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牙龈卟啉单胞菌磷酸酶SerB的表达、纯化和晶体衍射
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作者 高洁 杨金敏 +5 位作者 杨孟钰 王艳秋 张修月 葛燕 刘彬 吴桃清 《生物技术》 2025年第1期14-19,共6页
[目的]原核表达和纯化牙龈卟啉单胞菌SerB蛋白,并进行晶体筛选和X射线衍射。[方法]以生物信息学手段预测SerB蛋白基本特点,借助无缝克隆技术构建pET22b-serB重组质粒,并在大肠杆菌BL21(DE3)中表达,采用镍柱亲和层析和分子排阻层析纯化重... [目的]原核表达和纯化牙龈卟啉单胞菌SerB蛋白,并进行晶体筛选和X射线衍射。[方法]以生物信息学手段预测SerB蛋白基本特点,借助无缝克隆技术构建pET22b-serB重组质粒,并在大肠杆菌BL21(DE3)中表达,采用镍柱亲和层析和分子排阻层析纯化重组SerB蛋白,孔雀绿显色法检测磷酸酶活性,以及筛选蛋白结晶条件并收集X射线衍射数据。[结果]SerB蛋白由N端的ACT结构域和C端的丝氨酸磷酸酶结构域组成,分子量为45.93 kDa。成功构建pET22b-serB重组质粒及获得分子量正确、浓度为6 mg/mL的目的蛋白。孔雀绿显色法显示SerB具有丝氨酸磷酸酶活性。在0.1 mol/L C_(3)H_(2)Na_(2)O_(4)、pH 8.0,0.1 mol/L Tris-HCl pH 8.0,30%(W/V)PEG1500条件下获得了较好的SerB晶体,分辨率为3.84A。[结论]成功表达具有丝氨酸磷酸酶活性的牙龈卟啉单胞菌SerB蛋白并结晶,收集到分辨率为3.84?的衍射数据。 展开更多
关键词 牙龈卟啉单胞菌 SerB 磷酸酶 诱导表达 纯化 酶活检测 晶体 X射线衍射
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Sanjak Bosniak Volunteers in the Turkish Military Orders From Galipoli to Galicia (1916-1918)
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作者 Redzep Skrijelj 《International Relations and Diplomacy》 2014年第11期775-782,共8页
The emergence of World War I (1914) brought the occupation of Novi Pazar by Austria-Hungary (19 of November 1915) which resulted in epidemics, famine and destitution among its inhabitants. Despite of the attenuati... The emergence of World War I (1914) brought the occupation of Novi Pazar by Austria-Hungary (19 of November 1915) which resulted in epidemics, famine and destitution among its inhabitants. Despite of the attenuation of the population structure, Austria-Hungary made a strong pressure on the Muslim population of Sanjak by representing the ongoing worldwide conflict (World War I) as a "holy war" which aimed the destruction of the Ottomans and Islam by its enemies. By using the slogans "For creed and faith" and "Everyone Turkish must check into the Volunteers" during 1916, with the help of local agas and beys, a couple of thousand males at the ages between 18 and 50 were gathered and dispersed among the frontlines spreading from Arabia to Galicia. Thus, a large number of Muslims applied to "Durumlije" and joined the struggle because of the Ottoman Empire had been an ally and fought together with the Axis Powers (Germany and Austria-Hungary) against Russia and other Allied (Entente) Armies. "Durumlije", among their Austria-Hungarian military uniforms wore a crescent-star bandage alongside a tarboosh on their heads. They were being brought to the Galician frontline after a very short period of military instructions which never lasted longer than a month. A smaller number of them were sent to the frontlines towards Arabia, while some of them lost their lives. Part became prisoners of war and afterwards most of them continued their lives in Turkey. Nevertheless, a small number of"Durumlije" managed to return to Sanjak. After succeeding to return their destinies were uncertain. After the conclusion of World War I they faced repression and terror because of their previous war engagement in the newly formed Kingdom of Serbs, Croats and Slovenes (1918). A large part of them were forced to flee to Turkey because of poverty and fear of retaliations. 展开更多
关键词 Durumlije (volunteers) Bosniaks ottomans Austria-Hungary GALICIA Galipoli ARABIA Kingdom of serbs Croats and Slovenes
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Interviewing on Sensitive Issues: Negotiating new Identities in a Collectively Traumatized Society
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作者 Cemre Aydoğan 《Cultural and Religious Studies》 2023年第9期407-419,共13页
In this article I aim to highlight the question how to negotiate and reconstruct new identities among previously perpetuator groups in the trauma zones by looking at a specific case example,Bosnia-Herzegovina(BiH)and ... In this article I aim to highlight the question how to negotiate and reconstruct new identities among previously perpetuator groups in the trauma zones by looking at a specific case example,Bosnia-Herzegovina(BiH)and Bosnian Serbs.After the end of the Bosnian war(1992-1995)through Dayton Peace Accord,Bosnian Serbs have been started to be classified as the perpetuator group of the war.However,both Bosniaks,the main victim ethnic group of the war,and Bosnian Serbs have also started to draw the borders of Serbness into different boundaries,such as moral and religious ones.In this study I employed the method of(online)in-depth interviewing with my Bosniak and Bosnian Serb participants to underline and display which components and experiences have been shaping Bosnian Serbness since the end of the war.After the analysis of my interviews,one could argue that burden of guilt and burden of loss might have caused re-imagination of Bosnian Serbness as today’s victims.Furthermore,according to the main pattern I detected among the testimonies of my participants,one could also argue that exclusion of Bosnian Serbs due to their ethnicity from everyday tasks or rituals makes their another supreme identity much more visible:they are Christian Orthodoxies of Bosnia now. 展开更多
关键词 collective trauma memory BOSNIA-HERZEGOVINA Bosnian serbs identity negotiation
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