Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.mar...Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.marcescens infections and a rational selection of antibiotics.Methods:S.marcescens isolates were collected from 2014 to 2020,and the trend of detection and antimicrobial resistance were analyzed according to different types of infection.Results:For 7 consecutive years,the data showed that patients with S.marcescens infections were mainly from the intensive care unit(ICU)(384 isolates,40.98%),and the isolates recovered were mainly from sputum samples(743 isolates,79.30%).The number of isolated strains increased every year,and the average rate of detection ranged from 0.60%to 0.80%.The detection rate of S.marcescens with hospital-acquired infections(HAI)showed a downward trend and that of S.marcescens with colonization showed an upward trend.The detection rate of multidrug-resistant S.marcescens fluctuated between 8.33%–16.89%.The resistance rate of S.marcescens to piperacillin was 17.0%–29.06%and the resistance rate to piperacillin tazobactam was 2.95%–13.13%.For cephalosporin antibiotics,the resistance rates of S.marcescens to cefuroxime and cefazolin were>99%and the resistance rates to ceftazidime and cefepime were<13%.The resistance rate of S.marcescens to aminoglycoside antibiotics,especially amikacin,was the lowest.The resistance rate of S.marcescens with community-acquired infections(CAI)to carbapenems was higher than that with HAI and colonization.Conclusion:The different infection types of S.marcescens have different detection and epidemic trends.In addition,resistance to carbapenems is different across the strains.展开更多
以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结...以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.展开更多
Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care unit...Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.展开更多
We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergenc...We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.展开更多
基金Hospital Management Innovation Program of Jiangsu Hospital Association in 2021(Project No.:JSYGY-3-2021-511)。
文摘Objective:To explore the trend of detection and antimicrobial resistance of Serratia marcescens with different infection types for 7 consecutive years,to provide a reference for future studies for the control of S.marcescens infections and a rational selection of antibiotics.Methods:S.marcescens isolates were collected from 2014 to 2020,and the trend of detection and antimicrobial resistance were analyzed according to different types of infection.Results:For 7 consecutive years,the data showed that patients with S.marcescens infections were mainly from the intensive care unit(ICU)(384 isolates,40.98%),and the isolates recovered were mainly from sputum samples(743 isolates,79.30%).The number of isolated strains increased every year,and the average rate of detection ranged from 0.60%to 0.80%.The detection rate of S.marcescens with hospital-acquired infections(HAI)showed a downward trend and that of S.marcescens with colonization showed an upward trend.The detection rate of multidrug-resistant S.marcescens fluctuated between 8.33%–16.89%.The resistance rate of S.marcescens to piperacillin was 17.0%–29.06%and the resistance rate to piperacillin tazobactam was 2.95%–13.13%.For cephalosporin antibiotics,the resistance rates of S.marcescens to cefuroxime and cefazolin were>99%and the resistance rates to ceftazidime and cefepime were<13%.The resistance rate of S.marcescens to aminoglycoside antibiotics,especially amikacin,was the lowest.The resistance rate of S.marcescens with community-acquired infections(CAI)to carbapenems was higher than that with HAI and colonization.Conclusion:The different infection types of S.marcescens have different detection and epidemic trends.In addition,resistance to carbapenems is different across the strains.
文摘以耐铬(VI)菌株Serratia sp. CM01为研究对象,探究其全基因组信息,挖掘其潜在的铬代谢相关基因。本研究采用基因组测序技术对CM01进行全基因组测序并分析其基因序列特征;同时,结合前期差异蛋白研究结果,进行铬代谢相关基因分析。测序结果表明,CM01基因组大小为4,902,254 bp,预测编码蛋白序列的基因有4547个;蛋白功能注释结果显示其涉及氧化还原、氨基酸代谢、碳水化合物和能量代谢编码的基因有较高的占比。结合前期蛋白组学的结果,筛选出了12个与铬代谢相关的基因。qRT-PCR分析结果显示,在Cr(VI)胁迫下,ChrA1、Srpc、GrxA和NemA基因的表达上调。CM01基因组全序列已上传至NCBI,序列号:PRJNA675313。本研究通过对CM01的基因组序列分析,为全面了解细菌的铬代谢机制提供基础,为修复环境铬污染的新生物技术提供理论依据。The study focused on the chromium (VI)-resistant bacterial strain Serratia sp. CM01 to explore its whole-genome information and to mine its potential chromium metabolism-related genes. In this research, genomic sequencing technology was employed to perform whole-genome sequencing on CM01 and to analyze its gene sequence characteristics. Additionally, the results from previous differential protein studies were integrated to analyze genes related to chromium metabolism. The sequencing results indicated that the CM01 genome is 4,902,254 base pairs in size, with 4547 genes predicted to encode protein sequences. Protein function annotation revealed a high proportion of genes involved in oxidation-reduction, amino acid metabolism, carbohydrate metabolism, and energy metabolism. Combining the outcomes from previous proteomics studies, 12 genes related to chromium metabolism were identified. Quantitative real-time PCR analysis showed that the expression of ChrA1, Srpc, GrxA, and NemA genes was upregulated under Cr(VI) stress. The complete genome sequence of CM01 has been uploaded to NCBI with the accession number PRJNA675313. Through the genomic sequence analysis of CM01, this study provides a foundation for a comprehensive understanding of bacterial chromium metabolism mechanisms and offers a theoretical basis for the development of new biotechnologies for the remediation of environmental chromium pollution.
文摘Serratia is an opportunistic pathogenic bacterium of the Enterobacteriaceae family, occasionally responsible for epidemics of nosocomial infections in critical departments;in particular in neonatal intensive care units Enterobacteriaceae form a large family of Gram-negative bacteria, which cause diseases of highly variable severity, due to distinct pathogenic mechanisms. This family is heterogeneous as it consists of about 30 genera of bacteria and more than 100 species. However, all these germs have in common their preferential location in the digestive system, some being part of the normal flora although they are also present in the environment. Several metabolic processes characterize this bacterial family. These include the ability to reduce nitrate to nitrite (for energy generation), ferment glucose, lack cytochrome oxidase, be aerobic or anaerobic, motile or immobile, … Enterobacteriaceae constitute more than 80% of the germs isolated in the laboratory: Escherichia, Salmonella, Shigella, Klebsiella, Enterobacter, Serratia, Proteus, Morganella and Yersinia are the rods most often found. Regarding nosocomial infection to Serratia, this bacterium colonizes the respiratory, digestive and urinary systems of patients, mainly responsible for bacteremia, infections of the lower respiratory tract, urinary and skin infections. Through our work, we report the epidemic experienced in the neonatal intensive care unit MOHAMMED VI university hospital, mother-child hospital MARRAKECH MOROCCO for three months from December at February 2023 interesting 30 newborns whose clinical presentation was different, the positive diagnosis was based on blood and geographical samples taken by the bacteriology department to isolate the offending germs, the therapeutic management of our patients consisted essentially of various hygiene measures in association with dual antibiotic therapy based on meropenem and amikacin. The evolution was marked by the death of 21 patients (70%) and the recovery of 9 patients (30%). The prognosis of infection by serratia remains bleak with high morbidity interest in prevention by respecting the rules of hygiene, which begins with hand disinfection.
文摘We present the 3<sup>rd</sup> known case of a Serratia rubidaea urinary tract infection in a 49-year-old male with past medical history of bilateral lower extremity paraplegia who presented to the emergency room with fever, lethargy, and red tinted urine. The Serratia genus, of the family Enterobacteracia, in particular the species marcescens, are important causes of infection in humans, animals, and insects, however, until the mid-1950s, this was not the case, and the organism was considered non-pathogenic and frequently used in medical experiments. Serratia are facultatively anaerobic, gram-negative rods, the majority with peritrichous flagella primarily inhabiting soil, water, and plant surfaces. Serratia marcescens is now a known pathogen, however, less frequently isolated species, including Serratia rubidaea, are worthy of discussion, especially due to its characteristic red color and rarity. We aim to increase the awareness Serratia rubidaea including its presentation, inherent antimicrobial resistance, and treatment options.
