Selenolate ligands are expected to endow fluorescent gold nanoclusters(AuNCs)with better stability and more bioactivity than thiolate ligands,making them promising in the biological field.However,there are few studies...Selenolate ligands are expected to endow fluorescent gold nanoclusters(AuNCs)with better stability and more bioactivity than thiolate ligands,making them promising in the biological field.However,there are few studies on the synthesis of water-soluble selenolate-protected AuNCs,and the impact of selenolate ligands on the optical properties of AuNCs is still unclear.In this study,we synthesized selenolatecostabilized water-soluble,near-infrared fluorescent AuNCs with four different amounts of benzeneselenol(PhSeH),and systematically investigated the role of PhSeH on their optical properties.It is discovered that an appropriate PhSeH content is favorable for the fluorescence enhancement of AuNCs due to the ligand to metal charge transfer effect.Moreover,AuNCs co-stabilized by selenolate ligands exhibit better photostability and long-term stability compared with AuNCs stabilized by thiolate ligands,owing to the introduction of Au-Se bond on their surfaces.Further cellular experiments revealed that selenolate ligands can also affect the cellular uptake efficiency of AuNCs and their imaging property.These results provide important knowledges for further development of new,robust selenolate-stabilized metal NCs for biological application.展开更多
A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is wel...A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.展开更多
基金support from the National Natural Science Foundation of China(No.22274131)Shaanxi Fundamental Science Research Project for Chemistry&Biology(No.22JHQ071)。
文摘Selenolate ligands are expected to endow fluorescent gold nanoclusters(AuNCs)with better stability and more bioactivity than thiolate ligands,making them promising in the biological field.However,there are few studies on the synthesis of water-soluble selenolate-protected AuNCs,and the impact of selenolate ligands on the optical properties of AuNCs is still unclear.In this study,we synthesized selenolatecostabilized water-soluble,near-infrared fluorescent AuNCs with four different amounts of benzeneselenol(PhSeH),and systematically investigated the role of PhSeH on their optical properties.It is discovered that an appropriate PhSeH content is favorable for the fluorescence enhancement of AuNCs due to the ligand to metal charge transfer effect.Moreover,AuNCs co-stabilized by selenolate ligands exhibit better photostability and long-term stability compared with AuNCs stabilized by thiolate ligands,owing to the introduction of Au-Se bond on their surfaces.Further cellular experiments revealed that selenolate ligands can also affect the cellular uptake efficiency of AuNCs and their imaging property.These results provide important knowledges for further development of new,robust selenolate-stabilized metal NCs for biological application.
基金supported by the National Natural Science Foundation of China (Nos. 21622504, 21302050)the Hunan Provincial Natural Science Foundation of China (Nos. 14JJ2047)+1 种基金the Hunan University Fund for Multidisciplinary Developing (Nos. 2015JCA04)Open Funding Project of the State Key Laboratory of Bioreactor Engineering
文摘A new fluorescent probe（Rhod-Sec） for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.
