High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the sa...High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.展开更多
短视频时代,随着人们阅读习惯的改变,图书馆阅读推广信息以短视频的形式席卷而来。文章以高校图书馆短视频阅读推广效果为结果导向,阐释了联合技术采纳与使用(Unified Theory of Acceptance and Use of Technology,UTAUT)模型应用于高...短视频时代,随着人们阅读习惯的改变,图书馆阅读推广信息以短视频的形式席卷而来。文章以高校图书馆短视频阅读推广效果为结果导向,阐释了联合技术采纳与使用(Unified Theory of Acceptance and Use of Technology,UTAUT)模型应用于高校图书馆短视频阅读推广的价值,通过调查问卷获取数据,利用SPSS26.0对数据进行处理,分析出影响权重较高的因素,提出高校图书馆短视频阅读推广优化路径。展开更多
文摘High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.
文摘短视频时代,随着人们阅读习惯的改变,图书馆阅读推广信息以短视频的形式席卷而来。文章以高校图书馆短视频阅读推广效果为结果导向,阐释了联合技术采纳与使用(Unified Theory of Acceptance and Use of Technology,UTAUT)模型应用于高校图书馆短视频阅读推广的价值,通过调查问卷获取数据,利用SPSS26.0对数据进行处理,分析出影响权重较高的因素,提出高校图书馆短视频阅读推广优化路径。
