OBJECTIVE:To evaluate the protective effect of Zhizi Huangqi Shanzha formula(栀子黄芪山楂方,ZHSF)on aflatoxin-induced liver injury.METHODS:The protective effect of ZHSF on the aflatoxin-induced liver injury was evalua...OBJECTIVE:To evaluate the protective effect of Zhizi Huangqi Shanzha formula(栀子黄芪山楂方,ZHSF)on aflatoxin-induced liver injury.METHODS:The protective effect of ZHSF on the aflatoxin-induced liver injury was evaluated by histological observation,blood cell analysis,evaluation of liver function and immunity,and gut microbiota analysis.RESULTS:ZHSF can significantly up-regulate the percentage of lymphocytes and eosinophils in the blood of Aflatoxin B1-intoxicated mice,down-regulate the levels of serum aspartate aminotransferase,alanine aminotransferase,and malondialdehyde,and recover the liver tissue structure.Aflatoxin poisoning induces a variation of the intestinal flora of mice,and ZHSF may recover the variation of intestinal flora induced by Aflatoxin B1.Cluster analysis showed that the intestinal flora of mice in the intervention group was more similar to that of the control group.Correlation analysis showed that Lachnospiraceae,Desulfovibrio,and Lactobacillus may be the key flora for the pharmacological effects of ZHSF.CONCLUSIONS:ZHSF may protect against aflatoxininduced liver damage,improve immunity,and inhibit oxidative stress by regulating the composition and relative abundance of intestinal flora,which makes it a promising liver-protective candidate drug.展开更多
Objective:To establish the quality standard of Compound Shanzha Granules.Methods:Crataegi Fructus and Cassiae Semen were identified by TLC.The contents of Autrantio-obtusin and Chrysophanol in Cassiae Semen were deter...Objective:To establish the quality standard of Compound Shanzha Granules.Methods:Crataegi Fructus and Cassiae Semen were identified by TLC.The contents of Autrantio-obtusin and Chrysophanol in Cassiae Semen were determined by HPLC.Results:The relevant spots on TLC plates were clear by the blank reference.The contents showed good linearitis in the range of 0.0357~0.3570μg for Autrantio-obtusin and 0.0520~0.5200μg for Chrysophanol.Conclusions:The method are simple,accurate and reproducible.展开更多
目的研究山楂荷叶饮对高脂血症大鼠的作用并探讨其降脂的潜在活性成分及作用靶点。方法建立高脂血症大鼠模型,从血脂水平、体质量、肝脏系数、脂肪质量以及肝脏病理形态变化等方面评价山楂荷叶饮的降脂效果;采用超高效液相色谱-四极杆...目的研究山楂荷叶饮对高脂血症大鼠的作用并探讨其降脂的潜在活性成分及作用靶点。方法建立高脂血症大鼠模型,从血脂水平、体质量、肝脏系数、脂肪质量以及肝脏病理形态变化等方面评价山楂荷叶饮的降脂效果;采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry,UHPLC-QTOF-MS)对山楂荷叶饮中的化学成分进行鉴定,筛选山楂荷叶饮中活性成分与高脂血症的交集靶点,采用STRING数据库对蛋白相互作用网络进行构建与分析,利用Auto Dock Tools软件对活性成分与关键靶点进行分子对接验证。结果山楂荷叶饮能显著降低高脂血症大鼠总胆固醇(total cholesterol,TC)、三酰甘油(triglycerides,TG)和低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)水平,降低体质量及肝脏指数、附睾脂肪和肾周脂肪质量,改善肝脏组织病理形态、红色脂滴沉积情况。从山楂荷叶饮中共检测鉴定出88个化合物,其中生物碱类27个、黄酮类33个、有机酸类19个、其他类9个。网络药理学分析发现,山楂荷叶饮中金丝桃苷、荷叶碱、槲皮素、乌药碱等10个关键成分可能通过调控10个核心靶点发挥对高脂血症的调节作用。分子对接结果显示,关键成分与核心靶点蛋白结合活性良好。结论山楂荷叶饮可通过改善血脂水平、减少肝脏脂肪堆积等发挥对高脂血症的治疗作用,为山楂荷叶饮降血脂成分和机制的研究及产品的开发提供参考。展开更多
目的探讨山楂制剂调脂与肠道菌群的相关性。方法将100只SPF级载脂蛋白E基因敲除小鼠(C57BL/6J Apoe^(-/-)小鼠)随机分为5组,每组20只。分为空白对照组、动脉粥样硬化(atherosclerosis,AS)模型组、AS加抗生素对照组及AS加山楂加抗生素组...目的探讨山楂制剂调脂与肠道菌群的相关性。方法将100只SPF级载脂蛋白E基因敲除小鼠(C57BL/6J Apoe^(-/-)小鼠)随机分为5组,每组20只。分为空白对照组、动脉粥样硬化(atherosclerosis,AS)模型组、AS加抗生素对照组及AS加山楂加抗生素组、AS加山楂组。检测指标:检测小鼠血脂水平,包括低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C);运用RT-PCR技术定量检测腹腔巨噬细胞清道夫受体A1(scavenger receptor class A type 1,SR-A1)、CD36mRNA的表达水平;运用Western-blot方法检测CD36,SR-A1的蛋白表达。结果比较空白对照组、AS模型组、AS加抗生素对照组及AS加山楂加抗生素组、AS加山楂组的血脂水平、CD36和SR-A1的mRNA水平和蛋白水平变化,结果表明:(1)通过与空白对照组比较,AS加山楂组有显著降脂效果;(2)通过与AS模型组比较,AS加抗生素对照组和AS加山楂加抗生素组及AS加山楂组均有降脂效果;(3)AS加山楂加抗生素组降脂水平高于AS加抗生素对照组;(4)AS加山楂降脂水平高于AS加山楂加抗生素组。结论山楂具有改善高胆碱饮食喂养造成动脉粥样硬化模型小鼠血脂的作用,山楂制剂调脂与肠道菌群相关。展开更多
基金the Provincial Natural Science Foundation of Anhui Province-Quantitative Constitutive Effect and Mechanism of Action of Dendrobium Polysaccharides against Tumours based on the Tumour Microenvironment(2208085QH273)Natural Science Research Project for Anhui Universities-mechanism of Anti-bacterial Diarrhea Effect of Probiotic-fermented Portulaca Oleracea in Targeting and Regulating Amino acid Metabolism of Intestinal Flora and Screening of Anti-tumor Active Fraction of Paris polyphylla and its Effect on the Tumour Immune Microenvironment(2023AH052634,2022AH051668)。
文摘OBJECTIVE:To evaluate the protective effect of Zhizi Huangqi Shanzha formula(栀子黄芪山楂方,ZHSF)on aflatoxin-induced liver injury.METHODS:The protective effect of ZHSF on the aflatoxin-induced liver injury was evaluated by histological observation,blood cell analysis,evaluation of liver function and immunity,and gut microbiota analysis.RESULTS:ZHSF can significantly up-regulate the percentage of lymphocytes and eosinophils in the blood of Aflatoxin B1-intoxicated mice,down-regulate the levels of serum aspartate aminotransferase,alanine aminotransferase,and malondialdehyde,and recover the liver tissue structure.Aflatoxin poisoning induces a variation of the intestinal flora of mice,and ZHSF may recover the variation of intestinal flora induced by Aflatoxin B1.Cluster analysis showed that the intestinal flora of mice in the intervention group was more similar to that of the control group.Correlation analysis showed that Lachnospiraceae,Desulfovibrio,and Lactobacillus may be the key flora for the pharmacological effects of ZHSF.CONCLUSIONS:ZHSF may protect against aflatoxininduced liver damage,improve immunity,and inhibit oxidative stress by regulating the composition and relative abundance of intestinal flora,which makes it a promising liver-protective candidate drug.
文摘Objective:To establish the quality standard of Compound Shanzha Granules.Methods:Crataegi Fructus and Cassiae Semen were identified by TLC.The contents of Autrantio-obtusin and Chrysophanol in Cassiae Semen were determined by HPLC.Results:The relevant spots on TLC plates were clear by the blank reference.The contents showed good linearitis in the range of 0.0357~0.3570μg for Autrantio-obtusin and 0.0520~0.5200μg for Chrysophanol.Conclusions:The method are simple,accurate and reproducible.
文摘目的研究山楂荷叶饮对高脂血症大鼠的作用并探讨其降脂的潜在活性成分及作用靶点。方法建立高脂血症大鼠模型,从血脂水平、体质量、肝脏系数、脂肪质量以及肝脏病理形态变化等方面评价山楂荷叶饮的降脂效果;采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry,UHPLC-QTOF-MS)对山楂荷叶饮中的化学成分进行鉴定,筛选山楂荷叶饮中活性成分与高脂血症的交集靶点,采用STRING数据库对蛋白相互作用网络进行构建与分析,利用Auto Dock Tools软件对活性成分与关键靶点进行分子对接验证。结果山楂荷叶饮能显著降低高脂血症大鼠总胆固醇(total cholesterol,TC)、三酰甘油(triglycerides,TG)和低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)水平,降低体质量及肝脏指数、附睾脂肪和肾周脂肪质量,改善肝脏组织病理形态、红色脂滴沉积情况。从山楂荷叶饮中共检测鉴定出88个化合物,其中生物碱类27个、黄酮类33个、有机酸类19个、其他类9个。网络药理学分析发现,山楂荷叶饮中金丝桃苷、荷叶碱、槲皮素、乌药碱等10个关键成分可能通过调控10个核心靶点发挥对高脂血症的调节作用。分子对接结果显示,关键成分与核心靶点蛋白结合活性良好。结论山楂荷叶饮可通过改善血脂水平、减少肝脏脂肪堆积等发挥对高脂血症的治疗作用,为山楂荷叶饮降血脂成分和机制的研究及产品的开发提供参考。
文摘目的探讨山楂制剂调脂与肠道菌群的相关性。方法将100只SPF级载脂蛋白E基因敲除小鼠(C57BL/6J Apoe^(-/-)小鼠)随机分为5组,每组20只。分为空白对照组、动脉粥样硬化(atherosclerosis,AS)模型组、AS加抗生素对照组及AS加山楂加抗生素组、AS加山楂组。检测指标:检测小鼠血脂水平,包括低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C);运用RT-PCR技术定量检测腹腔巨噬细胞清道夫受体A1(scavenger receptor class A type 1,SR-A1)、CD36mRNA的表达水平;运用Western-blot方法检测CD36,SR-A1的蛋白表达。结果比较空白对照组、AS模型组、AS加抗生素对照组及AS加山楂加抗生素组、AS加山楂组的血脂水平、CD36和SR-A1的mRNA水平和蛋白水平变化,结果表明:(1)通过与空白对照组比较,AS加山楂组有显著降脂效果;(2)通过与AS模型组比较,AS加抗生素对照组和AS加山楂加抗生素组及AS加山楂组均有降脂效果;(3)AS加山楂加抗生素组降脂水平高于AS加抗生素对照组;(4)AS加山楂降脂水平高于AS加山楂加抗生素组。结论山楂具有改善高胆碱饮食喂养造成动脉粥样硬化模型小鼠血脂的作用,山楂制剂调脂与肠道菌群相关。
