Background:In this research,we explored the operational principles of Huangqin Shegan decoction(HQSGD)for addressing acute pneumonia utilizing network pharmacology(NP)and transcriptomic analysis.Methods:Methods:A rat ...Background:In this research,we explored the operational principles of Huangqin Shegan decoction(HQSGD)for addressing acute pneumonia utilizing network pharmacology(NP)and transcriptomic analysis.Methods:Methods:A rat model of acute pneumonia was developed by treating rats with lipopolysaccharide(LPS)through a non-exposed tracheal drip.The pharmacological effects of HQSGD were evaluated via histopathological analysis of rat lung tissues,histological scoring of lung injury,assessment of lung index,serum inflammatory factors,oxidative stress levels,western blotting,and qRT-PCR.The active compounds of HQSGD were detected utilizing ultra-performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS).NP and transcriptomic analysis were integrated to determine signaling pathways implicated in the pharmacological activity of HQSGD.The expression levels of mRNA and protein for factors implicated in these pathways were evaluated in rat lung tissues via qRT-PCR and western blotting,respectively.Results:HQSGD alleviated acute pneumonia in rats by reducing the lung index and the levels of TNF-α,IL-1β,CRP,and MDA while increasing the levels of SOD.The UPLC-MS/MS and NP techniques facilitated the identification of 28 bioactive constituents present in HQSGD.The principal 20 KEGG pathways were identified by intersecting the targets of HQSGD with pneumonia-related targets.These pathways were screened by comparing the transcriptomic data of the blank and model cohorts and those of the model and drug administration cohorts.GO and KEGG analyses indicated that the PI3K/AKT/NF-κB pathway was a potentially effective target of HQSGD.Conclusion:This investigation revealed the overall multi-component,multi-target,and multi-pathway interactions of HQSGD in the treatment of acute pneumonia.展开更多
Objective: to apply the combined therapy of shegan mahuang decoction and acupoint application in the treatment of acute exacerbation of bronchial asthma in children. Methods: the children with acute exacerbation of br...Objective: to apply the combined therapy of shegan mahuang decoction and acupoint application in the treatment of acute exacerbation of bronchial asthma in children. Methods: the children with acute exacerbation of bronchial asthma who were treated in our hospital from January 2021 to January 2022 were selected as the research subjects, and the effects of different treatments were compared, including shegan mahuang decoction and acupoint application + shegan mahuang decoction. Results: after the effective treatment plan was implemented, the condition improved, especially the children treated with acupoint application + shegan mahuang decoction had a higher total effective rate, a lower incidence of adverse reactions, a good trend in FVC and other levels, and symptoms and signs. The scores were lower, and the children who received acupoint application treatment were relatively poor, and the difference was significant (P<0.05). Conclusion: in the treatment of acute exacerbation of bronchial asthma in children, shegan mahuang decoction combined with acupoint application has obvious application value and high safety, and plays a positive role in further shortening the course of the disease in children.展开更多
目的采用网络药理学和动物实验探讨射干止咳胶囊治疗感染后咳嗽的分子机制。方法通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)获得射干的活性成分,利...目的采用网络药理学和动物实验探讨射干止咳胶囊治疗感染后咳嗽的分子机制。方法通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)获得射干的活性成分,利用Swiss Target Prediction获取射干的相关靶点,并于GeneCards、OMIM、DisGeNET数据库筛选出的疾病靶点取交集获得射干止咳胶囊治疗感染后咳嗽的潜在靶点;采用STRING和Cytoscape 3.9.0构建“药物-有效成分-交集靶点”网络并筛选关键靶点;然后进行基因本体(Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析;Western blot法检测肺组织叉头状转录因子3(forkhead box protein,Foxp3)、维甲酸相关孤儿核受体γt(retinoic-acid-related orphan nuclear receptor gamma t,RORγt)蛋白表达。结果经过筛选,射干止咳胶囊中的木犀草素、野鸢尾黄素、异鼠李素、鸢尾甲黄素A、鸢尾甲黄素B、鸢尾黄素等多个关键活性成分,可能通过作用在蛋白质激酶Bα(protein kinase Bα,AKT1)、肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素-6(interleukin-6,IL-6)、血管内皮生长因子A(vascular endothelial growth factor A,VEGFA)等核心基因表达的蛋白上参与感染后咳嗽的治疗;KEGG富集分析所涉及的通路包括FoxO信号通路(FoxO signaling pathway)、IL-17信号通路(IL-17 signaling pathway)、Th17细胞分化(Th17 cell differentiation)、Th1和Th2细胞分化(Th1 and Th2 cell differentiation)、B细胞受体信号通路等;动物实验验证射干止咳胶囊可影响Foxp3、RORγt蛋白表达。结论射干止咳胶囊可以影响Foxp3、RORγt蛋白表达,调控Treg/Th17平衡通路发挥其抗炎作用,有效治疗感染后咳嗽。展开更多
In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） method to simultaneously determine 11 compounds in two hospital preparations （Shegan mixture ...In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） method to simultaneously determine 11 compounds in two hospital preparations （Shegan mixture and Gandi capsules）, including ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin. The chromatographic separation was conducted on ZORBAX SB-C18 （2.1 mm×50 mm, 1.8 μm）. Eleven analytes and IS were detected using ESI and MRM combined with positive and negative scanning switch. Caffeic acid, ferulic acid, scutellarin and belamcandin were detected using negative ion mode detection, whereas ephedrine, rutin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin were detected using positive ion mode detection. The quantification limits of ephedrine, caffeic acid, scutellarin, belamcandin, baicalin, baicalein, irisfiorentin and wogonin were 4.90×10^-3 ng/mL, 7.80 ng/mL, 6.8 ng/mL, 5.3×10^-2 ng/mL, 4.20×10^-3 ng/mL, 4.6×10^-2 ng/mL, 1.44×10^-4 ng/mL, 4.85 ng/mL, 0.23 ng/mL, 3.18× 10^-4 ng/mL and 2.95× 10^-4 ng/mL, respectively. The detection limits of these components were 2.90× 10^-4 ng/mL, 0.77 ng/mL, 2.0 ng/mL, 0.016 ng/mL, 1.3×10^-3 ng/mL, 3.33×10^-4 ng/mL, 4.32×10^-5 ng/mL, 1.46 ng/mL, 0.07 ng/mL, 9.5×10^-5 ng/mL and 8.84× 10^-5 ng/mL, respectively. All calibration curves showed good linearity （R^2〉0.99） within the test range. The RSD values of intra-day and inter-day precision were less than 5%, and the average recovery rates of the 11 components ranged fi＇om 80% to 120%. In conclusion, our newly developed method was simple, rapid, sensitive and accurate. It could be used to determine ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin in Shegan mixture and Gandi capsules, which was helpful for the quality control of drugs.展开更多
基金the"Qin Medicine"Quality Evaluation and Resource Development Discipline Innovation Team Project of Shaanxi University of Traditional Chinese Medicine(2019-QN01)Shaanxi University of Traditional Chinese Medicine School of Pharmacy/Shaanxi Engineering Research Centre for the Application and Development of Qinling Herbal Medicine,"Qin Medicine"Research and Development Key Laboratory(2019-QYPT-002)+2 种基金Shaanxi Provincial Administration of Traditional Chinese Medicine Province,Research and Development Key Laboratory(2019-QYPT-002)Shaanxi Provincial Administration of Traditional Chinese Medicine Province Chinese medicine province-wide earmarked special project:"Qin medicine planting and breeding guide research"(2021-QYZL-02)Shaanxi Provincial Science and Technology Department project:Chinese medicine Scutellaria baicalensis germplasm selection,seedling breeding and planting key technology research(2016KTTSSF01-01-01)and other projects.
文摘Background:In this research,we explored the operational principles of Huangqin Shegan decoction(HQSGD)for addressing acute pneumonia utilizing network pharmacology(NP)and transcriptomic analysis.Methods:Methods:A rat model of acute pneumonia was developed by treating rats with lipopolysaccharide(LPS)through a non-exposed tracheal drip.The pharmacological effects of HQSGD were evaluated via histopathological analysis of rat lung tissues,histological scoring of lung injury,assessment of lung index,serum inflammatory factors,oxidative stress levels,western blotting,and qRT-PCR.The active compounds of HQSGD were detected utilizing ultra-performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS).NP and transcriptomic analysis were integrated to determine signaling pathways implicated in the pharmacological activity of HQSGD.The expression levels of mRNA and protein for factors implicated in these pathways were evaluated in rat lung tissues via qRT-PCR and western blotting,respectively.Results:HQSGD alleviated acute pneumonia in rats by reducing the lung index and the levels of TNF-α,IL-1β,CRP,and MDA while increasing the levels of SOD.The UPLC-MS/MS and NP techniques facilitated the identification of 28 bioactive constituents present in HQSGD.The principal 20 KEGG pathways were identified by intersecting the targets of HQSGD with pneumonia-related targets.These pathways were screened by comparing the transcriptomic data of the blank and model cohorts and those of the model and drug administration cohorts.GO and KEGG analyses indicated that the PI3K/AKT/NF-κB pathway was a potentially effective target of HQSGD.Conclusion:This investigation revealed the overall multi-component,multi-target,and multi-pathway interactions of HQSGD in the treatment of acute pneumonia.
文摘Objective: to apply the combined therapy of shegan mahuang decoction and acupoint application in the treatment of acute exacerbation of bronchial asthma in children. Methods: the children with acute exacerbation of bronchial asthma who were treated in our hospital from January 2021 to January 2022 were selected as the research subjects, and the effects of different treatments were compared, including shegan mahuang decoction and acupoint application + shegan mahuang decoction. Results: after the effective treatment plan was implemented, the condition improved, especially the children treated with acupoint application + shegan mahuang decoction had a higher total effective rate, a lower incidence of adverse reactions, a good trend in FVC and other levels, and symptoms and signs. The scores were lower, and the children who received acupoint application treatment were relatively poor, and the difference was significant (P<0.05). Conclusion: in the treatment of acute exacerbation of bronchial asthma in children, shegan mahuang decoction combined with acupoint application has obvious application value and high safety, and plays a positive role in further shortening the course of the disease in children.
基金Shanghai Science and Technology Commission Research Fund(Grant No.11DZ1972500)Shanghai Health Bureau of Traditional Chinese Medicine Research Fund(Grant No.2012G003A)Shanghai Municipal Education Commission of Outstanding Young Teachers in Special Fund(Grant No.ZZjdyx13092)
文摘In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） method to simultaneously determine 11 compounds in two hospital preparations （Shegan mixture and Gandi capsules）, including ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin. The chromatographic separation was conducted on ZORBAX SB-C18 （2.1 mm×50 mm, 1.8 μm）. Eleven analytes and IS were detected using ESI and MRM combined with positive and negative scanning switch. Caffeic acid, ferulic acid, scutellarin and belamcandin were detected using negative ion mode detection, whereas ephedrine, rutin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin were detected using positive ion mode detection. The quantification limits of ephedrine, caffeic acid, scutellarin, belamcandin, baicalin, baicalein, irisfiorentin and wogonin were 4.90×10^-3 ng/mL, 7.80 ng/mL, 6.8 ng/mL, 5.3×10^-2 ng/mL, 4.20×10^-3 ng/mL, 4.6×10^-2 ng/mL, 1.44×10^-4 ng/mL, 4.85 ng/mL, 0.23 ng/mL, 3.18× 10^-4 ng/mL and 2.95× 10^-4 ng/mL, respectively. The detection limits of these components were 2.90× 10^-4 ng/mL, 0.77 ng/mL, 2.0 ng/mL, 0.016 ng/mL, 1.3×10^-3 ng/mL, 3.33×10^-4 ng/mL, 4.32×10^-5 ng/mL, 1.46 ng/mL, 0.07 ng/mL, 9.5×10^-5 ng/mL and 8.84× 10^-5 ng/mL, respectively. All calibration curves showed good linearity （R^2〉0.99） within the test range. The RSD values of intra-day and inter-day precision were less than 5%, and the average recovery rates of the 11 components ranged fi＇om 80% to 120%. In conclusion, our newly developed method was simple, rapid, sensitive and accurate. It could be used to determine ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin in Shegan mixture and Gandi capsules, which was helpful for the quality control of drugs.
