OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood ...OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood mononuclear cell-1(THP-1)derived foam cells were used to examine the possible anti-atherosclerotic effect of XSWD and XSWD-medicated serum.Atherosclerosis-prone apolipoprotein E-deficient(Apo E-/-)mice were utilized for in vivo analysis.After an 8-week high-fat diet(HFD)adminstration,25 male Apo E-/-mice were randomly divided into the model group,different doses of XSWD groups(1.25,2.5,5 mg/m L),and atorvastatin group(2.6 mg/kg).Following a continuous 8-week intervention,all mice underwent examination for AS lesion formation and assessment of its serum lipid profile.To investigate the effect on the gut microbiome,16S r RNA gene sequencing targeting the V3-V4 hypervariable region was performed on the colonic content of mice.RESULTS:XSWD administration attenuated lipid deposition in THP-1 cells,significantly reduced aortic plaque lesions,improved the lipid profile,and normalized GM composition in HFD-fed Apo E-/-mice.CONCLUSION:This study investigated the potential antiatherosclerotic and gut microbio-ta-restoring effects of XSWD in Apo E-/-mice,with findings suggesting that XSWD may be a promising preventive measure against atherosclerosis through its ability to reduce lipid accumula-tion in foam cells,improve lipid profile,and restore gut microbiota composition.展开更多
基金National Natural Science Foundation of China:the AntiAtherosclerotic Mechanism of Xiong’s Shiwei Wendan decoction based on the Regulation of Reverse Cholesterol Transport via the Micro RNA-33A Adenosine Triphosphate-binding Cassette Subfamily A Member 1/Adenosine Triphosphate-binding Cassette Subfamily A Member 1 Pathway(No.81603600)the Natural Science Foundation of Hunan Province of China:the Anti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on the Activation of Lipophagy Mediated by the AMP-activated Protein Kinase/Mechanistic Target of Rapamycin Pathway(No.2021JJ30510)the Health Research Project of Hunan Provincial Health Commission:the An-ti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on Lipophagy Mediated by the Micro RNA-499a-3p/Autophagy-related 5 Pathway(No.D202303018265)。
文摘OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood mononuclear cell-1(THP-1)derived foam cells were used to examine the possible anti-atherosclerotic effect of XSWD and XSWD-medicated serum.Atherosclerosis-prone apolipoprotein E-deficient(Apo E-/-)mice were utilized for in vivo analysis.After an 8-week high-fat diet(HFD)adminstration,25 male Apo E-/-mice were randomly divided into the model group,different doses of XSWD groups(1.25,2.5,5 mg/m L),and atorvastatin group(2.6 mg/kg).Following a continuous 8-week intervention,all mice underwent examination for AS lesion formation and assessment of its serum lipid profile.To investigate the effect on the gut microbiome,16S r RNA gene sequencing targeting the V3-V4 hypervariable region was performed on the colonic content of mice.RESULTS:XSWD administration attenuated lipid deposition in THP-1 cells,significantly reduced aortic plaque lesions,improved the lipid profile,and normalized GM composition in HFD-fed Apo E-/-mice.CONCLUSION:This study investigated the potential antiatherosclerotic and gut microbio-ta-restoring effects of XSWD in Apo E-/-mice,with findings suggesting that XSWD may be a promising preventive measure against atherosclerosis through its ability to reduce lipid accumula-tion in foam cells,improve lipid profile,and restore gut microbiota composition.
