As one of the most important antioxidant enzymes, glutathione peroxidase（GPX） protects cells and tissues from oxidative damage, and plays an important role in cardiovascular and cerebrovascular injuries induced by o...As one of the most important antioxidant enzymes, glutathione peroxidase（GPX） protects cells and tissues from oxidative damage, and plays an important role in cardiovascular and cerebrovascular injuries induced by oxida- tive stress. The antioxidant effect of selenium-containing glutathione S-transferase（Se-GST）, a mimic of GPX was investigated on rat cardiomyocytes. To explore the protection function of Se-GST in hydrogen peroxide（H202） chal- lenged rat cardiomyocytes, we examined malondialdehyde（MDA）, lactate dehydrogenase（LDH）, superoxide dismu- tase（SOD） and cell apoptosis. The results demonstrate exposure of rat cardiomyocytes to H202 for 6 and 12 h induced the significant increases of MDA, LDH and apoptosis rate of cardiomyocytes, but pretreatment of rat cardiomyocytes with Se-GST at 0.0005 or 0.001 unit/mL prevents oxidative stress induced by H202 with the decreases of cell apopto- sis. All the results hint Se-GST has antioxidant activity for oxidative stress challenged rat cardiomyocvtes.展开更多
Diabetic wound healing impairment,a common complication of diabetes,has limited clinical treatment options and poor therapeutic outcomes,causing significant physical pain and psychological burden for patients.This stu...Diabetic wound healing impairment,a common complication of diabetes,has limited clinical treatment options and poor therapeutic outcomes,causing significant physical pain and psychological burden for patients.This study aims to accelerate wound healing by modulating cellular stress responses,offering a safe and efficient new therapeutic strategy.Herein,a seleniumcontaining polyurethane(SePU)thermo-sensitive hydrogel was synthesized,and its mechanism for promoting diabetic wound healing by activating the unfolded protein response(UPR)was elucidated.Hydroxybutyl chitosan(HBC)offers amore convenient application for SePU,with its high hydroxybutyl substitution enabling the hydrogel to undergo a rapid sol-gel transition at physiological temperatures.In vitro experiments showed that SePU thermo-sensitive hydrogel(SePU/HBC),at appropriate concentrations,significantly promoted the proliferation,spreading,migration,and adhesion of human skin fibroblasts(HSFs),while inhibiting inflammation.In vivo diabeticmouse model,SePU/HBC exhibited a significant wound-healing effect,promoting re-epithelialization,collagen formation and maturation.Mechanistic studies revealed that SePU/HBC alleviated endoplasmic reticulum stress under hyperglycemic conditions by activating the UPR-related gene ATF6 to alleviate endoplasmic reticulum stress(ERS)and inhibit apoptosis.This study offers a novel strategy for diabetic wound treatment using SePU/HBC,which activates the UPR and inhibits apoptosis,demonstrating promising clinical applications for wound healing.展开更多
Pyridylphosphonium salts,which are readily available and air and thermally stable,have been used to effectively synthesize structurally diverse pyridines.Herein,we report the pnictogen bonding(PnB)enabled photoactivat...Pyridylphosphonium salts,which are readily available and air and thermally stable,have been used to effectively synthesize structurally diverse pyridines.Herein,we report the pnictogen bonding(PnB)enabled photoactivation of pyridylphosphonium salts with catalytic potassium carbonate to generate pyridyl radical for pyridine synthesis.Remarkably,this light-driven transformation allowed chiral pool synthesis with excellent chirality retention,giving a wide range of chiral selenium-containing pyridines.On the basis of our combined computational and experimental studies,we propose that the PnB between pyridylphosphonium salts and potassium carbonate enables access to the photoactive charge transfer complex,which is able to undergo single electron transfer to generate pyridyl radical for its transformation.展开更多
Unsymmetric sulfides and selenides have great applications in the pharmaceutical field.Herein,we describe the reductive coupling reaction of xanthate esters with sulfur-containing and selenium-containing compounds(thi...Unsymmetric sulfides and selenides have great applications in the pharmaceutical field.Herein,we describe the reductive coupling reaction of xanthate esters with sulfur-containing and selenium-containing compounds(thio(seleno)sulfonates and disulfides(selenides))under the nickel-catalyzed condition.It provides a mild and effective method for the synthesis of unsymmetric sulfides and selenides which has the advantages of mild reaction conditions,high yields and a wide range of substrates.展开更多
Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of...Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of external signals into physiological responses.Sequential transmission of biological signals from one enzyme to the next promotes signal transduction with feedforward and feedback mechanisms.Reconstructing these processes in an artificial system provides potential applications and offers a new way to understand fundamental biological processes in depth.However,the design of artificial signal transduction systems regulated by artificial enzyme receptors in a predictable and intelligent manner remains a challenge.Herein,benefiting from the polarity-regulated characteristics of Se-containing compounds with artificial glutathione peroxidase(GPx)activity,we constructed an artificial transmembrane signaling receptor with a Se-containing GPx-like recognition head group,a membrane-anchoring group,and a pre-enzyme end group.The artificial supramolecular signal transduction system containing such signal transduction receptors extends the range of signaling systems based on enzyme regulation,which provides a new way to study natural signal processes in cells and artificially regulated biological processes.展开更多
A selenium-functionalizedε-caprolactone was synthesized by introducing a phenyl selenide group at the 7-position.A polymer was obtained through the ring-opening polymerization of this monomer in a base/thiourea binar...A selenium-functionalizedε-caprolactone was synthesized by introducing a phenyl selenide group at the 7-position.A polymer was obtained through the ring-opening polymerization of this monomer in a base/thiourea binary organocatalytic system.A living polymerization process was achieved under mild conditions.The resulting polymers had a controlled molecular weight with a narrow molecular weight distributions and high end-group fidelity.Random copolymers could be obtained by copolymerizing this monomer withε-caprolactone.The thermal degradation temperature of the obtained copolymers decreased with the increasing molar ratio of selenide functionalized monomer in copolymers,while the glass transition temperature increased.In addition,the phenyl selenide side group could be further modified to a polyselenonium salt,which resulted in a polymer with good antibacterial properties.The survival rate of E.coli and S.aureus was only 9%with a polymer concentration of 62.5μg/mL.展开更多
A novel selenium-containing compound,selenoneine,has been isolated as the major form of organic selenium in the blood and tissues of tuna.Selenoneine harbors a selenium atom in the imidazole ring,2-selenylNα,Nα,Nα-...A novel selenium-containing compound,selenoneine,has been isolated as the major form of organic selenium in the blood and tissues of tuna.Selenoneine harbors a selenium atom in the imidazole ring,2-selenylNα,Nα,Nα-trimethyl-L-histidine,and is a selenium analog of ergothioneine.This selenium compound has strong antioxidant capacity and binds to heme proteins,such as hemoglobin and myoglobin,to protect them from iron auto-oxidation,and it reacts with radicals and methylmercury(MeHg) .The organic cations/carnitine transporter OCTN1 transports selenoneine and MeHg,regulates Se-enhanced antioxidant activity,and decreases MeHg toxicity.Thus,the dietary intake of selenoneine,by consuming fish,might decrease the formation of reactive oxygen radicals that could oxidize nucleotides in DNA,and thereby inhibit carcinogenesis,chronic diseases,and aging.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.30870540,30970633)
文摘As one of the most important antioxidant enzymes, glutathione peroxidase（GPX） protects cells and tissues from oxidative damage, and plays an important role in cardiovascular and cerebrovascular injuries induced by oxida- tive stress. The antioxidant effect of selenium-containing glutathione S-transferase（Se-GST）, a mimic of GPX was investigated on rat cardiomyocytes. To explore the protection function of Se-GST in hydrogen peroxide（H202） chal- lenged rat cardiomyocytes, we examined malondialdehyde（MDA）, lactate dehydrogenase（LDH）, superoxide dismu- tase（SOD） and cell apoptosis. The results demonstrate exposure of rat cardiomyocytes to H202 for 6 and 12 h induced the significant increases of MDA, LDH and apoptosis rate of cardiomyocytes, but pretreatment of rat cardiomyocytes with Se-GST at 0.0005 or 0.001 unit/mL prevents oxidative stress induced by H202 with the decreases of cell apopto- sis. All the results hint Se-GST has antioxidant activity for oxidative stress challenged rat cardiomyocvtes.
基金supported by the Beijing Natural Science Foundation(Grant Numbers 7232221 and L222030)the National Natural Science Foundation of China(Grant Number 82271032)+1 种基金the National Key R&D Program of China(Grant Number 2023YFA0915300)the XPLORER PRIZE and the Beijing Outstanding Young Scientist Program.
文摘Diabetic wound healing impairment,a common complication of diabetes,has limited clinical treatment options and poor therapeutic outcomes,causing significant physical pain and psychological burden for patients.This study aims to accelerate wound healing by modulating cellular stress responses,offering a safe and efficient new therapeutic strategy.Herein,a seleniumcontaining polyurethane(SePU)thermo-sensitive hydrogel was synthesized,and its mechanism for promoting diabetic wound healing by activating the unfolded protein response(UPR)was elucidated.Hydroxybutyl chitosan(HBC)offers amore convenient application for SePU,with its high hydroxybutyl substitution enabling the hydrogel to undergo a rapid sol-gel transition at physiological temperatures.In vitro experiments showed that SePU thermo-sensitive hydrogel(SePU/HBC),at appropriate concentrations,significantly promoted the proliferation,spreading,migration,and adhesion of human skin fibroblasts(HSFs),while inhibiting inflammation.In vivo diabeticmouse model,SePU/HBC exhibited a significant wound-healing effect,promoting re-epithelialization,collagen formation and maturation.Mechanistic studies revealed that SePU/HBC alleviated endoplasmic reticulum stress under hyperglycemic conditions by activating the UPR-related gene ATF6 to alleviate endoplasmic reticulum stress(ERS)and inhibit apoptosis.This study offers a novel strategy for diabetic wound treatment using SePU/HBC,which activates the UPR and inhibits apoptosis,demonstrating promising clinical applications for wound healing.
基金supported by grants from the National Natural Science Foundation of China(22101279,22001248)Fundamental Research Funds for the Central Universities,the University of the Chinese Academy of Sciences and Binzhou Institute of Technology,Weiqiao-UCAS Science and Technology Park.
文摘Pyridylphosphonium salts,which are readily available and air and thermally stable,have been used to effectively synthesize structurally diverse pyridines.Herein,we report the pnictogen bonding(PnB)enabled photoactivation of pyridylphosphonium salts with catalytic potassium carbonate to generate pyridyl radical for pyridine synthesis.Remarkably,this light-driven transformation allowed chiral pool synthesis with excellent chirality retention,giving a wide range of chiral selenium-containing pyridines.On the basis of our combined computational and experimental studies,we propose that the PnB between pyridylphosphonium salts and potassium carbonate enables access to the photoactive charge transfer complex,which is able to undergo single electron transfer to generate pyridyl radical for its transformation.
基金National Natural Science Foundation of China(Nos.21971174 and U2267224),PAPD,Cyrus Tang(Zhongying)Scholar,Jiangsu Qing-Lan Project for Young and Middle-aged Academic Leaders(2023)and Soochow University for financialsupport.
文摘Unsymmetric sulfides and selenides have great applications in the pharmaceutical field.Herein,we describe the reductive coupling reaction of xanthate esters with sulfur-containing and selenium-containing compounds(thio(seleno)sulfonates and disulfides(selenides))under the nickel-catalyzed condition.It provides a mild and effective method for the synthesis of unsymmetric sulfides and selenides which has the advantages of mild reaction conditions,high yields and a wide range of substrates.
基金This work was supported by the National Key Research and Development Program of China(Nos.2020YFA0908500 and 2018YFA0901600)the National Natural Science Foundation of China(No.22161142015).
文摘Signal transduction across lipid bilayers is of profound importance in biological processes.In biological systems,natural enzymes mediate biochemical effects by binding to substrates and facilitating the conversion of external signals into physiological responses.Sequential transmission of biological signals from one enzyme to the next promotes signal transduction with feedforward and feedback mechanisms.Reconstructing these processes in an artificial system provides potential applications and offers a new way to understand fundamental biological processes in depth.However,the design of artificial signal transduction systems regulated by artificial enzyme receptors in a predictable and intelligent manner remains a challenge.Herein,benefiting from the polarity-regulated characteristics of Se-containing compounds with artificial glutathione peroxidase(GPx)activity,we constructed an artificial transmembrane signaling receptor with a Se-containing GPx-like recognition head group,a membrane-anchoring group,and a pre-enzyme end group.The artificial supramolecular signal transduction system containing such signal transduction receptors extends the range of signaling systems based on enzyme regulation,which provides a new way to study natural signal processes in cells and artificially regulated biological processes.
基金financially supported by the National Natural Science Foundation of China(No.21871200)the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions。
文摘A selenium-functionalizedε-caprolactone was synthesized by introducing a phenyl selenide group at the 7-position.A polymer was obtained through the ring-opening polymerization of this monomer in a base/thiourea binary organocatalytic system.A living polymerization process was achieved under mild conditions.The resulting polymers had a controlled molecular weight with a narrow molecular weight distributions and high end-group fidelity.Random copolymers could be obtained by copolymerizing this monomer withε-caprolactone.The thermal degradation temperature of the obtained copolymers decreased with the increasing molar ratio of selenide functionalized monomer in copolymers,while the glass transition temperature increased.In addition,the phenyl selenide side group could be further modified to a polyselenonium salt,which resulted in a polymer with good antibacterial properties.The survival rate of E.coli and S.aureus was only 9%with a polymer concentration of 62.5μg/mL.
基金Supported by In part grants from the Ministry of Agriculture,Forestry,and Fisheries of Japan(Rural Biomass Research Project,BM-D2300)and Fisheries Research Agency
文摘A novel selenium-containing compound,selenoneine,has been isolated as the major form of organic selenium in the blood and tissues of tuna.Selenoneine harbors a selenium atom in the imidazole ring,2-selenylNα,Nα,Nα-trimethyl-L-histidine,and is a selenium analog of ergothioneine.This selenium compound has strong antioxidant capacity and binds to heme proteins,such as hemoglobin and myoglobin,to protect them from iron auto-oxidation,and it reacts with radicals and methylmercury(MeHg) .The organic cations/carnitine transporter OCTN1 transports selenoneine and MeHg,regulates Se-enhanced antioxidant activity,and decreases MeHg toxicity.Thus,the dietary intake of selenoneine,by consuming fish,might decrease the formation of reactive oxygen radicals that could oxidize nucleotides in DNA,and thereby inhibit carcinogenesis,chronic diseases,and aging.
