Nowadays,biological databases are playing an increasingly critical role in biological research.Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-...Nowadays,biological databases are playing an increasingly critical role in biological research.Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-based chemical synthesis.The lack of a dedicated public database has made access to and reanalysis of M.thermophila data difficult.To bridge this gap,we developed MTD(https://mtd.biodesign.ac.cn/),a cloud-based omics database and interactive platform for M.thermophila.MTD integrates comprehensive genome annotations,sequence-based predictions,transcriptome data,curated experimental descriptions,and bioinformatics analysis tools,offering a comprehensive,one-stop solution with a‘top-down’search strategy to streamline M.thermophila research.The platform supports data reproduction,rapid querying,and in-depth mining of existing tran-scriptome datasets.Based on analyses using data and tools in MTD,we identified shifts in metabolic allocation in a glucoamylase hyperproduction strain of M.thermophila,highlighting changes in fatty acid biosynthesis and amino acids biosynthesis pathways,which provide new insights into the underlying phenotypic alterations.As a pioneering resource,MTD marks a key advancement in M.thermophila research and sets the model for developing similar databases for other species.展开更多
基金funded by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDC0110300)the National Key R&D Program of China(2023YFC3403602 and 2022YFC2106000)+2 种基金National Natural Science Foundation of China(32300529,32270100,and 32271481)the Innovation Fund of Haihe Laboratory of Synthetic Biology(22HHSWSS00014)the Tianjin Synthetic Biotechnology Inno-vation Capacity Improvement Project(TSBICIP-PTJJ-007-12).
文摘Nowadays,biological databases are playing an increasingly critical role in biological research.Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-based chemical synthesis.The lack of a dedicated public database has made access to and reanalysis of M.thermophila data difficult.To bridge this gap,we developed MTD(https://mtd.biodesign.ac.cn/),a cloud-based omics database and interactive platform for M.thermophila.MTD integrates comprehensive genome annotations,sequence-based predictions,transcriptome data,curated experimental descriptions,and bioinformatics analysis tools,offering a comprehensive,one-stop solution with a‘top-down’search strategy to streamline M.thermophila research.The platform supports data reproduction,rapid querying,and in-depth mining of existing tran-scriptome datasets.Based on analyses using data and tools in MTD,we identified shifts in metabolic allocation in a glucoamylase hyperproduction strain of M.thermophila,highlighting changes in fatty acid biosynthesis and amino acids biosynthesis pathways,which provide new insights into the underlying phenotypic alterations.As a pioneering resource,MTD marks a key advancement in M.thermophila research and sets the model for developing similar databases for other species.
