探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习...探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习行为,推动全员参与、全过程管理和全方位覆盖的学风建设模式,以提升护理本科生的学习投入度和信息素养为主要目标,为护理教育提供新的实践路径。展开更多
Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practica...Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.展开更多
Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used...Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used for producing activated charcoal and in environmental remediation,their pharmacological potential remains underexplored.This study aimed to evaluate the chemical composition,biological activity,and potential commercial value of seeds from four widely consumed Saharan cultivars(Assiane,Boufegouss,Aziza,and Majhoul)collected in the Figuig region.Soxhlet extraction was employed to obtain seed extracts,which were subsequently characterized by qualitative and quantitative phytochemical screening.The analyses revealed the presence of polyphenols,flavonoids,terpenoids,steroids,alkaloids,and other secondary metabolites.The Aziza and Boufegouss varieties were particularly rich in flavonoids(82.09-139.61 mg quercetin equivalents/100 g dry matter)and total polyphenols(69.81-1183.96 mg gallic acid equivalents/100 g dry matter).Mineral profiling confirmed the occurrence of essential trace elements,including Zn,Cu,Fe,and Co.To investigate their antidiabetic potential,in vitro assays ofα-glucosidase andα-amylase inhibition were performed.Acetone extracts demonstrated significant inhibitory activity against both enzymes,suggesting a promising role of P.dactylifera seed extracts as natural agents for the management of type 2 diabetes mellitus.展开更多
Thousand-seed weight(TSW)is a critical target for genetic improvement in rapeseed(Brassica napus L.).However,phenotypic selection for this trait remains challenging due to its polygenic regulation by multiple quantita...Thousand-seed weight(TSW)is a critical target for genetic improvement in rapeseed(Brassica napus L.).However,phenotypic selection for this trait remains challenging due to its polygenic regulation by multiple quantitative trait loci(QTL).Here,six favorable TSW QTL alleles from two donor parents were introgress into an elite restorer line,621R,using an integrated strategy combining marker-assisted backcrossing and speed breeding protocols.Through six rounds of backcrossing and convergent crossing followed by two generations of selfing strategies,we developed 13 advanced lines with diverse TSW QTL combinations within 24 months.Field evaluations across three environments revealed that all lines exhibited significantly increased TSW in spring conditions(Minle,Gansu)and winter environments(Wuhan and Jiangling,Hubei)except for two lines which only showed increase in the spring environment.Hybridization assays using these lines as male parents crossed with two male-sterile lines(RG430A and 616A)demonstrated transgressive segregation for TSW:For RG430A-derived hybrids,all crosses significantly outperformed the original control(RG430A×621R)in Wuhan,with 8/13 and 9/13 crosses showing significant TSW increases in Minle and Jiangling,respectively.For 616A-derived hybrids,11/13 and 10/13 crosses exhibited significant TSW enhancement in Minle and Jiangling,compared to 3/13 in Wuhan.Notably,two top-performing hybrids achieved 13.0%and 6.8%higher plot yields,respectively.Our results demonstrate that strategic pyramiding of complementary TSW QTL alleles effectively enhances seed weight in rapeseed,and these improved lines represent valuable genetic resources for developing high-yield hybrids.展开更多
Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).Ho...Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).展开更多
Neoadjuvant therapy(NAT)has become the standard treatment for patients with locally advanced breast cancer and stage II-III HER2-positive(HER2+)or triple-negative breast cancer(TNBC)1,2.It is essential to accurately m...Neoadjuvant therapy(NAT)has become the standard treatment for patients with locally advanced breast cancer and stage II-III HER2-positive(HER2+)or triple-negative breast cancer(TNBC)1,2.It is essential to accurately mark the primary breast tumor and positive axillary lymph nodes(ALNs)prior to NAT to ensure precise surgical excision,guide axillary downstaging,and guarantee reliable lesion retrieval for pathologic evaluation3.The false-negative rate of sentinel lymph node biopsy(SLNB)after NAT can be reduced to<10%by applying modalities,such as the identification of≥3 sentinel lymph nodes(SLNs)with dual-mapping techniques or removal of the marked lymph node with target axillary dissection(TAD)according to the ASCO,NCCN,and CBCS guidelines3-5.However,there is a lack of consensus regarding the optimal methods and materials for accurate marking6,7.Conventional techniques include clip placement,guidewire localization,and carbon or ink tattooing,whereas wireless technologies,such as MagseedR,radiofrequency identification tags,SAVI SCOUTR,and radioactive iodine-125(125I)seeds,have also been adopted.Traditional marking techniques have a localization failure rate of approximately 10%.In contrast,the use of 125I seeds(with a radiation dose of 0.1-0.3 mCi)has significantly improved localization accuracy8,9.Nevertheless,owing to radioactive properties,concerns have been raised regarding the potential impact of 125I seed marking on assessing the pathologic complete response(pCR)after NAT10.Moreover,whether the influence of 125I seed marking on pCR could lead to suboptimal adjuvant treatment decisions and potentially compromise long-term oncologic outcomes has not been established.To investigate the potential impact of 125I seed placement on the pCR rate and long-term outcomes in breast cancer patients receiving NAT,we conducted a retrospective cohort study utilizing propensity score matching(PSM).展开更多
Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitig...Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitigates oxidative stress-induced cellular senescence and dysfunction in melanocytes.In accordance with established protocols,the ethanol fraction from Nigella sativa L.seeds was extracted,concentrated,and lyophilized to evaluate its herbal effects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays,tyrosinase activity evaluation,measurement of cellular melanin contents,scratch assays,senescence-associatedβ-galactosidase(SA-β-gal)staining,enzyme-linked immunosorbent assay(ELISA),and Western blot analysis for expression profiling of experimentally relevant proteins.The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr,Mitf,and gp100 in B16F10 cells.Furthermore,HZC effectively mitigated oxidative stress-induced cellular senescence,improved melanocyte condition,and rectified various functional impairments associated with melanocyte dysfunction.These findings suggest that HZC increases melanin synthesis in melanocytes through the activation of the MAPK,PKA,and Wnt signaling pathways.In addition,HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuclear factor E2-related factor 2-antioxidant response element(Nrf2-ARE)pathway and enhancing the activity of downstream antioxidant enzymes,thus preventing premature senescence and dysfunction in melanocytes.展开更多
Rapid,accurate seed classification of soybean varieties is needed for product quality control.We describe a hyperspectral image-based deep-learning model called Dual Attention Feature Fusion Networks(DAFFnet),which se...Rapid,accurate seed classification of soybean varieties is needed for product quality control.We describe a hyperspectral image-based deep-learning model called Dual Attention Feature Fusion Networks(DAFFnet),which sequentially applies 3D Convolutional Neural Network(CNN)and 2D CNN.A fusion attention mechanism module in 2D CNN permits the model to capture local and global feature information by combining with Convolution Block Attention Module(CBAM)and Mobile Vision Transformer(MViT),outperforming conventional hyperspectral image classification models in seed classification.展开更多
Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel ...Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel chemotherapy drugs that combine traditional Chinese medicine(TCM)with herbal extracts exhibit superior comprehensive benefits.Herein,we delved into an article authored by Wang et al,focusing specifically on the pharmacological effects of"Herba Patriniae and Coix seed(HC)"and their targeted mechanisms in combating CRC.From the perspective of TCM philosophy,damp-heat stagnation and toxicity are the cardinal pathogenic factors underlying CRC.HC,renowned for their abilities to antipyretic and enhance diuresis,have demonstrated promising efficacy in preliminary studies for the treatment of CRC.These findings offer potential insights in favor of fostering anti-cancer medications.展开更多
The cleanliness of seed cotton plays a critical role in the pre-treatment of cotton textiles,and the removal of impurity during the harvesting process directly determines the quality and market value of cotton textile...The cleanliness of seed cotton plays a critical role in the pre-treatment of cotton textiles,and the removal of impurity during the harvesting process directly determines the quality and market value of cotton textiles.By fusing band combination optimization with deep learning,this study aims to achieve more efficient and accurate detection of film impurities in seed cotton on the production line.By applying hyperspectral imaging and a one-dimensional deep learning algorithm,we detect and classify impurities in seed cotton after harvest.The main categories detected include pure cotton,conveyor belt,film covering seed cotton,and film adhered to the conveyor belt.The proposed method achieves an impurity detection rate of 99.698%.To further ensure the feasibility and practical application potential of this strategy,we compare our results against existing mainstream methods.In addition,the model shows excellent recognition performance on pseudo-color images of real samples.With a processing time of 11.764μs per pixel from experimental data,it shows a much improved speed requirement while maintaining the accuracy of real production lines.This strategy provides an accurate and efficient method for removing impurities during cotton processing.展开更多
Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet ful...Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet fully understood.Therefore,determining the genetic mechanisms and key regulatory genes of seedless grapes is of great significance for seedless grape breeding and meeting market demands.The emergence of high-throughput analysis software offers greater possibilities for mining genes related to plant organ development.Specifically,to mine a greater number of candidate genes related to grape seed traits,this study used the seed trait parameters analyzed by Tomato Analyzer as the target trait and then used a genome-wide association study(GWAS)to mine candidate genes.In the two-year analysis using principal component analysis(PCA),we extracted five principal components with a cumulative contribution rate of 96.586%.The cumulative contribution rate for component 1 reached 87.352%.Correlation analysis revealed correlation coefficients ranging from 0.54 to 0.98 among the seven basic traits.The GWAS results indicated that 370 SNP loci were significantly correlated with seed traits.These SNP loci were distributed on 18 chromosomes,except for chromosome 4,with most SNP loci distributed on chromosome 18.Based on the physical location of single nucleotide polymorphism(SNP)markers significantly associated with seed-related traits in the grape reference genome,candidate genes are screened within the range of linkage disequilibrium(LD)attenuation distance,both upstream and downstream of the significant SNP loci.These candidate genes were mainly transcription factor-related genes(VvMADS4 and VvMADS5),ubiquitin ligase-related genes(E3 ubiquitin ligase BIG BROTHER),serine/threonine protein kinase-related genes,and carbohydrate metabolism-related genes(Sucrose Synthase 2)and simultaneously controlled multiple(at least two or more)seed traits.These results indicate that seed traits are jointly regulated by some genes involved in seed morphology regulation.In this work,we identified new gene loci related to grape seed traits.Identifying molecular markers closely related to these seed traits is of great significance for breeding seedless grape varieties.展开更多
Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their po...Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their population dynamics may directly influence the future alpine ecosystems.However,little is known about how climate warming affect cushion population recruitment,especially at early life-history stages.In this study,we conducted a laboratory simulation of climate warming to detect the effects of warming temperature and associated moisture and light changes on seed germination and seedling growth of the typical alpine cushion plant Arenaria oreophila.Results suggested that increasing temperature indeed exerted strong constraints on the population recruitment processes.Specifically,increased temperatures could quickly initialize seed germination(4e6 days at higher temperatures vs.29 e32 days at low temperature,respectively,after sowing),accelerate them to reach the maximum germination percentage(9e19 days at higher temperatures vs.57e86 days at low temperature,respectively,after sowing)and significantly accelerate seedling growth rate.However,higher temperatures accelerated seedling mortality(more than 80%).In addition,lower light availability also increased seedling mortality though it could generally increase the final seed germination percentage.The effects of water might be dependent on temperature and light.All results suggested that cushion A.oreophila is quite sensitive to climate warming which strongly constrains its seedling establishment process.We,therefore,speculate that the continuing climate warming in future will exert uncertain risks in the persistence of cushion A.oreophila,possibly by constraining the process of seedling recruitments.展开更多
The high incidence of postpartum hypogalactia hinders the healthy development of postpartum women and the next generation.Lacquer seed oil(LSO),extracted from the seeds of the lacquer tree,has been traditionally used ...The high incidence of postpartum hypogalactia hinders the healthy development of postpartum women and the next generation.Lacquer seed oil(LSO),extracted from the seeds of the lacquer tree,has been traditionally used as a dietary supplement for promoting postpartum lactation and recovery in some districts of China.However,its physiological effects have not been verified,and the mechanism and active components of LSO have not been analyzed.Thus,we applied LSO to bromocriptine-induced postpartum hypogalactia rats.The results showed that LSO supplement effectively improves bromocriptine-induced postpartum hypogalactia.LSO also increased prolactin levels reduced by bromocriptine,promoted JAK2/STAT5 and PI3K/AKT pathways and several gene expression levels of milk synthesis in mammary gland.Moreover,metabolomic and network pharmacological analysis further revealed that JAK2/STAT5,PI3K/AKT,and estrogen signaling pathway are the potential main regulatory sites for the beneficial effects of LSO on postpartum hypogalactia,and that quercetin,kaempferol,arachidonic acid,epicatechin,and β-sitosterol are the top 5 main active ingredients of LSO.Our results suggested that LSO has great potential in the application of the improvement of postpartum hypogalactia.展开更多
Torreya grandis'Merrillii'is a well-known nut in South China with high nutritional value.Severe premature seed abscission limits the industrial development of T.grandis by causing serious economic losses.Howev...Torreya grandis'Merrillii'is a well-known nut in South China with high nutritional value.Severe premature seed abscission limits the industrial development of T.grandis by causing serious economic losses.However,the physiological mechanisms of seed abscission in T.grandis remain poorly understood.To gain insight into the relationships between carbohydrate status and seed abscission,three-year-old seed-bearing branches were taken as representative materials for the entire tree.Furthermore,the time course of changes in the photosynthetic rate and the non-structural carbohydrate(NSC)dynamics were monitored in the main sources(the one-year-old and two-year-old shoots),and the dry weight and NSC levels of sinks(the seeds,current female cone cluster,and current vegetative cluster)across all seed development stages were recorded.The cumulative seed abscission rate significantly increased,reaching 91.5%from 0 to 72 days after seed protrusion time(SPT).NSC levels in the main sources significantly decreased by 56%-79%,accompanied by a significantly increased photosynthesis rate of 17.1%-49.1%during that period and increased NSC levels in the three sinks.The gene expression level of cell wall invertase(TgCWIN)was significantly correlated with sucrose,fructose,and glucose levels.The carbon storage capacity of the main sources significantly decreased from 6.03 to3.14 mmol C·d^(-1),with a stable photosynthetic capacity,from 0 to 72 days after SPT,whereas the carbon demand of the three sinks showed a continuously increasing trend from 3.14 to 7.71 mmol C·d^(-1).In addition,sucrose supplementation significantly decreased the cumulative seed abscission rate.These results suggest that storage carbohydrates play a major role in the regulatory mechanism of seed abscission in T.grandis.Our study provides a theoretical basis for improving T.grandis yield through establishing a better carbon balance between sources and sinks using timely fertilization or proper pruning procedures.展开更多
Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intro...Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intron splicing and its role in maize kernel development.PPR21 is a typical P-type PPR protein targeted to mitochondria.The ppr21 mutants are arrested in embryogenesis and endosperm development,leading to embryo lethality.Null mutations of PPR21 reduce the splicing efficiency of nad2 intron 1,2,and 4 and impair the assembly and activity of mitochondrial complex I.Previous studies show that the P-type PPR protein EMP12 is required for the splicing of identical introns.However,our protein interaction analyses reveal that PPR21 does not interact with EMP12.Instead,both PPR21 and EMP12 interact with the small MutS-related(SMR)domain-containing PPR protein 1(PPR-SMR1)and the short P-type PPR protein 2(SPR2).PPR-SMR1 interacts with SPR2,and both proteins are required for the splicing of many introns in mitochondria,including nad2 intron 1,2,and 4.These results suggest that a PPR21-(PPR-SMR1/SPR2)-EMP12 complex is involved in the splicing of nad2 introns in maize mitochondria.展开更多
In this study,eight different varieties of maize seeds were used as the research objects.Conduct 81 types of combined preprocessing on the original spectra.Through comparison,Savitzky-Golay(SG)-multivariate scattering...In this study,eight different varieties of maize seeds were used as the research objects.Conduct 81 types of combined preprocessing on the original spectra.Through comparison,Savitzky-Golay(SG)-multivariate scattering correction(MSC)-maximum-minimum normalization(MN)was identified as the optimal preprocessing technique.The competitive adaptive reweighted sampling(CARS),successive projections algorithm(SPA),and their combined methods were employed to extract feature wavelengths.Classification models based on back propagation(BP),support vector machine(SVM),random forest(RF),and partial least squares(PLS)were established using full-band data and feature wavelengths.Among all models,the(CARS-SPA)-BP model achieved the highest accuracy rate of 98.44%.This study offers novel insights and methodologies for the rapid and accurate identification of corn seeds as well as other crop seeds.展开更多
The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which ...The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which may be attributed to the fact that small starch particles increase the filling capacity within the dough matrix.This increase subsequently resulted in more complete and uniform structure of dough.Moreover,setback viscosity rose to 1095 cP.The rapid water absorption of coix seed starch led to changes in the secondary structure of proteins in dough.The proportion ofβ-sheet markedly increased,whereas the proportion ofα-helix decreased with increasing level of coix seed flour substitution.Meanwhile,coix seed flour substitution significantly reduced the estimated glycemic index of noodles.Correlation analysis revealed that pasting parameters had significant effects on the evaluation of noodle quality.This study provides a foundation for researchers to substitute wheat flour with coix seed flour.展开更多
【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact ...【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.展开更多
文摘探讨基于“三全育人”教育理念的护理本科生SEED数字化学风建设,即以学风(style of study)建设为核心,教育(education)为驱动,在线学习(e-learning)为衔接,数字化(digitization)手段为支撑。通过数字化技术整合教育资源,优化学生的学习行为,推动全员参与、全过程管理和全方位覆盖的学风建设模式,以提升护理本科生的学习投入度和信息素养为主要目标,为护理教育提供新的实践路径。
文摘Salinity is one of the major abiotic stresses limiting chickpea(Cicer arietinum L.)productivity,particularly in arid and semi-arid regions where soil salinization is intensifying.Developing cost-effective and practical strategies to enhance seedling establishment and early vigor under saline conditions is therefore essential.In this study,we compared two seed-priming agents-1 mM proline and 25 mM NaCl-under identical hydroponic conditions to elucidate tissue-specific responses to 25 mM NaCl stress.Proline priming significantly improved shoot length(by~23%),total chlorophyll content(by~19%),and ascorbate peroxidase(ASPOX)activity.In contrast,NaCl priming enhanced root biomass retention(by~38%)and peroxidase(POD)activity under salinity stress.Both priming treatments induced higher proline accumulation and antioxidant capacity,though with tissue-specific effects:proline favored aboveground resilience,while NaCl strengthened root ionic and oxidative balance.These findings highlight the complementary nature of proline and NaCl priming and support the concept of stress“memory,”whereby plants acquire enhanced readiness to cope with salinity.Integrating such priming strategies into chickpea cultivation could contribute to improved yield stability and sustainability in saline agroecosystems.
文摘Date palm(Phoenix dactylifera L.)seeds,often discarded as by-products during date fruit processing,represent approximately 6-20%of the fruit’s mass depending on cultivar and maturity stage.Although traditionally used for producing activated charcoal and in environmental remediation,their pharmacological potential remains underexplored.This study aimed to evaluate the chemical composition,biological activity,and potential commercial value of seeds from four widely consumed Saharan cultivars(Assiane,Boufegouss,Aziza,and Majhoul)collected in the Figuig region.Soxhlet extraction was employed to obtain seed extracts,which were subsequently characterized by qualitative and quantitative phytochemical screening.The analyses revealed the presence of polyphenols,flavonoids,terpenoids,steroids,alkaloids,and other secondary metabolites.The Aziza and Boufegouss varieties were particularly rich in flavonoids(82.09-139.61 mg quercetin equivalents/100 g dry matter)and total polyphenols(69.81-1183.96 mg gallic acid equivalents/100 g dry matter).Mineral profiling confirmed the occurrence of essential trace elements,including Zn,Cu,Fe,and Co.To investigate their antidiabetic potential,in vitro assays ofα-glucosidase andα-amylase inhibition were performed.Acetone extracts demonstrated significant inhibitory activity against both enzymes,suggesting a promising role of P.dactylifera seed extracts as natural agents for the management of type 2 diabetes mellitus.
基金supported by Biological Breeding-National Science and Technology Major Project(2022ZD04008)National Natural Science Foundation of China(32201854)。
文摘Thousand-seed weight(TSW)is a critical target for genetic improvement in rapeseed(Brassica napus L.).However,phenotypic selection for this trait remains challenging due to its polygenic regulation by multiple quantitative trait loci(QTL).Here,six favorable TSW QTL alleles from two donor parents were introgress into an elite restorer line,621R,using an integrated strategy combining marker-assisted backcrossing and speed breeding protocols.Through six rounds of backcrossing and convergent crossing followed by two generations of selfing strategies,we developed 13 advanced lines with diverse TSW QTL combinations within 24 months.Field evaluations across three environments revealed that all lines exhibited significantly increased TSW in spring conditions(Minle,Gansu)and winter environments(Wuhan and Jiangling,Hubei)except for two lines which only showed increase in the spring environment.Hybridization assays using these lines as male parents crossed with two male-sterile lines(RG430A and 616A)demonstrated transgressive segregation for TSW:For RG430A-derived hybrids,all crosses significantly outperformed the original control(RG430A×621R)in Wuhan,with 8/13 and 9/13 crosses showing significant TSW increases in Minle and Jiangling,respectively.For 616A-derived hybrids,11/13 and 10/13 crosses exhibited significant TSW enhancement in Minle and Jiangling,compared to 3/13 in Wuhan.Notably,two top-performing hybrids achieved 13.0%and 6.8%higher plot yields,respectively.Our results demonstrate that strategic pyramiding of complementary TSW QTL alleles effectively enhances seed weight in rapeseed,and these improved lines represent valuable genetic resources for developing high-yield hybrids.
基金supported by the Jilin Science and Technology Development Program,China (20240602032RC)the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZD001)+1 种基金the Jilin Agricultural Science and Technology Innovation Project,China (CXGC2024ZY012)the Jilin Province Development and Reform Commission-Project for Improving the Independent Innovation Capacity of Major Grain Crops,China (2024C002)。
文摘Emerging and powerful genome editing tools,particularly CRISPR/Cas9,are facilitating functional genomics research and accelerating crop improvement(Jiang et al.2021;Cao et al.2023;Chen C et al.2023;Liu et al.2023a).However,the detection and screening of transgenic lines remain major bottlenecks,being time-consuming,labor-intensive,and inefficient during transformation and subsequent mutation identification.A simple and efficient visual marker system plays a critical role in addressing these challenges.Recent studies demonstrated that the GmW1 and RUBY reporter systems were used to obtain visual transgenic soybean(Glycine max) plants(Chen L et al.2023;Chen et al.2024).
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.82573747,82172873,W2421095,and 82503888)National Science and Technology Major Project(Grant No.2025ZD0543900)+2 种基金Natural Science Foundation of Shandong Province(Grant Nos.ZR2024LMB011 and ZR2024QH058)Taishan Scholars Program of Shandong Province(Grant No.tsqn202211337)Collaborative Academic Innovation Project of Shandong Cancer Hospital(Grant No.GF003).
文摘Neoadjuvant therapy(NAT)has become the standard treatment for patients with locally advanced breast cancer and stage II-III HER2-positive(HER2+)or triple-negative breast cancer(TNBC)1,2.It is essential to accurately mark the primary breast tumor and positive axillary lymph nodes(ALNs)prior to NAT to ensure precise surgical excision,guide axillary downstaging,and guarantee reliable lesion retrieval for pathologic evaluation3.The false-negative rate of sentinel lymph node biopsy(SLNB)after NAT can be reduced to<10%by applying modalities,such as the identification of≥3 sentinel lymph nodes(SLNs)with dual-mapping techniques or removal of the marked lymph node with target axillary dissection(TAD)according to the ASCO,NCCN,and CBCS guidelines3-5.However,there is a lack of consensus regarding the optimal methods and materials for accurate marking6,7.Conventional techniques include clip placement,guidewire localization,and carbon or ink tattooing,whereas wireless technologies,such as MagseedR,radiofrequency identification tags,SAVI SCOUTR,and radioactive iodine-125(125I)seeds,have also been adopted.Traditional marking techniques have a localization failure rate of approximately 10%.In contrast,the use of 125I seeds(with a radiation dose of 0.1-0.3 mCi)has significantly improved localization accuracy8,9.Nevertheless,owing to radioactive properties,concerns have been raised regarding the potential impact of 125I seed marking on assessing the pathologic complete response(pCR)after NAT10.Moreover,whether the influence of 125I seed marking on pCR could lead to suboptimal adjuvant treatment decisions and potentially compromise long-term oncologic outcomes has not been established.To investigate the potential impact of 125I seed placement on the pCR rate and long-term outcomes in breast cancer patients receiving NAT,we conducted a retrospective cohort study utilizing propensity score matching(PSM).
基金supported by the National Natural Science Foundation of China (Nos.81973410 and 82473537)the Independent Research Fund of Yunnan Characteristic Plant Extraction Laboratory (Nos.2022YKZY002 and 2022YKZY004)。
文摘Nigella sativa L.seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo.This study revealed that the ethanolic extract of Nigella sativa L.(HZC)enhances melanogenesis and mitigates oxidative stress-induced cellular senescence and dysfunction in melanocytes.In accordance with established protocols,the ethanol fraction from Nigella sativa L.seeds was extracted,concentrated,and lyophilized to evaluate its herbal effects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays,tyrosinase activity evaluation,measurement of cellular melanin contents,scratch assays,senescence-associatedβ-galactosidase(SA-β-gal)staining,enzyme-linked immunosorbent assay(ELISA),and Western blot analysis for expression profiling of experimentally relevant proteins.The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr,Mitf,and gp100 in B16F10 cells.Furthermore,HZC effectively mitigated oxidative stress-induced cellular senescence,improved melanocyte condition,and rectified various functional impairments associated with melanocyte dysfunction.These findings suggest that HZC increases melanin synthesis in melanocytes through the activation of the MAPK,PKA,and Wnt signaling pathways.In addition,HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuclear factor E2-related factor 2-antioxidant response element(Nrf2-ARE)pathway and enhancing the activity of downstream antioxidant enzymes,thus preventing premature senescence and dysfunction in melanocytes.
基金supported by Natural Science Foundation of Heilongjiang Province of China(SS2021C005)Province Key Research and Development Program of Heilongjiang Province of China(GZ20220121)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.
文摘Rapid,accurate seed classification of soybean varieties is needed for product quality control.We describe a hyperspectral image-based deep-learning model called Dual Attention Feature Fusion Networks(DAFFnet),which sequentially applies 3D Convolutional Neural Network(CNN)and 2D CNN.A fusion attention mechanism module in 2D CNN permits the model to capture local and global feature information by combining with Convolution Block Attention Module(CBAM)and Mobile Vision Transformer(MViT),outperforming conventional hyperspectral image classification models in seed classification.
基金National Natural Science Foundation of China,No.81970529The Natural Science Foundation of Jilin Province,No.20230508074RC and No.YDZJ202401218ZYTS.
文摘Colorectal cancer(CRC)is the most prevalent cancer globally,and its traditional treatment modalities commonly encompass radiation therapy,chemotherapy,surgery and the administration of cytotoxic drugs.Currently,novel chemotherapy drugs that combine traditional Chinese medicine(TCM)with herbal extracts exhibit superior comprehensive benefits.Herein,we delved into an article authored by Wang et al,focusing specifically on the pharmacological effects of"Herba Patriniae and Coix seed(HC)"and their targeted mechanisms in combating CRC.From the perspective of TCM philosophy,damp-heat stagnation and toxicity are the cardinal pathogenic factors underlying CRC.HC,renowned for their abilities to antipyretic and enhance diuresis,have demonstrated promising efficacy in preliminary studies for the treatment of CRC.These findings offer potential insights in favor of fostering anti-cancer medications.
基金supported in part by the Six Talent Peaks Project in Jiangsu Province under Grant 013040315in part by the China Textile Industry Federation Science and Technology Guidance Project under Grant 2017107+1 种基金in part by the National Natural Science Foundation of China under Grant 31570714in part by the China Scholarship Council under Grant 202108320290。
文摘The cleanliness of seed cotton plays a critical role in the pre-treatment of cotton textiles,and the removal of impurity during the harvesting process directly determines the quality and market value of cotton textiles.By fusing band combination optimization with deep learning,this study aims to achieve more efficient and accurate detection of film impurities in seed cotton on the production line.By applying hyperspectral imaging and a one-dimensional deep learning algorithm,we detect and classify impurities in seed cotton after harvest.The main categories detected include pure cotton,conveyor belt,film covering seed cotton,and film adhered to the conveyor belt.The proposed method achieves an impurity detection rate of 99.698%.To further ensure the feasibility and practical application potential of this strategy,we compare our results against existing mainstream methods.In addition,the model shows excellent recognition performance on pseudo-color images of real samples.With a processing time of 11.764μs per pixel from experimental data,it shows a much improved speed requirement while maintaining the accuracy of real production lines.This strategy provides an accurate and efficient method for removing impurities during cotton processing.
基金supported by grants from the Basic Research Business Fees for Public Welfare in Xinjiang Autonomous Region(Mining candidate genes related to grape seed traits based on GWAS,KY2023028)the Xinjiang Uygur Autonomous Region Tianchi Talent-Young Doctor for Chuan Zhang(Revealing the domestication history of Xinjiang native grape varieties and genetic analysis of important agronomic traits)the Xinjiang Academy of Agricultural Sciences Youth Science and Technology Backbone Innovation Ability Training Project(xjnkq-2023006).
文摘Seedlessness has always been a valuable quality characteristic of edible grape varieties.Although the production of seedless grapes has been ongoing for decades,the genetic complexity of seedless grapes is not yet fully understood.Therefore,determining the genetic mechanisms and key regulatory genes of seedless grapes is of great significance for seedless grape breeding and meeting market demands.The emergence of high-throughput analysis software offers greater possibilities for mining genes related to plant organ development.Specifically,to mine a greater number of candidate genes related to grape seed traits,this study used the seed trait parameters analyzed by Tomato Analyzer as the target trait and then used a genome-wide association study(GWAS)to mine candidate genes.In the two-year analysis using principal component analysis(PCA),we extracted five principal components with a cumulative contribution rate of 96.586%.The cumulative contribution rate for component 1 reached 87.352%.Correlation analysis revealed correlation coefficients ranging from 0.54 to 0.98 among the seven basic traits.The GWAS results indicated that 370 SNP loci were significantly correlated with seed traits.These SNP loci were distributed on 18 chromosomes,except for chromosome 4,with most SNP loci distributed on chromosome 18.Based on the physical location of single nucleotide polymorphism(SNP)markers significantly associated with seed-related traits in the grape reference genome,candidate genes are screened within the range of linkage disequilibrium(LD)attenuation distance,both upstream and downstream of the significant SNP loci.These candidate genes were mainly transcription factor-related genes(VvMADS4 and VvMADS5),ubiquitin ligase-related genes(E3 ubiquitin ligase BIG BROTHER),serine/threonine protein kinase-related genes,and carbohydrate metabolism-related genes(Sucrose Synthase 2)and simultaneously controlled multiple(at least two or more)seed traits.These results indicate that seed traits are jointly regulated by some genes involved in seed morphology regulation.In this work,we identified new gene loci related to grape seed traits.Identifying molecular markers closely related to these seed traits is of great significance for breeding seedless grape varieties.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502 to H.S.)the Yunnan Applied Basic Research Project(202001AT070060 to J.G.C.)+1 种基金the CAS“Light ofWest China”Program(J.G.C.)the Young Academic and Technical Leader Raising Foundation of Yunnan Province(202205AC160053 to J.G.C.).
文摘Population shrinkage of alpine cushion plants with ongoing climate warming has been empirically confirmed.Since cushion plants play important roles in sustaining alpine plant community and ecosystem functions,their population dynamics may directly influence the future alpine ecosystems.However,little is known about how climate warming affect cushion population recruitment,especially at early life-history stages.In this study,we conducted a laboratory simulation of climate warming to detect the effects of warming temperature and associated moisture and light changes on seed germination and seedling growth of the typical alpine cushion plant Arenaria oreophila.Results suggested that increasing temperature indeed exerted strong constraints on the population recruitment processes.Specifically,increased temperatures could quickly initialize seed germination(4e6 days at higher temperatures vs.29 e32 days at low temperature,respectively,after sowing),accelerate them to reach the maximum germination percentage(9e19 days at higher temperatures vs.57e86 days at low temperature,respectively,after sowing)and significantly accelerate seedling growth rate.However,higher temperatures accelerated seedling mortality(more than 80%).In addition,lower light availability also increased seedling mortality though it could generally increase the final seed germination percentage.The effects of water might be dependent on temperature and light.All results suggested that cushion A.oreophila is quite sensitive to climate warming which strongly constrains its seedling establishment process.We,therefore,speculate that the continuing climate warming in future will exert uncertain risks in the persistence of cushion A.oreophila,possibly by constraining the process of seedling recruitments.
基金financially supported by the Science and Technology Research of Shaanxi Province(2020QFY08-03)Forestry Science and Technology Programs of Shaanxi Province(SXLK2020-0213)supported by the Fundamental Research Funds for the Central Universities(GK202103098)。
文摘The high incidence of postpartum hypogalactia hinders the healthy development of postpartum women and the next generation.Lacquer seed oil(LSO),extracted from the seeds of the lacquer tree,has been traditionally used as a dietary supplement for promoting postpartum lactation and recovery in some districts of China.However,its physiological effects have not been verified,and the mechanism and active components of LSO have not been analyzed.Thus,we applied LSO to bromocriptine-induced postpartum hypogalactia rats.The results showed that LSO supplement effectively improves bromocriptine-induced postpartum hypogalactia.LSO also increased prolactin levels reduced by bromocriptine,promoted JAK2/STAT5 and PI3K/AKT pathways and several gene expression levels of milk synthesis in mammary gland.Moreover,metabolomic and network pharmacological analysis further revealed that JAK2/STAT5,PI3K/AKT,and estrogen signaling pathway are the potential main regulatory sites for the beneficial effects of LSO on postpartum hypogalactia,and that quercetin,kaempferol,arachidonic acid,epicatechin,and β-sitosterol are the top 5 main active ingredients of LSO.Our results suggested that LSO has great potential in the application of the improvement of postpartum hypogalactia.
基金supported by grants from the National Science Foundation of China(Grant Nos.U20A2049 and 32271922)。
文摘Torreya grandis'Merrillii'is a well-known nut in South China with high nutritional value.Severe premature seed abscission limits the industrial development of T.grandis by causing serious economic losses.However,the physiological mechanisms of seed abscission in T.grandis remain poorly understood.To gain insight into the relationships between carbohydrate status and seed abscission,three-year-old seed-bearing branches were taken as representative materials for the entire tree.Furthermore,the time course of changes in the photosynthetic rate and the non-structural carbohydrate(NSC)dynamics were monitored in the main sources(the one-year-old and two-year-old shoots),and the dry weight and NSC levels of sinks(the seeds,current female cone cluster,and current vegetative cluster)across all seed development stages were recorded.The cumulative seed abscission rate significantly increased,reaching 91.5%from 0 to 72 days after seed protrusion time(SPT).NSC levels in the main sources significantly decreased by 56%-79%,accompanied by a significantly increased photosynthesis rate of 17.1%-49.1%during that period and increased NSC levels in the three sinks.The gene expression level of cell wall invertase(TgCWIN)was significantly correlated with sucrose,fructose,and glucose levels.The carbon storage capacity of the main sources significantly decreased from 6.03 to3.14 mmol C·d^(-1),with a stable photosynthetic capacity,from 0 to 72 days after SPT,whereas the carbon demand of the three sinks showed a continuously increasing trend from 3.14 to 7.71 mmol C·d^(-1).In addition,sucrose supplementation significantly decreased the cumulative seed abscission rate.These results suggest that storage carbohydrates play a major role in the regulatory mechanism of seed abscission in T.grandis.Our study provides a theoretical basis for improving T.grandis yield through establishing a better carbon balance between sources and sinks using timely fertilization or proper pruning procedures.
基金supported by the National Natural Science Foundation of China(32072126 and 32230075)the Shandong Provincial Natural Science Foundation(ZR2019MC005).
文摘Pentatricopeptide repeat(PPR)proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression.Here,we report the function of PPR21 in mitochondrial intron splicing and its role in maize kernel development.PPR21 is a typical P-type PPR protein targeted to mitochondria.The ppr21 mutants are arrested in embryogenesis and endosperm development,leading to embryo lethality.Null mutations of PPR21 reduce the splicing efficiency of nad2 intron 1,2,and 4 and impair the assembly and activity of mitochondrial complex I.Previous studies show that the P-type PPR protein EMP12 is required for the splicing of identical introns.However,our protein interaction analyses reveal that PPR21 does not interact with EMP12.Instead,both PPR21 and EMP12 interact with the small MutS-related(SMR)domain-containing PPR protein 1(PPR-SMR1)and the short P-type PPR protein 2(SPR2).PPR-SMR1 interacts with SPR2,and both proteins are required for the splicing of many introns in mitochondria,including nad2 intron 1,2,and 4.These results suggest that a PPR21-(PPR-SMR1/SPR2)-EMP12 complex is involved in the splicing of nad2 introns in maize mitochondria.
基金supported by the Science and Technology Development Plan Project of Jilin Provincial Department of Science and Technology (No.20220203112S)the Jilin Provincial Department of Education Science and Technology Research Project (No.JJKH20210039KJ)。
文摘In this study,eight different varieties of maize seeds were used as the research objects.Conduct 81 types of combined preprocessing on the original spectra.Through comparison,Savitzky-Golay(SG)-multivariate scattering correction(MSC)-maximum-minimum normalization(MN)was identified as the optimal preprocessing technique.The competitive adaptive reweighted sampling(CARS),successive projections algorithm(SPA),and their combined methods were employed to extract feature wavelengths.Classification models based on back propagation(BP),support vector machine(SVM),random forest(RF),and partial least squares(PLS)were established using full-band data and feature wavelengths.Among all models,the(CARS-SPA)-BP model achieved the highest accuracy rate of 98.44%.This study offers novel insights and methodologies for the rapid and accurate identification of corn seeds as well as other crop seeds.
文摘The effect of coix seed flour(0%–25%)on the rheological and structural properties of wheat dough and noodle quality was investigated.The addition of coix seed flour was found to enhance the elasticity of dough,which may be attributed to the fact that small starch particles increase the filling capacity within the dough matrix.This increase subsequently resulted in more complete and uniform structure of dough.Moreover,setback viscosity rose to 1095 cP.The rapid water absorption of coix seed starch led to changes in the secondary structure of proteins in dough.The proportion ofβ-sheet markedly increased,whereas the proportion ofα-helix decreased with increasing level of coix seed flour substitution.Meanwhile,coix seed flour substitution significantly reduced the estimated glycemic index of noodles.Correlation analysis revealed that pasting parameters had significant effects on the evaluation of noodle quality.This study provides a foundation for researchers to substitute wheat flour with coix seed flour.
文摘【Background】The application of beneficial-microbial seed soaking prior to sowing represents a novel technology that has not been employed in Lanzhou lily cultivation.We conducted an experiment to explore the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology(NGS).【Methods】Lily bulbs were soaked in a seed treating agent containing beneficial microbes(SP treatment)for 4 hours.Subsequently,they were planted in soil in July and sampled in September to assess plant growth,rhizosphere soil physicochemical properties,and microorganism community structures.In addition,we employed the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.【Results】Under SP treatment,there were significant alterations in fungi and bacteria community structures,accompanied by improved soil nutrient status.Notably,the relative abundance of dominant microorganism groups,such as the fungi Basidiomycota,Pseudeurotium,Cladophialophora,Microascus,and Dactylonectria,as well as the bacteria Proteobacteria,Chloroflexi,Ochrobactrium,Lysobacter,and RB41,underwent notable changes.Microorganism function prediction results indicated a reduction in pathotrophic fungi(including plant pathogens)and an increase in endophytic and saprotrophic fungi under SP treatment.Among the top 20 metabolism pathways,80%were upregulated in SP treatment compared to the CK.【Conclusions】Seed soaking with beneficial microbial strain promotes the growth of Lanzhou lily bulbs.The beneficial microorganisms play a crucial role in regulating soil microbial structures,enhancing the accumulation of endophytic fungi,reducing the abundance of pathogens,and improving soil functions.Furthermore,specific microbial groups are found to be involved in maintaining soil health.
