Breastfeeding is drastically decreasing over time and nipple trauma constitutes one of the most significant reasons for its discontinuation. In this context, this study aimed at evaluating the effectiveness and tolera...Breastfeeding is drastically decreasing over time and nipple trauma constitutes one of the most significant reasons for its discontinuation. In this context, this study aimed at evaluating the effectiveness and tolerability of a topical treatment device, namely Silver Cap<sup>®</sup> (Depofarma S.P.A.), when used to prevent pain and nipple fissure formation. The medical device consists in a silver trilaminate cup to be placed on the nipple, providing physical protection and creating a moist environment. The study involved 187 women: 38 started to apply the device prior to lactation (PL group) and 149 during lactation (DL group). Aiming to collect safety and performance data, both groups were provided with questionnaires during 6 consecutive visits (120 days of follow-up). At last visit, absence of painful symptoms was reported by the 98.8% and the 100% of women in DL and PL groups, respectively. Moreover, no nipple fissures were observed and no adverse events directly correlated to use of the device were reported. According to these findings, it can be concluded that Silver Cap<sup>®</sup> is a safe and effective device for nipple pain and fissure prevention. .展开更多
Silver nanorods have been successfully synthesized in large scale by the ethylene glycol（EG） reduction in the presence of ionic liquid（IL） 1-butyl-3-methylimidazolium tetrafluoroborate（bmimBF_4） and polyvinyl-py...Silver nanorods have been successfully synthesized in large scale by the ethylene glycol（EG） reduction in the presence of ionic liquid（IL） 1-butyl-3-methylimidazolium tetrafluoroborate（bmimBF_4） and polyvinyl-pyrrolidone（PVP）.The silver nanorods were characterized by scanning electron microscopy（SEM）,high-resolution transmission electron microscopy（HRTEM）,transmission electron microscopy（TEM）,electron energy disperse spectroscopy（EDS） and UV-vis spectroscopy.The results showed that the uniform silver nanorods have an average diameter of about 100 nm and the aspect ratio from 15 to 20.IL,bmimBF_4 may play a role of capping agent together with PVP in the formation of silver nanorods.On the other band,bmimBF_4 may accelerate nucleation and improve the stability of the resulting Ag nanorods due to the low interface tension of IL.展开更多
Conventional synthesis of silver nanoparticles employs a reducing agent and a capping agent. Surfactants are effec-tive capping agents as they prevent the aggregation of nanoparticles during storage and use. However, ...Conventional synthesis of silver nanoparticles employs a reducing agent and a capping agent. Surfactants are effec-tive capping agents as they prevent the aggregation of nanoparticles during storage and use. However, the biocompatibility of several of the surfactants is questionable. In this report, the use of thiosalicylic acid as both reducing and capping agent is reported. Compared to conventional synthesis, this methodology requires higher temperature for synthesis, which then is expected to result in aggregates of larger size. The ability of three different synthesis methodologies – direct heating, photochemical and microwave dielectric treatment were evaluated and assessed on the basis of the size, size distribution and stability of the particles. Microwave irradiation was found to be most suitable for achieving particles with a hydrodynamic diameter of 10 nm. Our studies indicate that -COO- group is involved in the reduction of Ag+ and –SH group of TSA is involved in the capping of the nanoparticles.展开更多
Biopolymer based hydrogels are highly adaptable, compatible and have shown great potential in biological tissues in biomedical applications. However, the development of bio-based hydrogels with high strength and effec...Biopolymer based hydrogels are highly adaptable, compatible and have shown great potential in biological tissues in biomedical applications. However, the development of bio-based hydrogels with high strength and effective antibacterial activity remains challenging. Herein, a series of vanillin-cross-linked chitosan nanocomposite hydrogel interfacially reinforced by g-C_(3)N_(4)nanosheet carrying starch-caped Ag NPs were prepared for wound healing applications. The study aimed to enhance the strength, sustainability and control release ability of the fabricated membranes. Starch-caped silver nanoparticles were incorporated to enhance the anti-bacterial activities The fabricated membranes were assessed using various characterization techniques such as FT-IR, XRD, SEM, mechanical testing, Gel fraction and porosity alongside traditional biomedical tests i.e., swelling percentage, moisture retention ability, water vapor transmission rate, oxygen permeability, anti-bacterial activity and drug-release of the fabricated membranes. The mechanical strength reached as high as 25.9 ± 0.24 MPa for the best optimized sample. The moisture retention lied between 87%–89%, gel fraction 80%–85%, and water vapor transmission up to 104 ± 1.9g m^(-2)h^(-1)showing great properties of the fabricated membrane. Swelling percentage surged to 225% for blood while porosity fluctuated between 44% ± 2.1% and 52.5% ± 2.3%. Oxygen permeability reached up to 8.02 mg/L showing the breathable nature of fabricated membranes. The nanocomposite membrane shown excellent antibacterial activity for both gram-positive and gram-negative bacteria with a maximum zone of inhibition 30 ± 0.25 mm and 36.23 ± 0.23 mm respectively. Furthermore, nanoparticles maintained sustainable release following non-fickian diffusion. The fabricated membrane demonstrated the application of inorganic filler to enhance the strength of biopolymer hydrogel with superior properties.These results envisage the potential of synthesized membrane to be used as wound dressing, artificial skin and load-bearing scaffolds.展开更多
Silver nanoparticles(AgNPs)are of potential interest because of their effective antibacterial and antiviral activities.Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs.There ...Silver nanoparticles(AgNPs)are of potential interest because of their effective antibacterial and antiviral activities.Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs.There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy.Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method;the size of Cit-AgNPs was determined by an atomic force microscope(AFM)and was between 15-90 nm.Acinetobacter baumannii(A.baumannii)isolates were the only sensitive species to Cit-AgNPs.MICs and MBC of Cit-AgNPs were determined by using A.baumannii.The results showed an additive effect of Cit-AgNPs.Four mice groups were infected with a sub-lethal dose of A.baumannii intraperitoneally,IP.The single daily dose of Cit-AgNPs and imipenem plus Cit-AgNPs combination were administered IP.Imipenem and phosphate buffer saline(PBS)was used as positive control and negative control,respectively.Interestingly,only the PBS-treated group showed growth of A.baumannii in the liver and spleen of sacrificed mice.Histopathologically,Cit-AgNPs showed antibacterial activity and had an additive effect when combined with imipenem in vivo and in vitro.Moreover,the Cit-AgNPs showed dose-dependent activity and the organs differed in the illumination of the toxicity effect of Cit-AgNPs even after high dose administration.In conclusion,Cit-capped AgNPs had antibacterial activity against multiple drug resistant(MDR)A.baumannii but not against K.pneumoniae and E.coli.Cit-capped AgNPs increased the inhibition zone of imipenem in additive effect;the minimum inhibitory concentration and the minimum bactericidal concentration of Cit-capped AgNPs were relatively low.Cit-capped AgNPs eliminated A.baumannii infection in vivo when it was given alone or in combination with imipenem.The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed in the illumination of the inflammatory effect of Cit-AgNPs after high dose administration.It is not recommended to use Cit-capped AgNPs systemically despite their valuable additive antibacterial effect especially with a high dose and the combination with imipenem,Topical administration needs to be evaluated.展开更多
文摘Breastfeeding is drastically decreasing over time and nipple trauma constitutes one of the most significant reasons for its discontinuation. In this context, this study aimed at evaluating the effectiveness and tolerability of a topical treatment device, namely Silver Cap<sup>®</sup> (Depofarma S.P.A.), when used to prevent pain and nipple fissure formation. The medical device consists in a silver trilaminate cup to be placed on the nipple, providing physical protection and creating a moist environment. The study involved 187 women: 38 started to apply the device prior to lactation (PL group) and 149 during lactation (DL group). Aiming to collect safety and performance data, both groups were provided with questionnaires during 6 consecutive visits (120 days of follow-up). At last visit, absence of painful symptoms was reported by the 98.8% and the 100% of women in DL and PL groups, respectively. Moreover, no nipple fissures were observed and no adverse events directly correlated to use of the device were reported. According to these findings, it can be concluded that Silver Cap<sup>®</sup> is a safe and effective device for nipple pain and fissure prevention. .
基金supported by the National Natural Science Foundation of China(No.20773081 and 20873074)National Basic Research Program(No.2007CB808004 and 2009CB930101)Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering,TIPC,CAS.
文摘Silver nanorods have been successfully synthesized in large scale by the ethylene glycol（EG） reduction in the presence of ionic liquid（IL） 1-butyl-3-methylimidazolium tetrafluoroborate（bmimBF_4） and polyvinyl-pyrrolidone（PVP）.The silver nanorods were characterized by scanning electron microscopy（SEM）,high-resolution transmission electron microscopy（HRTEM）,transmission electron microscopy（TEM）,electron energy disperse spectroscopy（EDS） and UV-vis spectroscopy.The results showed that the uniform silver nanorods have an average diameter of about 100 nm and the aspect ratio from 15 to 20.IL,bmimBF_4 may play a role of capping agent together with PVP in the formation of silver nanorods.On the other band,bmimBF_4 may accelerate nucleation and improve the stability of the resulting Ag nanorods due to the low interface tension of IL.
文摘Conventional synthesis of silver nanoparticles employs a reducing agent and a capping agent. Surfactants are effec-tive capping agents as they prevent the aggregation of nanoparticles during storage and use. However, the biocompatibility of several of the surfactants is questionable. In this report, the use of thiosalicylic acid as both reducing and capping agent is reported. Compared to conventional synthesis, this methodology requires higher temperature for synthesis, which then is expected to result in aggregates of larger size. The ability of three different synthesis methodologies – direct heating, photochemical and microwave dielectric treatment were evaluated and assessed on the basis of the size, size distribution and stability of the particles. Microwave irradiation was found to be most suitable for achieving particles with a hydrodynamic diameter of 10 nm. Our studies indicate that -COO- group is involved in the reduction of Ag+ and –SH group of TSA is involved in the capping of the nanoparticles.
基金supported by the National Natural Science Foundation of China (No. 52003113)Guangdong Basic and Applied Basic Research Foundation (Nos. 2021A1515010745,2020A1515110356)+1 种基金Science and Technology Projects of Guangzhou City (No. 202102020359)the financial support from the China Postdoctoral Science Foundation (No.F121280003)。
文摘Biopolymer based hydrogels are highly adaptable, compatible and have shown great potential in biological tissues in biomedical applications. However, the development of bio-based hydrogels with high strength and effective antibacterial activity remains challenging. Herein, a series of vanillin-cross-linked chitosan nanocomposite hydrogel interfacially reinforced by g-C_(3)N_(4)nanosheet carrying starch-caped Ag NPs were prepared for wound healing applications. The study aimed to enhance the strength, sustainability and control release ability of the fabricated membranes. Starch-caped silver nanoparticles were incorporated to enhance the anti-bacterial activities The fabricated membranes were assessed using various characterization techniques such as FT-IR, XRD, SEM, mechanical testing, Gel fraction and porosity alongside traditional biomedical tests i.e., swelling percentage, moisture retention ability, water vapor transmission rate, oxygen permeability, anti-bacterial activity and drug-release of the fabricated membranes. The mechanical strength reached as high as 25.9 ± 0.24 MPa for the best optimized sample. The moisture retention lied between 87%–89%, gel fraction 80%–85%, and water vapor transmission up to 104 ± 1.9g m^(-2)h^(-1)showing great properties of the fabricated membrane. Swelling percentage surged to 225% for blood while porosity fluctuated between 44% ± 2.1% and 52.5% ± 2.3%. Oxygen permeability reached up to 8.02 mg/L showing the breathable nature of fabricated membranes. The nanocomposite membrane shown excellent antibacterial activity for both gram-positive and gram-negative bacteria with a maximum zone of inhibition 30 ± 0.25 mm and 36.23 ± 0.23 mm respectively. Furthermore, nanoparticles maintained sustainable release following non-fickian diffusion. The fabricated membrane demonstrated the application of inorganic filler to enhance the strength of biopolymer hydrogel with superior properties.These results envisage the potential of synthesized membrane to be used as wound dressing, artificial skin and load-bearing scaffolds.
文摘Silver nanoparticles(AgNPs)are of potential interest because of their effective antibacterial and antiviral activities.Capping agents are used for exhibiting a better antibacterial activity than uncapped Ag NPs.There are very few reports that have shown the usage of AgNPs for in-vivo antibacterial therapy.Citrate-capped silver nanoparticles were synthesized chemically by citrate reduction method;the size of Cit-AgNPs was determined by an atomic force microscope(AFM)and was between 15-90 nm.Acinetobacter baumannii(A.baumannii)isolates were the only sensitive species to Cit-AgNPs.MICs and MBC of Cit-AgNPs were determined by using A.baumannii.The results showed an additive effect of Cit-AgNPs.Four mice groups were infected with a sub-lethal dose of A.baumannii intraperitoneally,IP.The single daily dose of Cit-AgNPs and imipenem plus Cit-AgNPs combination were administered IP.Imipenem and phosphate buffer saline(PBS)was used as positive control and negative control,respectively.Interestingly,only the PBS-treated group showed growth of A.baumannii in the liver and spleen of sacrificed mice.Histopathologically,Cit-AgNPs showed antibacterial activity and had an additive effect when combined with imipenem in vivo and in vitro.Moreover,the Cit-AgNPs showed dose-dependent activity and the organs differed in the illumination of the toxicity effect of Cit-AgNPs even after high dose administration.In conclusion,Cit-capped AgNPs had antibacterial activity against multiple drug resistant(MDR)A.baumannii but not against K.pneumoniae and E.coli.Cit-capped AgNPs increased the inhibition zone of imipenem in additive effect;the minimum inhibitory concentration and the minimum bactericidal concentration of Cit-capped AgNPs were relatively low.Cit-capped AgNPs eliminated A.baumannii infection in vivo when it was given alone or in combination with imipenem.The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed in the illumination of the inflammatory effect of Cit-AgNPs after high dose administration.It is not recommended to use Cit-capped AgNPs systemically despite their valuable additive antibacterial effect especially with a high dose and the combination with imipenem,Topical administration needs to be evaluated.
