One-day-old healthy ducklings （n = 100） were randomly allocated into two groups （n = 50, respectively） to investigate the effect of cytokines on ducklings with selenium poisoning. The ducklings in control group （...One-day-old healthy ducklings （n = 100） were randomly allocated into two groups （n = 50, respectively） to investigate the effect of cytokines on ducklings with selenium poisoning. The ducklings in control group （Group Ⅰ） were fed with full rations containing 0.3 mg kg^-1 selenium, and the ducklings in selenium poisoning group （Group Ⅱ ） were fed with seleniumexcessive rations containing 8.0 mg kg^-1 selenium. One mL blood was collected by cardiac puncture at d 15, 30, 60, and 90 （n = 10, respectively）, serum was separated by centrifugation. Contents of cytokines were determined by the radioimmunity method （RIA）. The results showed that the contents of IL-113, IL-213 in Group Ⅱ were higher than that in control group at d 15 （P〉0.05）, but were significantly lower at d 30, 60, and 90 （P〈0.01）; the contents of IL-8β in Group II were significantly lower than that in control group （P〈0.01）; the contents of erythropoietin （EPO） and IGF-2 in Group II were significantly lower in different degree than that in control group at d 60 and 90 （P〈0.01）; the contents of granulocyte- macrophage colony stimulating factor （GM-CSF） in the two groups were lower in different degree and significantly lower at d 60 and 90 （P〈0.01）. It can be concluded that the excessive selenium could result in a decrease in immunity and a series of clinical pathology changes, such as anemia, tissue hemorrhage, and necrosis.展开更多
One-day-old healthy ducklings(n=100)were divided into control group and experimental group randomly, and selenium poisoning of ducklings was artificially caused by feeding ration containing 8 mg kg-1 Se every day. The...One-day-old healthy ducklings(n=100)were divided into control group and experimental group randomly, and selenium poisoning of ducklings was artificially caused by feeding ration containing 8 mg kg-1 Se every day. The dynamic changes of No content and NOS activity in the serum and tissues were determined by means of the method of nitric acid reductase. Results showed that the NO content and NOS activity in serum and tissues in experimental group increased significantly(P<0. 05)and they were time-dependent. It was suggested that the high level of selenium in bodies could increase the NOS activity and NO content as it destroyed the metabolism of material and energy as well as structure and function of tissues and cells. These changes of NO content and NOS activity might be involved in the metabolism of selenium poisoning.展开更多
[ Objective] This study was to investigate the effect of selenium（Se） on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divid...[ Objective] This study was to investigate the effect of selenium（Se） on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divided into three groups, two testing groups and one control group. For each group, Se content in whole blood, immune function of erythrocytes, activity of whole blood glutathione peroxidase（GSH-Px）, activity of blood plasma superoxide dismutase（SOD）, and blood plasma malondialdehyde（MDA） content were determined. [ Result] Compared with the control group, Se content in whole blood and blood plasma MDA content increased remarkably, while whole blood GSH-Px activity and blood plasma SOD activity decreased; RBC-C1 RR assumed a rise-fall trend, and RBC-ICR showed no obvious change. [Condusion] Se poisoning can reduce the activity of antioxidant enzymes, disturb the balance of oxygen free radicals metabolism, thereby inducing erythrooyte immune function in piglets.展开更多
In order to to provide scientific reference for the management and development of selenium resources in Enshi,four soil samples and four relevant rice samples were collected from four regions of Enshi,and then seleniu...In order to to provide scientific reference for the management and development of selenium resources in Enshi,four soil samples and four relevant rice samples were collected from four regions of Enshi,and then selenium content in the soil and rice samples were measured by ICP-MS. Afterwards,the occurrence forms of selenium in the soil samples were detected by continuous extraction. At last,the bioavailability of selenium in rice was studied by the in vitro gastrointestinal model. The results showed that selenium content in soil was 0. 15-5. 42 mg/kg in Enshi. The proportion of water-soluble selenium in soil was the lowest,ranging from 1. 41% to 3. 80%; the proportion of residual selenium was the highest,reaching 36. 1%-41. 7%. Besides,selenium content in rice was 0. 07-1. 61 mg/kg,and the bioconcentration factor of selenium in rice was 0. 22-0. 48. The bioaccessibility of selenium in rice ranged from 45. 7% to 56. 4% in the stomach and from 58. 4% to68. 5% in the small intestine. In addition,the daily intake of selenium per capita( PDI) in Yutangba,Changping,Shadi,and Taiyanghe was490,26,132 and 57 μg/d,indicating that people in Yutangba had the risk of chronic selenium poisoning,and people's diet was rich in selenium in Shadi and Taiyanghe,while people's diet was deficient in selenium in Changping.展开更多
文摘One-day-old healthy ducklings （n = 100） were randomly allocated into two groups （n = 50, respectively） to investigate the effect of cytokines on ducklings with selenium poisoning. The ducklings in control group （Group Ⅰ） were fed with full rations containing 0.3 mg kg^-1 selenium, and the ducklings in selenium poisoning group （Group Ⅱ ） were fed with seleniumexcessive rations containing 8.0 mg kg^-1 selenium. One mL blood was collected by cardiac puncture at d 15, 30, 60, and 90 （n = 10, respectively）, serum was separated by centrifugation. Contents of cytokines were determined by the radioimmunity method （RIA）. The results showed that the contents of IL-113, IL-213 in Group Ⅱ were higher than that in control group at d 15 （P〉0.05）, but were significantly lower at d 30, 60, and 90 （P〈0.01）; the contents of IL-8β in Group II were significantly lower than that in control group （P〈0.01）; the contents of erythropoietin （EPO） and IGF-2 in Group II were significantly lower in different degree than that in control group at d 60 and 90 （P〈0.01）; the contents of granulocyte- macrophage colony stimulating factor （GM-CSF） in the two groups were lower in different degree and significantly lower at d 60 and 90 （P〈0.01）. It can be concluded that the excessive selenium could result in a decrease in immunity and a series of clinical pathology changes, such as anemia, tissue hemorrhage, and necrosis.
文摘One-day-old healthy ducklings(n=100)were divided into control group and experimental group randomly, and selenium poisoning of ducklings was artificially caused by feeding ration containing 8 mg kg-1 Se every day. The dynamic changes of No content and NOS activity in the serum and tissues were determined by means of the method of nitric acid reductase. Results showed that the NO content and NOS activity in serum and tissues in experimental group increased significantly(P<0. 05)and they were time-dependent. It was suggested that the high level of selenium in bodies could increase the NOS activity and NO content as it destroyed the metabolism of material and energy as well as structure and function of tissues and cells. These changes of NO content and NOS activity might be involved in the metabolism of selenium poisoning.
文摘[ Objective] This study was to investigate the effect of selenium（Se） on immune function and oxygen free radicals in erythrocyte of pig. [ Method] Fifteen weanling Landrace piglets as experimental animals were divided into three groups, two testing groups and one control group. For each group, Se content in whole blood, immune function of erythrocytes, activity of whole blood glutathione peroxidase（GSH-Px）, activity of blood plasma superoxide dismutase（SOD）, and blood plasma malondialdehyde（MDA） content were determined. [ Result] Compared with the control group, Se content in whole blood and blood plasma MDA content increased remarkably, while whole blood GSH-Px activity and blood plasma SOD activity decreased; RBC-C1 RR assumed a rise-fall trend, and RBC-ICR showed no obvious change. [Condusion] Se poisoning can reduce the activity of antioxidant enzymes, disturb the balance of oxygen free radicals metabolism, thereby inducing erythrooyte immune function in piglets.
基金Supported by National High Technology Research and Development Program of China(863 Program)(2012AA06A204)
文摘In order to to provide scientific reference for the management and development of selenium resources in Enshi,four soil samples and four relevant rice samples were collected from four regions of Enshi,and then selenium content in the soil and rice samples were measured by ICP-MS. Afterwards,the occurrence forms of selenium in the soil samples were detected by continuous extraction. At last,the bioavailability of selenium in rice was studied by the in vitro gastrointestinal model. The results showed that selenium content in soil was 0. 15-5. 42 mg/kg in Enshi. The proportion of water-soluble selenium in soil was the lowest,ranging from 1. 41% to 3. 80%; the proportion of residual selenium was the highest,reaching 36. 1%-41. 7%. Besides,selenium content in rice was 0. 07-1. 61 mg/kg,and the bioconcentration factor of selenium in rice was 0. 22-0. 48. The bioaccessibility of selenium in rice ranged from 45. 7% to 56. 4% in the stomach and from 58. 4% to68. 5% in the small intestine. In addition,the daily intake of selenium per capita( PDI) in Yutangba,Changping,Shadi,and Taiyanghe was490,26,132 and 57 μg/d,indicating that people in Yutangba had the risk of chronic selenium poisoning,and people's diet was rich in selenium in Shadi and Taiyanghe,while people's diet was deficient in selenium in Changping.
