Background Tanis was reported as a putative receptor for serum amyloid A （SAA） involving glucose regulated protein in insulin regulated resistance. It was found to be dysregulated in diabetic rats （Psammomys obesus...Background Tanis was reported as a putative receptor for serum amyloid A （SAA） involving glucose regulated protein in insulin regulated resistance. It was found to be dysregulated in diabetic rats （Psammomys obesus, Israeli sand rat） and its homologue for humans is SelS/AD-015. The present study analyzed mRNA expression of SelS in omental adipose tissue biopsies from patients with type 2 diabetes mellitus （T2DM）, and age- and weight-matched nondiabetic patients, the relationship of SelS mRNA with Homa-IR and serum SAA level. Methods Human omental adipose tissues from ten cases of type 2 diabetic patients and twelve cases of nondiabetic individuals were analyzed for the expression level of SelS mRNA by semiquantitative polymerase chain reaction （PCR）, Homa-IR estimated by standard formula and SAA level by enzyme-linked immunosorbent assay （ELISA）. Results SelS mRNA expression, Homa-IR and serum SAA were higher in T2DM sufferers than in nondiabetic control group. SelS mRNA level was positively correlated with Homa-IR and SAA level in each group. Conclusions SelS protein may be involved in insulin resistance in Chinese with T2DM by acting as the SAA receptor, thus playing an important role in the development of T2DM and atherosclerosis.展开更多
Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which h...Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which have been associated with multiple health-promoting properties(Azuma et al.,2008;Gurbuz et al.,2018).Given its significant hybrid vigor,F1 hybrid varieties are widely preferred in commercial cultivation(Mistry et al.,2018).However,traditional breeding practices predominantly rely on phenotypic selection,a process that is not only labor-intensive but also time-consuming.展开更多
社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观...社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观念、情感和行为模式。研究表明,社会情感能力不仅对个体的学业、事业发展和身心健康具有重要影响,还是减少反社会行为、促进社会和谐、提升幸福感的重要因素。初中生阶段是社会情感能力发展的关键阶段,本文以社会情感学习理论框架(SEL)为理论基础,在学校教育中对初中生社会情感能力的培养进行探索,并以心理课堂为例,以期为素质教育及学生非认知能力的培养提供理论基础。Social-emotional competence is a component of the core competence of middle school students facing the 21st century, which has an important impact on individual’s future achievement and mental health. Social affective ability learning is a comprehensive ability gradually formed by individuals through formal and informal learning, which is related to emotion management, communication and cooperation, effective cognition and achievement of goals, etc. It is manifested in the continuous development of ideas, emotions and behavior patterns. Studies have shown that social-emotional ability not only has an important impact on individual’s academic and career development and physical and mental health, but also an important factor to reduce anti-social behavior, promote social harmony and enhance happiness. The stage of junior high school students is the key stage of the development of social-emotional ability. Based on the theoretical framework of social-emotional learning (SEL), this paper explores the cultivation of junior high school students’ social-emotional ability in school education, and takes psychological classroom as an example, in order to provide a theoretical basis for quality education and the cultivation of students’ non-cognitive ability.展开更多
BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitorin...BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitoring protein synthesis and processing unfolded or misfolded proteins in actively growing tumor cells.However,the role of the multiple ERAD complexes in liver cancer remains unclear.AIM To elucidate the effects of SEL1L-mediated ERAD on Huh7 and explore the underlying mechanisms in vivo and in vitro.METHODS Huh7 cells were treated with ERAD inhibitor to identify ERAD’s role.Cell counting kit-8,5-ethynyl-2’-deoxyuridine and colony formation experiments were performed.Apoptosis level and migration ability were assessed using fluorescence activated cell sorting and Transwell assay,respectively.Huh7 SEL1L knockout cell line was established via clustered regularly interspaced short palindromic repeats,proliferation,apoptosis,and migration were assessed through previous experiments.The role of SEL1L in vivo and the downstream target of SEL1L were identified using Xenograft and mass spectrometry,respectively.RESULTS The ERAD inhibitor suppressed cell proliferation and migration and promoted apoptosis.SEL1L-HRD1 significantly influenced Huh7 cell growth.SEL1L knockout suppressed tumor cell proliferation and migration and enhanced apoptosis.Mass spectrometry revealed EXT2 is a primary substrate of ERAD.SEL1L knockout significantly increased the protein expression of EXT2.Furthermore,EXT2 knockdown partially restored the effect of SEL1L knockout.CONCLUSION ERAD inhibition suppressed the proliferation and migration of Huh7 and promoted its apoptosis.EXT2 plays an important role and ERAD might be a potential treatment for Huh7 hepatocellular carcinoma.展开更多
社会情感学习(Social and Emotional Learning,简称SEL),作为一种教育理念,近年来在全球教育领域受到广泛关注。该理念认为,社交和情感学习是获得识别和管理情绪、培养对他人的同情心、建立积极关系、决策力和有效处理具有挑战性的情况...社会情感学习(Social and Emotional Learning,简称SEL),作为一种教育理念,近年来在全球教育领域受到广泛关注。该理念认为,社交和情感学习是获得识别和管理情绪、培养对他人的同情心、建立积极关系、决策力和有效处理具有挑战性的情况等能力的过程。1994年,美国学术、社会和情感学习合作组织(Collaborative for Academic,Social and Emotional Learning)提出了社会情感学习框架,将社会情感能力分为自我意识(Self-awareness)、自我管理(Self-management)、社会意识(Social awareness)、关系技能(Relationship-skills)和负责任的决策(Responsible decision-making)五个维度,这是目前全球范围内最具影响力并被广泛采纳的SEL框架。展开更多
本研究立足于核心素养教育的时代背景,聚焦社会情感学习(Social and Emotional Learning,SEL)在基础教育阶段与音乐教育的深层关联。新课标教改背景下音乐学科作为美育的重要载体,其培养目标与SEL呈现出高度的关联性。音乐教育特有的审...本研究立足于核心素养教育的时代背景,聚焦社会情感学习(Social and Emotional Learning,SEL)在基础教育阶段与音乐教育的深层关联。新课标教改背景下音乐学科作为美育的重要载体,其培养目标与SEL呈现出高度的关联性。音乐教育特有的审美体验性、情感表达性和社会互动性特征使其成为实现SEL培养目标的理想场域。本文就核心素养的内涵与社会情感学习能力的关联性展开分析,在此基础上开拓中小学音乐教学的实践路径,为学校教育中SEL的本土化实施提供实践灵感,同时为中小学音乐教师提供更多教学研究思路。展开更多
基金This study was supported by grants from National Natural Science Foundation of China （No. 30670649, No. 30470682）, National Key Research Project for the Tenth Five Year Plan （No. 2001BA702B01）, and Key Research Project of Liaoning Province Bureau of Science and Technology （No. 2002225003-6）.
文摘Background Tanis was reported as a putative receptor for serum amyloid A （SAA） involving glucose regulated protein in insulin regulated resistance. It was found to be dysregulated in diabetic rats （Psammomys obesus, Israeli sand rat） and its homologue for humans is SelS/AD-015. The present study analyzed mRNA expression of SelS in omental adipose tissue biopsies from patients with type 2 diabetes mellitus （T2DM）, and age- and weight-matched nondiabetic patients, the relationship of SelS mRNA with Homa-IR and serum SAA level. Methods Human omental adipose tissues from ten cases of type 2 diabetic patients and twelve cases of nondiabetic individuals were analyzed for the expression level of SelS mRNA by semiquantitative polymerase chain reaction （PCR）, Homa-IR estimated by standard formula and SAA level by enzyme-linked immunosorbent assay （ELISA）. Results SelS mRNA expression, Homa-IR and serum SAA were higher in T2DM sufferers than in nondiabetic control group. SelS mRNA level was positively correlated with Homa-IR and SAA level in each group. Conclusions SelS protein may be involved in insulin resistance in Chinese with T2DM by acting as the SAA receptor, thus playing an important role in the development of T2DM and atherosclerosis.
基金supported by Yuelushan Laboratory Breeding Program(Grant No.YLS-2025-ZY02013)The Project of National Key Laboratory for Tropical Crop Breeding(Grant No.NKLTCB202341)+4 种基金The New Variety Breeding Project of the Major Science and Technology Projects of Zhejiang(Grant No.2021C02065-1-3)Hunan Provincial Agricultural Science and Technology Innovation Fund Project(Grant No.2025CX115)Key R&D Projects in Hainan Province(Grant No.ZDYF2023XDNY041)Central Public-interest Scientific Institution Basal Research Fund(Grant No.1630062022003)2024 Sanya Technology Stars Program(Grant No.2024KJFX022).
文摘Eggplant(Solanum melongena L.)is a globally important vegetable crop,renowned for its nutritional value and economic significance.It is abundant in bioactive compounds such as anthocyanins and chlorogenic acid,which have been associated with multiple health-promoting properties(Azuma et al.,2008;Gurbuz et al.,2018).Given its significant hybrid vigor,F1 hybrid varieties are widely preferred in commercial cultivation(Mistry et al.,2018).However,traditional breeding practices predominantly rely on phenotypic selection,a process that is not only labor-intensive but also time-consuming.
文摘社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观念、情感和行为模式。研究表明,社会情感能力不仅对个体的学业、事业发展和身心健康具有重要影响,还是减少反社会行为、促进社会和谐、提升幸福感的重要因素。初中生阶段是社会情感能力发展的关键阶段,本文以社会情感学习理论框架(SEL)为理论基础,在学校教育中对初中生社会情感能力的培养进行探索,并以心理课堂为例,以期为素质教育及学生非认知能力的培养提供理论基础。Social-emotional competence is a component of the core competence of middle school students facing the 21st century, which has an important impact on individual’s future achievement and mental health. Social affective ability learning is a comprehensive ability gradually formed by individuals through formal and informal learning, which is related to emotion management, communication and cooperation, effective cognition and achievement of goals, etc. It is manifested in the continuous development of ideas, emotions and behavior patterns. Studies have shown that social-emotional ability not only has an important impact on individual’s academic and career development and physical and mental health, but also an important factor to reduce anti-social behavior, promote social harmony and enhance happiness. The stage of junior high school students is the key stage of the development of social-emotional ability. Based on the theoretical framework of social-emotional learning (SEL), this paper explores the cultivation of junior high school students’ social-emotional ability in school education, and takes psychological classroom as an example, in order to provide a theoretical basis for quality education and the cultivation of students’ non-cognitive ability.
基金Supported by the National Natural Science Foundation of China,No.82241219,No.82127808 and No.81921004The Shenzhen Science and Technology Program,No.JCYJ20210324120813037.
文摘BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitoring protein synthesis and processing unfolded or misfolded proteins in actively growing tumor cells.However,the role of the multiple ERAD complexes in liver cancer remains unclear.AIM To elucidate the effects of SEL1L-mediated ERAD on Huh7 and explore the underlying mechanisms in vivo and in vitro.METHODS Huh7 cells were treated with ERAD inhibitor to identify ERAD’s role.Cell counting kit-8,5-ethynyl-2’-deoxyuridine and colony formation experiments were performed.Apoptosis level and migration ability were assessed using fluorescence activated cell sorting and Transwell assay,respectively.Huh7 SEL1L knockout cell line was established via clustered regularly interspaced short palindromic repeats,proliferation,apoptosis,and migration were assessed through previous experiments.The role of SEL1L in vivo and the downstream target of SEL1L were identified using Xenograft and mass spectrometry,respectively.RESULTS The ERAD inhibitor suppressed cell proliferation and migration and promoted apoptosis.SEL1L-HRD1 significantly influenced Huh7 cell growth.SEL1L knockout suppressed tumor cell proliferation and migration and enhanced apoptosis.Mass spectrometry revealed EXT2 is a primary substrate of ERAD.SEL1L knockout significantly increased the protein expression of EXT2.Furthermore,EXT2 knockdown partially restored the effect of SEL1L knockout.CONCLUSION ERAD inhibition suppressed the proliferation and migration of Huh7 and promoted its apoptosis.EXT2 plays an important role and ERAD might be a potential treatment for Huh7 hepatocellular carcinoma.
文摘社会情感学习(Social and Emotional Learning,简称SEL),作为一种教育理念,近年来在全球教育领域受到广泛关注。该理念认为,社交和情感学习是获得识别和管理情绪、培养对他人的同情心、建立积极关系、决策力和有效处理具有挑战性的情况等能力的过程。1994年,美国学术、社会和情感学习合作组织(Collaborative for Academic,Social and Emotional Learning)提出了社会情感学习框架,将社会情感能力分为自我意识(Self-awareness)、自我管理(Self-management)、社会意识(Social awareness)、关系技能(Relationship-skills)和负责任的决策(Responsible decision-making)五个维度,这是目前全球范围内最具影响力并被广泛采纳的SEL框架。
文摘本研究立足于核心素养教育的时代背景,聚焦社会情感学习(Social and Emotional Learning,SEL)在基础教育阶段与音乐教育的深层关联。新课标教改背景下音乐学科作为美育的重要载体,其培养目标与SEL呈现出高度的关联性。音乐教育特有的审美体验性、情感表达性和社会互动性特征使其成为实现SEL培养目标的理想场域。本文就核心素养的内涵与社会情感学习能力的关联性展开分析,在此基础上开拓中小学音乐教学的实践路径,为学校教育中SEL的本土化实施提供实践灵感,同时为中小学音乐教师提供更多教学研究思路。
