Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unpreceden...Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unprecedented unsaturated lin-ear polyketides with an epoxy ring structure.The structures,including absolute configurations of these compounds,were elucidated through detailed analyses of nuclear magnetic resonance(NMR)and high-resolution mass spectrometry(HR-MS)data,as well as elec-tronic custom distributors(ECD)calculations.In the cytotoxicity screening against prostate cancer cell lines,talaketide E(5)demon-strated a dose-dependent inhibitory effect on prostate cancer PC-3 cell lines,with an IC50 value of 14.44 μmol·L-1.Moreover,com-pound 5 significantly inhibited the cloning formation of PC-3 cell lines and arrested the cell cycle in S-phase,ultimately inducing ap-optosis.These findings indicate that compound 5 may serve as a promising lead compound for the development of a potential treat-ment for prostate cancer.展开更多
The global intensification of harmful algal blooms severely compromises freshwater ecosystems,threatening biodiversity and critical ecosystem services through toxin exposure,hypoxia,and water quality degradation.Bloom...The global intensification of harmful algal blooms severely compromises freshwater ecosystems,threatening biodiversity and critical ecosystem services through toxin exposure,hypoxia,and water quality degradation.Bloom formation involves a complex interplay of nutrient dynamics,hydrology,and microbial activity.Although subsurface processes-such as the release of sediment-bound nutrients and the germination of dormant cyanobacteria-are thought crucial to bloom initiation,these phenomena occur at fine spatiotemporal scales beyond the reach of conventional monitoring.As a result,the exact,rapidly evolving triggers of bloom emergence remain mostly unknown.Here we show meter-scale chlorophyll a(Chl-a)plumes rising from the sediment-water interface,triggered by heavy rainfall and directly seeding surface blooms.We captured these dynamics using a custom underwater drone that collected over 2.8 million data points at 5-m horizontal and 1-m vertical resolution.Algal blooms exhibit a clear vertical sequence:anomalous Chl-a levels first appear in deep benthic layers after rainfall-driven resuspension,then intensify simultaneously across near-bed depths,and finally reach the surface after a median lag of 0.8-1.5 days.These observations provide in situ evidence associating benthic algal seed stocks with surface bloom initiation,revealing that the origin and spatial heterogeneity of such events arise from rainfall-driven disturbances at the sediment-water interface.This robotic approach not only deciphers the subsurface origins of algal blooms but also empowers predictive modeling and adaptive management strategies,advancing global efforts to combat eutrophication amid escalating climate pressures and safeguard vital water resources.展开更多
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
基金supported by the Key-Area Research and Development Program of Guangdong Province(No.2023B1111050008)the National Natural Science Foundation of China(Nos.U23A20528,U20A20101)+1 种基金Guangdong Local Innovation Team Program(No.2019BT02Y262)the Postdoctoral Fellowship Program of CPSF(No.GZC20232777).
文摘Seven novel linear polyketides,talaketides A-G(1-7),were isolated from the rice media cultures of the mangrove sed-iment-derived fungus Talaromyces sp.SCSIO 41027.Among these,talaketides A-E(1-5)represented unprecedented unsaturated lin-ear polyketides with an epoxy ring structure.The structures,including absolute configurations of these compounds,were elucidated through detailed analyses of nuclear magnetic resonance(NMR)and high-resolution mass spectrometry(HR-MS)data,as well as elec-tronic custom distributors(ECD)calculations.In the cytotoxicity screening against prostate cancer cell lines,talaketide E(5)demon-strated a dose-dependent inhibitory effect on prostate cancer PC-3 cell lines,with an IC50 value of 14.44 μmol·L-1.Moreover,com-pound 5 significantly inhibited the cloning formation of PC-3 cell lines and arrested the cell cycle in S-phase,ultimately inducing ap-optosis.These findings indicate that compound 5 may serve as a promising lead compound for the development of a potential treat-ment for prostate cancer.
基金supported by the National Natural Science Foundation of China(No.52321005,No.52293443,and No.52230004)Shenzhen Science and Technology Program(No.KQTD20190929172630447)+2 种基金Shenzhen Key Research Project(No.GXWD20220817145054002)Shenzhen Natural Science Foundation(No.JCYJ20240813104812017)Talent Recruitment Project of Guangdong(No.2021QN020106).
文摘The global intensification of harmful algal blooms severely compromises freshwater ecosystems,threatening biodiversity and critical ecosystem services through toxin exposure,hypoxia,and water quality degradation.Bloom formation involves a complex interplay of nutrient dynamics,hydrology,and microbial activity.Although subsurface processes-such as the release of sediment-bound nutrients and the germination of dormant cyanobacteria-are thought crucial to bloom initiation,these phenomena occur at fine spatiotemporal scales beyond the reach of conventional monitoring.As a result,the exact,rapidly evolving triggers of bloom emergence remain mostly unknown.Here we show meter-scale chlorophyll a(Chl-a)plumes rising from the sediment-water interface,triggered by heavy rainfall and directly seeding surface blooms.We captured these dynamics using a custom underwater drone that collected over 2.8 million data points at 5-m horizontal and 1-m vertical resolution.Algal blooms exhibit a clear vertical sequence:anomalous Chl-a levels first appear in deep benthic layers after rainfall-driven resuspension,then intensify simultaneously across near-bed depths,and finally reach the surface after a median lag of 0.8-1.5 days.These observations provide in situ evidence associating benthic algal seed stocks with surface bloom initiation,revealing that the origin and spatial heterogeneity of such events arise from rainfall-driven disturbances at the sediment-water interface.This robotic approach not only deciphers the subsurface origins of algal blooms but also empowers predictive modeling and adaptive management strategies,advancing global efforts to combat eutrophication amid escalating climate pressures and safeguard vital water resources.
