevated CO2 had facilitated photosynthate assimilation, and increased photosynthate supplies from the source leaf to the sink leaf, which accelerated the growth and sink-source transition in new developing sink leaves. The mechanisms of SPS regulation by the elevated CO2 was also discussed.
基金supported by the National Key Technology Research and Development Program(Grant No.2013BAD07B12)the grant from Jiangxi Province(555 Talents Program)
文摘To study whether integrative fertilization [growing milk vetch in winter and reducing the dose of chemical nitrogen(N) fertilizer] can improve rice yield, and to reveal the underlying regulatory mechanisms for integrative fertilization, a three-year field trial including two treatments, milk vetch-rice-rice(MRR) and winter fallow-rice-rice(FRR), was conducted in 2010, 2011 and 2012.Our results demonstrated that the MRR treatment could significantly improve rice yield compared with the FRR treatment, especially when the application ratio of milk vetch and chemical fertilizer was 1:2.MRR treatment increased the effective panicle number and the spikelet number per panicle.In addition, a higher tillering number, leaf area index, photosynthetic-potential and photosynthetic-potential to grain ratio were observed in MRR treatment, which could provide enough dry matter for yield formation.Moreover, in MRR treatment, we discovered a higher transportation ratio and transformation ratio of dry matter in culm and leaves, and a stronger total sink capacity and spikelet-root bleeding intensity at the heading stage and 15 d after heading.Furthermore, the MRR treatment showed higher total N, phosphorus and potassium uptakes than FRR treatment, which was associated with the higher root dry weight in each soil layers.These results suggest that growing milk vetch in winter can improve rice yield under less chemical N fertilizer application, which is due to the improvement of soil nutrient status and the increased of rice root growth and development.
基金This study was supported by the National Natural Science Foundation of China(30170654).
文摘Removing fruit (RF) and retaining fruit (CK) were carried out during different phenological stages of fruit development onone-year-old shoot of Okubo peach trees [Prunus persica (L.) Batsch.] under preventing exportation of the assimilates tothe non experimental parts of the tree by girdling one-year-old shoot and keeping the same leaves between RF and CK.The results showed that fruit removal significantly decreased net photosynthetic rate (Pn), stomatal conductance (Gs)and transpiration rate (E), but significantly increased leaf surface temperature (TLeaf ) at about midday as compared with CK.Internal CO2 concentration, soluble sugar content, reductive sugar content, starch content except that during the finalrapid fruit growth stage, ADP-glucose pyrophosphorylase and amylase activities in source leaves were not significantlyaffected by fruit removal. There was a significantly positive parabolic correlation between Pn and Gs, and a strongpositive linear correlation between Pn and E. Moreover, Pn increased with increased TLeaf if TLeaf was below 38°C, thendecreased sharply when TLeaf exceeded the above critical temperature for both RF and CK. Pn of RF was lower, however,than that of CK in the same TLeaf , especially if TLeaf exceeded 38°C. It is suggested that the decreased stomatal aperture andincreased TLeaf may be the important mechanism in regulating photosynthesis under a decreased strength of sink demandby RF in fruit trees.
基金Supported by Binzhou Foundation for Development of Science and Technology
文摘[Objective] The aim was to explore the role of coastal wetlands in phosphorus fixation in the Yellow River Delta.[Method] The research analyzed phosphorus behavior at sediment-water interface by static water column test. [Result] The research concluded that phosphorus concentration was in the range of 0.051-0.322 mg/L in overlying water, and phosphorus was effectively removed by degradation and adsorption. The static water column test has demonstrated that phosphorus concentration of coastal wetlands plays the role of accumulation when phosphorus concentration is lower than 7 mg/L in overlying water of coastal wetlands. It is notable that substrates of overlying water all perform well in adsorption, and the adsorption reaches saturation if the concentration goes up to 10 mg/L. [Conclusion] Coastal wetlands would significantly adsorb and degrade phosphorus in overlying water, controlling phosphorus within a lower range.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(RS‐2023‐00251252 and 2020R1A6A1A03047729)Rural Development Administration(RS‐2025‐02613089)Biomaterials Specialized Graduate Program through the Korea Environmental Industry&Technology Institute(KEITI)funded by the Ministry of Environment(MOE).
文摘Understanding microbial community assembly in plants is critical for advancing agricultural sustainability.This study investigated microbial diversity and community assembly mechanisms across six compartments of tomato plants:bulk soil,rhizosphere,root,stem,flower,and seed.Using 16S rRNA amplicon sequencing,we observed that microbial richness was highest in the bulk soil and rhizosphere,with significant reductions in internal plant tissues.Co‐occurrence network analysis identified distinct microbial hubs in each compartment,such as Bacillus in the root and seed,highlighting critical interactions influencing microbial dynamics.Ecological process modeling revealed that deterministic processes,such as selection,dominated in below‐ground compartments,whereas stochastic processes like drift were more influential in above‐ground tissues,reflecting differences in niche specificity and ecological stability.Dispersal limitation emerged as a key driver in soil‐associated compartments,structuring microbial diversity.These findings advance our understanding of the ecological mechanisms shaping plant microbiomes and suggest targeted microbiome management strategies to enhance crop health,productivity,and resilience.Future research integrating functional genomics,temporal dynamics,and environmental factors is necessary to uncover the broader implications of plant‐associated microbiomes.
