Hyperoside is one of the major components of Hypericum perforatum L. and also present in many plant species such as Abelmoschus manihot （L.） Medik., Ribes nigrum L. and Rosa agrestis Savi （Rosaceae）. Because hyper...Hyperoside is one of the major components of Hypericum perforatum L. and also present in many plant species such as Abelmoschus manihot （L.） Medik., Ribes nigrum L. and Rosa agrestis Savi （Rosaceae）. Because hyperoside exhibits many biological activities, the pharmacokinetics profile of hyperoside needs to be studied for further elucidating its mechanism of action. A simple method for the determination of hyperoside in rat plasma was developed by using ultra-high performance liquid chromatography coupled with mass spectrometry （UPLC-MS）. Only 50 ~tL plasma samples were required for sample preparation. The quantitative detection of hyperoside was accomplished by selected ion monitoring （SIM） in negative ion mode. Hyperoside was analyzed in less than 10 rain. Good linearity was obtained （r2〉0.999） and the intra- and inter-day precision of the method were lower than 15%. Lower limit of quantification （LLOQ） was 4 ng/mL for hyperoside in rat plasma. Our method showed advantage in the lower LLOQ compared with the reported method; furthermore, smaller amount of plasma was needed. The method was successfully applied for the pharmacokinetics study of hyperoside in rat after intravenous administration of hyperoside.展开更多
A novel preparation method of phosphorus pentafluoride（PF5） is introduced in details in the article. Phosphorus pentafluoride is mainly used to synthesize lithium ion battery electrolyte salt. In the experiment mesn...A novel preparation method of phosphorus pentafluoride（PF5） is introduced in details in the article. Phosphorus pentafluoride is mainly used to synthesize lithium ion battery electrolyte salt. In the experiment mesneproduct- hexafluorophosphoric acid（HPF6） was firstly prepared-from phosphorus pentoxide（P2O5） and anhydrous hydrogen fluoride（HF）, according to the chemical stoichiometrics, the optimum amount of excess HF used was about 60% and the optimum reaction time was 4 h; then HPF6 was dehydrated by oleum with an excess amount of 35%-- 40% which was heated for 40--50 min at 150 ℃; thus HPF6 was decomposed to a mixture of gases containing PF5; impurity gases were removed through condensation in the presence of cooling water at 0 ℃, then PF5 of high purity was eventually prepared. The production was characterized by inffared（IR） spectrometry and gas chromatography-mass spectroscopy-selected ion monitoring（GC-MS-SIM）. Eventual results indicate that PF5 prepared by the novel method has a high target purity and a low amount of impurity and water.展开更多
Volatilomics is essential for understanding the biological functions and fragrance contributions of plant volatiles.However,the annotation coverage achieved using current untargeted and widely targeted volatomics(WTV)...Volatilomics is essential for understanding the biological functions and fragrance contributions of plant volatiles.However,the annotation coverage achieved using current untargeted and widely targeted volatomics(WTV)methods has been limited by low sensitivity and/or low acquisition coverage.Here,we introduce WTV 2.0,which enabled the construction of a high-coverage library containing 2111 plant volatiles,and report the development of a comprehensive selective ion monitoring(cSIM)acquisition method,including the selection of characteristic qualitative ions with the minimal ion number for each compound and an optimized segmentation method,that can acquire the smallest but sufficient number of ions for most plant volatiles,as well as the automatic qualitative and semi-quantitative analysis of cSIM data.Importantly,the library and acquisition method we developed can be self-expanded by incorporating compounds not present in the library,utilizing the obtained cSIM data.We showed that WTV 2.0 increases the median signal-to-noise ratio by 7.6-fold compared with the untargeted method,doubled the annotation coverage compared with the untargeted and WTV 1.0 methods in tomato fruit,and led to the discovery of menthofuran as a novel flavor compound in passion fruit.WTV 2.0 is a Python library with a user-friendly interface and is applicable to profiling of volatiles and primary metabolites in any species.展开更多
A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in toba...A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in tobacco samples were extracted by methylene chloride using simultaneous distillation and extraction equipment,and they were analyzed by HP-INNOWAX column(30m×250μm×0.25μm).Thirteen acidic components were quantitatively determined by internal standard curve method.The experiment results showed that the added standard recoveries of the acidic components were in the range from 80.6% to 98.8%,the relative standard deviations(RSD)were less than 2.0% and the correlation coefficients were more than 0.99.The method is simple,rapid and accurate for the determination of the volatile and semi-volatile acidic components in tobacco.展开更多
文摘Hyperoside is one of the major components of Hypericum perforatum L. and also present in many plant species such as Abelmoschus manihot （L.） Medik., Ribes nigrum L. and Rosa agrestis Savi （Rosaceae）. Because hyperoside exhibits many biological activities, the pharmacokinetics profile of hyperoside needs to be studied for further elucidating its mechanism of action. A simple method for the determination of hyperoside in rat plasma was developed by using ultra-high performance liquid chromatography coupled with mass spectrometry （UPLC-MS）. Only 50 ~tL plasma samples were required for sample preparation. The quantitative detection of hyperoside was accomplished by selected ion monitoring （SIM） in negative ion mode. Hyperoside was analyzed in less than 10 rain. Good linearity was obtained （r2〉0.999） and the intra- and inter-day precision of the method were lower than 15%. Lower limit of quantification （LLOQ） was 4 ng/mL for hyperoside in rat plasma. Our method showed advantage in the lower LLOQ compared with the reported method; furthermore, smaller amount of plasma was needed. The method was successfully applied for the pharmacokinetics study of hyperoside in rat after intravenous administration of hyperoside.
基金Supported by the National Basic Research Program of China(No.2007CB613607)
文摘A novel preparation method of phosphorus pentafluoride（PF5） is introduced in details in the article. Phosphorus pentafluoride is mainly used to synthesize lithium ion battery electrolyte salt. In the experiment mesneproduct- hexafluorophosphoric acid（HPF6） was firstly prepared-from phosphorus pentoxide（P2O5） and anhydrous hydrogen fluoride（HF）, according to the chemical stoichiometrics, the optimum amount of excess HF used was about 60% and the optimum reaction time was 4 h; then HPF6 was dehydrated by oleum with an excess amount of 35%-- 40% which was heated for 40--50 min at 150 ℃; thus HPF6 was decomposed to a mixture of gases containing PF5; impurity gases were removed through condensation in the presence of cooling water at 0 ℃, then PF5 of high purity was eventually prepared. The production was characterized by inffared（IR） spectrometry and gas chromatography-mass spectroscopy-selected ion monitoring（GC-MS-SIM）. Eventual results indicate that PF5 prepared by the novel method has a high target purity and a low amount of impurity and water.
基金supported by key project of regional joint fund of National Natural Science FoundationNational Natural Science Foundation of China(U22A20476)Hainan international science and technology cooperation research and development project(GHYF2023005)+3 种基金Sanya Yazhou Sci-Tech City(SYND-2022-02).)Hainan Yazhou Bay Seed Lab(Nono.B21HJ0903)“111”Project111 Project(Nono.D20024).)Hainan Provincial Natural Science Foundation of China Hainan Provincial Natural Science Foundation of China(320MS011).)‘PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City(HSPHDSRF-2023-12-001).)’Basic Research Project in 2023 of Yazhouwan National Laboratory.
文摘Volatilomics is essential for understanding the biological functions and fragrance contributions of plant volatiles.However,the annotation coverage achieved using current untargeted and widely targeted volatomics(WTV)methods has been limited by low sensitivity and/or low acquisition coverage.Here,we introduce WTV 2.0,which enabled the construction of a high-coverage library containing 2111 plant volatiles,and report the development of a comprehensive selective ion monitoring(cSIM)acquisition method,including the selection of characteristic qualitative ions with the minimal ion number for each compound and an optimized segmentation method,that can acquire the smallest but sufficient number of ions for most plant volatiles,as well as the automatic qualitative and semi-quantitative analysis of cSIM data.Importantly,the library and acquisition method we developed can be self-expanded by incorporating compounds not present in the library,utilizing the obtained cSIM data.We showed that WTV 2.0 increases the median signal-to-noise ratio by 7.6-fold compared with the untargeted method,doubled the annotation coverage compared with the untargeted and WTV 1.0 methods in tomato fruit,and led to the discovery of menthofuran as a novel flavor compound in passion fruit.WTV 2.0 is a Python library with a user-friendly interface and is applicable to profiling of volatiles and primary metabolites in any species.
文摘A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in tobacco samples were extracted by methylene chloride using simultaneous distillation and extraction equipment,and they were analyzed by HP-INNOWAX column(30m×250μm×0.25μm).Thirteen acidic components were quantitatively determined by internal standard curve method.The experiment results showed that the added standard recoveries of the acidic components were in the range from 80.6% to 98.8%,the relative standard deviations(RSD)were less than 2.0% and the correlation coefficients were more than 0.99.The method is simple,rapid and accurate for the determination of the volatile and semi-volatile acidic components in tobacco.
