Aquaculture is one of the most efficient modes of animal protein production and plays an important role in global food security.Aquaculture animals exhibit extraordinarily diverse sexual phenotypes and underlying mech...Aquaculture is one of the most efficient modes of animal protein production and plays an important role in global food security.Aquaculture animals exhibit extraordinarily diverse sexual phenotypes and underlying mechanisms,providing an ideal system to perform sex determination research,one of the important areas in life science.Moreover,sex is also one of the most valuable traits because sexual dimorphism in growth,size,and other economic characteristics commonly exist in aquaculture animals.Here,we synthesize current knowledge of sex determination mechanisms,sex chromosome evolution,reproduction strategies,and sexual dimorphism,and also review several approaches for sex control in aquaculture animals,including artificial gynogenesis,application of sex-specific or sex chromosome-linked markers,artificial sex reversal,as well as gene editing.We anticipate that better understanding of sex determination mechanisms and innovation of sex control approaches will facilitate sustainable development of aquaculture.展开更多
The Cynoglossus semilaevis(half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China.It has a female heterogametic sex determination system(ZW/ZZ) and environmental...The Cynoglossus semilaevis(half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China.It has a female heterogametic sex determination system(ZW/ZZ) and environmental factors can induce sex-reversal of females to phenotypic males,suggesting that it is a promising model for the study of sex determination mechanisms.Additionally,females grow much faster than males and it is feasible to improve the aquaculture production through sex control techniques.This paper reviews the progress in research on sex determination mechanisms research in our laboratory.We have completed whole-genome sequencing and revealed the genome organization and sex chromosome evolution of C.semilaevis.A putative male determining gene dmrt1 was identified and DNA methylation was verified as having a crucial role in the sex reversal process.Genetic maps and sex-specific biomarkers have been used in a marker-assisted selection breeding program and for differentiation of the fish sex.Development and improvement of sex control technologies,including artificial gynogenesis and production of breeding fry with high proportion of females,is also reviewed.These research advances have provided insight into the regulation of sex determination and enabled efficient sex management in artificial culturing of C.semilaevis.展开更多
[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at t...[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at the density of 26 m×26 m（one trap/676 m2） to carry out field control test.[Result] The amount of egg massed reduced by 62.16% after trapping by sex pheromone under the density,and the larvae population was equivalent to those in the conventional control field with pesticides,the control effect was continuous,stable and significant.The larger the control area was,the better the effect was.[Conclusion] Using sex pheromone could effectively control S.litura and reduce pesticide usage for over 80% with economic,safe and efficient features,which should be incorporated into the technical control system of S.litura.展开更多
Because the male silkworm is economically superior to the female in sericulture,male-silkworm rearing through sex control is an interesting question. In order to rearmale silkworms only, a balanced lethal system was e...Because the male silkworm is economically superior to the female in sericulture,male-silkworm rearing through sex control is an interesting question. In order to rearmale silkworms only, a balanced lethal system was established in Japan and former U.S.S.R.by utilizing the recessive female sex-linked gene. Tajima et al. eliminated femaleindividuals by means of mechanical or manual methods according to the sex-limitedegg color or sex-limited larva marking. In addition, Xu An-ying et al. tried to ob-tain only male individuals by inducing androgenesis. However, the above methodscannot yet be utilized in sericulture production because of their harmful influenceson the vitality of silkworm larvas or the hatching rate.展开更多
We studied the trapping effects on Ostrinia furnacalis from the aspects of different hanging heights and different color traps. The results suggested that the hanging height of 1.5 - 2.0 m received a better trapping e...We studied the trapping effects on Ostrinia furnacalis from the aspects of different hanging heights and different color traps. The results suggested that the hanging height of 1.5 - 2.0 m received a better trapping effect, but trap color had little impact on trapping effect. The sex pheromone traps at different densities were placed in corn field. The results showed that sex pheromone lure could trap adult O. furnacalis, and it was feasible to apply sex pheromone in short term fore- cast. Meanwhile, it reduced the number of larvae and eggs, thereby reducing the damage of O. furnacalis. The lure number of 10 per hm2 was more appropriate, which could result in increasing yield of 8.61% compared with control展开更多
This study was conducted to explore and seek high-efficiency and safe green control techniques on sugarcane borers,promote green control of diseases and pests of sugarcane and build a resource-conserving,environmental...This study was conducted to explore and seek high-efficiency and safe green control techniques on sugarcane borers,promote green control of diseases and pests of sugarcane and build a resource-conserving,environmentally friendly and sustainable governance system of diseases and pests of sugarcane.A new sex pheromone trap of sugarcane borers,abamectin· Bacillus thuringiensis (Bt) and tebufenozide were selected and applied in field trials.The use of the new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 0.05% abamectin·10 billion active gemmae/g B.thuringiensis (Bt) WP (1.8 kg/hm 2) or new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 200 g/L tebufenozide SC (1.5 L/hm 2) were the best in the sugarcane production.New sex pheromone trap of sugarcane borers should be installed in early march,and biological agents were mixed with water 900 kg per hectare and sprayed evenly sugarcane plants at the beginning of April.Their control effects of dead heart rate and bored stalk rate could be more than 69.98% and 49.09%,respectively,which were superior to that of the control pesticide 3.6% bisultap GR (90 kg/hm 2).The results showed that new sex pheromone trap of sugarcane borers in combination with abamectin· B.thuringiensis (Bt) or tebufenozide was the optimum mode of green control techniques on sugarcane borers.When it was alternately or coordinately used with other technology,it could delay the emergence and development of drug resistance,and it was worthy of widespread popularization and application in sugarcane area.展开更多
Background:Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion.To derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspr...Background:Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion.To derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspring sex ratio is approximately 50%.At present,artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring.However,flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy goats.Results:In this study,a novel,simple goat sperm sexing technology that activates the toll-like receptor 7/8(TLR7/8),thereby inhibiting X-sperm motility,was investigated.Our results showed that the TLR7/8 coding goat Xchromosome was expressed in approximately 50%of round spermatids in the testis and sperm,as measured from cross-sections of the epididymis and ejaculate,respectively.Importantly,TLR7/8 was located at the tail of the Xsperm.Upon TLR7/8 activation,phosphorylated forms of glycogen synthase kinaseα/β(GSK3α/β)and nuclear factor kappa-B(NF-κB)were detected in the X-sperm,causing reduced mitochondrial activity,ATP levels,and sperm motility.High-motility Y-sperm segregated to the upper layer and the low-motility X-sperm,to the lower layer.Following in vitro fertilisation using the TLR7/8-activated sperm from the lower layer,80.52±6.75%of the embryos were XX females.The TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response;nine embryos were collected from the uterus of two does that conceived.Eight of these were XX embryos,and one was an XY embryo.Conclusions:Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3α/β-hexokinase pathway;this technique could be used to facilitate the efficient production of sexed dairy goat embryos.展开更多
Out of 100 cases of intractable schizophrenia,57 cases were in the group ofComputer-Controlled Electric Acupuncture (CCEA) and 43 in the control group.The therapeutic re-sults showed that clinically CCEA had a remarka...Out of 100 cases of intractable schizophrenia,57 cases were in the group ofComputer-Controlled Electric Acupuncture (CCEA) and 43 in the control group.The therapeutic re-sults showed that clinically CCEA had a remarkably effective rate of 40. 35% on refractoryschizophrenia,which had much better curative effects than those in the control group.Through dy-namic evaluation of brief psychiatric rating scale (BPRS),negative symptom scale (SANS) and posi-tive symptom scale (SAPS) for both groups before,during and after treatment,the results indicatedthat in CCEA group the scores of BPRS,SANS and SAPS after treatment were much less than thosebefore treatment,which were remarkably different from those in the control group (P【0.01).Lab-oratory examination of thyroxin and androgen of patients in both groups before and after treatmentfound a significant result.展开更多
2022年5月以来,全球110多个既往非流行区国家和地区暴发猴痘疫情,通过男男性行为人群(Men who have sex with men,MSM)性接触及其聚集性活动传播扩散。2023年6月2日我国发现首例本土猴痘病例,截至11月30日累计发现1610例,通过开展监测...2022年5月以来,全球110多个既往非流行区国家和地区暴发猴痘疫情,通过男男性行为人群(Men who have sex with men,MSM)性接触及其聚集性活动传播扩散。2023年6月2日我国发现首例本土猴痘病例,截至11月30日累计发现1610例,通过开展监测、病例及其密切接触者追踪调查管理、重点人群健康教育干预等监测防控措施,10月份以来境内猴痘疫情已降至较低水平,但仍持续面临境外输入及境内低水平传播风险。为达到终止猴痘暴发及阻断人传人疫情的防控目标,需进一步加强病例发现、传播链调查、潜在暴露者识别、重点人群干预等工作,持续引导重点人群行为改变、多渠道提升监测发现能力。展开更多
基金supported by the Key Program of Frontier Sciences of the Chinese Academy of Sciences(QYZDY-SSWSMC025)the Strategic Priority Research Program of Chinese Academy of Sciences(XDB31000000)+5 种基金the National Natural Science Foundation of China(31873036,32072958,31922084,31872960,32102789)the National Key Research and Development Project(2018YFD0900204,2018YFD0901201,2018YFD0900203,2018YFD0900201)the Consulting Research Projects of Hubei Institute of Chinese Engineering Development Strategies and Academic Divisions of the Chinese Academy of Sciences(2021-SM02-B-010)the China Agriculture Research System of MOF and MARA(CARS-45-07 and CARS-46)the Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology(2019FBZ04)the Youth Innovation Promotion Association CAS(2020334)。
文摘Aquaculture is one of the most efficient modes of animal protein production and plays an important role in global food security.Aquaculture animals exhibit extraordinarily diverse sexual phenotypes and underlying mechanisms,providing an ideal system to perform sex determination research,one of the important areas in life science.Moreover,sex is also one of the most valuable traits because sexual dimorphism in growth,size,and other economic characteristics commonly exist in aquaculture animals.Here,we synthesize current knowledge of sex determination mechanisms,sex chromosome evolution,reproduction strategies,and sexual dimorphism,and also review several approaches for sex control in aquaculture animals,including artificial gynogenesis,application of sex-specific or sex chromosome-linked markers,artificial sex reversal,as well as gene editing.We anticipate that better understanding of sex determination mechanisms and innovation of sex control approaches will facilitate sustainable development of aquaculture.
基金supported by grants from National Natural Science Foundation of China(31130057)‘Taishan Scholar’ Construction Project Special in Shandong ProvinceAgricultural Improved Variety Project of Shandong Province
文摘The Cynoglossus semilaevis(half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China.It has a female heterogametic sex determination system(ZW/ZZ) and environmental factors can induce sex-reversal of females to phenotypic males,suggesting that it is a promising model for the study of sex determination mechanisms.Additionally,females grow much faster than males and it is feasible to improve the aquaculture production through sex control techniques.This paper reviews the progress in research on sex determination mechanisms research in our laboratory.We have completed whole-genome sequencing and revealed the genome organization and sex chromosome evolution of C.semilaevis.A putative male determining gene dmrt1 was identified and DNA methylation was verified as having a crucial role in the sex reversal process.Genetic maps and sex-specific biomarkers have been used in a marker-assisted selection breeding program and for differentiation of the fish sex.Development and improvement of sex control technologies,including artificial gynogenesis and production of breeding fry with high proportion of females,is also reviewed.These research advances have provided insight into the regulation of sex determination and enabled efficient sex management in artificial culturing of C.semilaevis.
基金Supported by Project in Yunnan Branch of China National Tobacco Company(2010YN77)~~
文摘[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at the density of 26 m×26 m（one trap/676 m2） to carry out field control test.[Result] The amount of egg massed reduced by 62.16% after trapping by sex pheromone under the density,and the larvae population was equivalent to those in the conventional control field with pesticides,the control effect was continuous,stable and significant.The larger the control area was,the better the effect was.[Conclusion] Using sex pheromone could effectively control S.litura and reduce pesticide usage for over 80% with economic,safe and efficient features,which should be incorporated into the technical control system of S.litura.
基金Natural Science Foundation of Guangdong Province, China.
文摘Because the male silkworm is economically superior to the female in sericulture,male-silkworm rearing through sex control is an interesting question. In order to rearmale silkworms only, a balanced lethal system was established in Japan and former U.S.S.R.by utilizing the recessive female sex-linked gene. Tajima et al. eliminated femaleindividuals by means of mechanical or manual methods according to the sex-limitedegg color or sex-limited larva marking. In addition, Xu An-ying et al. tried to ob-tain only male individuals by inducing androgenesis. However, the above methodscannot yet be utilized in sericulture production because of their harmful influenceson the vitality of silkworm larvas or the hatching rate.
基金Supported by National Key Technology R&D Program(2011BAD16B10)Supporting Project of National Key Technology R&D Program(2015GJLS001NY)
文摘We studied the trapping effects on Ostrinia furnacalis from the aspects of different hanging heights and different color traps. The results suggested that the hanging height of 1.5 - 2.0 m received a better trapping effect, but trap color had little impact on trapping effect. The sex pheromone traps at different densities were placed in corn field. The results showed that sex pheromone lure could trap adult O. furnacalis, and it was feasible to apply sex pheromone in short term fore- cast. Meanwhile, it reduced the number of larvae and eggs, thereby reducing the damage of O. furnacalis. The lure number of 10 per hm2 was more appropriate, which could result in increasing yield of 8.61% compared with control
基金Supported by Sugar Crop Research System(CARS-170303)Training Project of Yunling Industry and Technology Leading Talents“Control of Sugarcane Harmful Organisms”(2018LJRC56)Special Fund of Agricultural Industry Research System in Yunnan Province
文摘This study was conducted to explore and seek high-efficiency and safe green control techniques on sugarcane borers,promote green control of diseases and pests of sugarcane and build a resource-conserving,environmentally friendly and sustainable governance system of diseases and pests of sugarcane.A new sex pheromone trap of sugarcane borers,abamectin· Bacillus thuringiensis (Bt) and tebufenozide were selected and applied in field trials.The use of the new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 0.05% abamectin·10 billion active gemmae/g B.thuringiensis (Bt) WP (1.8 kg/hm 2) or new sex pheromone trap of sugarcane borers (6 traps/hm 2) in combination with 200 g/L tebufenozide SC (1.5 L/hm 2) were the best in the sugarcane production.New sex pheromone trap of sugarcane borers should be installed in early march,and biological agents were mixed with water 900 kg per hectare and sprayed evenly sugarcane plants at the beginning of April.Their control effects of dead heart rate and bored stalk rate could be more than 69.98% and 49.09%,respectively,which were superior to that of the control pesticide 3.6% bisultap GR (90 kg/hm 2).The results showed that new sex pheromone trap of sugarcane borers in combination with abamectin· B.thuringiensis (Bt) or tebufenozide was the optimum mode of green control techniques on sugarcane borers.When it was alternately or coordinately used with other technology,it could delay the emergence and development of drug resistance,and it was worthy of widespread popularization and application in sugarcane area.
基金This research was supported by the National Natural Science Foundation of China(31672425)Shaanxi Province Key R&D Program(2018ZDXM-NY-043,2020ZDLNY02–04).
文摘Background:Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion.To derive greater economic benefit,farmers require more female offspring(does);however,the buck-to-doe offspring sex ratio is approximately 50%.At present,artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring.However,flow cytometry has not been successfully utilised for the separation of X/Y sperm aimed at sexing control in dairy goats.Results:In this study,a novel,simple goat sperm sexing technology that activates the toll-like receptor 7/8(TLR7/8),thereby inhibiting X-sperm motility,was investigated.Our results showed that the TLR7/8 coding goat Xchromosome was expressed in approximately 50%of round spermatids in the testis and sperm,as measured from cross-sections of the epididymis and ejaculate,respectively.Importantly,TLR7/8 was located at the tail of the Xsperm.Upon TLR7/8 activation,phosphorylated forms of glycogen synthase kinaseα/β(GSK3α/β)and nuclear factor kappa-B(NF-κB)were detected in the X-sperm,causing reduced mitochondrial activity,ATP levels,and sperm motility.High-motility Y-sperm segregated to the upper layer and the low-motility X-sperm,to the lower layer.Following in vitro fertilisation using the TLR7/8-activated sperm from the lower layer,80.52±6.75%of the embryos were XX females.The TLR7/8-activated sperm were subsequently used for in vivo embryo production via the superovulatory response;nine embryos were collected from the uterus of two does that conceived.Eight of these were XX embryos,and one was an XY embryo.Conclusions:Our study reveals a novel TLR7/8 signalling mechanism that affects X-sperm motility via the GSK3α/β-hexokinase pathway;this technique could be used to facilitate the efficient production of sexed dairy goat embryos.
文摘Out of 100 cases of intractable schizophrenia,57 cases were in the group ofComputer-Controlled Electric Acupuncture (CCEA) and 43 in the control group.The therapeutic re-sults showed that clinically CCEA had a remarkably effective rate of 40. 35% on refractoryschizophrenia,which had much better curative effects than those in the control group.Through dy-namic evaluation of brief psychiatric rating scale (BPRS),negative symptom scale (SANS) and posi-tive symptom scale (SAPS) for both groups before,during and after treatment,the results indicatedthat in CCEA group the scores of BPRS,SANS and SAPS after treatment were much less than thosebefore treatment,which were remarkably different from those in the control group (P【0.01).Lab-oratory examination of thyroxin and androgen of patients in both groups before and after treatmentfound a significant result.
文摘2022年5月以来,全球110多个既往非流行区国家和地区暴发猴痘疫情,通过男男性行为人群(Men who have sex with men,MSM)性接触及其聚集性活动传播扩散。2023年6月2日我国发现首例本土猴痘病例,截至11月30日累计发现1610例,通过开展监测、病例及其密切接触者追踪调查管理、重点人群健康教育干预等监测防控措施,10月份以来境内猴痘疫情已降至较低水平,但仍持续面临境外输入及境内低水平传播风险。为达到终止猴痘暴发及阻断人传人疫情的防控目标,需进一步加强病例发现、传播链调查、潜在暴露者识别、重点人群干预等工作,持续引导重点人群行为改变、多渠道提升监测发现能力。
