Recent advance in large-scale genome se-quencing has generated a huge volume of pro-tein sequences. In order to timely utilize the in-formation hidden in these newly discovered sequences, it is highly desired to devel...Recent advance in large-scale genome se-quencing has generated a huge volume of pro-tein sequences. In order to timely utilize the in-formation hidden in these newly discovered sequences, it is highly desired to develop com- putational methods for efficiently identifying their various attributes because the information thus obtained will be very useful for both basic research and drug development. Particularly, it would be even more useful and welcome if a user-friendly web-server could be provided for each of these methods. In this minireview, a sy- stematic introduction is presented to highlight the development of these web-servers by our group during the last three years.展开更多
This article focuses on a profile of personnel who work with retired volunteers. The data was sourced by using two standardised measuring instrument, interviews, focus groups and observation. The Leadership Qualities ...This article focuses on a profile of personnel who work with retired volunteers. The data was sourced by using two standardised measuring instrument, interviews, focus groups and observation. The Leadership Qualities Inventory (LQI-S) was used as one standardised measuring instrument. The following elements were measured namely leadership, people-oriented, task-oriented and self-oriented qualities. The Neethling Brain Instrument (NBI) was used as a second standardised instrument. This instru- ment measured the subconscious’ preferences in four quadrants namely analytical (L1), systematic (L2), emotional (R2) and holistic (R1). From the data an empowerment programme was developed for personnel involved at service centres for elderly persons.展开更多
Oat (Avena sativa L.) and wheat (Triticum aestivum L.) vary in the structure of their inflores-cences and also in how pollination proceeds within the inflorescence. In both species the grain position in the spikelet d...Oat (Avena sativa L.) and wheat (Triticum aestivum L.) vary in the structure of their inflores-cences and also in how pollination proceeds within the inflorescence. In both species the grain position in the spikelet determines grain weight potential. Primary grains in oat and proximal grains in wheat weigh more than secondary and distal grains. This variation in grain weight can potentially result from differences in post-pollination cell division in the grain. In this study pollination duration and dynamics were analyzed from head samples collected at two-day intervals, starting from the pollination of the most advanced floret. The number of grain cells was determined for individual grains throughout the inflorescence, starting from the pollination event. When mature, grain position in the spikelet and spike was noted and grain weight assessed. Pollination advance in oat proceeded from the uppermost primary floret towards the basal spikelets in ten to eleven days. Within the spikelet, the primary floret was pollinated on average one day earlier than the secondary floret. In wheat, pollination duration was four to five days, starting from the proximal florets in the mid-section of the inflorescence progressing towards the apical and basal spikelets. Proximal florets were pollinated one to two days earlier than distal florets. Maximum cell number in primary grains exceeded that of secondary grains in two oat cultivars. Similarly, primary grains were heavier than secondary grains. Cell number and single grain weight were correlated in terms of grain position in the spikelet (primary – secondary) and cultivar. Oat cultivar Belinda had a higher single grain weight than Fiia, which was also expressed as larger grain cell number. In wheat, proximal grains had higher maximum cell numbers and were also heavier than distal grains. This grain weight gradient was apparent throughout the inflorescence. Consequently, grain cell number is one of the possible regulators of grain-filling capacity in both cereal crops.展开更多
Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a disabling condition of unknown cause having multi-system manifestations. Our group has investigated the potential role of transient receptor potential (...Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a disabling condition of unknown cause having multi-system manifestations. Our group has investigated the potential role of transient receptor potential (TRP) ion channels in the etiology and pathomechanism of this illness. Store-operated calcium entry (SOCE) signaling is the primary intracellular calcium signaling mechanism in non-excitable cells and is associated with TRP ion channels. While the sub-family (Canonical) TRPC has been traditionally associated with this important cellular mechanism, a member of the TRPM sub-family group (Melastatin), TRPM3, has also been recently identified as participating in SOCE in white matter of the central nervous system. We have identified single nucleotide polymorphisms (SNPs) in TRP genes in natural killer (NK) cells and peripheral blood mononuclear cells (PBMCs) in CFS/ME patients. We also describe biochemical pathway changes and calcium signaling perturbations in blood cells from patients. The ubiquitous distribution of TRP ion channels and specific locations of sub-family group members such as TRPM3 suggest a contribution to systemic pathology in CFS/ME.展开更多
文摘Recent advance in large-scale genome se-quencing has generated a huge volume of pro-tein sequences. In order to timely utilize the in-formation hidden in these newly discovered sequences, it is highly desired to develop com- putational methods for efficiently identifying their various attributes because the information thus obtained will be very useful for both basic research and drug development. Particularly, it would be even more useful and welcome if a user-friendly web-server could be provided for each of these methods. In this minireview, a sy- stematic introduction is presented to highlight the development of these web-servers by our group during the last three years.
文摘This article focuses on a profile of personnel who work with retired volunteers. The data was sourced by using two standardised measuring instrument, interviews, focus groups and observation. The Leadership Qualities Inventory (LQI-S) was used as one standardised measuring instrument. The following elements were measured namely leadership, people-oriented, task-oriented and self-oriented qualities. The Neethling Brain Instrument (NBI) was used as a second standardised instrument. This instru- ment measured the subconscious’ preferences in four quadrants namely analytical (L1), systematic (L2), emotional (R2) and holistic (R1). From the data an empowerment programme was developed for personnel involved at service centres for elderly persons.
文摘Oat (Avena sativa L.) and wheat (Triticum aestivum L.) vary in the structure of their inflores-cences and also in how pollination proceeds within the inflorescence. In both species the grain position in the spikelet determines grain weight potential. Primary grains in oat and proximal grains in wheat weigh more than secondary and distal grains. This variation in grain weight can potentially result from differences in post-pollination cell division in the grain. In this study pollination duration and dynamics were analyzed from head samples collected at two-day intervals, starting from the pollination of the most advanced floret. The number of grain cells was determined for individual grains throughout the inflorescence, starting from the pollination event. When mature, grain position in the spikelet and spike was noted and grain weight assessed. Pollination advance in oat proceeded from the uppermost primary floret towards the basal spikelets in ten to eleven days. Within the spikelet, the primary floret was pollinated on average one day earlier than the secondary floret. In wheat, pollination duration was four to five days, starting from the proximal florets in the mid-section of the inflorescence progressing towards the apical and basal spikelets. Proximal florets were pollinated one to two days earlier than distal florets. Maximum cell number in primary grains exceeded that of secondary grains in two oat cultivars. Similarly, primary grains were heavier than secondary grains. Cell number and single grain weight were correlated in terms of grain position in the spikelet (primary – secondary) and cultivar. Oat cultivar Belinda had a higher single grain weight than Fiia, which was also expressed as larger grain cell number. In wheat, proximal grains had higher maximum cell numbers and were also heavier than distal grains. This grain weight gradient was apparent throughout the inflorescence. Consequently, grain cell number is one of the possible regulators of grain-filling capacity in both cereal crops.
文摘Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a disabling condition of unknown cause having multi-system manifestations. Our group has investigated the potential role of transient receptor potential (TRP) ion channels in the etiology and pathomechanism of this illness. Store-operated calcium entry (SOCE) signaling is the primary intracellular calcium signaling mechanism in non-excitable cells and is associated with TRP ion channels. While the sub-family (Canonical) TRPC has been traditionally associated with this important cellular mechanism, a member of the TRPM sub-family group (Melastatin), TRPM3, has also been recently identified as participating in SOCE in white matter of the central nervous system. We have identified single nucleotide polymorphisms (SNPs) in TRP genes in natural killer (NK) cells and peripheral blood mononuclear cells (PBMCs) in CFS/ME patients. We also describe biochemical pathway changes and calcium signaling perturbations in blood cells from patients. The ubiquitous distribution of TRP ion channels and specific locations of sub-family group members such as TRPM3 suggest a contribution to systemic pathology in CFS/ME.
