Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation a...Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.展开更多
The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also ...The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.展开更多
High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched gree...High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched green tea(Se-GT)and ordinary green tea(GT)on prevention of hypertension of rats induced by high-salt diet,as well as their potential regulatory and mechanism.Our results showed that GT and Se-GT supplementations significantly prevented the increase of blood pressure(BP),activated the phosphoinosmde-3-kinase/protein kinase B(PI3K/Akt)signaling pathway,and regulated the gene expression related to BP,as well as improved the tissue damage like heart,liver,and kidneys.Besides,the key parameters associated with oxidative stress,inflammation and endothelial dysfunction were also altered by GT and Se-GT treatments.Importantly,GT or Se-GT administration adjusted the diversity and composition of the intestinal flora.Moreover,GT and Se-GT supplementations increased the abundance of beneficial bacteria and reduced the abundance of harmful or conditional pathogenic bacteria.More specifically,GT intake specifically and significantly enriched the relative abundance of Paraprevotella and Bacteroides,whereas Se-GT was characterized by specific and significant enrichment for Allobaculum and Bifidobacterium.Our results proved that dietary supplement of GT and Se-GT remarkably improved the vascular functions and effectively prevented tissue damage by regulation of intestinal flora,and thus preventing hypertension induced by high-salt diet.展开更多
As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and probl...As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and problems of the selenium-enriched tea products of Ankang were analyzed in terms of capital,technology,management and sales mode.This research is of positive significance to the development of selenium-enriched products and other agricultural and sideline products of health value,and plays a very important role in promoting the cultivation of village-level characteristic industries and the effective realization of targeted poverty alleviation.展开更多
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium prot...[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.展开更多
Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eg...Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eggs,different selenium sources,additive doses,feeding time,and the addition of other vitamins,trace elements,and methionine in the diet will influence the deposition of selenium in eggs.Through this review,it is intended to provide a reference for the practical,safe and economical production of selenium-enriched eggs.展开更多
In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis ...In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis of Qinghai selenium-enriched soil selenium content and5 kinds of main vegetables corresponds to the total selenium,part of the mineral elements and soluble sugar and VC content,the effects of soil selenium levels on selenium absorption and nourishment quality of vegetables were researched. The results showed that he Qinghai selenium-enriched soil selenium content in the range of 100. 00-563. 00 μg/kg,among them,the soil samples with sufficient selenium and rich selenium accounted for 76. 53% and 18. 88%,respectively. Soil selenium content of garlic sampling area was relatively high,and soil selenium content of sugar beet sampling area was relatively low. Vegetable selenium content was in the range of 11. 00-340. 94 μg/kg,the average content of total selenium content of garlic was up to 170. 40 μg/kg,and the average content of the total selenium content of radish is 73. 00 μg/kg.90. 63% of the vegetables in the region reached the level of sufficient selenium,and 70. 31% reached the level of selenium enrichment. The average content of Ca and Mg was higher than the national average. There was no significant correlation between selenium content of soil and vegetable and nutritional quality.展开更多
Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and se...Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and selenium-enriched industry development in Guangxi,it discussed current practice,stressed green,ecological,characteristic demonstration,transformation and upgrading,and targeted poverty alleviation. Finally,it came up with perspective,practical,and operational strategies and recommendations for development of selenium-enriched industry,to provide reference for sustainable development of selenium-enriched functional industry in Guangxi.展开更多
Male infertility is a worldwide health problem,associated with the impediment of spermatogenesis.As a dualpurpose medicinal-edible oil crop,selenium-enriched rapeseed exhibits male reproductive benefits,yet its bioact...Male infertility is a worldwide health problem,associated with the impediment of spermatogenesis.As a dualpurpose medicinal-edible oil crop,selenium-enriched rapeseed exhibits male reproductive benefits,yet its bioactive components and underlying mechanisms remain unclear.In this study,the extraction procedure was optimized using response surface methodology(RSM).The crude extracts were further fractionated,and water extract(WE)was identified as the most bioactive fraction by bioassay-guided fractionation method.High performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS)analysis revealed methylselenocysteine(MeSeCys)as the predominant selenocompound within WE.Both MeSeCys and WE exhibited spermatogenic enhancement in vitro and in vivo.Mechanistic studies showed that SLC7A11/LRP8 mediated the transport of MeSeCys,while the spermatogenic activity of MeSeCys and WE was related to selenium-glutathione metabolic pathways.However,RNA-sequencing(RNA-seq),qRT-PCR,and WB analyses uncovered WE’s additional regulation of MAPK/ERK signaling,suggesting the presence of other bioactive components.Ultra-performance liquid-chromatography tandem mass spectrometry(UPLC-MS/MS)analysis showed 340 other constituents in WE.Through network pharmacology,computational screening,and cellular experiments,we identified sulforaphane as a potential bioactive compound in WE and demonstrated its synergistic effect with MeSeCys in promoting cell viability.Our findings would contribute to development of seleniumenriched rapeseed with high nutritive value.展开更多
Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and ...Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and discusses data on Se biofortification with different sources of supplementation,from inorganic to organic forms,with special focus on Se-enriched yeast(Se-yeast).Although Se-yeast remains one of the main sources of organic Se,other emerging and innovative sources are reviewed,such as Se-enriched insects and Se-nanoparticles and their potential use in animal nutrition.Se-enriched insects are discussed as an option for supplying Se in organic form to livestock diets.Se-nanoparticles are also discussed,as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms,compared to selenite and selenate.We also provide up to date information on the legal framework in the EU,USA,and Canada of Se that is contained in feed additives.From the scientific evidence available in the literature,it can be concluded that among the inorganic forms,sodium selenite is still one of the main options,whereas Se-yeast remains the primary organic form.However,other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.展开更多
In this work,the content of selenium(Se),physicochemical properties,and antioxidant capacity of Se-enriched peanut proteins isolate(S-PPI)and its fractions arachin(S-Arachin)and conarachin(S-Conarachin)were investigat...In this work,the content of selenium(Se),physicochemical properties,and antioxidant capacity of Se-enriched peanut proteins isolate(S-PPI)and its fractions arachin(S-Arachin)and conarachin(S-Conarachin)were investigated.The S-PPI,S-Arachin,and S-Conarachin were prepared using natural Se-enriched peanut with alkali extraction,acid precipitation,and sedimentation coefficients method.The Se content of S-PPI(420.83μg/kg)was two times compared to ordinary peanut protein isolate(O-PPI,203.10μg/kg).Besides,the Se is more likely to bind with conarachin than arachin.The solubility curves of these peanut proteins at different pH were similar.The emulsifying activities and the emulsion stability of S-Arachin was relatively low,as well as its foaming activity and the foam stability was the lowest.The DPPH scavenging activity of peanut proteins were decreased in order of S-Conarachin,S-PPI,S-Arachin,and O-PPI,as well as reducing power and the superoxide radical scavenging activity.The antioxidant activity trend was similar to Se content distribution trend,suggesting that the content of Se is related to antioxidant activity.The finding may indicate that Se content played a positive role in promoting the antioxidant activity of peanut protein.展开更多
Selenium-enriched exopolysaccharides(Se-EPS)are functional polysaccharides with promising immunomodu-latory,antitumor,and antioxidant properties that can be extracted by microbial selenium enrichment.The extracellular...Selenium-enriched exopolysaccharides(Se-EPS)are functional polysaccharides with promising immunomodu-latory,antitumor,and antioxidant properties that can be extracted by microbial selenium enrichment.The extracellular polysaccharides(EPSs)of Monascus purpureus possess various biological functions.However,sele-nite inhibits EPS production in M.purpureus,which limits its application.This study investigated the effects of daidzein on Se-EPS production,physicochemical properties,and in vitro antioxidant activity during selenium enrichment in M.purpureus.The results showed that at a daidzein concentration of 1 g/L,the yield of Se-EPS increased by 27.3%,the selenium content of Se-EPS increased by 62.1%,and its antioxidant capacity was significantly higher than that of the control group.Through transcriptomic analysis,we propose that daidzein enhances carbohydrate metabolism,antioxidant capacity,and cellular homeostasis in M.purpureus,providing a favorable environment for EPS synthesis and promoting EPS yield.Transcriptome analysis also revealed that 11 genes involved in EPS biosynthesis were significantly up-regulated,especially hexokinase(HK),glucose-6-phosphate isomerase(GPI)and mannose-6-phosphate isomerase(MPI).In addition,Glutathione reductase(GTR)and thioredoxin-disulfide reductase(TRR1)were significantly upregulated.The reduction of selenite to H_(2)Se and elemental Se(Se^(0))by glutathione and thioredoxin reduction systems provides precursors for Se-EPS synthesis.These findings advance the development and application of selenium-enriched M.purpureus and offer new insights into the synthesis of Se-EPS.展开更多
The control design, based on self-adaptive PID with genetic algorithms(GA) tuning on-line was investigated, for the temperature control of industrial microwave drying rotary device with the multi-layer(IMDRDWM) and wi...The control design, based on self-adaptive PID with genetic algorithms(GA) tuning on-line was investigated, for the temperature control of industrial microwave drying rotary device with the multi-layer(IMDRDWM) and with multivariable nonlinear interaction of microwave and materials. The conventional PID control strategy incorporated with optimization GA was put forward to maintain the optimum drying temperature in order to keep the moisture content below 1%, whose adaptation ability included the cost function of optimization GA according to the output change. Simulations on five different industrial process models and practical temperature process control system for selenium-enriched slag drying intensively by using IMDRDWM were carried out systematically, indicating the reliability and effectiveness of control design. The parameters of proposed control design are all on-line implemented without iterative predictive calculations, and the closed-loop system stability is guaranteed, which makes the developed scheme simpler in its synthesis and application, providing the practical guidelines for the control implementation and the parameter design.展开更多
[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of cr...[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of crops （tomato, leaf mustard, peanut, and carrot） were exogenous selenium fertilizer treated, to explore the selenium-enriching effects on these crops. [Results] Exogenous application of microbial nano-selenium can significantly increase the selenium content of the crops, of which the effects on big tomato （Weiyanuo） were the best. After spraying, the selenium content increased by 1.39 times, and at the same time the sugar content of tomato was also increased. [Conclusions] This study is expected to provide references for increase of the selenium content of agricultural products.展开更多
Selenium(Se)and glutathione(GSH)possess anti-inflammatory and antioxidant properties.Candida utilis has a high capacity for Se and GSH enrichment and it is beneficial to intestinal health;however,the impact of Se/GSH-...Selenium(Se)and glutathione(GSH)possess anti-inflammatory and antioxidant properties.Candida utilis has a high capacity for Se and GSH enrichment and it is beneficial to intestinal health;however,the impact of Se/GSH-enriched C.utilis on colitis is still not well understood.The present study aimed to assess the preventive and ameliorative effects of Se/GSH-enriched C.utilis SE-172 on colitis in mice.Selenized yeast effectively alleviated weight loss,inhibited colon shortening,reduced disease activity index,and decreased histological damage in mice with colitis.Supplementation with medium and high doses of selenized yeast significantly increased the activities of catalase,superoxide dismutase,and glutathione peroxidase,as well as interleukin-10 levels.Concurrently,it significantly decreased the levels of malondialdehyde and myeloperoxidase.Moreover,treatment with selenized yeast reduced apoptosis in colonic epithelial cells,downregulated the expression of interleukin-6,interleukin-1β,and interferon-gamma genes,and improved the immune response by modulating the proportions of innate immune cells.Selenized yeast also inhibited activation of the nuclear transcription factor-κB signaling pathway,attenuated intestinal barrier damage,and promoted the synthesis of short-chain fatty acids in the colon.Selenized C.utilis may thus have preventive and ameliorative effects on colitis by reducing the colonic inflammatory response and alleviating oxidative damage.These findings provide strong evidence for the development and application of Se/GSH-enriched C.utilis as a dietary treatment for colitis.展开更多
Selenium(Se)is an essential trace element for humans.Inorganic Se is converted into organic Se that is more easily absorbed during the growth of yeast.Patulin(PAT)is a fungal toxin that can cause damage to the intesti...Selenium(Se)is an essential trace element for humans.Inorganic Se is converted into organic Se that is more easily absorbed during the growth of yeast.Patulin(PAT)is a fungal toxin that can cause damage to the intestinal mucosa.Se can alleviate intestinal damage,and this study mainly focuses on the research of Se enrichment in Kluyveromyces marxianus YG-4(SeK)and its repair effect on jejunal injury induced by PAT.In this study,K.marxianus YG-4 could enrich up to 721.05μg/g of total Se content,of which 86.30%was organic Se.In the organic Se of SeK,protein-bound Se,polysaccharide-bound Se,and nucleic acid-bound Se accounted for 83.22%,10.76%,and 2.68%,respectively.Moreover,SeK had an increased amino acid content compared with K.marxianus YG-4.Finally,the morphology and survival ability of K.marxianus YG-4 were not disrupted after Se enrichment,indicating that SeK has the potential to become a probiotic in the food industry.Furthermore,SeK had the ability to alleviate jejunal damage induced by PAT.Specifically,SeK alleviated intestinal tissue lesions and led to an increase in mucin levels.Moreover,SeK promoted the expression of tight junction proteins(claudin-1,occludin,and ZO-1)and inhibited the activation of the NF-κB signaling pathway.Additionally,SeK reduced harmful bacteria including Turicibacter,Anaerotruncus,and Desulfovibrio,and increased beneficial bacteria including Eggerthellaceae,Muribaculaceae,Dubosiella,and short chain fatty acid producing bacteria(Erysipelotrichaceae and Faecalibaculum).In summary,SeK is expected to become a dietary candidate for alleviating the damage caused by mycotoxins.展开更多
基金supported by the National Natural Science Foundation of China(32302774)Beijing Innovation Consortium of Agriculture Research System(BAIC04)+1 种基金China Agriculture Research System(CARS-40)the Agricultural Science and Technology Innovation Program of the Feed Research Institute of the Chinese Academy of Agricultural Sciences(CAASIFR-ZDRW202402).
文摘Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.
基金National Natural Science Foundation of China(Nos.81872917 and 81741164)。
文摘The objective of this study was to verify the protective effect of Bifidobacterium longum(BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet(HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum(Se BL). C57 BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or Se BL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the m RNA levels of AMPK, PPAR-α and SREBP1 were invested. Se BL inhibited lipid accumulation in hepatocytes;reduced serum AST and ALT levels;improved dyslipidemia;decreased serum FFAs, TC, TG and LDL-C levels. Se BL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. Se BL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as Se BL, Se BL is more effective than BL. Se BL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.
基金financial sponsored by the National Key R&D Program of China(No.2018YFC1604405)Fund of Shanghai Engineering Research Center of Plant Germplasm Resources(No.17DZ2252700)Research on the health function of tea and deep-processed products in preventing metabolic diseases(No.C-6105-20-074)。
文摘High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched green tea(Se-GT)and ordinary green tea(GT)on prevention of hypertension of rats induced by high-salt diet,as well as their potential regulatory and mechanism.Our results showed that GT and Se-GT supplementations significantly prevented the increase of blood pressure(BP),activated the phosphoinosmde-3-kinase/protein kinase B(PI3K/Akt)signaling pathway,and regulated the gene expression related to BP,as well as improved the tissue damage like heart,liver,and kidneys.Besides,the key parameters associated with oxidative stress,inflammation and endothelial dysfunction were also altered by GT and Se-GT treatments.Importantly,GT or Se-GT administration adjusted the diversity and composition of the intestinal flora.Moreover,GT and Se-GT supplementations increased the abundance of beneficial bacteria and reduced the abundance of harmful or conditional pathogenic bacteria.More specifically,GT intake specifically and significantly enriched the relative abundance of Paraprevotella and Bacteroides,whereas Se-GT was characterized by specific and significant enrichment for Allobaculum and Bifidobacterium.Our results proved that dietary supplement of GT and Se-GT remarkably improved the vascular functions and effectively prevented tissue damage by regulation of intestinal flora,and thus preventing hypertension induced by high-salt diet.
基金National Key R&D Program of China(2018YFD1100202)Shaanxi Social Science Fund Program of China(2018S30)Shaanxi Undergraduate Innovation Planning Project of China(201829041).
文摘As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and problems of the selenium-enriched tea products of Ankang were analyzed in terms of capital,technology,management and sales mode.This research is of positive significance to the development of selenium-enriched products and other agricultural and sideline products of health value,and plays a very important role in promoting the cultivation of village-level characteristic industries and the effective realization of targeted poverty alleviation.
基金Supported by the Science and Technology Major Project of Guangxi(Guike AA17202026)Program for Scientific Research and Technology Development in Xixiangtant District of Nanning City(201710304)+7 种基金the Special Fund for the Innovation-Driven Development in Guangxi(Guike AA17202019-4&AA17202019)the Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(Guinongke 2017JM03)the Program for the Scientific Research and Technology Development in Guangxi(Guikehe415104001-22)the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences(Guinongke 2017YZ03)the Key Research and Development Program of Guangxi(Guike AB16380088)the Experiment Station for Selenium Featured Crops in Guangxi(Gui TS2016011)the Key Research and Development Program of Qingxiu District,Guangxi(2016039)the Scientific and Technological Transformative Project of Guangxi Academy of Agricultural Sciences(NO.2017NZ04)
文摘[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS（ atomic fluorescence spectrometry） respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.
文摘Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eggs,different selenium sources,additive doses,feeding time,and the addition of other vitamins,trace elements,and methionine in the diet will influence the deposition of selenium in eggs.Through this review,it is intended to provide a reference for the practical,safe and economical production of selenium-enriched eggs.
文摘In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis of Qinghai selenium-enriched soil selenium content and5 kinds of main vegetables corresponds to the total selenium,part of the mineral elements and soluble sugar and VC content,the effects of soil selenium levels on selenium absorption and nourishment quality of vegetables were researched. The results showed that he Qinghai selenium-enriched soil selenium content in the range of 100. 00-563. 00 μg/kg,among them,the soil samples with sufficient selenium and rich selenium accounted for 76. 53% and 18. 88%,respectively. Soil selenium content of garlic sampling area was relatively high,and soil selenium content of sugar beet sampling area was relatively low. Vegetable selenium content was in the range of 11. 00-340. 94 μg/kg,the average content of total selenium content of garlic was up to 170. 40 μg/kg,and the average content of the total selenium content of radish is 73. 00 μg/kg.90. 63% of the vegetables in the region reached the level of sufficient selenium,and 70. 31% reached the level of selenium enrichment. The average content of Ca and Mg was higher than the national average. There was no significant correlation between selenium content of soil and vegetable and nutritional quality.
基金Supported by Key Science and Technology Project for Innovation-driven Development of Guangxi"High Efficient and Safe Use of Selenium-enriched Soil Resources"(2017AA19015)Scientific Research and Technological Development Program Project of Guangxi Province(Gui Ke He 415104001-22)+2 种基金Key Research and Development Program of Guangxi(Gui Ke AB16380088 and Gui Ke AB16380164)Scientific Research and Technological Development Program Project of Nanning City(20152054-13)Scientific Research and Technological Development Program of Xixiangtang District of Nanning City(2015312)
文摘Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and selenium-enriched industry development in Guangxi,it discussed current practice,stressed green,ecological,characteristic demonstration,transformation and upgrading,and targeted poverty alleviation. Finally,it came up with perspective,practical,and operational strategies and recommendations for development of selenium-enriched industry,to provide reference for sustainable development of selenium-enriched functional industry in Guangxi.
基金supported by Central Public-interest Scientific Institution Basal Research Fund(1610172022007)Central Publicinterest Scientific Institution Basal Research Fund(Y2023XK04)the Talented Scientist Project of Qinghai Province(2023-NK-145).
文摘Male infertility is a worldwide health problem,associated with the impediment of spermatogenesis.As a dualpurpose medicinal-edible oil crop,selenium-enriched rapeseed exhibits male reproductive benefits,yet its bioactive components and underlying mechanisms remain unclear.In this study,the extraction procedure was optimized using response surface methodology(RSM).The crude extracts were further fractionated,and water extract(WE)was identified as the most bioactive fraction by bioassay-guided fractionation method.High performance liquid chromatography-inductively coupled plasma-mass spectrometry(HPLC-ICP-MS)analysis revealed methylselenocysteine(MeSeCys)as the predominant selenocompound within WE.Both MeSeCys and WE exhibited spermatogenic enhancement in vitro and in vivo.Mechanistic studies showed that SLC7A11/LRP8 mediated the transport of MeSeCys,while the spermatogenic activity of MeSeCys and WE was related to selenium-glutathione metabolic pathways.However,RNA-sequencing(RNA-seq),qRT-PCR,and WB analyses uncovered WE’s additional regulation of MAPK/ERK signaling,suggesting the presence of other bioactive components.Ultra-performance liquid-chromatography tandem mass spectrometry(UPLC-MS/MS)analysis showed 340 other constituents in WE.Through network pharmacology,computational screening,and cellular experiments,we identified sulforaphane as a potential bioactive compound in WE and demonstrated its synergistic effect with MeSeCys in promoting cell viability.Our findings would contribute to development of seleniumenriched rapeseed with high nutritive value.
基金"ASSO-Alimentazione Suina SOstenibile”funded by Regione Lombardia“One Health Action Hub:University Task Force for the resilience of territorial ecosystems”,funded by University of Milan-PSR 2021-GSA-Linea 6。
文摘Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis.This review surveys the role of Se in the environment,plants and animal bodies,and discusses data on Se biofortification with different sources of supplementation,from inorganic to organic forms,with special focus on Se-enriched yeast(Se-yeast).Although Se-yeast remains one of the main sources of organic Se,other emerging and innovative sources are reviewed,such as Se-enriched insects and Se-nanoparticles and their potential use in animal nutrition.Se-enriched insects are discussed as an option for supplying Se in organic form to livestock diets.Se-nanoparticles are also discussed,as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms,compared to selenite and selenate.We also provide up to date information on the legal framework in the EU,USA,and Canada of Se that is contained in feed additives.From the scientific evidence available in the literature,it can be concluded that among the inorganic forms,sodium selenite is still one of the main options,whereas Se-yeast remains the primary organic form.However,other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.
基金supported by the National Natural Science Foundation of China(31301436)Natural Science Fund for Distinguished Young Scholars of Jiangxi Province(20192BCB23006).
文摘In this work,the content of selenium(Se),physicochemical properties,and antioxidant capacity of Se-enriched peanut proteins isolate(S-PPI)and its fractions arachin(S-Arachin)and conarachin(S-Conarachin)were investigated.The S-PPI,S-Arachin,and S-Conarachin were prepared using natural Se-enriched peanut with alkali extraction,acid precipitation,and sedimentation coefficients method.The Se content of S-PPI(420.83μg/kg)was two times compared to ordinary peanut protein isolate(O-PPI,203.10μg/kg).Besides,the Se is more likely to bind with conarachin than arachin.The solubility curves of these peanut proteins at different pH were similar.The emulsifying activities and the emulsion stability of S-Arachin was relatively low,as well as its foaming activity and the foam stability was the lowest.The DPPH scavenging activity of peanut proteins were decreased in order of S-Conarachin,S-PPI,S-Arachin,and O-PPI,as well as reducing power and the superoxide radical scavenging activity.The antioxidant activity trend was similar to Se content distribution trend,suggesting that the content of Se is related to antioxidant activity.The finding may indicate that Se content played a positive role in promoting the antioxidant activity of peanut protein.
基金financially supported by the Natural Science Foun-dation of Jiangxi,China(20224ACB205011)the Research Project of State Key Laboratory of Food Science and Resources,Nanchang Uni-versity(project no.SKLF-ZZB202314).
文摘Selenium-enriched exopolysaccharides(Se-EPS)are functional polysaccharides with promising immunomodu-latory,antitumor,and antioxidant properties that can be extracted by microbial selenium enrichment.The extracellular polysaccharides(EPSs)of Monascus purpureus possess various biological functions.However,sele-nite inhibits EPS production in M.purpureus,which limits its application.This study investigated the effects of daidzein on Se-EPS production,physicochemical properties,and in vitro antioxidant activity during selenium enrichment in M.purpureus.The results showed that at a daidzein concentration of 1 g/L,the yield of Se-EPS increased by 27.3%,the selenium content of Se-EPS increased by 62.1%,and its antioxidant capacity was significantly higher than that of the control group.Through transcriptomic analysis,we propose that daidzein enhances carbohydrate metabolism,antioxidant capacity,and cellular homeostasis in M.purpureus,providing a favorable environment for EPS synthesis and promoting EPS yield.Transcriptome analysis also revealed that 11 genes involved in EPS biosynthesis were significantly up-regulated,especially hexokinase(HK),glucose-6-phosphate isomerase(GPI)and mannose-6-phosphate isomerase(MPI).In addition,Glutathione reductase(GTR)and thioredoxin-disulfide reductase(TRR1)were significantly upregulated.The reduction of selenite to H_(2)Se and elemental Se(Se^(0))by glutathione and thioredoxin reduction systems provides precursors for Se-EPS synthesis.These findings advance the development and application of selenium-enriched M.purpureus and offer new insights into the synthesis of Se-EPS.
基金Project(51090385) supported by the Major Program of National Natural Science Foundation of ChinaProject(2011IB001) supported by Yunnan Provincial Science and Technology Program,China+1 种基金Project(2012DFA70570) supported by the International Science & Technology Cooperation Program of ChinaProject(2011IA004) supported by the Yunnan Provincial International Cooperative Program,China
文摘The control design, based on self-adaptive PID with genetic algorithms(GA) tuning on-line was investigated, for the temperature control of industrial microwave drying rotary device with the multi-layer(IMDRDWM) and with multivariable nonlinear interaction of microwave and materials. The conventional PID control strategy incorporated with optimization GA was put forward to maintain the optimum drying temperature in order to keep the moisture content below 1%, whose adaptation ability included the cost function of optimization GA according to the output change. Simulations on five different industrial process models and practical temperature process control system for selenium-enriched slag drying intensively by using IMDRDWM were carried out systematically, indicating the reliability and effectiveness of control design. The parameters of proposed control design are all on-line implemented without iterative predictive calculations, and the closed-loop system stability is guaranteed, which makes the developed scheme simpler in its synthesis and application, providing the practical guidelines for the control implementation and the parameter design.
基金Supported by Program of Local Specialty Crop Varieties and Protective Cultivation Technique in Foshan City(Agricultural Technology Extension Project of Foshan City in 2017-2018)Characteristic Vegetable Innovation Team of Modern Agricultural Industrial Technology System of Guangdong Province(2016LM1015)
文摘[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of crops （tomato, leaf mustard, peanut, and carrot） were exogenous selenium fertilizer treated, to explore the selenium-enriching effects on these crops. [Results] Exogenous application of microbial nano-selenium can significantly increase the selenium content of the crops, of which the effects on big tomato （Weiyanuo） were the best. After spraying, the selenium content increased by 1.39 times, and at the same time the sugar content of tomato was also increased. [Conclusions] This study is expected to provide references for increase of the selenium content of agricultural products.
基金supported by the National Natural Science Foundation of China(22378284,21506136),and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Selenium(Se)and glutathione(GSH)possess anti-inflammatory and antioxidant properties.Candida utilis has a high capacity for Se and GSH enrichment and it is beneficial to intestinal health;however,the impact of Se/GSH-enriched C.utilis on colitis is still not well understood.The present study aimed to assess the preventive and ameliorative effects of Se/GSH-enriched C.utilis SE-172 on colitis in mice.Selenized yeast effectively alleviated weight loss,inhibited colon shortening,reduced disease activity index,and decreased histological damage in mice with colitis.Supplementation with medium and high doses of selenized yeast significantly increased the activities of catalase,superoxide dismutase,and glutathione peroxidase,as well as interleukin-10 levels.Concurrently,it significantly decreased the levels of malondialdehyde and myeloperoxidase.Moreover,treatment with selenized yeast reduced apoptosis in colonic epithelial cells,downregulated the expression of interleukin-6,interleukin-1β,and interferon-gamma genes,and improved the immune response by modulating the proportions of innate immune cells.Selenized yeast also inhibited activation of the nuclear transcription factor-κB signaling pathway,attenuated intestinal barrier damage,and promoted the synthesis of short-chain fatty acids in the colon.Selenized C.utilis may thus have preventive and ameliorative effects on colitis by reducing the colonic inflammatory response and alleviating oxidative damage.These findings provide strong evidence for the development and application of Se/GSH-enriched C.utilis as a dietary treatment for colitis.
基金supported by the key projects for tackling key core technologies in Shaanxi Province(2024-NC-GJHX-12)the Natural Science Foundation of China(32172301 and 32202065).
文摘Selenium(Se)is an essential trace element for humans.Inorganic Se is converted into organic Se that is more easily absorbed during the growth of yeast.Patulin(PAT)is a fungal toxin that can cause damage to the intestinal mucosa.Se can alleviate intestinal damage,and this study mainly focuses on the research of Se enrichment in Kluyveromyces marxianus YG-4(SeK)and its repair effect on jejunal injury induced by PAT.In this study,K.marxianus YG-4 could enrich up to 721.05μg/g of total Se content,of which 86.30%was organic Se.In the organic Se of SeK,protein-bound Se,polysaccharide-bound Se,and nucleic acid-bound Se accounted for 83.22%,10.76%,and 2.68%,respectively.Moreover,SeK had an increased amino acid content compared with K.marxianus YG-4.Finally,the morphology and survival ability of K.marxianus YG-4 were not disrupted after Se enrichment,indicating that SeK has the potential to become a probiotic in the food industry.Furthermore,SeK had the ability to alleviate jejunal damage induced by PAT.Specifically,SeK alleviated intestinal tissue lesions and led to an increase in mucin levels.Moreover,SeK promoted the expression of tight junction proteins(claudin-1,occludin,and ZO-1)and inhibited the activation of the NF-κB signaling pathway.Additionally,SeK reduced harmful bacteria including Turicibacter,Anaerotruncus,and Desulfovibrio,and increased beneficial bacteria including Eggerthellaceae,Muribaculaceae,Dubosiella,and short chain fatty acid producing bacteria(Erysipelotrichaceae and Faecalibaculum).In summary,SeK is expected to become a dietary candidate for alleviating the damage caused by mycotoxins.
