OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood ...OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood mononuclear cell-1(THP-1)derived foam cells were used to examine the possible anti-atherosclerotic effect of XSWD and XSWD-medicated serum.Atherosclerosis-prone apolipoprotein E-deficient(Apo E-/-)mice were utilized for in vivo analysis.After an 8-week high-fat diet(HFD)adminstration,25 male Apo E-/-mice were randomly divided into the model group,different doses of XSWD groups(1.25,2.5,5 mg/m L),and atorvastatin group(2.6 mg/kg).Following a continuous 8-week intervention,all mice underwent examination for AS lesion formation and assessment of its serum lipid profile.To investigate the effect on the gut microbiome,16S r RNA gene sequencing targeting the V3-V4 hypervariable region was performed on the colonic content of mice.RESULTS:XSWD administration attenuated lipid deposition in THP-1 cells,significantly reduced aortic plaque lesions,improved the lipid profile,and normalized GM composition in HFD-fed Apo E-/-mice.CONCLUSION:This study investigated the potential antiatherosclerotic and gut microbio-ta-restoring effects of XSWD in Apo E-/-mice,with findings suggesting that XSWD may be a promising preventive measure against atherosclerosis through its ability to reduce lipid accumula-tion in foam cells,improve lipid profile,and restore gut microbiota composition.展开更多
Objective:To analyze the effect of Shiwei Powder combined with stone composition analysis on stone removal efficiency and complication rates after ureteroscopic holmium laser lithotripsy(FURL).Methods:Ninety six patie...Objective:To analyze the effect of Shiwei Powder combined with stone composition analysis on stone removal efficiency and complication rates after ureteroscopic holmium laser lithotripsy(FURL).Methods:Ninety six patients with FURL admitted to the hospital from March 2023 to March 2025 were selected and randomly divided into two groups.The observation group was treated with FURL combined with Shiwei Powder,while the control group was treated with FURL monotherapy.Based on the stone composition of the two groups,the stone removal efficiency,complication rates,recurrence rates,TCM syndrome scores,and inflammatory factor levels were compared.Results:The stone removal efficiency of the observation group was higher than that of the control group.The stone composition was mainly calcium oxalate monohydrate and composite stones containing calcium oxalate monohydrate.The incidence of postoperative complications was lower in the observation group than in the control group(P<0.05).After 3–6 months of follow-up,the recurrence rate in the observation group was lower than that in the control group(P<0.05).After treatment,the TCM syndrome scores and inflammatory factor levels in the observation group were lower than those in the control group(P<0.05).Conclusion:Based on the results of urinary stone composition analysis,patients treated with Shiwei Powder after FURL have higher stone removal efficiency.The most significant effect is observed in urinary stones composed of calcium oxalate monohydrate and composite stones containing calcium oxalate monohydrate.This treatment can improve stone removal,prevent complications,reduce recurrences,effectively improve disease symptoms,and reduce inflammation.展开更多
有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄...有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄石韦近四十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从有柄石韦中分离出超过100种化合物,包括黄酮类、三萜类、木脂素类、酚类和其他类等。有柄石韦提取物具有显著的生物活性,包括抗氧化、抗菌、抗炎、利尿、促进伤口愈合、抗肾结石、抗糖尿病等。可为有柄石韦药用资源后续深入系统地研究和临床开发提供科学依据和理论参考。展开更多
基金National Natural Science Foundation of China:the AntiAtherosclerotic Mechanism of Xiong’s Shiwei Wendan decoction based on the Regulation of Reverse Cholesterol Transport via the Micro RNA-33A Adenosine Triphosphate-binding Cassette Subfamily A Member 1/Adenosine Triphosphate-binding Cassette Subfamily A Member 1 Pathway(No.81603600)the Natural Science Foundation of Hunan Province of China:the Anti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on the Activation of Lipophagy Mediated by the AMP-activated Protein Kinase/Mechanistic Target of Rapamycin Pathway(No.2021JJ30510)the Health Research Project of Hunan Provincial Health Commission:the An-ti-Atherosclerotic Mechanism of Xiong's Shiwei Wendan Decoction based on Lipophagy Mediated by the Micro RNA-499a-3p/Autophagy-related 5 Pathway(No.D202303018265)。
文摘OBJECTIVE:To evaluate the anti-atherosclerotic potential and gut microbiota(GM)modulation effects of Xiong's Shiwei Wendan decoction(熊氏十味温胆汤,XSWD).METHODS:For in vitro study,Tsuchiya human peripheral blood mononuclear cell-1(THP-1)derived foam cells were used to examine the possible anti-atherosclerotic effect of XSWD and XSWD-medicated serum.Atherosclerosis-prone apolipoprotein E-deficient(Apo E-/-)mice were utilized for in vivo analysis.After an 8-week high-fat diet(HFD)adminstration,25 male Apo E-/-mice were randomly divided into the model group,different doses of XSWD groups(1.25,2.5,5 mg/m L),and atorvastatin group(2.6 mg/kg).Following a continuous 8-week intervention,all mice underwent examination for AS lesion formation and assessment of its serum lipid profile.To investigate the effect on the gut microbiome,16S r RNA gene sequencing targeting the V3-V4 hypervariable region was performed on the colonic content of mice.RESULTS:XSWD administration attenuated lipid deposition in THP-1 cells,significantly reduced aortic plaque lesions,improved the lipid profile,and normalized GM composition in HFD-fed Apo E-/-mice.CONCLUSION:This study investigated the potential antiatherosclerotic and gut microbio-ta-restoring effects of XSWD in Apo E-/-mice,with findings suggesting that XSWD may be a promising preventive measure against atherosclerosis through its ability to reduce lipid accumula-tion in foam cells,improve lipid profile,and restore gut microbiota composition.
文摘Objective:To analyze the effect of Shiwei Powder combined with stone composition analysis on stone removal efficiency and complication rates after ureteroscopic holmium laser lithotripsy(FURL).Methods:Ninety six patients with FURL admitted to the hospital from March 2023 to March 2025 were selected and randomly divided into two groups.The observation group was treated with FURL combined with Shiwei Powder,while the control group was treated with FURL monotherapy.Based on the stone composition of the two groups,the stone removal efficiency,complication rates,recurrence rates,TCM syndrome scores,and inflammatory factor levels were compared.Results:The stone removal efficiency of the observation group was higher than that of the control group.The stone composition was mainly calcium oxalate monohydrate and composite stones containing calcium oxalate monohydrate.The incidence of postoperative complications was lower in the observation group than in the control group(P<0.05).After 3–6 months of follow-up,the recurrence rate in the observation group was lower than that in the control group(P<0.05).After treatment,the TCM syndrome scores and inflammatory factor levels in the observation group were lower than those in the control group(P<0.05).Conclusion:Based on the results of urinary stone composition analysis,patients treated with Shiwei Powder after FURL have higher stone removal efficiency.The most significant effect is observed in urinary stones composed of calcium oxalate monohydrate and composite stones containing calcium oxalate monohydrate.This treatment can improve stone removal,prevent complications,reduce recurrences,effectively improve disease symptoms,and reduce inflammation.
文摘有柄石韦(Pyrrosia petiolosa)是水龙骨科石韦属植物,也是一种常用的中药材,被广泛用于治疗肾盂肾炎、慢性支气管炎、尿路结石等病症。通过检索中国知网、PubMed、Web of Science等数据库,查找国内外已经发表的有柄石韦相关文献,对有柄石韦近四十年的化学成分和生物活性研究进行系统地梳理、归纳,发现目前已从有柄石韦中分离出超过100种化合物,包括黄酮类、三萜类、木脂素类、酚类和其他类等。有柄石韦提取物具有显著的生物活性,包括抗氧化、抗菌、抗炎、利尿、促进伤口愈合、抗肾结石、抗糖尿病等。可为有柄石韦药用资源后续深入系统地研究和临床开发提供科学依据和理论参考。
