MnO2-impregnated carbon-coated Fe3 O4(Fe3O4/C/MnO2)nanocomposites with a good core-shell structure were prepared by direct oxidation of carbon-coated Fe3 O4(Fe3O4/C)microspheres with KMnO4 in alkaline solution and app...MnO2-impregnated carbon-coated Fe3 O4(Fe3O4/C/MnO2)nanocomposites with a good core-shell structure were prepared by direct oxidation of carbon-coated Fe3 O4(Fe3O4/C)microspheres with KMnO4 in alkaline solution and applied to adsorb bovine serum albumin(BSA).X-ray diffraction(XRD),transmission electron microscope(TEM),Fourier transform infrared spectrometer(FTIR),vibrating sample magnetometer(VSM)and thermogravimetric analyzer(TGA)tests show that Fe3O4/C microspheres were newly functionalized via the oxidation by KMnO4.Fe3O4/C/MnO2 nanocomposites exhibit a higher adsorption capacity for BSA than Fe3O4/C microspheres and the maximum adsorption of BSA on them occurs at pH 4.7,which is the isoelectric point of BSA.Langmuir isotherm model describes the adsorption of BSA better than Freundlich model and Temkin model,and the kinetics data fit well with the pseudo-second-order model.展开更多
AIM: To help clarifying the possibility of connective-tissue diseases in men with penile or testicular prostheses. METHODS: Eight patients underwent inflatable penile prostheses and 15, testicular prostheses consented...AIM: To help clarifying the possibility of connective-tissue diseases in men with penile or testicular prostheses. METHODS: Eight patients underwent inflatable penile prostheses and 15, testicular prostheses consented to the study. Their medical records were reviewed and a follow-up interview and physical and serological examinations were performed. RESULTS: In patients with penile prostheses, there was no abnormal antinuclear antibody (ANA) or IgM elevation. The serum levels of the rheumatoid factor (RF), C4, IgA and IgG were abnormal in one patient, and the levels of erythrocyte sedimentation rate (ESR) and C3, abnormal in two. Four had elevated IgE. In patients with testicular prostheses, there was no abnormal RF, ANA or IgM. The serum levels of ESR and IgA were abnormal in two, and three had abnormal C4, ten abnormal C3, and eleven decreased IgG. All had increased IgE. Men with penile prostheses had higher serum levels of IgG and IgM than those with testicular prostheses (P=0.001, P=0.016, respectively). The rates of abnormal values of IgE and IgG were higher in men with testicular prostheses than in men with penile prostheses (P=0.008, P=0.009, respectively). Physical examination was normal in all patients and nobody had documented symptoms pertinent to connective-tissue diseases. CONCLUSION: Our findings suggest that the risk of connective-tissue diseases is not higher in patients wearing prostheses as the ANA is negative and there is no apparent manifestation suggestive of connective-tissue diseases.展开更多
Using isoelective focusing in immobilized pH gradients and immunoblot, C3 phenotypes (F, FS, S) and C3 HAV4-1 monoclonal (F±S±) phenotypes were performed in 90 patients with IgA glomerulonephrits,(G.N.).incl...Using isoelective focusing in immobilized pH gradients and immunoblot, C3 phenotypes (F, FS, S) and C3 HAV4-1 monoclonal (F±S±) phenotypes were performed in 90 patients with IgA glomerulonephrits,(G.N.).including 49 IgA G. N.hypertensive (H.T.) patients and 41 IgA G. N. normotensive (N.T.) patients, and in 224 normal subjects (N.S.). A significant difference of C3 phenotype distribution between both IgA G. N.(hypertensive and normotensive) and N. S. was .found (P<0.01,P<0.01respectively).In monoclonal C3 HAV4-1(±) distribution significant difference between IgA H. T.and N.S.was observed (P<0.01). Furthermore, F and S allele .frequency of IgA G. N. including HT and NT is significantly. different (P<0.05). This data suggests that hypertensive patients with IgA G. N. seems to be related io the abnormal C3 genetic factors and if this gene distributions can be used as a predictor for the prognosis still needs futher investigations.展开更多
Background:Aberrant activation of the complement system plays an important role in the pathogenesis and development of immunoglobulin A nephropathy(IgAN).The relationship between serum complement and the clinical-hist...Background:Aberrant activation of the complement system plays an important role in the pathogenesis and development of immunoglobulin A nephropathy(IgAN).The relationship between serum complement and the clinical-histopathological features and outcomes of IgAN is controversial.This retrospective study aimed to examine the relationship between the complement 3/4(C3/C4)ratio and the clinicopathologic changes and prognosis of patients with IgAN.Methods:A total of 397 patients with primary IgAN from January 2007 to December 2012 at the Chinese People's Liberation Army General Hospital were included in this study.The correlation test and Chi-square test or one-way analysis of variance test were performed to evaluate the relationship between the C3/C4 ratio and other clinical-pathological factors.Propensity score matching and a multivariate Cox regression model were used to calculate the risk factors of renal outcome.Results:The median follow-up period was 75 months.During the follow-up period,62 patients(15.6%)developed into the endstage renal disease(ESRD).The C3/C4 ratio at baseline was associated with the level of serum creatinine(SCr),24 h urinary protein excretion(24 h Upre),global glomerular sclerosis,and tubulointerstitial lesion.The level of SCr and 24 h Upre and the degree of chronic kidney injury were statistically different among groups defined by different C3/C4 ratio levels.The survival rates of patients among groups with different C3/C4 ratio levels were different.After propensity score matching,eighty-eight pairs of patients were successfully matched,and the C3/C4 ratio was an influencing factor for the patients5 outcome(hazard ratio 0.587,95%confidence interval 0.329-0.880).Patients with a C3/C4 ratio<3.6 had a poorer outcome compared with the others(P=0.002).Conclusions:IgAN patients with decreased C3/C4 ratio displayed significantly more severe clinical symptoms and chronic renal injury than patients with higher ratios.A low C3/C4 ratio could be a risk factor for patients developing to ESRD.展开更多
基金financially supported by the National Science and Technology Major Project of China for Water Pollution Control and Treatment(No.2013ZX07202-010)。
文摘MnO2-impregnated carbon-coated Fe3 O4(Fe3O4/C/MnO2)nanocomposites with a good core-shell structure were prepared by direct oxidation of carbon-coated Fe3 O4(Fe3O4/C)microspheres with KMnO4 in alkaline solution and applied to adsorb bovine serum albumin(BSA).X-ray diffraction(XRD),transmission electron microscope(TEM),Fourier transform infrared spectrometer(FTIR),vibrating sample magnetometer(VSM)and thermogravimetric analyzer(TGA)tests show that Fe3O4/C microspheres were newly functionalized via the oxidation by KMnO4.Fe3O4/C/MnO2 nanocomposites exhibit a higher adsorption capacity for BSA than Fe3O4/C microspheres and the maximum adsorption of BSA on them occurs at pH 4.7,which is the isoelectric point of BSA.Langmuir isotherm model describes the adsorption of BSA better than Freundlich model and Temkin model,and the kinetics data fit well with the pseudo-second-order model.
文摘AIM: To help clarifying the possibility of connective-tissue diseases in men with penile or testicular prostheses. METHODS: Eight patients underwent inflatable penile prostheses and 15, testicular prostheses consented to the study. Their medical records were reviewed and a follow-up interview and physical and serological examinations were performed. RESULTS: In patients with penile prostheses, there was no abnormal antinuclear antibody (ANA) or IgM elevation. The serum levels of the rheumatoid factor (RF), C4, IgA and IgG were abnormal in one patient, and the levels of erythrocyte sedimentation rate (ESR) and C3, abnormal in two. Four had elevated IgE. In patients with testicular prostheses, there was no abnormal RF, ANA or IgM. The serum levels of ESR and IgA were abnormal in two, and three had abnormal C4, ten abnormal C3, and eleven decreased IgG. All had increased IgE. Men with penile prostheses had higher serum levels of IgG and IgM than those with testicular prostheses (P=0.001, P=0.016, respectively). The rates of abnormal values of IgE and IgG were higher in men with testicular prostheses than in men with penile prostheses (P=0.008, P=0.009, respectively). Physical examination was normal in all patients and nobody had documented symptoms pertinent to connective-tissue diseases. CONCLUSION: Our findings suggest that the risk of connective-tissue diseases is not higher in patients wearing prostheses as the ANA is negative and there is no apparent manifestation suggestive of connective-tissue diseases.
文摘Using isoelective focusing in immobilized pH gradients and immunoblot, C3 phenotypes (F, FS, S) and C3 HAV4-1 monoclonal (F±S±) phenotypes were performed in 90 patients with IgA glomerulonephrits,(G.N.).including 49 IgA G. N.hypertensive (H.T.) patients and 41 IgA G. N. normotensive (N.T.) patients, and in 224 normal subjects (N.S.). A significant difference of C3 phenotype distribution between both IgA G. N.(hypertensive and normotensive) and N. S. was .found (P<0.01,P<0.01respectively).In monoclonal C3 HAV4-1(±) distribution significant difference between IgA H. T.and N.S.was observed (P<0.01). Furthermore, F and S allele .frequency of IgA G. N. including HT and NT is significantly. different (P<0.05). This data suggests that hypertensive patients with IgA G. N. seems to be related io the abnormal C3 genetic factors and if this gene distributions can be used as a predictor for the prognosis still needs futher investigations.
基金This work was supported by grants from the Science and Technology Project of Beijing China(No.D181100000118004,No.D181100000118002).
文摘Background:Aberrant activation of the complement system plays an important role in the pathogenesis and development of immunoglobulin A nephropathy(IgAN).The relationship between serum complement and the clinical-histopathological features and outcomes of IgAN is controversial.This retrospective study aimed to examine the relationship between the complement 3/4(C3/C4)ratio and the clinicopathologic changes and prognosis of patients with IgAN.Methods:A total of 397 patients with primary IgAN from January 2007 to December 2012 at the Chinese People's Liberation Army General Hospital were included in this study.The correlation test and Chi-square test or one-way analysis of variance test were performed to evaluate the relationship between the C3/C4 ratio and other clinical-pathological factors.Propensity score matching and a multivariate Cox regression model were used to calculate the risk factors of renal outcome.Results:The median follow-up period was 75 months.During the follow-up period,62 patients(15.6%)developed into the endstage renal disease(ESRD).The C3/C4 ratio at baseline was associated with the level of serum creatinine(SCr),24 h urinary protein excretion(24 h Upre),global glomerular sclerosis,and tubulointerstitial lesion.The level of SCr and 24 h Upre and the degree of chronic kidney injury were statistically different among groups defined by different C3/C4 ratio levels.The survival rates of patients among groups with different C3/C4 ratio levels were different.After propensity score matching,eighty-eight pairs of patients were successfully matched,and the C3/C4 ratio was an influencing factor for the patients5 outcome(hazard ratio 0.587,95%confidence interval 0.329-0.880).Patients with a C3/C4 ratio<3.6 had a poorer outcome compared with the others(P=0.002).Conclusions:IgAN patients with decreased C3/C4 ratio displayed significantly more severe clinical symptoms and chronic renal injury than patients with higher ratios.A low C3/C4 ratio could be a risk factor for patients developing to ESRD.
