Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,con...Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,conventional MNs employ long needles(≥600μm),leading to pain and skin irritation.Moreover,it is critical to stably apply MNs against complex skin surfaces for uniform nanoscale drug delivery.Herein,a dually amplified transdermal patch(MN@EV/SC)is developed as the stem cell-derived EV delivery platform by hierarchically integrating an octopusinspired suction cup(SC)with short MNs(≤300μm).While leveraging the suction effect to induce nanoscale deformation of the stratum corneum,MN@EV/SC minimizes skin damage and enhances the adhesion of MNs,allowing EV to penetrate deeper into the dermis.When MNs of various lengths are applied to mouse skin,the short MNs can elicit comparable corticosterone release to chemical adhesives,whereas long MNs induce a prompt stress response.MN@EV/SC can achieve a remarkable penetration depth(290μm)for EV,compared to that of MN alone(111μm).Consequently,MN@EV/SC facilitates the revitalization of fibroblasts and enhances collagen synthesis in middle-aged mice.Overall,MN@EV/SC exhibits the potential for skin regeneration by modulating the dermal microenvironment and ensuring patient comfort.展开更多
Aconitines are a group of highly bioactive and toxic compounds found in aconitum species.[1]It is clinically used to treat rheumatism,rheumatoid arthritis,osteoarthritis,and cancer.[2]In folk medicine,aconitine is oft...Aconitines are a group of highly bioactive and toxic compounds found in aconitum species.[1]It is clinically used to treat rheumatism,rheumatoid arthritis,osteoarthritis,and cancer.[2]In folk medicine,aconitine is often soaked in white wine to make medicinal wine,which is used to treat rheumatism,joint pain,and other diseases.[3]Improper use of aconitine can cause adverse effects in the nervous system and digestive system,as well as cardiovascular dysfunction,especially arrhythmia.[4]Because of its high toxicity when it is improperly handled,aconitine has attracted widespread attention.展开更多
Objective:To evaluate the clinical efficacy of blood-letting cupping combined with manual lymphatic drainage in treating breast cancer-related lymphedema(BCRL)and explore its mechanism of action from both traditional ...Objective:To evaluate the clinical efficacy of blood-letting cupping combined with manual lymphatic drainage in treating breast cancer-related lymphedema(BCRL)and explore its mechanism of action from both traditional Chinese medicine and modern medical perspectives,providing a scientific basis and novel therapeutic approaches for clinical management of BCRL.Methods:Patients with BCRL admitted to the outpatient and inpatient departments of Hebei University Affiliated Hospital were enrolled.A prospective randomized controlled trial design was adopted,with eligible patients randomly assigned to a treatment group and a control group.The control group received manual lymphatic drainage alone,while the treatment group received manual lymphtic drainage combined with blood-letting cupping therapy.Posttreatment comparisons evaluated upper limb circumference reduction,edema severity grading,and upper limb functional scores.Vital signs and adverse reactions during treatment were recorded for both groups.Statistical software analyzed the data.Results:The treatment group demonstrated significantly greater reduction in upper limb circumference,improvement in edema severity,and higher upper limb function scores compared to the control group(P<0.05).Vital signs remained stable throughout treatment in both groups.No severe adverse reactions occurred in the treatment group;only isolated cases of mild skin itching were reported,which resolved after symptomatic management.Conclusion:The combination of bloodletting cupping and manual lymphatic drainage demonstrates reliable efficacy in treating BCRL,effectively alleviating edema symptoms and improving upper limb function with high safety.Its mechanism may relate to traditional Chinese medicine principles of“unblocking meridians,promoting blood circulation,and resolving stasis”and modern medical concepts of“enhancing local blood circulation,facilitating lymphatic drainage,and reducing inflammatory responses”.展开更多
The rapid increase in traffic loads and frequencies has rendered conventional asphalt pavement inadequate to maintain its durability under tropical climates.This challenge has necessitated the exploration of new sourc...The rapid increase in traffic loads and frequencies has rendered conventional asphalt pavement inadequate to maintain its durability under tropical climates.This challenge has necessitated the exploration of new sources of modified asphalt with enhanced stiffness and superior performance at high temperatures.Natural rubber(NR)is a renewable biopolymer that has received growing interest as a modifier for asphalt binders.Cup lump rubber(CLR),a type of NR,is used to enhance asphalt properties and improve the performance of road pavements.This study evaluates the influence of wax-based surfactants(WS)on CLR-modified asphalt binder(CMB).The assessment focuses on changes in chemical characteristics,rheological behaviour,activation energy,and morphology.Four concentrations of WS(0.1%,0.15%,0.2%,and 0.25%)were incorporated into CMB.Analysis of CMB chemical changes showed that viscosity increased due to higher sulfoxide,carbonyl,and aromatic bond indices.These chemical modifications contributed to improved resistance of the binder to heat-induced deterioration.In both unaged and aged CMB samples,the incorporation of WS reduced the sulfoxide index of the binder.Rheological analysis indicated that CMB improved rutting resistance and anti-ageing performance,while WS further enhanced fatigue resistance.Activation energy analysis suggested that the combination of CMB with 0.15%WS produced the most favourable enhancement.Micrograph results showed that WS improved binder homogeneity and interconnectivity.In conclusion,the findings indicated that incorporating 0.15%WS into CMB enhanced the performance and durability of the asphalt pavement.展开更多
In June 2026,the FIFA World Cup will be jointly hosted by three countries for the first time,featur ing 48 teams and 104 matches across 16 cities in North Amer ica.For the United States,Canada,and Mexico,this is not o...In June 2026,the FIFA World Cup will be jointly hosted by three countries for the first time,featur ing 48 teams and 104 matches across 16 cities in North Amer ica.For the United States,Canada,and Mexico,this is not only a sporting event but also a well-planned“economic feast.”展开更多
OBJECTIVE:To update the current best evidence on the effectiveness and safety of cupping therapy in pain management.METHODS:The protocol of this systematic review was registered at PROSPERO(CRD42021261308).An updated ...OBJECTIVE:To update the current best evidence on the effectiveness and safety of cupping therapy in pain management.METHODS:The protocol of this systematic review was registered at PROSPERO(CRD42021261308).An updated literature searching in 7 databases was conducted from January 2014 to January 2023.Two authors extracted data and assessed the risk of bias independently.Statistical analysis was performed using RevMan 5.4.1 software(Cochrane Collaboration,London,UK).Meta-analysis with a random effect model was conducted when there was no serious statistical heterogeneity among trials(I^(2) ≤ 75%).Grading of Recommendations Assessment,Development,and Evaluation was also conducted to assess the quality of evidence.RESULTS:Seventy-two trials with 5720 participants were included.All included trials were assessed as having high risk of bias.The majority of the included trials reported the benefit of cupping plus other therapy or cupping alone on improving cure rate(average risk ratio more than 1.15) and reducing visual analogue scale [average mean difference(MD) reduction 0.16 to 7.0 cm],improving quality of life,quality of sleep or other symptoms related to pain condition.And there was low/very low quality evidence that the incidence of adverse events in the cupping groups were lower than that in the control groups.Although the heterogeneity between studies and the methodological quality of the study itself lead to the low evidence strength of the current conclusions,the results of this study are a valuable supplement to the founding of previous review.CONCLUSION:Cupping therapy alone or combined with other therapy was considered benefit in relieving pain,improving the quality of life,and increasing the cure rate of patients with pain conditions,though supported by the low quality of evidence.According to the limited evidence,cupping therapy seems to have less harm than drugs when treating pain conditions.展开更多
BACKGROUND Clinical studies using Trabecular Titanium™acetabular cups have shown promising short and medium-term results.This material,due to its macro and micro surface roughness,provides a substrate for osseointegra...BACKGROUND Clinical studies using Trabecular Titanium™acetabular cups have shown promising short and medium-term results.This material,due to its macro and micro surface roughness,provides a substrate for osseointegration and enhances implant stability.However,there is a lack of evidence in the literature on the use of this material in patients with femoral neck fracture.AIM To evaluate the short-term clinical-functional and radiographic outcomes in patients with femoral neck fractures undergoing total hip arthroplasty(THA)with Trabecular Titanium™acetabular cup implants.METHODS The study included 104 patients with medial femoral neck fractures who underwent THA between January 2020 and December 2020 with the Delta TT acetabular cup(Lima Corporate,Villanova di San Daniele del Friuli,Italy).The mean age of the patients was 69.57±10.16 years(range:36-85 years).The followup period ranged from a minimum of 3 to a maximum of 4 years.Three questionnaires(Harris Hip Score,Oxford Hip Score,and EQ5D)were administered along with radiographic evaluations.Statistical methods included the Student's t-test and one-way analysis of variance for comparisons(with significance set at 0.05),and the Kaplan-Meier curve for prosthetic implant survival.RESULTS The mean follow-up was 41.5 months.The Harris Hip Score(HHS)showed a mean increase of 2.74 points(mean HHS 88.52 at 6 months postoperatively and mean HHS 91.26 at the last follow-up)with statistical significance.Similarly,the Oxford Hip Score demonstrated a statistically significant difference between follow-up groups.However,the EQ5D did not show statistically significant differences among the three groups(preoperative,6-month follow-up,and last follow-up).Revision surgery was required in 6 patients.According to Moore's criteria,96%of the acetabular components were radiographically stable and well-integrated at the last follow-up.The Kaplan-Meier curve showed a 96%survival rate.CONCLUSION The clinical and radiographic results obtained in the short to medium term confirm the excellent performance of the Delta TT acetabular cup in terms of osseointegration,providing an optimal solution both for young patients with high functional recovery demands and for fragile patients requiring optimal stability of the acetabular component to reduce the risk of implant failure.展开更多
Objective:Psoriasis,a common chronic inflammatory skin condition with genetic underpinnings,is traditionally managed with cupping therapy.Although used historically,the precise mechanical effects and therapeutic mecha...Objective:Psoriasis,a common chronic inflammatory skin condition with genetic underpinnings,is traditionally managed with cupping therapy.Although used historically,the precise mechanical effects and therapeutic mechanisms of cupping in psoriasis remain largely unexamined.This study aimed to evaluate cupping therapy's efficacy for psoriasis and investigate its role in modulating inflammatory responses and cellular metabolism.Methods:Psoriasis was induced in mice using topical imiquimod(IMQ).The effects of cupping on psoriatic lesions were assessed using the Psoriasis Area and Severity Index score,histology,immunohistochemistry,and immunofluorescence staining.polymerase chain reaction sequencing(RNA-seq)and Western blotting were conducted to examine changes in mRNA expression and the AMP-activated protein kinase(AMPK)signaling pathway.Results:Cupping therapy significantly reduced inflammation,epidermal thickness,and inflammatory cell infiltration in mice with IMQ-induced psoriasis.Immunohistochemistry and immunofluorescence showed lower expression of inflammatory markers and a shift in T-cell populations.RNA-seq and Western blotting indicated that cupping upregulated Piezo1 and activated the AMPK pathway,improving energy metabolism in psoriatic skin.Conclusion:Cupping therapy reduces epidermal hyperproliferation and inflammation in psoriasis,rebalancing the local immune microenvironment.Mechanistically,cupping promotes calcium influx via Piezo1,activates AMPK signaling,and supports metabolic homeostasis,suggesting therapeutic potential for psoriasis.展开更多
[Objectives]To observe the clinical efficacy of Mongolian medicine silver needle combined with cupping and bloodletting therapy in the treatment of cervical spondylotic radiculopathy(CSR).[Methods]A total of 120 patie...[Objectives]To observe the clinical efficacy of Mongolian medicine silver needle combined with cupping and bloodletting therapy in the treatment of cervical spondylotic radiculopathy(CSR).[Methods]A total of 120 patients diagnosed with CSR who received treatment at the International Mongolian Hospital of Inner Mongolia between October 2021 and October 2022 were selected and randomly divided into two groups:A test group and a control group,with 60 patients in each group.The patients in the test group received Mongolian medicine silver needle combined with cupping and bloodletting therapy,following the principles of syndrome differentiation in Mongolian medicine.In contrast,the patients in the control group received only the Mongolian medicine silver needle therapy.After treatment,the Mongolian medicine syndrome scores,visual analogue scale(VAS)scores,and total clinical therapeutic effects in the two groups of patients were compared both prior to and following the treatment.[Results]The improvement in VAS and Mongolian medicine syndrome scores in the test group was greater than that in the control group,with a statistically significant difference(P<0.05).The scores of the test group surpassed those of the control group,and this difference was also statistically significant(P<0.05).Furthermore,the overall therapeutic effect in the test group was 93.33%,which was markedly higher than 76.67%observed in the control group(P<0.05).[Conclusions]The therapeutic effect of Mongolian medicine silver needle combined with cupping and bloodletting therapy in the treatment of CSR is remarkable.This approach has demonstrated efficacy in alleviating patient pain and improving the functionality of the cervical vertebrae,thereby warranting clinical promotion and application.展开更多
AIM:To explore the impact of insulin-like growth factor-1 receptorα(IGF-1Rα)on the differentiation fate of optic-cupderived retinal stem cells(OC-RSCs)into retinal ganglion cells(RGCs)in vitro.METHODS:OC-RSCs were i...AIM:To explore the impact of insulin-like growth factor-1 receptorα(IGF-1Rα)on the differentiation fate of optic-cupderived retinal stem cells(OC-RSCs)into retinal ganglion cells(RGCs)in vitro.METHODS:OC-RSCs were isolated from optic cups of rats on embryonic day 12.5,and high-purity OC-RSCs were obtained by conditioned culture and passage.Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry,and the serum concentration with high interference with differentiation ratio was selected.Furthermore,the effect of blocking IGF-1Rαon the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.RESULTS:Immunohistochemical analysis revealed IGF-1Rαwas highly expressed in rat embryos at day 12.5.OC-RSCs were isolated and purified,and high-purity OCRSCs were obtained.When 2.5%serum was administered,the ratio of differentiated RGCs(Thy-1.1 positive)decreased significantly,and the results of immunoblotting also confirmed the blockade of IGF-1Rαreduced Thy-1.1 protein expression.CONCLUSION:IGF-1Rαblocking can reduce the differentiation of OC-RSCs into RGCs.展开更多
基金supported by National Research Foundation of Korea(NRF)grants funded by the Korean government(MSIT)(No.RS-2023-00256265,RS-2024-00352352,RS-2024-00405818)the Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korea government(the Ministry of Science and ICT,the Ministry of Health&Welfare).(No.25A0102L1)support from the Market-led K-sensor technology program(RS-2022-00154781,Development of large-area wafer-level flexible/stretchable hybrid sensor platform technology for form factor-free highly integrated convergence sensor),funded By the Ministry of Trade,Industry&Energy(MOTIE,Korea).
文摘Microneedles(MNs)have been extensively investigated for transdermal delivery of large-sized drugs,including proteins,nucleic acids,and even extracellular vesicles(EVs).However,for their sufficient skin penetration,conventional MNs employ long needles(≥600μm),leading to pain and skin irritation.Moreover,it is critical to stably apply MNs against complex skin surfaces for uniform nanoscale drug delivery.Herein,a dually amplified transdermal patch(MN@EV/SC)is developed as the stem cell-derived EV delivery platform by hierarchically integrating an octopusinspired suction cup(SC)with short MNs(≤300μm).While leveraging the suction effect to induce nanoscale deformation of the stratum corneum,MN@EV/SC minimizes skin damage and enhances the adhesion of MNs,allowing EV to penetrate deeper into the dermis.When MNs of various lengths are applied to mouse skin,the short MNs can elicit comparable corticosterone release to chemical adhesives,whereas long MNs induce a prompt stress response.MN@EV/SC can achieve a remarkable penetration depth(290μm)for EV,compared to that of MN alone(111μm).Consequently,MN@EV/SC facilitates the revitalization of fibroblasts and enhances collagen synthesis in middle-aged mice.Overall,MN@EV/SC exhibits the potential for skin regeneration by modulating the dermal microenvironment and ensuring patient comfort.
基金supported by grants from the National Natural Science Foundation of China(82360387)the Key Laboratory of Emergency Medicine of Yunnan Province(202449CE340015).
文摘Aconitines are a group of highly bioactive and toxic compounds found in aconitum species.[1]It is clinically used to treat rheumatism,rheumatoid arthritis,osteoarthritis,and cancer.[2]In folk medicine,aconitine is often soaked in white wine to make medicinal wine,which is used to treat rheumatism,joint pain,and other diseases.[3]Improper use of aconitine can cause adverse effects in the nervous system and digestive system,as well as cardiovascular dysfunction,especially arrhythmia.[4]Because of its high toxicity when it is improperly handled,aconitine has attracted widespread attention.
文摘Objective:To evaluate the clinical efficacy of blood-letting cupping combined with manual lymphatic drainage in treating breast cancer-related lymphedema(BCRL)and explore its mechanism of action from both traditional Chinese medicine and modern medical perspectives,providing a scientific basis and novel therapeutic approaches for clinical management of BCRL.Methods:Patients with BCRL admitted to the outpatient and inpatient departments of Hebei University Affiliated Hospital were enrolled.A prospective randomized controlled trial design was adopted,with eligible patients randomly assigned to a treatment group and a control group.The control group received manual lymphatic drainage alone,while the treatment group received manual lymphtic drainage combined with blood-letting cupping therapy.Posttreatment comparisons evaluated upper limb circumference reduction,edema severity grading,and upper limb functional scores.Vital signs and adverse reactions during treatment were recorded for both groups.Statistical software analyzed the data.Results:The treatment group demonstrated significantly greater reduction in upper limb circumference,improvement in edema severity,and higher upper limb function scores compared to the control group(P<0.05).Vital signs remained stable throughout treatment in both groups.No severe adverse reactions occurred in the treatment group;only isolated cases of mild skin itching were reported,which resolved after symptomatic management.Conclusion:The combination of bloodletting cupping and manual lymphatic drainage demonstrates reliable efficacy in treating BCRL,effectively alleviating edema symptoms and improving upper limb function with high safety.Its mechanism may relate to traditional Chinese medicine principles of“unblocking meridians,promoting blood circulation,and resolving stasis”and modern medical concepts of“enhancing local blood circulation,facilitating lymphatic drainage,and reducing inflammatory responses”.
基金the financial support provided under the Fundamental Research Grant Scheme(FRGS),Project Code:FRGS/1/2021/TK01/USM/02/1,which made this research possible,The kind assistance and collaboration of all material suppliers were also gratefully acknowledged.Additional appreciation is extended to the Public Works Department Malaysia for the scholarship provided through the Hadiah Latihan Persekutuan programme for professional and managerial officers pursuing doctoral studies(JPA-1-840622086040)The APC was funded by Prof.Hui Yao from Beijing University of Technology.
文摘The rapid increase in traffic loads and frequencies has rendered conventional asphalt pavement inadequate to maintain its durability under tropical climates.This challenge has necessitated the exploration of new sources of modified asphalt with enhanced stiffness and superior performance at high temperatures.Natural rubber(NR)is a renewable biopolymer that has received growing interest as a modifier for asphalt binders.Cup lump rubber(CLR),a type of NR,is used to enhance asphalt properties and improve the performance of road pavements.This study evaluates the influence of wax-based surfactants(WS)on CLR-modified asphalt binder(CMB).The assessment focuses on changes in chemical characteristics,rheological behaviour,activation energy,and morphology.Four concentrations of WS(0.1%,0.15%,0.2%,and 0.25%)were incorporated into CMB.Analysis of CMB chemical changes showed that viscosity increased due to higher sulfoxide,carbonyl,and aromatic bond indices.These chemical modifications contributed to improved resistance of the binder to heat-induced deterioration.In both unaged and aged CMB samples,the incorporation of WS reduced the sulfoxide index of the binder.Rheological analysis indicated that CMB improved rutting resistance and anti-ageing performance,while WS further enhanced fatigue resistance.Activation energy analysis suggested that the combination of CMB with 0.15%WS produced the most favourable enhancement.Micrograph results showed that WS improved binder homogeneity and interconnectivity.In conclusion,the findings indicated that incorporating 0.15%WS into CMB enhanced the performance and durability of the asphalt pavement.
文摘In June 2026,the FIFA World Cup will be jointly hosted by three countries for the first time,featur ing 48 teams and 104 matches across 16 cities in North Amer ica.For the United States,Canada,and Mexico,this is not only a sporting event but also a well-planned“economic feast.”
基金National Natural Science Foundation of China Project:Research on the Correlation between Traditional Chinese Medicine Constitution,Cupping Marks Color,and Clinical Efficacy Based on Regression Models (No.81804000)。
文摘OBJECTIVE:To update the current best evidence on the effectiveness and safety of cupping therapy in pain management.METHODS:The protocol of this systematic review was registered at PROSPERO(CRD42021261308).An updated literature searching in 7 databases was conducted from January 2014 to January 2023.Two authors extracted data and assessed the risk of bias independently.Statistical analysis was performed using RevMan 5.4.1 software(Cochrane Collaboration,London,UK).Meta-analysis with a random effect model was conducted when there was no serious statistical heterogeneity among trials(I^(2) ≤ 75%).Grading of Recommendations Assessment,Development,and Evaluation was also conducted to assess the quality of evidence.RESULTS:Seventy-two trials with 5720 participants were included.All included trials were assessed as having high risk of bias.The majority of the included trials reported the benefit of cupping plus other therapy or cupping alone on improving cure rate(average risk ratio more than 1.15) and reducing visual analogue scale [average mean difference(MD) reduction 0.16 to 7.0 cm],improving quality of life,quality of sleep or other symptoms related to pain condition.And there was low/very low quality evidence that the incidence of adverse events in the cupping groups were lower than that in the control groups.Although the heterogeneity between studies and the methodological quality of the study itself lead to the low evidence strength of the current conclusions,the results of this study are a valuable supplement to the founding of previous review.CONCLUSION:Cupping therapy alone or combined with other therapy was considered benefit in relieving pain,improving the quality of life,and increasing the cure rate of patients with pain conditions,though supported by the low quality of evidence.According to the limited evidence,cupping therapy seems to have less harm than drugs when treating pain conditions.
文摘BACKGROUND Clinical studies using Trabecular Titanium™acetabular cups have shown promising short and medium-term results.This material,due to its macro and micro surface roughness,provides a substrate for osseointegration and enhances implant stability.However,there is a lack of evidence in the literature on the use of this material in patients with femoral neck fracture.AIM To evaluate the short-term clinical-functional and radiographic outcomes in patients with femoral neck fractures undergoing total hip arthroplasty(THA)with Trabecular Titanium™acetabular cup implants.METHODS The study included 104 patients with medial femoral neck fractures who underwent THA between January 2020 and December 2020 with the Delta TT acetabular cup(Lima Corporate,Villanova di San Daniele del Friuli,Italy).The mean age of the patients was 69.57±10.16 years(range:36-85 years).The followup period ranged from a minimum of 3 to a maximum of 4 years.Three questionnaires(Harris Hip Score,Oxford Hip Score,and EQ5D)were administered along with radiographic evaluations.Statistical methods included the Student's t-test and one-way analysis of variance for comparisons(with significance set at 0.05),and the Kaplan-Meier curve for prosthetic implant survival.RESULTS The mean follow-up was 41.5 months.The Harris Hip Score(HHS)showed a mean increase of 2.74 points(mean HHS 88.52 at 6 months postoperatively and mean HHS 91.26 at the last follow-up)with statistical significance.Similarly,the Oxford Hip Score demonstrated a statistically significant difference between follow-up groups.However,the EQ5D did not show statistically significant differences among the three groups(preoperative,6-month follow-up,and last follow-up).Revision surgery was required in 6 patients.According to Moore's criteria,96%of the acetabular components were radiographically stable and well-integrated at the last follow-up.The Kaplan-Meier curve showed a 96%survival rate.CONCLUSION The clinical and radiographic results obtained in the short to medium term confirm the excellent performance of the Delta TT acetabular cup in terms of osseointegration,providing an optimal solution both for young patients with high functional recovery demands and for fragile patients requiring optimal stability of the acetabular component to reduce the risk of implant failure.
基金National Natural Science Foundation of China(Nos.82004366,82474047 and 82474369)the High-level TCM Key Discipline Construction Project(Integrative Chinese and Western Medicine)of the National Administration of TCM(No.zyyzdxk-2023065)+3 种基金Pudong New Area Health System Leading Talents Program(No.PWRd2024-18)Pudong New Area Featured Discipline(Traditional Chinese Medicine Dermatology)(No.YC-2023-0609)the Sailing Program of the Shanghai RisingStar Program(No.22YF1449700)the Youth Talent Support Project of the China Society of TCM(No.2023-QNRC2-B25)。
文摘Objective:Psoriasis,a common chronic inflammatory skin condition with genetic underpinnings,is traditionally managed with cupping therapy.Although used historically,the precise mechanical effects and therapeutic mechanisms of cupping in psoriasis remain largely unexamined.This study aimed to evaluate cupping therapy's efficacy for psoriasis and investigate its role in modulating inflammatory responses and cellular metabolism.Methods:Psoriasis was induced in mice using topical imiquimod(IMQ).The effects of cupping on psoriatic lesions were assessed using the Psoriasis Area and Severity Index score,histology,immunohistochemistry,and immunofluorescence staining.polymerase chain reaction sequencing(RNA-seq)and Western blotting were conducted to examine changes in mRNA expression and the AMP-activated protein kinase(AMPK)signaling pathway.Results:Cupping therapy significantly reduced inflammation,epidermal thickness,and inflammatory cell infiltration in mice with IMQ-induced psoriasis.Immunohistochemistry and immunofluorescence showed lower expression of inflammatory markers and a shift in T-cell populations.RNA-seq and Western blotting indicated that cupping upregulated Piezo1 and activated the AMPK pathway,improving energy metabolism in psoriatic skin.Conclusion:Cupping therapy reduces epidermal hyperproliferation and inflammation in psoriasis,rebalancing the local immune microenvironment.Mechanistically,cupping promotes calcium influx via Piezo1,activates AMPK signaling,and supports metabolic homeostasis,suggesting therapeutic potential for psoriasis.
基金Supported by Project of Science and Technology Department of Inner Mongolia Autonomous Region(2019GG125).
文摘[Objectives]To observe the clinical efficacy of Mongolian medicine silver needle combined with cupping and bloodletting therapy in the treatment of cervical spondylotic radiculopathy(CSR).[Methods]A total of 120 patients diagnosed with CSR who received treatment at the International Mongolian Hospital of Inner Mongolia between October 2021 and October 2022 were selected and randomly divided into two groups:A test group and a control group,with 60 patients in each group.The patients in the test group received Mongolian medicine silver needle combined with cupping and bloodletting therapy,following the principles of syndrome differentiation in Mongolian medicine.In contrast,the patients in the control group received only the Mongolian medicine silver needle therapy.After treatment,the Mongolian medicine syndrome scores,visual analogue scale(VAS)scores,and total clinical therapeutic effects in the two groups of patients were compared both prior to and following the treatment.[Results]The improvement in VAS and Mongolian medicine syndrome scores in the test group was greater than that in the control group,with a statistically significant difference(P<0.05).The scores of the test group surpassed those of the control group,and this difference was also statistically significant(P<0.05).Furthermore,the overall therapeutic effect in the test group was 93.33%,which was markedly higher than 76.67%observed in the control group(P<0.05).[Conclusions]The therapeutic effect of Mongolian medicine silver needle combined with cupping and bloodletting therapy in the treatment of CSR is remarkable.This approach has demonstrated efficacy in alleviating patient pain and improving the functionality of the cervical vertebrae,thereby warranting clinical promotion and application.
文摘AIM:To explore the impact of insulin-like growth factor-1 receptorα(IGF-1Rα)on the differentiation fate of optic-cupderived retinal stem cells(OC-RSCs)into retinal ganglion cells(RGCs)in vitro.METHODS:OC-RSCs were isolated from optic cups of rats on embryonic day 12.5,and high-purity OC-RSCs were obtained by conditioned culture and passage.Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry,and the serum concentration with high interference with differentiation ratio was selected.Furthermore,the effect of blocking IGF-1Rαon the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.RESULTS:Immunohistochemical analysis revealed IGF-1Rαwas highly expressed in rat embryos at day 12.5.OC-RSCs were isolated and purified,and high-purity OCRSCs were obtained.When 2.5%serum was administered,the ratio of differentiated RGCs(Thy-1.1 positive)decreased significantly,and the results of immunoblotting also confirmed the blockade of IGF-1Rαreduced Thy-1.1 protein expression.CONCLUSION:IGF-1Rαblocking can reduce the differentiation of OC-RSCs into RGCs.
