“Shen”is one of the core contents in the basic theory of traditional Chinese medicine(TCM).Taoist philosophy plays an important role in promoting the theoretical shaping and medical transformation of“Shen”.Taking ...“Shen”is one of the core contents in the basic theory of traditional Chinese medicine(TCM).Taoist philosophy plays an important role in promoting the theoretical shaping and medical transformation of“Shen”.Taking the medical history as the thread and medical philosophy as the speculative dimension,this paper systematically sorts out the evolutionary trajectory of“Shen”in TCM from supernatural worship to philosophical abstraction,and then to medical embodiment,deeply analyzes the shaping effect of Taoist thought on the theoretical framework of“Shen”,and interprets its contemporary medical value.The research shows that Taoist philosophy provides a cosmological foundation,the core of the interdependence of essence,Qi,blood and Shen,and the guidance of spiritual inward concentration for“Shen”in TCM;Huangdi Neijing(Yellow Emperor’s Internal Classic)deeply integrates Taoist philosophy with medical practice,promoting the medical transformation of“Shen”.Its core ideas of“the unity of nature and man”and“the unity of form and Shen”provide traditional wisdom for modern psychosomatic medicine and systems medicine.The“Shen”theory,for which Taoist philosophy serves as an important intellectual source,is not only a crucial foundation for the inheritance and innovation of TCM theory,but also provides important ideological reference for the holistic development of modern medicine.展开更多
Shennong (approximately lived between 3000_(BCE) and 2000_(BCE)),also known as the Flame Emperor, is widely regarded as the pioneer of ancient Chinese agricultural civilization and pharmacology. He holds a highly este...Shennong (approximately lived between 3000_(BCE) and 2000_(BCE)),also known as the Flame Emperor, is widely regarded as the pioneer of ancient Chinese agricultural civilization and pharmacology. He holds a highly esteemed position in traditional culture, and Shennong's Herbals (Shen Nong Ben Cao Jing), which is attributed to him, is the earliest known pharmacological monograph in China.In the origin myths of Chinese civilization, Shennong's image is a blend of historical reality and mythological legend.展开更多
Chronic glomerulonephritis is a renal disease characterized by multifactorial etiology,intricate pathogenesis,and persistent inflammatory response involving macrophages as the principal inflammatory cells.The polariza...Chronic glomerulonephritis is a renal disease characterized by multifactorial etiology,intricate pathogenesis,and persistent inflammatory response involving macrophages as the principal inflammatory cells.The polarization of macrophages into anti-inflammatory and pro-inflammatory phenotypes necessitates a delicate balance in chronic glomerulonephritis,mirroring the concept of yin-yang equilibrium in Chinese medicine theory pertaining to kidney function.Clinical studies have demonstrated the efficacy of Jin Gui Shen Qi Pills in the treatment of chronic glomerulonephritis,while pharmacological studies have revealed that the formula theory of Jin Gui Shen Qi Pills can modulate macrophage polarization and enhance the equilibrium between anti-inflammatory and pro-inflammatory activities.This article examines the correlation between the formula theory of Jin Gui Shen Qi Pills and the concepts of yin and yang by utilizing theoretical analysis,as well as incorporating findings from contemporary clinical and experimental studies.The study aims to elucidate the connection between the pathogenesis of chronic glomerulonephritis and the polarization of macrophages,ultimately proposing that Jin Gui Shen Qi Pills can potentially regulate macrophage polarization as a treatment for chronic glomerulonephritis.The proposition is made that Jin Gui Shen Qi Pills plays a regulatory role in macrophage polarization for the treatment of chronic glomerulonephritis,aiming to establish equilibrium between anti-inflammatory and pro-inflammatory responses,thereby offering a novel approach for integrating Chinese and Western medicine in the clinical management of chronic glomerulonephritis.展开更多
Objective: This study aims to explore the application effects of Shen-Shi Zhi-Tong plaster combined with Lei-Huo moxibustion in preventing and treating gastrointestinal reactions induced by chemotherapy, particularly ...Objective: This study aims to explore the application effects of Shen-Shi Zhi-Tong plaster combined with Lei-Huo moxibustion in preventing and treating gastrointestinal reactions induced by chemotherapy, particularly its efficacy in managing chemotherapy-induced nausea and vomiting (CINV). Methods: A total of 100 patients with gastrointestinal reactions who were hospitalized in the oncology department of Nanning Third People’s Hospital from January to December 2023 were selected as study subjects. They were randomly divided into two groups: the observation group (n = 50) received standard antiemetic treatment in conjunction with Shen-Shi Zhi-Tong plaster and Lei-Huo moxibustion, while the control group (n = 50) received only standard antiemetic treatment and Lei-Huo moxibustion. The occurrence of nausea and vomiting during chemotherapy was recorded using a CINV nursing log, and the incidence rates of acute and delayed vomiting were statistically analyzed. Results: The incidence rate of acute vomiting in the observation group was 42%, significantly lower than the control group’s 64% (P = 0.0300);the incidence rate of delayed vomiting was 36% in the observation group compared to 48% in the control group (P = 0.1900). The mean nausea grade in the observation group was 1.08, significantly lower than 2.02 in the control group (P Conclusion: Shen-Shi Zhi-Tong plaster combined with Lei-Huo moxibustion can effectively reduce the incidence of nausea and vomiting in patients undergoing highly emetogenic chemotherapy and shows good clinical application prospects. This study provides strong support for the adoption of such combined therapies in the prevention and treatment of CINV in the future.展开更多
Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen pro...Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen producing marrow”, the composite recipe, Yisui Shenxueling Granule (YSSXL), consisting of Chinese drugs for nourishing Shen and supplementing marrow (NS&SM) was given orally to 7 8 patients with β thalassemia (49 of the severe type and 29 of moderate type ), 3 times a day, 10 g each time (for children, the dose would be reduced proper ly), with 3 months as one therapeutic course, and no blood transfusion used in t he course. The clinical therapeutic efficacy and hematologic parameters in patie nts were observed, and systemic gene analysis was conducted with PAGE, PCR, PCR SSCP, RT PCR and DNA sequences analysis and mRNA detection, in order to s tudy the molecular mechanism from the relationships between genetic mutation and clinical efficacy, gene expression and its regulation. Results: YSSXL showed obvious therapeutic effect in treating β thalassemia. Gene analysis revealed that it did not change the genetic mutatio n type, but could obviously increase hemoglobin, fetal hemoglobin (HbF), γ/(β+ γ) globin ratio, γ globin mRNA expression and GM CSF mRNA expression in patients, as well as the GM CSFmRMA in marrow of mice after 60 Co radia tion. Conclusion: YSSXL has a remarkable therapeutic effect on β tha lassemia, and its possible mechanism is its action in unlocking γ gene, in creasing the γ globin expression and enhancing HbF synthesis so as to compe nsate for the gene defect. This study has opened a new path for the treatment of β thalassemia with TCM.展开更多
The earliest Ben Cao Jing(Classics of Materia Medica;25 C.E.–220 C.E.)appeared during the reign of the Han Dynasty(202 B.C.E.–220 C.E.);their origins are traceable to the religious group called Fang Xian Dao.In addi...The earliest Ben Cao Jing(Classics of Materia Medica;25 C.E.–220 C.E.)appeared during the reign of the Han Dynasty(202 B.C.E.–220 C.E.);their origins are traceable to the religious group called Fang Xian Dao.In addition to their use as a medical reference,the Ben Cao Jing works were also used for religious purposes.Tao Hongjing(456 C.E.–536 C.E.),a famous doctor and Daoist(one member of the Chinese local religion,Daoism)of the Liang Dynasty(502 C.E.–557 C.E.),compiled the Ben Cao Jing Ji Zhu(Variorum of Classics of Materia Medica;456 C.E.–536 C.E.)based on the Shen Nong Ben Cao Jing(Shennong’s Classic of Materia Medica;25 C.E.–220 C.E.).During the compilation of this book,Tao made a radical medical revision to the Shen Nong Ben Cao Jing,by reducing its religious contents while complementing its medical contents.After such revisions,the ancient Ben Cao Jing was transformed from a religious manual into an herbal pharmacopoeia.展开更多
Objective:To probe into the intervening action of polysaccharides of Zhu Zi Shen(Rhizoma Panacis Majoris)(PZZS) on oxidative stress and hemodynamics in rats with adriamycin-induced chronic congestive heart failure(CHF...Objective:To probe into the intervening action of polysaccharides of Zhu Zi Shen(Rhizoma Panacis Majoris)(PZZS) on oxidative stress and hemodynamics in rats with adriamycin-induced chronic congestive heart failure(CHF).Methods:After SD rats were successfully modeled with adriamycin,they were randomly divided into a normal control group,a model group,a PZZS group,and a captopril group,and were administrated respectively.At the end of experiment,the hemodynamic function,whole heart weight index,and the blood CK,SOD,MDA,NO,NOS were detected;and the myocardial morphological examinations were carried out.Results:Compared with the normal control group,the arterial systolic pressure(SBP),diastolic pressure(DBP),mean arterial pressure(MAP),heart rate(HR),left ventricular systolic peak(LVSP),and left ventricular pressure change rate(dp/dtmax) significantly decreased,and left ventricular end diastolic pressure(LVEDP),whole heart weight index,the blood CK,MDA,NO,NOS significantly increased in the model group.PZZS significantly improved the hemodynamic function,lowered the MDA and NO levels,and decreased the CK and NOS activities in the CHF rats.Conclusion:PZZS can improve the hemodynamic function,and alleviate the oxidative stress reaction in the CHF rat.展开更多
Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the cur...Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the current study,we investigated the role of Aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo.Subsequently,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown.The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells.The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1.AQP1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of E-cadherin.The protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells.These results revealed that AQP1 knockdown promoted EMT.Furthermore,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells.In sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.展开更多
BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyr...BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyrrhizinate.In this study,we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment.AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase(ERK)phosphorylation.METHODS Common bile duct-ligated rats were used for in vivo experiments.Hepatic stellate cells-T6(HSC-T6)cells were used for in vitro experiments.Hematoxylin and eosin staining and Masson staining,biochemical assays,hydroxyproline(Hyp)assays,enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis,liver function,the inflammatory response and ERK phosphorylation.The CCK8 assay,immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells.RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile ductligated rats after 3 wk of treatment,as demonstrated by histological changes,hydroxyproline assays and collagen I concentrations.GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α)and interlukin-1β]and NF-κB.In addition,GSFF decreased ERK phosphorylation.In vitro,GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factorβ1(TGF-β1)stimulation and decreased the synthesis of collagen I.GSFF had the greatest effect at a concentration of 0.5μmol/L.GSFF inhibited the expression ofα-smooth muscle actin(α-SMA),a marker of HSC activation,in HSC-T6 cells.Consistent with the in vivo results,GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB.CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro.These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.展开更多
This study aimed to profile the chemical constituents of Zi-Shen pill(ZSP) and its metabolites in plasma, urine, and prostate tissue, after administration into rats. Based on the chromatographic retention behavior, fr...This study aimed to profile the chemical constituents of Zi-Shen pill(ZSP) and its metabolites in plasma, urine, and prostate tissue, after administration into rats. Based on the chromatographic retention behavior, fragmentation patterns of chemical components, published literatures, and literature databases, an UPLC-Q-TOF/MS(LC-TOF/MS) method was established to identify the components of ZSP and its metabolites in biological samples. A total of 101 compounds were identified and tentatively characterized from the ZSP, including alkaloids, xanthones, and timosaponins. Except for 33 prototype components, 22 metabolites were detected in the plasma, urine, and prostate, and mainly came from Phellodendri Amurensis Cortex and Anemarrhenae Rhizoma. It was found that glucuronidation and sulfation were the major metabolic processes of xanthones, while oxidation, demethylation, and glucuronidation were the major metabolic pathways of alkaloids. In summary, the present study provided important chemical information on the metabolism of ZSP, indicating that alkaloids might be able to be absorbed into the prostate. The results provided a basis for further studies of the mechanisms of action for ZSP.展开更多
Coal mining subsidence is a universal environmental-geological problem in mining areas. By selecting the Shen-Dong coal mining subsidence area as the research field, this paper studies the changes in precipitation inf...Coal mining subsidence is a universal environmental-geological problem in mining areas. By selecting the Shen-Dong coal mining subsidence area as the research field, this paper studies the changes in precipitation infiltration recharge in the circumstances of coal mining subsidence by means of field geological investigation and laboratory simulation experiments, which is expected to provide a scientific basis for eco-environmental restoration in the mining area. The results indicate that at the unstable stage of subsidence, three types of subsidence in the Shen-Dong mining area have positive effects on the precipitation infiltration recharge, and the type of full-thickness bedrock subsidence has the greatest influence. In the stable stage of subsidence, the precipitation infiltration process after long- term drought and the moisture migration in the aeration zone undergo three different stages： evaporation-infiltration before precipitation, infiltration-upward infiltration-infiltration during precipitation and evaporation-infiltration after precipitation. During a heavy rainfall infiltration process, the wetting front movement in fine sand, coarse sand and dualistic structure of fine-coarse sand consists of two stages： the stage of wetting front movement during precipitation, in which the wetting front movement distance has linear relationship with the depth, and the stage of wetting front movement after precipitation, in which the wetting front movement distance has the power function relationship with the depth. The wetting front movement velocity is influenced by the rainfall amount and the lithology in the aeration zone. However, as the depth increases, the movement velocity will decay exponentially.展开更多
Shock-timing experiments are indispensable to inertial confinement fusion mainly because the timing of multiple shock waves is crucial to understanding the processes of laser irradiation of targets. Investigations int...Shock-timing experiments are indispensable to inertial confinement fusion mainly because the timing of multiple shock waves is crucial to understanding the processes of laser irradiation of targets. Investigations into shock waves driven by a two-step radiation pulse in polystyrene（CH） capsule targets are experimentally conducted at the Shen Guang Ⅱ laser facility. Differing from the traditional shock-timing implementation in which one shock wave could catch up with another one in solid CH, in this experiment, the second shock front in a rarefaction CH layer is observed through velocity interferometry. This second shock could also be made to converge with rarefaction waves within only a few micrometers of the CH capsule by designing the two-shock coalescence time. A shock-timing diagnostic technique to tune the multi-shock convergence in the CH capsule can thereby be achieved.The experimental results in the CH layer are quasi-quantitatively interpreted using streamlines simulated with the Multi-1 D program. The experimental results are expected to offer important information for target structure and laser pulse design, both of which are important for realizing inertial confinement fusion.展开更多
The effect and underlying mechanism of Bu-Shen-An-Tai recipe on ovarian apoptosis in mice with controlled ovarian hyperstimulation(COH) implantation dysfunction were studied. The COH implantation dysfunction model in ...The effect and underlying mechanism of Bu-Shen-An-Tai recipe on ovarian apoptosis in mice with controlled ovarian hyperstimulation(COH) implantation dysfunction were studied. The COH implantation dysfunction model in mice was established by intraperitoneal injection of 7.5 IU pregnant mare’s serum gonadotrophin(PMSG), followed by 7.5 IU human chorionic gonadotrophin(HCG) 48 h later. Then the female mice were mated with male at a ratio of 2:1 in the same cage at 6:00 p.m. The female mice from normal group were injected intraperitoneally with normal saline and mated at the corresponding time. Day 1 of pregnancy was recorded by examining its vaginal smears at 8:00 a.m. of the next day. Fifty successfully pregnant mice were equally randomly divided into 5 groups: normal control pregnant group(NC), COH implantation dysfunction model group(COH), low dosage of Bu-Shen-An-Tai recipe group(LOW), middle dosage of Bu-Shen-An-Tai recipe group(MID) and high dosage of Bu-Shen-An-Tai recipe group(HIGH). Then from day 1, the mice in different groups were respectively intragastrically given corresponding treatments at 9:00 a.m. for 5 consecutive days. The concentrations of 17β-estradiol(E) and progesterone(P) were determined by radioimmunoassay(RIA). The ultrastructural changes of ovarian tissues were observed by transmission electron microscope(TEM). The histopathological changes of ovarian tissues were observed by HE staining. The number of atretic follicles and pregnant corpus luteum were also recorded. TUNEL was applied to measure apoptotic cells of ovarian tissues. Western blotting was used to detect the protein expression of apoptosis-related factors like Bax, Bcl-2 and cleaved-caspase-3 in ovarian tissue of mice. The results showed that ovarian weight, the concentrations of Eand P, the number of atretic follicles and pregnant corpus luteum, as well as the apoptosis of granulosa cells were significantly increased in the COH group. The ultrastructures of ovarian tissues in the COH group showed that chromatin in granulosa cells was increased, agglutinated, aggregated or crescent-shaped. The focal cavitation and the typical apoptotic bodies could be seen in granulosa cells in the late stage of apoptosis. After the treatment with different doses of Bu-Shen-An-Tai recipe, the ultrastructural changes of ovarian granulosa cells apoptosis were dramatically improved and even disappeared under TEM. Visible mitochondria and mitochondrial cristae were increased and vacuoles were significantly reduced. The lipid dropltes were shown in a circluar or oval shape. The protein expression levels of Bax and cleaved-caspase-3 were decreased, and the expression of Bcl-2 protein was increased after treatment. It was concluded that Bu-Shen-An-Tai recipe can inhibit the apoptosis of ovarian granulosa cells, probably by up-regulating the protein expression of Bcl-2 and down-regulating Bax and cleaved-caspase-3, which contributes to the formation and maintenance of ovarian corpus luteum. It’s helpful to promote the embryonic implantation, to reduce embryo loss and ultimately to improve the success rate of pregnancy.展开更多
基金supported by the Tianjin Municipal Education Commission Scientific Research Plan Project(Grant No.2022ZD042).
文摘“Shen”is one of the core contents in the basic theory of traditional Chinese medicine(TCM).Taoist philosophy plays an important role in promoting the theoretical shaping and medical transformation of“Shen”.Taking the medical history as the thread and medical philosophy as the speculative dimension,this paper systematically sorts out the evolutionary trajectory of“Shen”in TCM from supernatural worship to philosophical abstraction,and then to medical embodiment,deeply analyzes the shaping effect of Taoist thought on the theoretical framework of“Shen”,and interprets its contemporary medical value.The research shows that Taoist philosophy provides a cosmological foundation,the core of the interdependence of essence,Qi,blood and Shen,and the guidance of spiritual inward concentration for“Shen”in TCM;Huangdi Neijing(Yellow Emperor’s Internal Classic)deeply integrates Taoist philosophy with medical practice,promoting the medical transformation of“Shen”.Its core ideas of“the unity of nature and man”and“the unity of form and Shen”provide traditional wisdom for modern psychosomatic medicine and systems medicine.The“Shen”theory,for which Taoist philosophy serves as an important intellectual source,is not only a crucial foundation for the inheritance and innovation of TCM theory,but also provides important ideological reference for the holistic development of modern medicine.
文摘Shennong (approximately lived between 3000_(BCE) and 2000_(BCE)),also known as the Flame Emperor, is widely regarded as the pioneer of ancient Chinese agricultural civilization and pharmacology. He holds a highly esteemed position in traditional culture, and Shennong's Herbals (Shen Nong Ben Cao Jing), which is attributed to him, is the earliest known pharmacological monograph in China.In the origin myths of Chinese civilization, Shennong's image is a blend of historical reality and mythological legend.
基金This work was supported by the National Natural Science Foundation of China[81774071 to M.G.].
文摘Chronic glomerulonephritis is a renal disease characterized by multifactorial etiology,intricate pathogenesis,and persistent inflammatory response involving macrophages as the principal inflammatory cells.The polarization of macrophages into anti-inflammatory and pro-inflammatory phenotypes necessitates a delicate balance in chronic glomerulonephritis,mirroring the concept of yin-yang equilibrium in Chinese medicine theory pertaining to kidney function.Clinical studies have demonstrated the efficacy of Jin Gui Shen Qi Pills in the treatment of chronic glomerulonephritis,while pharmacological studies have revealed that the formula theory of Jin Gui Shen Qi Pills can modulate macrophage polarization and enhance the equilibrium between anti-inflammatory and pro-inflammatory activities.This article examines the correlation between the formula theory of Jin Gui Shen Qi Pills and the concepts of yin and yang by utilizing theoretical analysis,as well as incorporating findings from contemporary clinical and experimental studies.The study aims to elucidate the connection between the pathogenesis of chronic glomerulonephritis and the polarization of macrophages,ultimately proposing that Jin Gui Shen Qi Pills can potentially regulate macrophage polarization as a treatment for chronic glomerulonephritis.The proposition is made that Jin Gui Shen Qi Pills plays a regulatory role in macrophage polarization for the treatment of chronic glomerulonephritis,aiming to establish equilibrium between anti-inflammatory and pro-inflammatory responses,thereby offering a novel approach for integrating Chinese and Western medicine in the clinical management of chronic glomerulonephritis.
文摘Objective: This study aims to explore the application effects of Shen-Shi Zhi-Tong plaster combined with Lei-Huo moxibustion in preventing and treating gastrointestinal reactions induced by chemotherapy, particularly its efficacy in managing chemotherapy-induced nausea and vomiting (CINV). Methods: A total of 100 patients with gastrointestinal reactions who were hospitalized in the oncology department of Nanning Third People’s Hospital from January to December 2023 were selected as study subjects. They were randomly divided into two groups: the observation group (n = 50) received standard antiemetic treatment in conjunction with Shen-Shi Zhi-Tong plaster and Lei-Huo moxibustion, while the control group (n = 50) received only standard antiemetic treatment and Lei-Huo moxibustion. The occurrence of nausea and vomiting during chemotherapy was recorded using a CINV nursing log, and the incidence rates of acute and delayed vomiting were statistically analyzed. Results: The incidence rate of acute vomiting in the observation group was 42%, significantly lower than the control group’s 64% (P = 0.0300);the incidence rate of delayed vomiting was 36% in the observation group compared to 48% in the control group (P = 0.1900). The mean nausea grade in the observation group was 1.08, significantly lower than 2.02 in the control group (P Conclusion: Shen-Shi Zhi-Tong plaster combined with Lei-Huo moxibustion can effectively reduce the incidence of nausea and vomiting in patients undergoing highly emetogenic chemotherapy and shows good clinical application prospects. This study provides strong support for the adoption of such combined therapies in the prevention and treatment of CINV in the future.
基金This item was supported by National Funds of Natural Sciences (No. 3017119 9) and Natural Science Foundation of Guangxi Autonomous Region (No. 014402C)
文摘Objective: To explore the possibility of using traditional Chinese medicine (TCM) in treating β thalassemia, its clinical effect and molecular mechanism of the action.Methods: According to the TCM theory of“Shen producing marrow”, the composite recipe, Yisui Shenxueling Granule (YSSXL), consisting of Chinese drugs for nourishing Shen and supplementing marrow (NS&SM) was given orally to 7 8 patients with β thalassemia (49 of the severe type and 29 of moderate type ), 3 times a day, 10 g each time (for children, the dose would be reduced proper ly), with 3 months as one therapeutic course, and no blood transfusion used in t he course. The clinical therapeutic efficacy and hematologic parameters in patie nts were observed, and systemic gene analysis was conducted with PAGE, PCR, PCR SSCP, RT PCR and DNA sequences analysis and mRNA detection, in order to s tudy the molecular mechanism from the relationships between genetic mutation and clinical efficacy, gene expression and its regulation. Results: YSSXL showed obvious therapeutic effect in treating β thalassemia. Gene analysis revealed that it did not change the genetic mutatio n type, but could obviously increase hemoglobin, fetal hemoglobin (HbF), γ/(β+ γ) globin ratio, γ globin mRNA expression and GM CSF mRNA expression in patients, as well as the GM CSFmRMA in marrow of mice after 60 Co radia tion. Conclusion: YSSXL has a remarkable therapeutic effect on β tha lassemia, and its possible mechanism is its action in unlocking γ gene, in creasing the γ globin expression and enhancing HbF synthesis so as to compe nsate for the gene defect. This study has opened a new path for the treatment of β thalassemia with TCM.
文摘The earliest Ben Cao Jing(Classics of Materia Medica;25 C.E.–220 C.E.)appeared during the reign of the Han Dynasty(202 B.C.E.–220 C.E.);their origins are traceable to the religious group called Fang Xian Dao.In addition to their use as a medical reference,the Ben Cao Jing works were also used for religious purposes.Tao Hongjing(456 C.E.–536 C.E.),a famous doctor and Daoist(one member of the Chinese local religion,Daoism)of the Liang Dynasty(502 C.E.–557 C.E.),compiled the Ben Cao Jing Ji Zhu(Variorum of Classics of Materia Medica;456 C.E.–536 C.E.)based on the Shen Nong Ben Cao Jing(Shennong’s Classic of Materia Medica;25 C.E.–220 C.E.).During the compilation of this book,Tao made a radical medical revision to the Shen Nong Ben Cao Jing,by reducing its religious contents while complementing its medical contents.After such revisions,the ancient Ben Cao Jing was transformed from a religious manual into an herbal pharmacopoeia.
文摘Objective:To probe into the intervening action of polysaccharides of Zhu Zi Shen(Rhizoma Panacis Majoris)(PZZS) on oxidative stress and hemodynamics in rats with adriamycin-induced chronic congestive heart failure(CHF).Methods:After SD rats were successfully modeled with adriamycin,they were randomly divided into a normal control group,a model group,a PZZS group,and a captopril group,and were administrated respectively.At the end of experiment,the hemodynamic function,whole heart weight index,and the blood CK,SOD,MDA,NO,NOS were detected;and the myocardial morphological examinations were carried out.Results:Compared with the normal control group,the arterial systolic pressure(SBP),diastolic pressure(DBP),mean arterial pressure(MAP),heart rate(HR),left ventricular systolic peak(LVSP),and left ventricular pressure change rate(dp/dtmax) significantly decreased,and left ventricular end diastolic pressure(LVEDP),whole heart weight index,the blood CK,MDA,NO,NOS significantly increased in the model group.PZZS significantly improved the hemodynamic function,lowered the MDA and NO levels,and decreased the CK and NOS activities in the CHF rats.Conclusion:PZZS can improve the hemodynamic function,and alleviate the oxidative stress reaction in the CHF rat.
基金the National Natural Science Foundation of China(Nos.81673839 and 82074304)the Young Talents Foundation of Zhejiang Traditional Chinese Medicine Science and Technology Program(No.2019ZQ014)the Science Foundation of Zhejiang Chinese Medical University(No.2020ZZ13).
文摘Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the current study,we investigated the role of Aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo.Subsequently,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown.The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells.The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1.AQP1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of E-cadherin.The protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells.These results revealed that AQP1 knockdown promoted EMT.Furthermore,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells.In sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1.
基金Supported by the Innovation Team of the Beijing University of Chinese Medicine,No.2019-JYB-TD-006the National Natural Science Foundation of China,No.81873099Scientific Research Support Plan for the Construction of Doctoral Program of University of Tibetan Medicine.
文摘BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyrrhizinate.In this study,we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment.AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase(ERK)phosphorylation.METHODS Common bile duct-ligated rats were used for in vivo experiments.Hepatic stellate cells-T6(HSC-T6)cells were used for in vitro experiments.Hematoxylin and eosin staining and Masson staining,biochemical assays,hydroxyproline(Hyp)assays,enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis,liver function,the inflammatory response and ERK phosphorylation.The CCK8 assay,immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells.RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile ductligated rats after 3 wk of treatment,as demonstrated by histological changes,hydroxyproline assays and collagen I concentrations.GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α)and interlukin-1β]and NF-κB.In addition,GSFF decreased ERK phosphorylation.In vitro,GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factorβ1(TGF-β1)stimulation and decreased the synthesis of collagen I.GSFF had the greatest effect at a concentration of 0.5μmol/L.GSFF inhibited the expression ofα-smooth muscle actin(α-SMA),a marker of HSC activation,in HSC-T6 cells.Consistent with the in vivo results,GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB.CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro.These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.
基金supported by the National Natural Science Foundation of China(No.81325024)
文摘This study aimed to profile the chemical constituents of Zi-Shen pill(ZSP) and its metabolites in plasma, urine, and prostate tissue, after administration into rats. Based on the chromatographic retention behavior, fragmentation patterns of chemical components, published literatures, and literature databases, an UPLC-Q-TOF/MS(LC-TOF/MS) method was established to identify the components of ZSP and its metabolites in biological samples. A total of 101 compounds were identified and tentatively characterized from the ZSP, including alkaloids, xanthones, and timosaponins. Except for 33 prototype components, 22 metabolites were detected in the plasma, urine, and prostate, and mainly came from Phellodendri Amurensis Cortex and Anemarrhenae Rhizoma. It was found that glucuronidation and sulfation were the major metabolic processes of xanthones, while oxidation, demethylation, and glucuronidation were the major metabolic pathways of alkaloids. In summary, the present study provided important chemical information on the metabolism of ZSP, indicating that alkaloids might be able to be absorbed into the prostate. The results provided a basis for further studies of the mechanisms of action for ZSP.
基金supported by the National Natural Science Foundation of China(No.41130637, No.40472124)International Cooperation Projects of Ministry of Science and Technology(2005DFA90200)
文摘Coal mining subsidence is a universal environmental-geological problem in mining areas. By selecting the Shen-Dong coal mining subsidence area as the research field, this paper studies the changes in precipitation infiltration recharge in the circumstances of coal mining subsidence by means of field geological investigation and laboratory simulation experiments, which is expected to provide a scientific basis for eco-environmental restoration in the mining area. The results indicate that at the unstable stage of subsidence, three types of subsidence in the Shen-Dong mining area have positive effects on the precipitation infiltration recharge, and the type of full-thickness bedrock subsidence has the greatest influence. In the stable stage of subsidence, the precipitation infiltration process after long- term drought and the moisture migration in the aeration zone undergo three different stages： evaporation-infiltration before precipitation, infiltration-upward infiltration-infiltration during precipitation and evaporation-infiltration after precipitation. During a heavy rainfall infiltration process, the wetting front movement in fine sand, coarse sand and dualistic structure of fine-coarse sand consists of two stages： the stage of wetting front movement during precipitation, in which the wetting front movement distance has linear relationship with the depth, and the stage of wetting front movement after precipitation, in which the wetting front movement distance has the power function relationship with the depth. The wetting front movement velocity is influenced by the rainfall amount and the lithology in the aeration zone. However, as the depth increases, the movement velocity will decay exponentially.
基金Supported by the Science and Technology on Plasma Physics Laboratory under Grant No 9140C6801021001
文摘Shock-timing experiments are indispensable to inertial confinement fusion mainly because the timing of multiple shock waves is crucial to understanding the processes of laser irradiation of targets. Investigations into shock waves driven by a two-step radiation pulse in polystyrene（CH） capsule targets are experimentally conducted at the Shen Guang Ⅱ laser facility. Differing from the traditional shock-timing implementation in which one shock wave could catch up with another one in solid CH, in this experiment, the second shock front in a rarefaction CH layer is observed through velocity interferometry. This second shock could also be made to converge with rarefaction waves within only a few micrometers of the CH capsule by designing the two-shock coalescence time. A shock-timing diagnostic technique to tune the multi-shock convergence in the CH capsule can thereby be achieved.The experimental results in the CH layer are quasi-quantitatively interpreted using streamlines simulated with the Multi-1 D program. The experimental results are expected to offer important information for target structure and laser pulse design, both of which are important for realizing inertial confinement fusion.
基金supported by the National Natural Sciences Foundation of China(No.81473494)
文摘The effect and underlying mechanism of Bu-Shen-An-Tai recipe on ovarian apoptosis in mice with controlled ovarian hyperstimulation(COH) implantation dysfunction were studied. The COH implantation dysfunction model in mice was established by intraperitoneal injection of 7.5 IU pregnant mare’s serum gonadotrophin(PMSG), followed by 7.5 IU human chorionic gonadotrophin(HCG) 48 h later. Then the female mice were mated with male at a ratio of 2:1 in the same cage at 6:00 p.m. The female mice from normal group were injected intraperitoneally with normal saline and mated at the corresponding time. Day 1 of pregnancy was recorded by examining its vaginal smears at 8:00 a.m. of the next day. Fifty successfully pregnant mice were equally randomly divided into 5 groups: normal control pregnant group(NC), COH implantation dysfunction model group(COH), low dosage of Bu-Shen-An-Tai recipe group(LOW), middle dosage of Bu-Shen-An-Tai recipe group(MID) and high dosage of Bu-Shen-An-Tai recipe group(HIGH). Then from day 1, the mice in different groups were respectively intragastrically given corresponding treatments at 9:00 a.m. for 5 consecutive days. The concentrations of 17β-estradiol(E) and progesterone(P) were determined by radioimmunoassay(RIA). The ultrastructural changes of ovarian tissues were observed by transmission electron microscope(TEM). The histopathological changes of ovarian tissues were observed by HE staining. The number of atretic follicles and pregnant corpus luteum were also recorded. TUNEL was applied to measure apoptotic cells of ovarian tissues. Western blotting was used to detect the protein expression of apoptosis-related factors like Bax, Bcl-2 and cleaved-caspase-3 in ovarian tissue of mice. The results showed that ovarian weight, the concentrations of Eand P, the number of atretic follicles and pregnant corpus luteum, as well as the apoptosis of granulosa cells were significantly increased in the COH group. The ultrastructures of ovarian tissues in the COH group showed that chromatin in granulosa cells was increased, agglutinated, aggregated or crescent-shaped. The focal cavitation and the typical apoptotic bodies could be seen in granulosa cells in the late stage of apoptosis. After the treatment with different doses of Bu-Shen-An-Tai recipe, the ultrastructural changes of ovarian granulosa cells apoptosis were dramatically improved and even disappeared under TEM. Visible mitochondria and mitochondrial cristae were increased and vacuoles were significantly reduced. The lipid dropltes were shown in a circluar or oval shape. The protein expression levels of Bax and cleaved-caspase-3 were decreased, and the expression of Bcl-2 protein was increased after treatment. It was concluded that Bu-Shen-An-Tai recipe can inhibit the apoptosis of ovarian granulosa cells, probably by up-regulating the protein expression of Bcl-2 and down-regulating Bax and cleaved-caspase-3, which contributes to the formation and maintenance of ovarian corpus luteum. It’s helpful to promote the embryonic implantation, to reduce embryo loss and ultimately to improve the success rate of pregnancy.
