Microglia,the resident immune cells in the central nervous system(CNS),rapidly transition from a resting to an active state in the acute phase of ischemic brain injury.This active state mediates a pro-inflammatory res...Microglia,the resident immune cells in the central nervous system(CNS),rapidly transition from a resting to an active state in the acute phase of ischemic brain injury.This active state mediates a pro-inflammatory response that can exacerbate the injury.Targeting the pro-inflammatory response of microglia in the semi-dark band during this acute phase may effectively reduce brain injury.Shionone(SH),an active ingredient extracted from the dried roots and rhizomes of the genus Aster(Asteraceae),has been reported to regulate the inflammatory response of macrophages in sepsis-induced acute lung injury.However,its function in post-stroke neuroinflammation,particularly microglia-mediated neuroinflammation,remains uninvestigated.This study found that SH significantly inhibited lipopolysaccharide(LPS)-induced elevation of inflammatory cytokines,including interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and inducible nitric oxide synthase(iNOS),in microglia in vitro.Furthermore,the results demonstrated that SH alleviated infarct volume and improved behavioral performance in middle cerebral artery occlusion(MCAO)mice,which may be attributed to the inhibition of the microglial inflammatory response induced by SH treatment.Mechanistically,SH potently inhibited the phosphorylation of serine-threonine protein kinase B(AKT),mammalian target of rapamycin(mTOR),and signal transducer and activator of transcription 3(STAT3).These findings suggest that SH may be a potential therapeutic agent for relieving ischemic stroke(IS)by alleviating microglia-associated neuroinflammation.展开更多
Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by...Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by HPLC on a RP18 column(150 mm×3.9 mm,5μm)with a mobile phase composed of acetonitrile-0.05%phosphoric acid water(98:2)at a flow rate of 1.0 mL/min.UV Detector was set at 200 nm and friedelin was chosen as an internal standard.Results The linear range of the standard curves was(0.3443-22.0)μg/mL with the correlation coefficient of 0.9968.The intra-and inter-day precisions were all below 10%and the relative error was-3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study.After ig administration of RAPE,T1/2(ka)is(33.09±7.32)min and T1/2(ke)is(84.95±22.34)min.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81920108017 and 82130036)the Key Research and Development Program of Jiangsu Province of China(No.BE2020620)Jiangsu Province Key Medical Discipline(No.ZDXKA2016020)。
文摘Microglia,the resident immune cells in the central nervous system(CNS),rapidly transition from a resting to an active state in the acute phase of ischemic brain injury.This active state mediates a pro-inflammatory response that can exacerbate the injury.Targeting the pro-inflammatory response of microglia in the semi-dark band during this acute phase may effectively reduce brain injury.Shionone(SH),an active ingredient extracted from the dried roots and rhizomes of the genus Aster(Asteraceae),has been reported to regulate the inflammatory response of macrophages in sepsis-induced acute lung injury.However,its function in post-stroke neuroinflammation,particularly microglia-mediated neuroinflammation,remains uninvestigated.This study found that SH significantly inhibited lipopolysaccharide(LPS)-induced elevation of inflammatory cytokines,including interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and inducible nitric oxide synthase(iNOS),in microglia in vitro.Furthermore,the results demonstrated that SH alleviated infarct volume and improved behavioral performance in middle cerebral artery occlusion(MCAO)mice,which may be attributed to the inhibition of the microglial inflammatory response induced by SH treatment.Mechanistically,SH potently inhibited the phosphorylation of serine-threonine protein kinase B(AKT),mammalian target of rapamycin(mTOR),and signal transducer and activator of transcription 3(STAT3).These findings suggest that SH may be a potential therapeutic agent for relieving ischemic stroke(IS)by alleviating microglia-associated neuroinflammation.
基金support from the scientific and technological project of Hebei Province,China(No 09276423D)
文摘Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by HPLC on a RP18 column(150 mm×3.9 mm,5μm)with a mobile phase composed of acetonitrile-0.05%phosphoric acid water(98:2)at a flow rate of 1.0 mL/min.UV Detector was set at 200 nm and friedelin was chosen as an internal standard.Results The linear range of the standard curves was(0.3443-22.0)μg/mL with the correlation coefficient of 0.9968.The intra-and inter-day precisions were all below 10%and the relative error was-3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study.After ig administration of RAPE,T1/2(ka)is(33.09±7.32)min and T1/2(ke)is(84.95±22.34)min.
