Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. T...Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. The activation of the Y-linked gene Sry （sex- determining region Y） and its downstream target Sox9 （Sry box-containing gene 9） triggers testis differentiation by stimulating the differentiation of Sertoli cells, which then direct testis morphogenesis. Once engaged, a genetic pathway promotes the testis development while actively suppressing genes involved in ovarian development. This review focuses on the events of testis determination and the struggle to maintain male fate in the face of antagonistic pressure from the underlying female programme.展开更多
This study was conducted retrospectively on a cohort of 68 patients with steroid 5α-reductase 2(SRD5A2)deficiency and 46,XY disorders of sex development(DSD).Whole-exon sequencing revealed 28 variants of SRD5A2,and f...This study was conducted retrospectively on a cohort of 68 patients with steroid 5α-reductase 2(SRD5A2)deficiency and 46,XY disorders of sex development(DSD).Whole-exon sequencing revealed 28 variants of SRD5A2,and further analysis identified seven novel mutants.The preponderance of variants was observed in exon 1 and exon 4,specifically within the nicotinamide adenine dinucleotide phosphate(NADPH)-binding region.Among the entire cohort,53 patients underwent initial surgery at Sichuan Provincial People’s Hospital(Chengdu,China).The external genitalia scores(EGS)of these participants varied from 2.0 to 11.0,with a mean of 6.8(standard deviation[s.d.]:2.5).Thirty patients consented to hormone testing.Their average testosterone-todihydrotestosterone(T/DHT)ratio was 49.3(s.d.:23.4).Genetic testing identified four patients with EGS scores between 6 and 9 as having this syndrome;and their T/DHT ratios were below the diagnostic threshold.Furthermore,assessments conducted using the crystal structure of human SRD5A2 have provided insights into the potential pathogenic mechanisms of these novel variants.These mechanisms include interference with NADPH binding(c.356G>C,c.365A>G,c.492C>G,and c.662T>G)and destabilization of the protein structure(c.727C>T).The c.446-1G>T and c.380delG variants were verified to result in large alterations in the transcripts.Seven novel variations were identified,and the variant database for the SRD5A2 gene was expanded.These findings contribute to the progress of diagnostic and therapeutic approaches for individuals with SRD5A2 deficiency.展开更多
Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usu...Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usually have distinct reproductive and dispersal modes responding to relevant selective forces.Here,we showed significant phenotypic divergence in a set of fitness-related traits among males but not among females in the introduced oriental fire-bellied toads(Bombina orientalis),which established a non-native population in Beijing after being introduced in 1927.Specifically,compared with the native counterparts,introduced males displayed younger age and reduced body size,but increased hindlimb length and eye size after controlling the age effect.As the toads have male-biased sex ratio and male-biased dispersal during breeding season,the observed shifts may suggest that the male-biased selective forces have induced increased energy allocation to dispersal and foraging,and thus decreased allocation to maintain longevity in males.Overall,our study provides evidence for the modification of fitness-related phenotypic traits in a non-native anuran population compared with native populations and extends our understanding of key trait divergence in established non-native amphibian under sexual-unbalanced selective forces.展开更多
This study aimed to explore the effectiveness of peer education in adolescent sexual health education.A scoping search was conducted in the databases of PubMed,Web of Science,Embase,Cochrane Library,SinoMed,CNKI,Wanfa...This study aimed to explore the effectiveness of peer education in adolescent sexual health education.A scoping search was conducted in the databases of PubMed,Web of Science,Embase,Cochrane Library,SinoMed,CNKI,Wanfang,and VIP databases to retrieve relevant literature published from the establishment of the database to January 2025 on sexual health education for adolescents in the form of peer education.A total of 11 literatures were finally included.The findings revealed that Peer education was mainly conducted in participatory forms such as role-playing,group discussion,and brainstorming,with a wide range of interventions.Peer education can enhance adolescents’level of sexual knowledge,sex-related self-efficacy and risk perception,improve sexual attitudes and sexual behavioral intentions,as well as reduce risky sexual behavior.In conclusion,Peer education has a positive impact on adolescents’sexual health and can be an effective way of adolescent sexual health education.展开更多
Objective:To assess public knowledge,attitudes,and behaviours regarding same-sex sexual intercourse(SSI)in relation to HIV/AIDS transmission prevention,especially in an inclusive cultural and religious country like In...Objective:To assess public knowledge,attitudes,and behaviours regarding same-sex sexual intercourse(SSI)in relation to HIV/AIDS transmission prevention,especially in an inclusive cultural and religious country like Indonesia,beyond established prevention methods like safe sex practices and Pre-Exposure Prophylaxis(PrEP).Methods:We collected cross-sectional online survey data from the Indonesian 18 years or older population in December 2021 and August 2022.We determined participants'knowledge,attitude,and behaviour scores toward SSI and HIV/AIDS prevention.We critically explored each score and used multiple linear regression to identify the predictive factors.Results:The survey included 386 adults[median age(IQR):22(20-35)years].The median(IQR)score was 90/100(80-100)for knowledge,43/50(39-46)for attitude and 70/70(70-70)for behaviour.The number of men who had sex with men(MSM)was 7/129(5.4%).Male sex,non-MSM,higher knowledge,and higher attitude scores were positively associated with higher behaviour scores(P<0.001).Conclusions:This study highlights the need for inclusive HIV/AIDS prevention strategies that respect cultural and religious values,reduce stigma,and improve healthcare access,while aligning with international guidelines and scientific evidence.展开更多
17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties si...17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties similar to testosterone,and the consequences of juvenile exposure on adult social behavior remain uncertain.Bisphenol A(BPA)acts as an estrogen-like hormone,compared to 17-TB.Three-week-old male Balb/c mice were exposed orally to 17-TB(100μg/(kg·day))and BPA(4 mg/(kg·day))for 28 days.Assessments of social interactions and a three-chamber test showed that 17-TB increased virility in male mice,intensified both male and female sexual behavior,and attracted and accepted female mice.It also increased social dominance through tube tests inmalemice and markedly activated the c-Fos^(+)immune response in themedial prefrontal cortex(mPFC)and basal amygdala(BLA).ELISA data showed that 17-TB and BPA exposure significantly affected serum gonadotropin-releasing hormone(GnRH),growth hormone(GH),estradiol(E2),and luteinizing hormone(LH)levels,as well as testicular lesions and androgen receptor(ARβ)and estrogen receptor(ERα)synthesis.Testicular transcriptomic analysis further confirmed that could disrupt steroid synthesis and linoleic acid-related biometabolic processes.These findings suggest the influence of 17-TB and BPA exposure on sexual behavior and fertility in male mice,possibly through modulation of the hypothalamic-pituitary-gonadal axis.This study provides insights relevant to human reproductive health and neuro-social behavioral research,and the potential risk of environmental disturbances should not be overlooked.展开更多
Fisher's theory predicts equal sex ratios at the end of parental care if the cost associated with raising offspring of each sex is equal.However,sex ratios have important evolutionary consequences and are often bi...Fisher's theory predicts equal sex ratios at the end of parental care if the cost associated with raising offspring of each sex is equal.However,sex ratios have important evolutionary consequences and are often biased for many factors.Reported sex ratios are often biased in raptors,which display various degrees of reversed sexual dimorphism,but there seems no consistent pattern in their offspring sex ratios.In this study,we investigated the offspring sex ratio of the Eurasian Kestrel(Falco tinnunculus) and tested whether the patterns of biased sex ratios were related to laying order,egg mass,hatching order,laying date or clutch size.The brood sex ratio of the Eurasian Kestrel(male) in eggs was 47.0%,not statistically biased from 0.5,but in fledglings it was 40.8%,significantly biased from 0.5(p = 0.029).At population level,both primary and secondary sex ratios did not depart from parity.We found that clutch size and egg mass affected the secondary brood sex ratio,i.e.,the larger the clutch size,the larger the number of males and eggs producing sons were heavier than eggs producing daughters.Laying date affected both the primary and secondary sex ratios,and laying earlier is associated with a greater proportion of males.展开更多
Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, ...Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to- female sex-reversal model of chick induced by diethylstilbestrol （DES） at onset of incubation （E0）. The results of semi- quantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene （Amh） expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.展开更多
Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interfe...Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.展开更多
Sex-determining systems show a striking diversity not only among species, but also among popula tions. In reptiles, sex-determina tion is a continuum, from tempera ture-dependent sex determination(TSD) to genetic sex ...Sex-determining systems show a striking diversity not only among species, but also among popula tions. In reptiles, sex-determina tion is a continuum, from tempera ture-dependent sex determination(TSD) to genetic sex determination(GSD).The multi-ocellated racerunner(Eremias multiocellata)is reported to be a cryptic ZZ/ZW chromosomal TSD species, with male-biased sex ratios at high temperatures in two Gansu populations. However, the generality of the sex-determining pattern in different populations of this species remains unclear. To investigate the mode of sex determina tion in a popula tion of E.multiocellata from the desert steppe of Inner Mongolia,we first identified sex chromosomes via comparative genomic hybridization(CGH). We then conducted a thermal manipulation experiment to determine the effect of gestation temperature on offspring sex ratios.From the CGH studies we found that lizards from the Inner Mongolia population possessed ZZ/ZW sex chromosomes. However, our thermal manipulation experiment showed that gestation temperature did not affect the sex ratio of neonates in this population. In combination, these results rule out TSD in the Inner Mongolia population of E. multiocellata, and suggest that there is widespread geographic variation in the sexdetermining system of this species.展开更多
A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by...A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by Surgery (SRS). Visual Analogue Scale from 0 to 10 has been used to assess sexual desire and satisfaction, number of sexual partners and number of sexual intercourses/ month have been evaluated to assess sexual activity. Moreover, after the surgery, the Sexual Desire Inventory-2 (SDI-2) was administered to patients and related to testosterone (total or free) levels. In FtM, after hysterectomy and bilateral ovariectomy, mean value of sexual desire (VAS 0 - 10) was 7.5 before beginning testosterone therapy, this value increased after six months of therapy and after one year. Just before SRS the value decreased, but increased to 8.42 after SRS. In the same patients the values (VAS 0 - 10) on sexual satisfaction were respectively 6.5, 6.9, 7.1, 6.5 and 7. Mean number of partners was 4, with 7.07 mean frequency of sexual intercourse/month, before starting therapy (after hysterectomy). During hormonal therapy (testosterone), No. of partners was 2.46 and 8.96 intercourses/month, while after SRS the partners were 1.25 and intercouses/month 7.91. MtF patients reported a sexual desire (VAS 0 - 10) before therapy of 7, this remained almost constant after 6 months, decreases after 12 months and just before surgery, and finally increased to 7.76 after SRS. For sexual satisfaction mean values of VAS were respectively: 6.1, 6.2, 6.3, 6, and 6.8. Regarding sexual activity, MtF report 3.81 mean No. of partners and 9.27 intercouses/month before the beginning of hormonal therapy, 1.36 partners and 4.36 intercourses after therapy, and 1.3 partners and 4.54 intercourses after SRS.展开更多
Empathy is crucial for communication and survival for individuals.Whether empathy in pain contagion shows sex differences and its underlying mechanisms remain unclear.Here,we report that pain contagion can occur in st...Empathy is crucial for communication and survival for individuals.Whether empathy in pain contagion shows sex differences and its underlying mechanisms remain unclear.Here,we report that pain contagion can occur in stranger female rats,but not in stranger males.Blocking oxytocin receptors in the anterior cingulate cortex(ACC)suppressed pain contagion in female strangers,while oxytocin administration induced pain contagion in male strangers.In vitro,corticosterone reduces neuronal activation by oxytocin.During male stranger interactions,higher corticosterone decreased oxytocin receptor-positive neuronal activity in the ACC,suppressing pain contagion.These findings highlight the role of oxytocin in pain contagion and suggest that sex differences in empathy may be determined by the balance of oxytocin and corticosterone in the ACC.This study suggests an approach for the treatment of certain mental disorders associated with abnormal empathy,such as autism and depression.展开更多
The study of temperament and behavioral syndromes in insects is still in its early stage,and research conducted to date has mainly focused on locomotor activity and thanatosis.Dung beetles have been the subject of ext...The study of temperament and behavioral syndromes in insects is still in its early stage,and research conducted to date has mainly focused on locomotor activity and thanatosis.Dung beetles have been the subject of extensive behavioral studies;however,very few studies have addressed the expression of temperament.Those doing so only looked at subsocial and sexual horn dimorphic species,suggesting subsociality and/or sexual horn-dimorphism as possible facilitators of temperament expression.To test this assumption,we conducted a temperament study in a hornless,non-subsocial species,namely Geotrupes mutator(Marsham,1802).We set up laboratory tests to evaluate 3 behaviors(activity,thanatosis,and distress calls)through the measurement of 7 distinct behavioral traits(3 activity-,1 thanatosis-,and 3 call-related traits).We found high levels of individual repeatability in all activity-and thanatosis-related traits.We also identified behavioral differences between individuals,which may reflect differences in temperament.Statistical analyses revealed a negative correlation between activity and thanatosis.These results show that the temperament and behavioral syndromes related to activity and thanatosis may also be expressed in dung beetle species that are neither subsocial nor sexual horn dimorphic.By contrast,we only found one of 3 sound-related traits tested(frequency)to be clearly repeatable.Males and females presented a different structure of the stridulatory apparatus,suggesting that morphology may affect the frequency of sounds emitted.These results indicate that certain sound traits might not be good descriptors of individual temperament revealing the need for future research addressing the role of bioacoustics.展开更多
BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex educat...BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex education started relatively late,yet there are many problems in the development of sex education in China;for example,college students lack knowledge of sexuality.AIM To explore the perception of sex education among medical college students.METHODS Students majoring in medicine in a medical school were selected as the survey subjects.Anonymous online questionnaires were used to conduct the survey,and the results were analyzed using GraphPad Prism,SPSS,Microsoft Excel,and other software.The questionnaire was administered to understand the source of sexual knowledge,sexual responsibility,mastery of sexual knowledge,and distress caused by sexual problems.RESULTS Most students majoring in medicine had no formal sex education,lacked sexual knowledge,or had a biased understanding of sexual responsibility.This study analyzed future research trends in sex education based on relevant achievements in the Chinese context and abroad to further realize the practical significance and value of sex education popularization in China and provide recommendations for parents and schools at different levels.CONCLUSION Sex education should be conducted among college students,and medical colleges and universities should strengthen scientific sex education.展开更多
BACKGROUND There is increasing incidence of alcohol-associated liver disease in females.Despite this recent increased incidence,there is a paucity of research on the clinical course and outcomes of alcohol-associated ...BACKGROUND There is increasing incidence of alcohol-associated liver disease in females.Despite this recent increased incidence,there is a paucity of research on the clinical course and outcomes of alcohol-associated hepatitis(AH)in females compared to males.AIM To assess if there may be sex differences in severity,outcomes,and healthcare utilization for patients hospitalized for AH.METHODS This study used ICD-9-CM and ICD-10-CM codes to retrospectively identify inpatient encounters for AH at the University of Kentucky from 2012-2021 and obtained data on patient demographics and clinical outcomes.Encounters were cohorted by patient sex and differences in patient demographics and clinical outcomes were assessed.Multivariate logistic regression models were constructed to assess risk of mortality,sepsis,and mechanical ventilation during the encounter.RESULTS Of 1386 subjects,511(36.9%)were female and 875(63.1%)were male.Both sexes had similar baseline characteristics of race/ethnicity,discriminant function score,model of end-stage liver disease score,and length of hospital stay.However,the incidence of urinary tract infection,sepsis,and norepinephrine administration was significantly higher for females.Males had a significantly higher incidence of esophageal variceal bleed.On multivariate logistic regression analysis,females had higher odds of encounter sepsis(OR 1.41;95%CI:1.064-1.869)and mechanical ventilation(OR 1.352;95%CI:1.006-1.816).Odds of encounter mortality were significantly increased in encounters with sepsis(OR 2.309;95%CI:1.419-3.757)and mechanical ventilation(OR 9.301;95%CI:5.724-15.114).CONCLUSION This study shows sex-based differences in AH outcomes at the University of Kentucky.Future studies are warranted to assess whether tailoring treatments will improve clinical outcomes in females with AH.展开更多
BACKGROUND The integrity and functionality of the hepatic microcirculation are essential for maintaining liver health,which is influenced by sex and genetic background.Understanding these variations is crucial for add...BACKGROUND The integrity and functionality of the hepatic microcirculation are essential for maintaining liver health,which is influenced by sex and genetic background.Understanding these variations is crucial for addressing disparities in liver disease outcomes.AIM To investigate the sexual dimorphism and genetic heterogeneity of liver microcirculatory function in mice.METHODS We assessed hepatic microhemodynamics in BALB/c,C57BL/6J,and KM mouse strains using laser Doppler flowmetry and wavelet analysis.We analyzed the serum levels of alanine transaminase,glutamic acid aminotransferase,total bile acid,total protein,alkaline phosphatase,and glucose.Histological and immunohistochemical staining were employed to quantify microvascular density and the expression levels of cluster of differentiation(CD)31,and estrogen receptorα,andβ.Statistical analyses,including the Mantel test and Pearson correlation,were conducted to determine the relationships among hepatic function,microcirculation,and marcocirculation between different sexes and across genetic backgrounds.RESULTS We identified sex-based disparities in hepatic microhemodynamics across all strains,with males exhibiting higher microvascular perfusion and erythrocyte concentration,but lower blood velocity.Strain-specific differences were evident,particularly in the endothelial oscillatory characteristics of the erythrocyte concentration.No sexdependent differences in estrogen receptor expression were observed,while significant variations in CD31 expression and microvascular density were observed.The correlations highlighted relationships between hepatic microhemodynamics and liver function indicators.CONCLUSION Our findings indicate the influence of genetic and sex differences on hepatic microcirculation and liver function,highlighting the necessity of incorporating both genetic background and sex into hepatic physiology studies and potential liver disease management strategies.展开更多
Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural count...Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.展开更多
文摘Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. The activation of the Y-linked gene Sry （sex- determining region Y） and its downstream target Sox9 （Sry box-containing gene 9） triggers testis differentiation by stimulating the differentiation of Sertoli cells, which then direct testis morphogenesis. Once engaged, a genetic pathway promotes the testis development while actively suppressing genes involved in ovarian development. This review focuses on the events of testis determination and the struggle to maintain male fate in the face of antagonistic pressure from the underlying female programme.
基金supported by the Sichuan Science and Technology Program(No.2022JDZH0029 to JYY)the Special Fund for Clinical Research and Translational Medicine from Chinese Academy of Medical Sciences(No.2022-I2M-C&T-B-117 to JYY)the Sichuan Key Research and Development Project from the Department of Science and Technology of Sichuan Province(No.2022YFS0237 to YMT).
文摘This study was conducted retrospectively on a cohort of 68 patients with steroid 5α-reductase 2(SRD5A2)deficiency and 46,XY disorders of sex development(DSD).Whole-exon sequencing revealed 28 variants of SRD5A2,and further analysis identified seven novel mutants.The preponderance of variants was observed in exon 1 and exon 4,specifically within the nicotinamide adenine dinucleotide phosphate(NADPH)-binding region.Among the entire cohort,53 patients underwent initial surgery at Sichuan Provincial People’s Hospital(Chengdu,China).The external genitalia scores(EGS)of these participants varied from 2.0 to 11.0,with a mean of 6.8(standard deviation[s.d.]:2.5).Thirty patients consented to hormone testing.Their average testosterone-todihydrotestosterone(T/DHT)ratio was 49.3(s.d.:23.4).Genetic testing identified four patients with EGS scores between 6 and 9 as having this syndrome;and their T/DHT ratios were below the diagnostic threshold.Furthermore,assessments conducted using the crystal structure of human SRD5A2 have provided insights into the potential pathogenic mechanisms of these novel variants.These mechanisms include interference with NADPH binding(c.356G>C,c.365A>G,c.492C>G,and c.662T>G)and destabilization of the protein structure(c.727C>T).The c.446-1G>T and c.380delG variants were verified to result in large alterations in the transcripts.Seven novel variations were identified,and the variant database for the SRD5A2 gene was expanded.These findings contribute to the progress of diagnostic and therapeutic approaches for individuals with SRD5A2 deficiency.
基金supported by the National Natural Science Foundation of China (32171657,32301459)the grant from Institute of Zoology,Chinese Academy of Sciences (2023IOZ0104)the grant from Youth Innovation Promotion Association of Chinese Academy of Sciences (Y201920)。
文摘Successful non-native species can exhibit rapid divergence of key phenotypic traits to facilitate population persistence and further range expansion.Such phenotypic shifts can be sex-dependent as males and females usually have distinct reproductive and dispersal modes responding to relevant selective forces.Here,we showed significant phenotypic divergence in a set of fitness-related traits among males but not among females in the introduced oriental fire-bellied toads(Bombina orientalis),which established a non-native population in Beijing after being introduced in 1927.Specifically,compared with the native counterparts,introduced males displayed younger age and reduced body size,but increased hindlimb length and eye size after controlling the age effect.As the toads have male-biased sex ratio and male-biased dispersal during breeding season,the observed shifts may suggest that the male-biased selective forces have induced increased energy allocation to dispersal and foraging,and thus decreased allocation to maintain longevity in males.Overall,our study provides evidence for the modification of fitness-related phenotypic traits in a non-native anuran population compared with native populations and extends our understanding of key trait divergence in established non-native amphibian under sexual-unbalanced selective forces.
基金supported by the National Natural Science Foundation China under Grant number 72204129Natural Science Foundation of Shandong Province under Grant number ZR2021QG029.
文摘This study aimed to explore the effectiveness of peer education in adolescent sexual health education.A scoping search was conducted in the databases of PubMed,Web of Science,Embase,Cochrane Library,SinoMed,CNKI,Wanfang,and VIP databases to retrieve relevant literature published from the establishment of the database to January 2025 on sexual health education for adolescents in the form of peer education.A total of 11 literatures were finally included.The findings revealed that Peer education was mainly conducted in participatory forms such as role-playing,group discussion,and brainstorming,with a wide range of interventions.Peer education can enhance adolescents’level of sexual knowledge,sex-related self-efficacy and risk perception,improve sexual attitudes and sexual behavioral intentions,as well as reduce risky sexual behavior.In conclusion,Peer education has a positive impact on adolescents’sexual health and can be an effective way of adolescent sexual health education.
基金funded by the Faculty of Medicine,Universitas Andalas(grant number 668/UN.16.02.D/PP/2022).
文摘Objective:To assess public knowledge,attitudes,and behaviours regarding same-sex sexual intercourse(SSI)in relation to HIV/AIDS transmission prevention,especially in an inclusive cultural and religious country like Indonesia,beyond established prevention methods like safe sex practices and Pre-Exposure Prophylaxis(PrEP).Methods:We collected cross-sectional online survey data from the Indonesian 18 years or older population in December 2021 and August 2022.We determined participants'knowledge,attitude,and behaviour scores toward SSI and HIV/AIDS prevention.We critically explored each score and used multiple linear regression to identify the predictive factors.Results:The survey included 386 adults[median age(IQR):22(20-35)years].The median(IQR)score was 90/100(80-100)for knowledge,43/50(39-46)for attitude and 70/70(70-70)for behaviour.The number of men who had sex with men(MSM)was 7/129(5.4%).Male sex,non-MSM,higher knowledge,and higher attitude scores were positively associated with higher behaviour scores(P<0.001).Conclusions:This study highlights the need for inclusive HIV/AIDS prevention strategies that respect cultural and religious values,reduce stigma,and improve healthcare access,while aligning with international guidelines and scientific evidence.
基金supported by the National Natural Science Foundation of China(Nos.62027812 and 62273186).
文摘17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties similar to testosterone,and the consequences of juvenile exposure on adult social behavior remain uncertain.Bisphenol A(BPA)acts as an estrogen-like hormone,compared to 17-TB.Three-week-old male Balb/c mice were exposed orally to 17-TB(100μg/(kg·day))and BPA(4 mg/(kg·day))for 28 days.Assessments of social interactions and a three-chamber test showed that 17-TB increased virility in male mice,intensified both male and female sexual behavior,and attracted and accepted female mice.It also increased social dominance through tube tests inmalemice and markedly activated the c-Fos^(+)immune response in themedial prefrontal cortex(mPFC)and basal amygdala(BLA).ELISA data showed that 17-TB and BPA exposure significantly affected serum gonadotropin-releasing hormone(GnRH),growth hormone(GH),estradiol(E2),and luteinizing hormone(LH)levels,as well as testicular lesions and androgen receptor(ARβ)and estrogen receptor(ERα)synthesis.Testicular transcriptomic analysis further confirmed that could disrupt steroid synthesis and linoleic acid-related biometabolic processes.These findings suggest the influence of 17-TB and BPA exposure on sexual behavior and fertility in male mice,possibly through modulation of the hypothalamic-pituitary-gonadal axis.This study provides insights relevant to human reproductive health and neuro-social behavioral research,and the potential risk of environmental disturbances should not be overlooked.
基金supported by the National Natural Science Foundation of China (Grant No. 30400047)the Training Fund of Northeast Normal University Scientific Innovation Project (Grant No. 07013)the Jilin Provincial Science and Technology Department (Grant No. 20070558 and 20090575)
文摘Fisher's theory predicts equal sex ratios at the end of parental care if the cost associated with raising offspring of each sex is equal.However,sex ratios have important evolutionary consequences and are often biased for many factors.Reported sex ratios are often biased in raptors,which display various degrees of reversed sexual dimorphism,but there seems no consistent pattern in their offspring sex ratios.In this study,we investigated the offspring sex ratio of the Eurasian Kestrel(Falco tinnunculus) and tested whether the patterns of biased sex ratios were related to laying order,egg mass,hatching order,laying date or clutch size.The brood sex ratio of the Eurasian Kestrel(male) in eggs was 47.0%,not statistically biased from 0.5,but in fledglings it was 40.8%,significantly biased from 0.5(p = 0.029).At population level,both primary and secondary sex ratios did not depart from parity.We found that clutch size and egg mass affected the secondary brood sex ratio,i.e.,the larger the clutch size,the larger the number of males and eggs producing sons were heavier than eggs producing daughters.Laying date affected both the primary and secondary sex ratios,and laying earlier is associated with a greater proportion of males.
基金supported by the National Key Technology R&D Program of China (2007BAD72B01)the National High Technology R&D Program of China (2011AA100306)
文摘Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to- female sex-reversal model of chick induced by diethylstilbestrol （DES） at onset of incubation （E0）. The results of semi- quantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene （Amh） expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.
文摘Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical activity.Compared with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower cost.However,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM technology.Pain,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex differences.Despite clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are lacking.The aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG signaling.Methods This study utilized adult male and female Sprague-Dawley rats.Inflammatory pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving saline.Pain behavior was assessed using von Frey filaments at baseline and 1 h post-injection.For MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation phase.Data analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency range.Results MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA injections.Mechanical stimulation elevated alpha activity in both male and female rats pre-and post-saline/CFA injections.Saline/CFA injections augmented average power in both sexes compared to pre-injection states.Remarkably,female rats exhibited higher average spectral power 1 h after CFA injection than after saline injection during resting states.Furthermore,despite comparable pain thresholds measured by classical pain behavioral tests post-CFA treatment,female rats displayed higher average power than males in the resting state after CFA injection.Conclusion These results imply an enhanced perception of inflammatory pain in female rats compared to their male counterparts.Our study exhibits sex differences in alpha activities following CFA injection,highlighting heightened brain alpha activity in female rats during acute inflammatory pain in the resting state.Our study provides a method for OPM-based MEG recordings to be used to study brain activity in anaesthetized animals.In addition,the findings of this study contribute to a deeper understanding of pain-related neural activity and pain sex differences.
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB31000000)the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2019QZKK0501)Joint Grant from Chinese Academy of Sciences-People’s Government of Qinghai Province on Sanjiangyuan National Park(LHZX-2020-01)。
文摘Sex-determining systems show a striking diversity not only among species, but also among popula tions. In reptiles, sex-determina tion is a continuum, from tempera ture-dependent sex determination(TSD) to genetic sex determination(GSD).The multi-ocellated racerunner(Eremias multiocellata)is reported to be a cryptic ZZ/ZW chromosomal TSD species, with male-biased sex ratios at high temperatures in two Gansu populations. However, the generality of the sex-determining pattern in different populations of this species remains unclear. To investigate the mode of sex determina tion in a popula tion of E.multiocellata from the desert steppe of Inner Mongolia,we first identified sex chromosomes via comparative genomic hybridization(CGH). We then conducted a thermal manipulation experiment to determine the effect of gestation temperature on offspring sex ratios.From the CGH studies we found that lizards from the Inner Mongolia population possessed ZZ/ZW sex chromosomes. However, our thermal manipulation experiment showed that gestation temperature did not affect the sex ratio of neonates in this population. In combination, these results rule out TSD in the Inner Mongolia population of E. multiocellata, and suggest that there is widespread geographic variation in the sexdetermining system of this species.
文摘A group of 39 Transgender subjects, 17 Female to Male (FtM) and 22 Male to Female (MtF), have been evaluated about their sexual desire, activity, and satisfaction during the period before and after Sex Reassignment by Surgery (SRS). Visual Analogue Scale from 0 to 10 has been used to assess sexual desire and satisfaction, number of sexual partners and number of sexual intercourses/ month have been evaluated to assess sexual activity. Moreover, after the surgery, the Sexual Desire Inventory-2 (SDI-2) was administered to patients and related to testosterone (total or free) levels. In FtM, after hysterectomy and bilateral ovariectomy, mean value of sexual desire (VAS 0 - 10) was 7.5 before beginning testosterone therapy, this value increased after six months of therapy and after one year. Just before SRS the value decreased, but increased to 8.42 after SRS. In the same patients the values (VAS 0 - 10) on sexual satisfaction were respectively 6.5, 6.9, 7.1, 6.5 and 7. Mean number of partners was 4, with 7.07 mean frequency of sexual intercourse/month, before starting therapy (after hysterectomy). During hormonal therapy (testosterone), No. of partners was 2.46 and 8.96 intercourses/month, while after SRS the partners were 1.25 and intercouses/month 7.91. MtF patients reported a sexual desire (VAS 0 - 10) before therapy of 7, this remained almost constant after 6 months, decreases after 12 months and just before surgery, and finally increased to 7.76 after SRS. For sexual satisfaction mean values of VAS were respectively: 6.1, 6.2, 6.3, 6, and 6.8. Regarding sexual activity, MtF report 3.81 mean No. of partners and 9.27 intercouses/month before the beginning of hormonal therapy, 1.36 partners and 4.36 intercourses after therapy, and 1.3 partners and 4.54 intercourses after SRS.
基金supported by the Shenzhen.Science and Technology Program(JCYJ20220818100615033,JCYJ20200109141433384,and KQTD20200820113040070)the National Natural Science Foundation of China(NSFC82471250 and 82350710225).
文摘Empathy is crucial for communication and survival for individuals.Whether empathy in pain contagion shows sex differences and its underlying mechanisms remain unclear.Here,we report that pain contagion can occur in stranger female rats,but not in stranger males.Blocking oxytocin receptors in the anterior cingulate cortex(ACC)suppressed pain contagion in female strangers,while oxytocin administration induced pain contagion in male strangers.In vitro,corticosterone reduces neuronal activation by oxytocin.During male stranger interactions,higher corticosterone decreased oxytocin receptor-positive neuronal activity in the ACC,suppressing pain contagion.These findings highlight the role of oxytocin in pain contagion and suggest that sex differences in empathy may be determined by the balance of oxytocin and corticosterone in the ACC.This study suggests an approach for the treatment of certain mental disorders associated with abnormal empathy,such as autism and depression.
基金supported by the project CN_00000033 funded under the National Recovery and Resilience Plan(NRRP)Mission 4 Component 2 Investment 1.4-Call for tender No.3138 of 16 December 2021,rectified by Decree n.3175 of 18 December 2021 of the Italian Ministry of University and Research funded by the European UnionNextGenerationEU.
文摘The study of temperament and behavioral syndromes in insects is still in its early stage,and research conducted to date has mainly focused on locomotor activity and thanatosis.Dung beetles have been the subject of extensive behavioral studies;however,very few studies have addressed the expression of temperament.Those doing so only looked at subsocial and sexual horn dimorphic species,suggesting subsociality and/or sexual horn-dimorphism as possible facilitators of temperament expression.To test this assumption,we conducted a temperament study in a hornless,non-subsocial species,namely Geotrupes mutator(Marsham,1802).We set up laboratory tests to evaluate 3 behaviors(activity,thanatosis,and distress calls)through the measurement of 7 distinct behavioral traits(3 activity-,1 thanatosis-,and 3 call-related traits).We found high levels of individual repeatability in all activity-and thanatosis-related traits.We also identified behavioral differences between individuals,which may reflect differences in temperament.Statistical analyses revealed a negative correlation between activity and thanatosis.These results show that the temperament and behavioral syndromes related to activity and thanatosis may also be expressed in dung beetle species that are neither subsocial nor sexual horn dimorphic.By contrast,we only found one of 3 sound-related traits tested(frequency)to be clearly repeatable.Males and females presented a different structure of the stridulatory apparatus,suggesting that morphology may affect the frequency of sounds emitted.These results indicate that certain sound traits might not be good descriptors of individual temperament revealing the need for future research addressing the role of bioacoustics.
基金Supported by Scientific Research Project of College Students in Liaoning Province,No.S202410164004.
文摘BACKGROUND Sex education was introduced early in foreign countries.For example,there is a more systematic sex education system abroad,which can better achieve the popularization of sex education.China's sex education started relatively late,yet there are many problems in the development of sex education in China;for example,college students lack knowledge of sexuality.AIM To explore the perception of sex education among medical college students.METHODS Students majoring in medicine in a medical school were selected as the survey subjects.Anonymous online questionnaires were used to conduct the survey,and the results were analyzed using GraphPad Prism,SPSS,Microsoft Excel,and other software.The questionnaire was administered to understand the source of sexual knowledge,sexual responsibility,mastery of sexual knowledge,and distress caused by sexual problems.RESULTS Most students majoring in medicine had no formal sex education,lacked sexual knowledge,or had a biased understanding of sexual responsibility.This study analyzed future research trends in sex education based on relevant achievements in the Chinese context and abroad to further realize the practical significance and value of sex education popularization in China and provide recommendations for parents and schools at different levels.CONCLUSION Sex education should be conducted among college students,and medical colleges and universities should strengthen scientific sex education.
文摘BACKGROUND There is increasing incidence of alcohol-associated liver disease in females.Despite this recent increased incidence,there is a paucity of research on the clinical course and outcomes of alcohol-associated hepatitis(AH)in females compared to males.AIM To assess if there may be sex differences in severity,outcomes,and healthcare utilization for patients hospitalized for AH.METHODS This study used ICD-9-CM and ICD-10-CM codes to retrospectively identify inpatient encounters for AH at the University of Kentucky from 2012-2021 and obtained data on patient demographics and clinical outcomes.Encounters were cohorted by patient sex and differences in patient demographics and clinical outcomes were assessed.Multivariate logistic regression models were constructed to assess risk of mortality,sepsis,and mechanical ventilation during the encounter.RESULTS Of 1386 subjects,511(36.9%)were female and 875(63.1%)were male.Both sexes had similar baseline characteristics of race/ethnicity,discriminant function score,model of end-stage liver disease score,and length of hospital stay.However,the incidence of urinary tract infection,sepsis,and norepinephrine administration was significantly higher for females.Males had a significantly higher incidence of esophageal variceal bleed.On multivariate logistic regression analysis,females had higher odds of encounter sepsis(OR 1.41;95%CI:1.064-1.869)and mechanical ventilation(OR 1.352;95%CI:1.006-1.816).Odds of encounter mortality were significantly increased in encounters with sepsis(OR 2.309;95%CI:1.419-3.757)and mechanical ventilation(OR 9.301;95%CI:5.724-15.114).CONCLUSION This study shows sex-based differences in AH outcomes at the University of Kentucky.Future studies are warranted to assess whether tailoring treatments will improve clinical outcomes in females with AH.
基金Supported by the Beijing Municipal Natural Science Foundation,No.7212068the National Natural Science Foundation of China,No.81900747.
文摘BACKGROUND The integrity and functionality of the hepatic microcirculation are essential for maintaining liver health,which is influenced by sex and genetic background.Understanding these variations is crucial for addressing disparities in liver disease outcomes.AIM To investigate the sexual dimorphism and genetic heterogeneity of liver microcirculatory function in mice.METHODS We assessed hepatic microhemodynamics in BALB/c,C57BL/6J,and KM mouse strains using laser Doppler flowmetry and wavelet analysis.We analyzed the serum levels of alanine transaminase,glutamic acid aminotransferase,total bile acid,total protein,alkaline phosphatase,and glucose.Histological and immunohistochemical staining were employed to quantify microvascular density and the expression levels of cluster of differentiation(CD)31,and estrogen receptorα,andβ.Statistical analyses,including the Mantel test and Pearson correlation,were conducted to determine the relationships among hepatic function,microcirculation,and marcocirculation between different sexes and across genetic backgrounds.RESULTS We identified sex-based disparities in hepatic microhemodynamics across all strains,with males exhibiting higher microvascular perfusion and erythrocyte concentration,but lower blood velocity.Strain-specific differences were evident,particularly in the endothelial oscillatory characteristics of the erythrocyte concentration.No sexdependent differences in estrogen receptor expression were observed,while significant variations in CD31 expression and microvascular density were observed.The correlations highlighted relationships between hepatic microhemodynamics and liver function indicators.CONCLUSION Our findings indicate the influence of genetic and sex differences on hepatic microcirculation and liver function,highlighting the necessity of incorporating both genetic background and sex into hepatic physiology studies and potential liver disease management strategies.
基金supported by the National Natural Science Foundation of China(NSFC,31971413)to D.L.
文摘Numerous bird species have adapted to rapid urbanization,navigating challenges introduced by novel stressors.While prior research has explored how urban exploiters adjust their physiology relative to their rural counterparts during breeding season,the modulation of multiple physiological markers outside of reproduction,and the maintenance of sex-specific differences,remain less understood.Using an urban exploiter,the Eurasian tree sparrow(ETS;Passer montanus),we investigated how both sexes of urban and rural populations modulate twenty-two physiological markers of nutrition,body condition,oxidative stress,and antioxidative responses during pre-breeding stage.Our results showed that urban ETSs displayed distinct glucolipid metabolism compared to rural ETSs,characterized by higher levels of free fatty acids,β-hydroxybutyric acid,high-density lipoprotein(HDL),and total cholesterol,alongside lower levels of triglycerides,glucose(Glu),and fat content(FC).No significant differences were observed in other physiological markers.Additionally,both sexes of urban ETSs exhibited similar profiles across these markers,unlike rural populations where females showed higher Glu and total protein levels and lower HDL and FC levels than males.Our findings suggest that birds adjust their nutritional physiological markers in response to urbanization,likely due to dietary differences between urban and rural environments.Furthermore,sex differences in physiological markers of nutrition,body condition,oxidative stress,and antioxidant responses appear to diminish in urban settings.
